Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of...Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC50 values ranging from 2.1 to 36.3 μM.展开更多
目的对旋覆花属8种药用植物进行ITS2序列分析，为其分子鉴定提供依据。方法对4种旋覆花属药用植物的8个样本ITS2序列进行PCR扩增和测序，同时从GenBank下载13个样本。用MEGA5．0计算其种间、种内的K2P距离，及各序列变异位点并进行聚类...目的对旋覆花属8种药用植物进行ITS2序列分析，为其分子鉴定提供依据。方法对4种旋覆花属药用植物的8个样本ITS2序列进行PCR扩增和测序，同时从GenBank下载13个样本。用MEGA5．0计算其种间、种内的K2P距离，及各序列变异位点并进行聚类分析。结果旋覆花属8种药用植物ITS2的长度为210-212bp，变异位点为60个；旋覆花药材2种不同来源药用植物有3个变异位点，与其他同属6种植物有13-43个变异位点；构建的系统发育树显示旋覆花药材的不同基原样本各聚为一支，能很好与其他6种植物区分；旋覆花Inula japonica、欧亚旋覆花I.britannica、总状土木香I.racemosa、土木香I.helenium4个物种的遗传距离值远小于其与羊耳菊I.cappa、赤茎羊耳菊I.rubricaulis、显脉旋覆花I.nervosa、泽兰羊耳菊I.eupatoerioides4个物种的遗传距离值。结论ITS2序列能够为旋覆花属8种药用植物的鉴定和Flora of China对羊耳菊、赤茎羊耳菊、显脉旋覆花、泽兰羊耳菊分类修订提供一定的分子证据。展开更多
基金Financial support from the National Science and Technology Major Project"Key New Drug Creation and Manufacturing Program"(Nos.2012ZX09301001-001,2015ZX09103002)the National Natural Science Funds of China(Nos.81302657,81573305,81473112)+3 种基金Youth Innovation Promotion Association CAS,the Ministry of Science and Technology(No.2010DFA30980)the Chinese Academy of Sciences(No.KSZD-EW-Z-004-01)the Shanghai Commission of Science and Technology(No.11DZ1970700,12JC1410300)the Research Fund of University of Macao(MYRG2014-00020-ICMS-QRCM and MYRG2015-00153ICMS-QRCM)
文摘Phytochemical investigation on the whole plant of Inula cappa led to the isolation of two new germacrane-type sesquiterpene lactones,ineupatolides D and E（1 and 2）,together with three known analogs.The structures of the new compounds were established by extensive analysis of 1Dand 2DNMR spectra,as well as MS data.Their absolute configurations were determined by CD spectra.All compounds showed moderate inhibitory effects on A431,A549,BGC-823,HL-60,HT-29,and MCF-7 cancer cell lines with IC50 values ranging from 2.1 to 36.3 μM.
文摘目的对旋覆花属8种药用植物进行ITS2序列分析，为其分子鉴定提供依据。方法对4种旋覆花属药用植物的8个样本ITS2序列进行PCR扩增和测序，同时从GenBank下载13个样本。用MEGA5．0计算其种间、种内的K2P距离，及各序列变异位点并进行聚类分析。结果旋覆花属8种药用植物ITS2的长度为210-212bp，变异位点为60个；旋覆花药材2种不同来源药用植物有3个变异位点，与其他同属6种植物有13-43个变异位点；构建的系统发育树显示旋覆花药材的不同基原样本各聚为一支，能很好与其他6种植物区分；旋覆花Inula japonica、欧亚旋覆花I.britannica、总状土木香I.racemosa、土木香I.helenium4个物种的遗传距离值远小于其与羊耳菊I.cappa、赤茎羊耳菊I.rubricaulis、显脉旋覆花I.nervosa、泽兰羊耳菊I.eupatoerioides4个物种的遗传距离值。结论ITS2序列能够为旋覆花属8种药用植物的鉴定和Flora of China对羊耳菊、赤茎羊耳菊、显脉旋覆花、泽兰羊耳菊分类修订提供一定的分子证据。
