Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as ma...Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as main active constituents in IH and IR, exhibit prominent anti-inflammation effect.Objective: To develop a holistic method based on chemical characteristic and anti-inflammation effect for systematically evaluating the quality of twenty-seven TMX samples(including 18 IH samples and 9 IR samples) from different origins.Methods: HPLC fingerprints data of AL(Aucklandia lappa Decne.) whose dried root was similar with HR was added for classification analysis. The HPLC fingerprints of twenty-seven TMX samples and four AL samples were evaluated using hierarchical clustering analysis(HCA) and principle component analysis(PCA). The spectrum-efficacy model between HPLC fingerprints and anti-inflammatory activities was investigated by principal component regression(PCR) and partial least squares(PLS).Results: All samples were successfully divided into three main clusters and peaks 7, 9, 11, 22, 24 and 26 had a primary contribution to classify these medicinal herbs. The results were in accord with the appraisal results of herbs. The spectrum-efficacy relationship results indicated that citric acid, quinic acid, caffeic acid-β-D-glucopyranoside, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoyl quinic acid, tianshic acid and 3β-Hydroxypterondontic acid had main contribution to anti-inflammatory activities.Conclusion: This comprehensive strategy was successfully used for identification of IH, IR and AL, which provided a reliable and adequate theoretical basis for the bioactivity relevant quality standards and studying the material basis of anti-inflammatory effect of TMX.展开更多
本试验采用田间单因素随机区组设计,研究了加施破缕石坡缕石对藏木香产量、品质及土壤团粒结构的影响。结果表明：坡缕石用量750 kg hm-2~3000 kg hm-2时下,均能提高匙藏木香的产量和品质,3000 kg hm-2时产量最高,根径最粗、主根最长、...本试验采用田间单因素随机区组设计,研究了加施破缕石坡缕石对藏木香产量、品质及土壤团粒结构的影响。结果表明：坡缕石用量750 kg hm-2~3000 kg hm-2时下,均能提高匙藏木香的产量和品质,3000 kg hm-2时产量最高,根径最粗、主根最长、植株最高,且较对照极显著增产29.43%（P〈0.01）;2250 kg hm-2多糖含量最高,品质最优;坡缕石能显著增加直径〉0.25mm和0.25～0.5 mm团聚体总数。这一研究结果在藏木香栽培中科学合理的将N、P、K肥料与破缕石配施是提供了科学依据。展开更多
基金the Project of Science and Technology Agency of Gansu(1208RTZA211)Science and Technology Agency of Lanzhou(2013-4-75)the item of scientific and technological researches from Gansu province administration bureau of traditional Chinese medicine(GZK-2014-13)
文摘Background: The dried roots of Inula helenium L.(IH) and Inula racemosa Hook f.(IR) are used commonly as folk medicine under the name of "tumuxiang(TMX)". Phenolic acid compounds and their derivatives, as main active constituents in IH and IR, exhibit prominent anti-inflammation effect.Objective: To develop a holistic method based on chemical characteristic and anti-inflammation effect for systematically evaluating the quality of twenty-seven TMX samples(including 18 IH samples and 9 IR samples) from different origins.Methods: HPLC fingerprints data of AL(Aucklandia lappa Decne.) whose dried root was similar with HR was added for classification analysis. The HPLC fingerprints of twenty-seven TMX samples and four AL samples were evaluated using hierarchical clustering analysis(HCA) and principle component analysis(PCA). The spectrum-efficacy model between HPLC fingerprints and anti-inflammatory activities was investigated by principal component regression(PCR) and partial least squares(PLS).Results: All samples were successfully divided into three main clusters and peaks 7, 9, 11, 22, 24 and 26 had a primary contribution to classify these medicinal herbs. The results were in accord with the appraisal results of herbs. The spectrum-efficacy relationship results indicated that citric acid, quinic acid, caffeic acid-β-D-glucopyranoside, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoyl quinic acid, tianshic acid and 3β-Hydroxypterondontic acid had main contribution to anti-inflammatory activities.Conclusion: This comprehensive strategy was successfully used for identification of IH, IR and AL, which provided a reliable and adequate theoretical basis for the bioactivity relevant quality standards and studying the material basis of anti-inflammatory effect of TMX.
文摘本试验采用田间单因素随机区组设计,研究了加施破缕石坡缕石对藏木香产量、品质及土壤团粒结构的影响。结果表明：坡缕石用量750 kg hm-2~3000 kg hm-2时下,均能提高匙藏木香的产量和品质,3000 kg hm-2时产量最高,根径最粗、主根最长、植株最高,且较对照极显著增产29.43%（P〈0.01）;2250 kg hm-2多糖含量最高,品质最优;坡缕石能显著增加直径〉0.25mm和0.25～0.5 mm团聚体总数。这一研究结果在藏木香栽培中科学合理的将N、P、K肥料与破缕石配施是提供了科学依据。
