Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B(1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. I...Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B(1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the ^(15)N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.展开更多
基金supported by the National Natural Science Foundation of China (Nos. U1702285,21708045 and21502203)the Natural Science Foundation of Yunnan Province(Nos. 2019FJ007,2019FA034 and 2018HC012)+1 种基金the Key Research Program of Frontier Sciences and the Strategic Priority Research Program,CAS (Nos. QYZDB-SSW-SMC051 and XDB27020205)the Syngenta Postgraduate Studentship Awarded to WANG Yong-Jiang (2019-2020)。
文摘Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B(1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the ^(15)N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.
