Objective This study was made to evaluate the effect of acupuncture on fertilization results in the patients undergoing medical treatment with IVF. Methods This study was conducted on 164 infertile patients who had re...Objective This study was made to evaluate the effect of acupuncture on fertilization results in the patients undergoing medical treatment with IVF. Methods This study was conducted on 164 infertile patients who had referred to the infertility clinic of Imam Khomeini Hospital in 2009–2010（82 patients in acupuncture group and 82 in control group）. In the acupuncture group, before embryo transfer on oocyte puncture day, 2 days after puncture and one cycle before embryo transfer the patients were put under electroacupuncture on the Bǎihuì（百会 GV 20）, Yāoyángguān（腰阳关 CV 3）, Tàichōng（太冲 LR 3）, Nèiguān（内关 PC 6）, Sānyīnjiāo（三阴交 SP 6）, Guīlái（归来 ST 29）, Zúsānlǐ（足三里 ST 36）, and Hégǔ（合谷 LI 4） for three times each for 25 minutes. The points on ears include nèi Shēngzhíqì（内生殖器 TF 2）, and Shénmén（神门 TF 4）. The control group received no acupuncture. Positive pregnancy test, clinical pregnancy, ongoing pregnancy were compared between the two groups. Results Pregnancy level in the acupuncture group were 34 pregnancies（41.5%） as compared to 21（25.6%） in the control group（P〈0.05）.Observation level of gestational sacs in the 5th week of pregnancy included 31（37.8%） and 17（20.7%） sacs in the acupuncture and control groups, respectively（P〈0.05）.Level of ongoing pregnancies after 12 weeks included 21（21.6%） and 12（14.6%） pregnancies in the acupuncture and control groups, respectively（P〉0.05）. Conclusion Acupuncture will considerably improve the result of IVF as compared to the control group.展开更多
目的:探讨毓麟珠方联合调和阴阳针刺法治疗波塞冬第四分组卵巢储备功能下降患者体外受精-胚胎移植(Invitro fertilization and embryo transfer,IVF-ET)妊娠结局临床观察。方法:选择于华中科技大学同济医学院附属武汉中西医结合医院就...目的:探讨毓麟珠方联合调和阴阳针刺法治疗波塞冬第四分组卵巢储备功能下降患者体外受精-胚胎移植(Invitro fertilization and embryo transfer,IVF-ET)妊娠结局临床观察。方法:选择于华中科技大学同济医学院附属武汉中西医结合医院就诊的欲行IVF-ET的波塞冬第四分组卵巢储备功能下降患者62例,根据随机数字表法分为观察组与对照组各31例,两组均给予地屈孕酮片治疗,对照组给予调和阴阳针刺法,观察组在对照组基础上给予毓麟珠方治疗,观察两组治疗前后临床疗效、中医证候评分、血清细胞因子[即细胞间黏附分子-1(ICAM-1)、环氧合酶-2(COX-2)]浓度水平、血清生殖激素水平;B超观察基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速,促排情况(Gn天数、Gn剂量、获卵数、MII卵数、获卵率、MII卵率)、评价IVF-ET结局指标(胚胎种植率、临床妊娠率、活产率)及安全性。结果:观察组治疗后总有效率为90.32%,显著高于对照组的64.52%(P<0.05);与治疗前比较,治疗后两组中医证候评分、血清促卵泡激素(FSH)、血清ICAM-1、COX-2含量降低(P<0.05);治疗后观察组中医证候评分、血清FSH、HG活力、血清ICAM-1、COX-2含量低于对照组(P<0.05);治疗后观察组血清LH水平低于对照组(P<0.05);与治疗前比较,治疗后两组基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速明显升高(P<0.05);治疗后观察组基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速高于对照组(P<0.05);观察组促排后的获卵数、获卵率、MII卵数、MII卵率均高于对照组(P<0.05)。结论:毓麟珠方联合调和阴阳针刺法治疗波塞冬第四分组卵巢储备功能下降患者临床疗效显著。展开更多
目的:从开菲尔粒和陈年泡菜水中分离出具有降胆固醇能力的乳酸杆菌,探讨其降解小鼠血清胆固醇的机理。方法:以耐酸性、胆盐耐受性、疏水性、胆盐水解酶活性以及降胆固醇特性筛选出1株性状优良的植物乳杆菌(Lactobacillus plantarum DMDL...目的:从开菲尔粒和陈年泡菜水中分离出具有降胆固醇能力的乳酸杆菌,探讨其降解小鼠血清胆固醇的机理。方法:以耐酸性、胆盐耐受性、疏水性、胆盐水解酶活性以及降胆固醇特性筛选出1株性状优良的植物乳杆菌(Lactobacillus plantarum DMDL 9010)。将50只8周龄的Sprague Dawley大鼠随机分为正常组、模型组、阳性组、9010高组、9010低组,分别饲喂28 d和70 d采血,测定总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)的含量,70 d取肝组织细胞实时荧光逆转录聚合酶链式反应检测胆固醇合成限速酶3-羟基-3-甲基戊二酸单酰辅酶A还原酶(3-hydroxy-3-methylglutaryl-Co A reducase,HMGCR)的相对表达量。结果:筛选得到1株具有降胆固醇能力的L.plantarum DMDL 9010,胆固醇去除率为37.58%,胆盐耐受性为35.48%,疏水性高达40%,具有较好的耐酸性。饲喂第28天,成功建出高脂模型大鼠,饲喂第70天,9010高组能显著降低高脂大鼠血清TC(23.03%)和LDL-C(28.00%),9010低组和阳性组无明显差异。阳性组和9010高组分别下调肝脏中HMG-Co A基因m RNA的表达(79.92%和62.86%)(P<0.05)。结论:实验获得了1株在体内外均具有高效降胆固醇能力的L.plantarum DMDL 9010,可进一步开发为功能性微生态制剂。展开更多
文摘Objective This study was made to evaluate the effect of acupuncture on fertilization results in the patients undergoing medical treatment with IVF. Methods This study was conducted on 164 infertile patients who had referred to the infertility clinic of Imam Khomeini Hospital in 2009–2010（82 patients in acupuncture group and 82 in control group）. In the acupuncture group, before embryo transfer on oocyte puncture day, 2 days after puncture and one cycle before embryo transfer the patients were put under electroacupuncture on the Bǎihuì（百会 GV 20）, Yāoyángguān（腰阳关 CV 3）, Tàichōng（太冲 LR 3）, Nèiguān（内关 PC 6）, Sānyīnjiāo（三阴交 SP 6）, Guīlái（归来 ST 29）, Zúsānlǐ（足三里 ST 36）, and Hégǔ（合谷 LI 4） for three times each for 25 minutes. The points on ears include nèi Shēngzhíqì（内生殖器 TF 2）, and Shénmén（神门 TF 4）. The control group received no acupuncture. Positive pregnancy test, clinical pregnancy, ongoing pregnancy were compared between the two groups. Results Pregnancy level in the acupuncture group were 34 pregnancies（41.5%） as compared to 21（25.6%） in the control group（P〈0.05）.Observation level of gestational sacs in the 5th week of pregnancy included 31（37.8%） and 17（20.7%） sacs in the acupuncture and control groups, respectively（P〈0.05）.Level of ongoing pregnancies after 12 weeks included 21（21.6%） and 12（14.6%） pregnancies in the acupuncture and control groups, respectively（P〉0.05）. Conclusion Acupuncture will considerably improve the result of IVF as compared to the control group.
文摘目的:探讨毓麟珠方联合调和阴阳针刺法治疗波塞冬第四分组卵巢储备功能下降患者体外受精-胚胎移植(Invitro fertilization and embryo transfer,IVF-ET)妊娠结局临床观察。方法:选择于华中科技大学同济医学院附属武汉中西医结合医院就诊的欲行IVF-ET的波塞冬第四分组卵巢储备功能下降患者62例,根据随机数字表法分为观察组与对照组各31例,两组均给予地屈孕酮片治疗,对照组给予调和阴阳针刺法,观察组在对照组基础上给予毓麟珠方治疗,观察两组治疗前后临床疗效、中医证候评分、血清细胞因子[即细胞间黏附分子-1(ICAM-1)、环氧合酶-2(COX-2)]浓度水平、血清生殖激素水平;B超观察基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速,促排情况(Gn天数、Gn剂量、获卵数、MII卵数、获卵率、MII卵率)、评价IVF-ET结局指标(胚胎种植率、临床妊娠率、活产率)及安全性。结果:观察组治疗后总有效率为90.32%,显著高于对照组的64.52%(P<0.05);与治疗前比较,治疗后两组中医证候评分、血清促卵泡激素(FSH)、血清ICAM-1、COX-2含量降低(P<0.05);治疗后观察组中医证候评分、血清FSH、HG活力、血清ICAM-1、COX-2含量低于对照组(P<0.05);治疗后观察组血清LH水平低于对照组(P<0.05);与治疗前比较,治疗后两组基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速明显升高(P<0.05);治疗后观察组基础窦卵泡数、卵巢动脉收缩期峰值流速、卵巢动脉舒张末期流速高于对照组(P<0.05);观察组促排后的获卵数、获卵率、MII卵数、MII卵率均高于对照组(P<0.05)。结论:毓麟珠方联合调和阴阳针刺法治疗波塞冬第四分组卵巢储备功能下降患者临床疗效显著。
