Probiotics are natural systems bridging synthetic biology,physical biotechnology,and immunology,initiating innate and adaptive anti-tumor immune activity.We previously constructed an all-inone engineered food-grade pr...Probiotics are natural systems bridging synthetic biology,physical biotechnology,and immunology,initiating innate and adaptive anti-tumor immune activity.We previously constructed an all-inone engineered food-grade probiotic Lactococcus lactis(FOLactis)which could boost the crosstalk among different immune cells such as dendritic cells(DCs),natural killer cells,and T cells.Herein,considering the limited clinical efficacy of naked personalized neoantigen peptide vaccines,we decorate FOLactis with tumor antigens by employing a Plug-and-Display system comprising membrane-inserted peptides.Intranodal injection of FOLactis coated with neoantigen peptides(Ag-FOLactis)induces robust DCs presentation and neoantigen-specific cellular immunity.Notably,Ag-FOLactis not only triggers a 45-fold rise in the quantity of locally reactive neoantigen-specific T cells but also induces epitope spreading in both subcutaneous and metastatic tumor-bearing models,leading to potent inhibition of tumor growth.These findings imply that Ag-FOLactis represents a powerful platform to rapidly and easily display antigens,facilitating the development of a bio-activated platform for personalized therapy.展开更多
Intranodal palisaded myofibroblastoma （IPM）, a benign, rare primary mesenchymal neoplasm, located in the inguinal lymph nodes, was described as “ palisaded myofibroblastoma” by Weiss et al, and “intranodal hemorr...Intranodal palisaded myofibroblastoma （IPM）, a benign, rare primary mesenchymal neoplasm, located in the inguinal lymph nodes, was described as “ palisaded myofibroblastoma” by Weiss et al, and “intranodal hemorrhagic spindlecell tumor with amianthoid fibers” by Suster and Rosai. Similar neoplasms have been previously reported as intranodal schwannoma. The age of the patients ranged between 19-70 years, and more men are affected than women.展开更多
基金supported by the National Natural Science Foundation of China(81930080 and 82272811)the Fund for Distinguished Young Scholars of Jiangsu Province(BK20230001,China)the Key Project Foundation of Nanjing for the Development of Medical Technology(ID:ZKX20024,China).
文摘Probiotics are natural systems bridging synthetic biology,physical biotechnology,and immunology,initiating innate and adaptive anti-tumor immune activity.We previously constructed an all-inone engineered food-grade probiotic Lactococcus lactis(FOLactis)which could boost the crosstalk among different immune cells such as dendritic cells(DCs),natural killer cells,and T cells.Herein,considering the limited clinical efficacy of naked personalized neoantigen peptide vaccines,we decorate FOLactis with tumor antigens by employing a Plug-and-Display system comprising membrane-inserted peptides.Intranodal injection of FOLactis coated with neoantigen peptides(Ag-FOLactis)induces robust DCs presentation and neoantigen-specific cellular immunity.Notably,Ag-FOLactis not only triggers a 45-fold rise in the quantity of locally reactive neoantigen-specific T cells but also induces epitope spreading in both subcutaneous and metastatic tumor-bearing models,leading to potent inhibition of tumor growth.These findings imply that Ag-FOLactis represents a powerful platform to rapidly and easily display antigens,facilitating the development of a bio-activated platform for personalized therapy.
文摘Intranodal palisaded myofibroblastoma （IPM）, a benign, rare primary mesenchymal neoplasm, located in the inguinal lymph nodes, was described as “ palisaded myofibroblastoma” by Weiss et al, and “intranodal hemorrhagic spindlecell tumor with amianthoid fibers” by Suster and Rosai. Similar neoplasms have been previously reported as intranodal schwannoma. The age of the patients ranged between 19-70 years, and more men are affected than women.
