The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and di...The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and different editions of Pharmacopoeia of People’s Republic of China.After textual research,Inulae Flos has been named as"Inulae Flos"since Shennong Bencaojing(Shennong’s Herbal Classic).Its original plant is Inula japonica Thunb.or I.britannica L.,which is easy to multiply and widely distributed.Inulae Flos is used medically with clean and dry capitulum,and there is also honey-burning method.The description of Inulae Flos in Pharmacopoeia of People’s Republic of China,such as nature and flavor,meridian,efficacy and indications,is generally consistent with the records in traditional herbal books.展开更多
Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.M...Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.Materials and Methods:Active ingredient library of Inulae Flos is structured using databases and the literature.“Compound-target-pathway”network on anti-hepatitis and protein–protein interaction(PPI)network are constructed using network pharmacology.Next,chromatographic fingerprints of Inulae Flos in 7 origins are obtained through HPLC,and chemometric analysis is implemented to identify chemical markers,which is combined with network pharmacology to identify Q-markers and detect content.Results:1,6-O,O-Diacetylbritannilactone,Ivangustin,and Inulanolide A are key ingredients of Inulae Flos to interact with 82 potential targets related to anti-hepatitis.Furthermore,signal transducer and activator of transcription 3,tumor necrosis factor,interleukin-6,and transcription factor AP-1 are the core targets in the PPI network.Chromatographic fingerprints of the Inulae Flos define 20 common peaks and identify 8 peaks using reference substances.Through partial least square discriminant analysis,7compounds including caffeic acid,chlorogenic acid,and 1,6-O,O-Diacetylbritannilactone were main chemical markers for variability.1,6-O,O-Diacetylbritannilactone is both a key ingredient and exclusive chemical marker.Therefore,1,6-O,O-diacetylbritannilactone is a Q-marker of Inulae Flos,and the average content is 1.82 mg/g.Conclusion:1,6-O,O-diacetylbritannilactone is determined to be a Q-marker of Inulae Flos.展开更多
In continuation of research aimed at identifying anti-inflammatory agents from natural sesquiterpenoids,an activity-guided fractionation approach utilizing lipopolysaccharide(LPS)-mediated RAW264.7 cells was employed ...In continuation of research aimed at identifying anti-inflammatory agents from natural sesquiterpenoids,an activity-guided fractionation approach utilizing lipopolysaccharide(LPS)-mediated RAW264.7 cells was employed to investigate chemical constituents from Inula Britannica(I.britannica).Seven novel sesquiterpenoid dimers inulabritanoids A−G(1−7)and two novel sesquiterpenoid monomers inulabritanoids H(8)and I(9)were isolated from I.britannica together with eighteen known compounds(10−27).The structural elucidation was accomplished through comprehensive analysis of 1D and 2D nuclear magnetic resonance(NMR),high-resolution mass spectrometry(HR-MS),and electronic circular dichroism(ECD)spectra,complemented by quantum chemical calculations.Compounds 1,2,12,16,19,and 26 demonstrated inhibitory effects on NO production,with IC50 values of 3.65,5.48,3.29,6.91,3.12,and 5.67μmol·L^(−1),respectively.Mechanistic studies revealed that compound 1 inhibited IκB kinaseβ(IKKβ)phosphorylation,thereby blocking nuclear factorκB(NF-κB)nuclear translocation,and activated the kelch-like ECH-associated protein 1(Keap1)/nuclear factor erythroid 2-related factor 2(Nrf2)signal pathway,leading to decreased expression of NADPH oxidase 2(NOX-2),inducible nitric oxide synthase(iNOS),tumor necrosis factorα(TNF-α),interleukin-6(IL-6),monocyte chemotactic protein-1(MCP-1),IL-1β,and IL-1αand increased expression of NAD(P)H:quinone oxidoreductase 1(NQO-1)and heme oxygenase-1(HO-1),thus exhibiting anti-inflammatory effects in vitro.These results indicate that dimeric sesquiterpenoids may serve as promising candidates for anti-inflammatory drug development.展开更多
以欧亚旋覆花(Inula britannica L.)无菌苗为材料,研究了不同浓度调吡脲(CPPU)对其叶片、根愈伤组织诱导的影响。结果显示,CPPU的浓度为0.10 mg/L时,叶片的愈伤组织诱导率为82.9%,且诱导出的愈伤组织生长好。CPPU的浓度为0.50 mg/L时,...以欧亚旋覆花(Inula britannica L.)无菌苗为材料,研究了不同浓度调吡脲(CPPU)对其叶片、根愈伤组织诱导的影响。结果显示,CPPU的浓度为0.10 mg/L时,叶片的愈伤组织诱导率为82.9%,且诱导出的愈伤组织生长好。CPPU的浓度为0.50 mg/L时,根的愈伤组织诱导率最高,达100.0%,愈伤组织生长最好,呈浅黄绿色,部分带有红色斑点,质地疏松,具光泽。展开更多
Three new anthranilic acid derivatives, N-heneicosanoylanthranilic acid （1b）, N-tricosanoylanthranilic acid （1d）, N-tetracosanoylanthranilic acid （1e）, and two known N-arachidylanthranilic acid （1a） and N-doco...Three new anthranilic acid derivatives, N-heneicosanoylanthranilic acid （1b）, N-tricosanoylanthranilic acid （1d）, N-tetracosanoylanthranilic acid （1e）, and two known N-arachidylanthranilic acid （1a） and N-docosanoylanthranilic acid （1c） were isolated from the aerial parts of lnula japonica Thunb. Their structures were established by spectroscopic and chemical methods.展开更多
Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spec...Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spectral methods.展开更多
The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatogr...The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography（HPLC） and infrared spectroscopy（IR） methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1：1：1.4：2.7：14.6：6.3：7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide（BCG-LPS）,not only the blood alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels decreased significantly（P0.05）,but aslo the liver tissue malondialdehyde（MDA） level decreased significantly（P0.01）.The superoxide dismutase（SOD） level increased significantly（P0.01）.IBP shows significant anti-liver injury and antioxidation activity.展开更多
Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and T...Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.展开更多
The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vit...The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.展开更多
Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of...Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC50 values ranging from 2.1 to 36.3 μM.展开更多
The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide（SF-CO 2） for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogen...The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide（SF-CO 2） for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide.The effects of factors,including extraction temperature,extraction pressure,SF-CO 2 flow rate,flower powder size,and time on the essential oil yield were studied using the single factor experiment.An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield.Adopting the optimum conditions,the maximum yield reached 10.01% at 40°C temperature,30 MPa pressure,60 mesh flower powder size,20 L h-1SF-CO 2 flow rate,and 90 min extraction time.The antifungal activities of I.britannica essential oil using the SF-CO 2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests.Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1concentration of the essential oil.The mycelial growth of Gaeumannomyces graminis var.tritici was completely inhibited.The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%,respectively.In addition,the essential oil can inhibit the spore germination of Fusarium oxysporum f.sp.vasinfectum,Phytophthora capsici,Colletotrichum orbiculare,and Pyricularia grisea,and the corresponding inhibition rates were 98.26,96.54,87.89,and 87.35% respectively.The present study has demonstrated that the essential oil of I.britannica flowers extracted through the SF-CO 2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.展开更多
Experimental assessments were made on the anti-HSV-Ⅱ action of 500 herbs by determinations of thevirus inhibition logarithm(VIL).13 highly effectiveherbs (VIL■4.00)were screened cut,providing a ra-tional basis for c...Experimental assessments were made on the anti-HSV-Ⅱ action of 500 herbs by determinations of thevirus inhibition logarithm(VIL).13 highly effectiveherbs (VIL■4.00)were screened cut,providing a ra-tional basis for clinical therapy.Among these effec-tive herbs,10 were aqueous extracts of Artemisiaanomala,Centella asiatica,Epimedium Sagittatum,Hibiscus mutabills,Hosta plantaginea,Hypericumjaponicum,Inula japonica.Mosla punctata,Rhodo-dendron simsii,and Rhus chinenses,while 3 werealcohol extracts of Epimedium Sagittatum,Hypericumjaponicum,and Mosla punctata.展开更多
The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening ...The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine(L-DOPA) staining was examined. The total flavonoid content(TFC) was increased by 13.4% after 72h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500μg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.展开更多
OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2&...OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2'-azinobis(3-ethylbenzothiazoline-6-suphonic acid)di-ammonium salt(ABTS),Folin-Ciocalteu and high-performance liquid chromatography(HPLC)methods.The effect against oxidative DNA base damage was determined analyzing oxidative DNA base damage products by GC-MS/MS.RESULTS:The methanol-water extract of Inula helenium root showed good antioxidant capacity[32.20 mg trolox equivalents(TE)/g dried weight(dw)by ABTS method,54.53 mg TE/g dw by chromium reducing antioxidant capacity method]and total phenolic content[29.83 mg gallic acid equivalent(GAE)/g dw].The chlorogenic acid,ferulic acid,rosmarinic acid and caffeic acid were determined in the extract by HPLC.The chlorogenic acid,ferulic acid and Inula helenium root extract were revealed to inhibit the oxidative DNA base damage by GC-MS/MS.CONCLUSION:The studies showed a crucial decrease in the amount of the DNA base damage products when antioxidants were used.The results showed that ferulic acid has better inhibition than chlorogenic acid for DNA oxidation.The methanol-water extract of Inula helenium root was determined to inhibit the oxidative DNA damage.展开更多
文摘The herbal textual research of Inulae Flos was carried out from the aspects of name,origin,habit and distribution,nature and flavor,meridian,efficacy and indications by referring to ancient Chinese herbal books and different editions of Pharmacopoeia of People’s Republic of China.After textual research,Inulae Flos has been named as"Inulae Flos"since Shennong Bencaojing(Shennong’s Herbal Classic).Its original plant is Inula japonica Thunb.or I.britannica L.,which is easy to multiply and widely distributed.Inulae Flos is used medically with clean and dry capitulum,and there is also honey-burning method.The description of Inulae Flos in Pharmacopoeia of People’s Republic of China,such as nature and flavor,meridian,efficacy and indications,is generally consistent with the records in traditional herbal books.
基金sponsored by National Key Research and Development Program of China(2018YFC1707000)the Project of the Industry University Research Cooperation and transformation of scientific and technological achievements in Qixia District of Nanjing。
文摘Objective:The objective of the study is to combine network pharmacology with high-performance liquid chromatography(HPLC)to screen for quality markers(Q-markers)of Inulae Flos and predict mechanism on anti-hepatitis.Materials and Methods:Active ingredient library of Inulae Flos is structured using databases and the literature.“Compound-target-pathway”network on anti-hepatitis and protein–protein interaction(PPI)network are constructed using network pharmacology.Next,chromatographic fingerprints of Inulae Flos in 7 origins are obtained through HPLC,and chemometric analysis is implemented to identify chemical markers,which is combined with network pharmacology to identify Q-markers and detect content.Results:1,6-O,O-Diacetylbritannilactone,Ivangustin,and Inulanolide A are key ingredients of Inulae Flos to interact with 82 potential targets related to anti-hepatitis.Furthermore,signal transducer and activator of transcription 3,tumor necrosis factor,interleukin-6,and transcription factor AP-1 are the core targets in the PPI network.Chromatographic fingerprints of the Inulae Flos define 20 common peaks and identify 8 peaks using reference substances.Through partial least square discriminant analysis,7compounds including caffeic acid,chlorogenic acid,and 1,6-O,O-Diacetylbritannilactone were main chemical markers for variability.1,6-O,O-Diacetylbritannilactone is both a key ingredient and exclusive chemical marker.Therefore,1,6-O,O-diacetylbritannilactone is a Q-marker of Inulae Flos,and the average content is 1.82 mg/g.Conclusion:1,6-O,O-diacetylbritannilactone is determined to be a Q-marker of Inulae Flos.
基金supported by the National Natural Science Foundation of China(Nos.82274069,82030116,and 82141212)the Young Scientific and Technological Talents(Level Two)in Tianjin(No.QN20230212)+1 种基金Tianjin Education Commission Research Program Project(No.2024KJ004)the Eaglet Plan Project of Tianjin University of Traditional Chinese Medicine(No.XJS2024101).
文摘In continuation of research aimed at identifying anti-inflammatory agents from natural sesquiterpenoids,an activity-guided fractionation approach utilizing lipopolysaccharide(LPS)-mediated RAW264.7 cells was employed to investigate chemical constituents from Inula Britannica(I.britannica).Seven novel sesquiterpenoid dimers inulabritanoids A−G(1−7)and two novel sesquiterpenoid monomers inulabritanoids H(8)and I(9)were isolated from I.britannica together with eighteen known compounds(10−27).The structural elucidation was accomplished through comprehensive analysis of 1D and 2D nuclear magnetic resonance(NMR),high-resolution mass spectrometry(HR-MS),and electronic circular dichroism(ECD)spectra,complemented by quantum chemical calculations.Compounds 1,2,12,16,19,and 26 demonstrated inhibitory effects on NO production,with IC50 values of 3.65,5.48,3.29,6.91,3.12,and 5.67μmol·L^(−1),respectively.Mechanistic studies revealed that compound 1 inhibited IκB kinaseβ(IKKβ)phosphorylation,thereby blocking nuclear factorκB(NF-κB)nuclear translocation,and activated the kelch-like ECH-associated protein 1(Keap1)/nuclear factor erythroid 2-related factor 2(Nrf2)signal pathway,leading to decreased expression of NADPH oxidase 2(NOX-2),inducible nitric oxide synthase(iNOS),tumor necrosis factorα(TNF-α),interleukin-6(IL-6),monocyte chemotactic protein-1(MCP-1),IL-1β,and IL-1αand increased expression of NAD(P)H:quinone oxidoreductase 1(NQO-1)and heme oxygenase-1(HO-1),thus exhibiting anti-inflammatory effects in vitro.These results indicate that dimeric sesquiterpenoids may serve as promising candidates for anti-inflammatory drug development.
文摘以欧亚旋覆花(Inula britannica L.)无菌苗为材料,研究了不同浓度调吡脲(CPPU)对其叶片、根愈伤组织诱导的影响。结果显示,CPPU的浓度为0.10 mg/L时,叶片的愈伤组织诱导率为82.9%,且诱导出的愈伤组织生长好。CPPU的浓度为0.50 mg/L时,根的愈伤组织诱导率最高,达100.0%,愈伤组织生长最好,呈浅黄绿色,部分带有红色斑点,质地疏松,具光泽。
文摘Three new anthranilic acid derivatives, N-heneicosanoylanthranilic acid （1b）, N-tricosanoylanthranilic acid （1d）, N-tetracosanoylanthranilic acid （1e）, and two known N-arachidylanthranilic acid （1a） and N-docosanoylanthranilic acid （1c） were isolated from the aerial parts of lnula japonica Thunb. Their structures were established by spectroscopic and chemical methods.
文摘Two new sesquiterpenes were isolated from the aerial parts of Inula japonica. Their structures were elucidated as 1-hydroxy-8-acetoxycostic acid methyl ester and 1-hydroxy-8-acetoxyisocostic acid methyl ester by spectral methods.
基金Supported by the Science and Technology Development Program of Jilin Province,China(No.20070917-01)the Technology Development Project of Jilin Province,China(No.201205020)
文摘The polysaccharides extracted from Inula britannica flower（IBP） exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography（HPLC） and infrared spectroscopy（IR） methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1：1：1.4：2.7：14.6：6.3：7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide（BCG-LPS）,not only the blood alanine aminotransferase（ALT） and aspartate aminotransferase（AST） levels decreased significantly（P0.05）,but aslo the liver tissue malondialdehyde（MDA） level decreased significantly（P0.01）.The superoxide dismutase（SOD） level increased significantly（P0.01）.IBP shows significant anti-liver injury and antioxidation activity.
基金supported by University Sidi Mohamed Ben Abdellah,Fes,Morocco
文摘Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.
文摘The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.
基金Financial support from the National Science and Technology Major Project"Key New Drug Creation and Manufacturing Program"(Nos.2012ZX09301001-001,2015ZX09103002)the National Natural Science Funds of China(Nos.81302657,81573305,81473112)+3 种基金Youth Innovation Promotion Association CAS,the Ministry of Science and Technology(No.2010DFA30980)the Chinese Academy of Sciences(No.KSZD-EW-Z-004-01)the Shanghai Commission of Science and Technology(No.11DZ1970700,12JC1410300)the Research Fund of University of Macao(MYRG2014-00020-ICMS-QRCM and MYRG2015-00153ICMS-QRCM)
文摘Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC50 values ranging from 2.1 to 36.3 μM.
基金supported by the Scientific and Technological Key Project of Henan Province, China (082102350006 and 102102310242)the College Young Teachers Projects of Henan Province, China (2010GGJS046)
文摘The aim of this study was to determine the extraction technique of supercritical fluid carbon dioxide（SF-CO 2） for the essential oil from Inula britannica flowers and its antifungal activities against plant pathogenic fungi for its potential application as botanical fungicide.The effects of factors,including extraction temperature,extraction pressure,SF-CO 2 flow rate,flower powder size,and time on the essential oil yield were studied using the single factor experiment.An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield.Adopting the optimum conditions,the maximum yield reached 10.01% at 40°C temperature,30 MPa pressure,60 mesh flower powder size,20 L h-1SF-CO 2 flow rate,and 90 min extraction time.The antifungal activities of I.britannica essential oil using the SF-CO 2 against the most important plant pathogenic fungi were also examined through in vitro and in vivo tests.Sixteen plant pathogenic fungi were inhibited to varying degrees at 1 mg mL-1concentration of the essential oil.The mycelial growth of Gaeumannomyces graminis var.tritici was completely inhibited.The radial growths of Phytophthora capsici and Fusarium monilifome were also inhibited by 83.76 and 64.69%,respectively.In addition,the essential oil can inhibit the spore germination of Fusarium oxysporum f.sp.vasinfectum,Phytophthora capsici,Colletotrichum orbiculare,and Pyricularia grisea,and the corresponding inhibition rates were 98.26,96.54,87.89,and 87.35% respectively.The present study has demonstrated that the essential oil of I.britannica flowers extracted through the SF-CO 2 technique is one potential and promising antifungal agent that can be used as botanical fungicide to protect crops.
文摘Experimental assessments were made on the anti-HSV-Ⅱ action of 500 herbs by determinations of thevirus inhibition logarithm(VIL).13 highly effectiveherbs (VIL■4.00)were screened cut,providing a ra-tional basis for clinical therapy.Among these effec-tive herbs,10 were aqueous extracts of Artemisiaanomala,Centella asiatica,Epimedium Sagittatum,Hibiscus mutabills,Hosta plantaginea,Hypericumjaponicum,Inula japonica.Mosla punctata,Rhodo-dendron simsii,and Rhus chinenses,while 3 werealcohol extracts of Epimedium Sagittatum,Hypericumjaponicum,and Mosla punctata.
基金supported by the Agriculture Bio-industry Technology Development Program,Ministry of Agriculture,Food and Rural Affairs of Korea(No.314020-2)the Ministry for Food,Agriculture,Forestry and Fisheries of Korea(No.614102-2)the National Research Foundation of Korea(No.2009-0093824)
文摘The inhibitory effects of Lactobacillus plantarum-fermented and non-fermented Inula britannica extracts on the tyrosinase activity were comparatively investigated to examine whether and how they improve the whitening activity, and the contents of total flavonoids and polyphenolics as bioactive compounds were determined. The skin whitening activity using in vitro or ex vivo tyrosinase and L-3,4-dihydroxyphenylalanine(L-DOPA) staining was examined. The total flavonoid content(TFC) was increased by 13.4% after 72h-fermentation. The viabilities of the B16F10 cells treated with the fermented and non-fermented control extracts were 100.26% and 92.15% at 500μg/ml, respectively. In addition, the inhibition of tyrosinase activity was increased by the fermented samples from 29.33% to 41.74% following fermentation for up to 72h. The tyrosinase activity of the untreated control group was increased to 145.69% in B16F10 cells. The results showed that I. britannica fermented by L. plantarum dose-dependently inhibited tyrosinase activity, which was stimulated by α-melanocyte stimulating hormone. These results suggest that lactic fermented I. britannica extracts can be used as effective skin-whitening materials.
基金Supported by a Grant from the Bursa Uludag University Research Foundation(Isolation of Phenolic Materials from Some Natural Specimens and Investigation of Their Effects on DNA Oxidative DamageNo.OUAP(F)-2013/13)。
文摘OBJECTIVE:To investigate the efficacy of methanol-water extract of Inula helenium root against oxidative DNA damage.METHODS:Antioxidant properties of methanol-water extract of Inula helenium root were revealed by 2,2'-azinobis(3-ethylbenzothiazoline-6-suphonic acid)di-ammonium salt(ABTS),Folin-Ciocalteu and high-performance liquid chromatography(HPLC)methods.The effect against oxidative DNA base damage was determined analyzing oxidative DNA base damage products by GC-MS/MS.RESULTS:The methanol-water extract of Inula helenium root showed good antioxidant capacity[32.20 mg trolox equivalents(TE)/g dried weight(dw)by ABTS method,54.53 mg TE/g dw by chromium reducing antioxidant capacity method]and total phenolic content[29.83 mg gallic acid equivalent(GAE)/g dw].The chlorogenic acid,ferulic acid,rosmarinic acid and caffeic acid were determined in the extract by HPLC.The chlorogenic acid,ferulic acid and Inula helenium root extract were revealed to inhibit the oxidative DNA base damage by GC-MS/MS.CONCLUSION:The studies showed a crucial decrease in the amount of the DNA base damage products when antioxidants were used.The results showed that ferulic acid has better inhibition than chlorogenic acid for DNA oxidation.The methanol-water extract of Inula helenium root was determined to inhibit the oxidative DNA damage.
