Objective:To investigate the expression of targeting protein for Xenopus kinesin-like protein 2(TPX2) in breast cancer tissue and to explore its role in proliferation,migration and invasion of breast cancer cells.Meth...Objective:To investigate the expression of targeting protein for Xenopus kinesin-like protein 2(TPX2) in breast cancer tissue and to explore its role in proliferation,migration and invasion of breast cancer cells.Methods:The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot.The effect of TPX2 with RNA interference on proliferation,invasion and migration of breast cancer cells was observed by MTT and Transwell assays.Results:Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue.TPX2 expression was also upregulated in breast cancer cell lines,and the TPX2 interfered by small interfering RNA could inhibit the proliferation,invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9.Conclusions:Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells,and contributes to promote the proliferation,migration and invasion of breast cancer cells.展开更多
目的探讨廊管生成素样蛋白1（angiopoietin—like protein 1，ANGPTL1）在结直肠癌中表达的临床意义及其对结直肠癌细胞生物学行为的影响。方法应用免疫组织化学检测98例配对的结直肠癌和癌旁组织中ANGPTL1蛋白表达水平,用免疫印迹和实...目的探讨廊管生成素样蛋白1（angiopoietin—like protein 1，ANGPTL1）在结直肠癌中表达的临床意义及其对结直肠癌细胞生物学行为的影响。方法应用免疫组织化学检测98例配对的结直肠癌和癌旁组织中ANGPTL1蛋白表达水平,用免疫印迹和实时定量PCR检测24对新鲜结直肠癌和配对癌旁组织中ANGPTL1蛋白和mRNA表达。检测ANGPTL1在不同转移特性的结直肠癌细胞中的表达，以及ANGPTL1干扰对结直肠癌细胞侵袭和迁移的影响。结果ANGPTL1在结直肠癌组织中表达低于癌旁正常组织（44％比76％，P〈0．01）。ANGPTL1表达与结直肠癌患者T分期（χ^2=5．766，P=0．016）、淋巴结转移（χ^2=5．571，P=0．018）及TNM分期（χ^2=7．773，P=0．005）有关。单因素分析发现，ANGPTL1阳性表达的结直肠癌患者预后良好。ANGPTL1在结直肠癌组织中蛋白和mRNA表达均高于癌旁正常组织（t=3．126，P=0．005；t=2．523，P=0．019）。ANGPTL1干扰促进HCT116结直肠癌细胞侵袭和迁移力。结论ANGFFL1在结直肠癌中呈低表达．与肿瘤T分期、淋巴结转移、TNM分期及不良预后有关。ANGPTL1抑制结直肠癌细胞的侵袭和迁移。展开更多
基金Supported by National Science Foundation(81502322)
文摘Objective:To investigate the expression of targeting protein for Xenopus kinesin-like protein 2(TPX2) in breast cancer tissue and to explore its role in proliferation,migration and invasion of breast cancer cells.Methods:The mRNA and protein expressions of TPX2 in breast cancer tissue and cell lines were assessed by quantitative RT-PCR and Western blot.The effect of TPX2 with RNA interference on proliferation,invasion and migration of breast cancer cells was observed by MTT and Transwell assays.Results:Both mRNA and protein expressions of TPX2 were upregulated in breast cancer tissues compared to tumor-adjacent tissue.TPX2 expression was also upregulated in breast cancer cell lines,and the TPX2 interfered by small interfering RNA could inhibit the proliferation,invasion and migration of breast cancer cells by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9.Conclusions:Significantly upregulated TPX2 expression is observed in breast cancer tissue and cells,and contributes to promote the proliferation,migration and invasion of breast cancer cells.
文摘目的探讨廊管生成素样蛋白1（angiopoietin—like protein 1，ANGPTL1）在结直肠癌中表达的临床意义及其对结直肠癌细胞生物学行为的影响。方法应用免疫组织化学检测98例配对的结直肠癌和癌旁组织中ANGPTL1蛋白表达水平,用免疫印迹和实时定量PCR检测24对新鲜结直肠癌和配对癌旁组织中ANGPTL1蛋白和mRNA表达。检测ANGPTL1在不同转移特性的结直肠癌细胞中的表达，以及ANGPTL1干扰对结直肠癌细胞侵袭和迁移的影响。结果ANGPTL1在结直肠癌组织中表达低于癌旁正常组织（44％比76％，P〈0．01）。ANGPTL1表达与结直肠癌患者T分期（χ^2=5．766，P=0．016）、淋巴结转移（χ^2=5．571，P=0．018）及TNM分期（χ^2=7．773，P=0．005）有关。单因素分析发现，ANGPTL1阳性表达的结直肠癌患者预后良好。ANGPTL1在结直肠癌组织中蛋白和mRNA表达均高于癌旁正常组织（t=3．126，P=0．005；t=2．523，P=0．019）。ANGPTL1干扰促进HCT116结直肠癌细胞侵袭和迁移力。结论ANGFFL1在结直肠癌中呈低表达．与肿瘤T分期、淋巴结转移、TNM分期及不良预后有关。ANGPTL1抑制结直肠癌细胞的侵袭和迁移。
