This paper explores the association between intestinal microecology and digestive health and disease recovery in children with pneumonia.Intestinal microecological imbalance is common in children with pneumonia,which ...This paper explores the association between intestinal microecology and digestive health and disease recovery in children with pneumonia.Intestinal microecological imbalance is common in children with pneumonia,which is closely associated with digestive health and disease recovery.Intestinal microecological imbalance may affect digestive enzyme activity,intestinal mucosal barrier function,and nutrient absorption,which in turn affects digestive health.In addition,intestinal microecological imbalances may be associated with immune regulation,inflammatory responses,and pathogen suppression,affecting disease recovery.Strategies to regulate intestinal microecology include probiotic supplementation,dietary modification,and pharmacological treatment.Currently,the study of intestinal microecology in children with pneumonia faces challenges,and there is a need for improved research methods,individualized treatment strategies,and the development of novel probiotics.In conclusion,the intestinal microecology of children with pneumonia is closely related to digestive health and disease recovery,and the regulation of intestinal microecology is of great significance to the treatment of children with pneumonia.Furthermore,future research should further explore the application of the microecology of the intestinal microecology in the treatment of children with pneumonia.展开更多
BACKGROUND Sepsis exacerbates intestinal microecological disorders leading to poor prognosis.Proper modalities of nutritional support can improve nutrition,immunity,and intestinal microecology.AIM To identify the opti...BACKGROUND Sepsis exacerbates intestinal microecological disorders leading to poor prognosis.Proper modalities of nutritional support can improve nutrition,immunity,and intestinal microecology.AIM To identify the optimal modality of early nutritional support for patients with sepsis from the perspective of intestinal microecology.METHODS Thirty patients with sepsis admitted to the intensive care unit of the General Hospital of Ningxia Medical University,China,between 2019 and 2021 with indications for nutritional support,were randomly assigned to one of three different modalities of nutritional support for a total of 5 d:Total enteral nutrition(TEN group),total parenteral nutrition(TPN group),and supplemental parenteral nutrition(SPN group).Blood and stool specimens were collected before and after nutritional support,and changes in gut microbiota,short-chain fatty acids(SCFAs),and immune and nutritional indicators were detected and compared among the three groups.RESULTS In comparison with before nutritional support,the three groups after nutritional support presented:(1)Differences in the gut bacteria(Enterococcus increased in the TEN group,Campylobacter decreased in the TPN group,and Dialister decreased in the SPN group;all P<0.05);(2)different trends in SCFAs(the TEN group showed improvement except for Caproic acid,the TPN group showed improvement only for acetic and propionic acid,and the SPN group showed a decreasing trend);(3)significant improvement of the nutritional and immunological indicators in the TEN and SPN groups,while only immunoglobulin G improved in the TPN group(all P<0.05);and(4)a significant correlation was found between the gut bacteria,SCFAs,and nutritional and immunological indicators(all P<0.05).CONCLUSION TEN is recommended as the preferred mode of early nutritional support in sepsis based on clinical nutritional and immunological indicators,as well as changes in intestinal microecology.展开更多
Inflammatory bowel disease(IBD)is a disorder of the immune system and intestinal microecosystem caused by environmental factors in genetically susceptible people.Paneth cells(PCs)play a central role in IBD pathogenesi...Inflammatory bowel disease(IBD)is a disorder of the immune system and intestinal microecosystem caused by environmental factors in genetically susceptible people.Paneth cells(PCs)play a central role in IBD pathogenesis,especially in Crohn's disease development,and their morphology,number and function are regulated by susceptibility genes.In the intestine,PCs participate in the formation of the stem cell microenvironment by secreting antibacterial particles and play a role in helping maintain the intestinal microecology and intestinal mucosal homeostasis.Moreover,PC proliferation and maturation depend on symbiotic flora in the intestine.This paper describes the interactions among susceptibility genes,PCs and intestinal microecology and their effects on IBD occurrence and development.展开更多
Rifaximin in the Treatment of Gastroesophageal Reflux Disease (GERD) is a common clinical disorder, the most common symptom of which is a burning sensation behind the breastbone (heartburn) or reflux of stomach conten...Rifaximin in the Treatment of Gastroesophageal Reflux Disease (GERD) is a common clinical disorder, the most common symptom of which is a burning sensation behind the breastbone (heartburn) or reflux of stomach contents into the upper pharynx (acid reflux). The prevalence in China is increasing year by year, which can affect the quality of life of patients and also increase the economic burden on families and society. The pathogenesis of GERD is still unclear, and some studies suggest that intestinal microecology may be closely related to the development of GERD. Rifaximin is not readily absorbed orally and acts locally in the intestine, so it has mild adverse effects and good safety, and can be used to treat gastrointestinal diseases such as irritable bowel syndrome, traveler’s diarrhea, small intestinal bacterial overgrowth, diverticulosis, inflammatory bowel disease and hepatic encephalopathy. Therefore, this paper focuses on intestinal microecology as a possible pathogenesis of GERD and further explores the feasibility of rifaximin for the treatment of GERD.展开更多
Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestina...Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestinal flora of MHE,as well as the target of action of Rhubarb Decoction.Methods Totally 45 rats were randomly divided into the normal control group(NC),the MHE model group(MHE),and the treatment group(MHEY)for the 30 g of Mume fructus and 30 g of vinegar-made Rhubarb,15 in each group.Rat in the MHE and HE groups were induced by intraperitoneal injection of tetrachloromethane(CCl,)and thioacetamide(TAA).After successful modeling,10 rats in each group were selected for comparative analysis.The water maze experiment was used to evaluate the learning and memory abilities of rats in each group before and after treatment,and biochemical indices were analyzed.16SrDNA sequencing technology was used to analyze the changes in the number,structure,and abundance of the intestinal flora of rats in each group.Results Compared with the NC group,the water maze test search time was prolonged,the levels of aspartate transaminase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),total bile acid(TBA),and blood ammonia increased,ALB decreased(P<0.05)in the MHEgroup.Compared with the MHE group,the water maze test search time was shortened,the levels of AST,ALT,TBIL,TBA,and blood ammonia decreased,ALB increased(P<0.05)in the MHEYgroup.16SrDNA technology revealed that,comparedwith group NC,Firmicutes andCyanobacteria were down-regulated,Bacteroidetes and Actinobacteria were up-regulated in MHE group and MHEY group.While the good bacteria proliferated in group MHEY,the detrimental bacteria rose dramaticallyin groupNMHE.AAdditionally,actinomyces in group MHEY had a declining tendency,and lactobacillus emerged as the dominating species following treatment of Rhubarb Decoction.The Clostridium genus,Clostridia order,Ruminococcaceae,Verrucomycetes family,Trichospiridae family,etc.were among the microbial markers of the MHEYYgroup.Conclusion Rhubarb Decoction can reduce the number of harmful intestinal flora in rats with mild hepatic encephalopathy.MHE can be recovered by acidifying the intestinal tract and restoring intestinal flora balance using the"Tongfu-opening method"of Chinese medicine.展开更多
In this editorial,we discuss the findings reported by Wang et al in the latest issue of the World Journal of Gastrointestinal Oncology.Various research methodologies,including microbiome analysis,assert that the Tzu-C...In this editorial,we discuss the findings reported by Wang et al in the latest issue of the World Journal of Gastrointestinal Oncology.Various research methodologies,including microbiome analysis,assert that the Tzu-Chi Cancer-Antagonizing and Life-Protecting II Decoction of Chinese herbal compounds mitigates inflammatory responses by inhibiting the NF-κB signaling pathway.This action helps maintain the dynamic equilibrium of the intestinal microecology and lessens chemotherapy-induced gastrointestinal damage.The efficacy of these compounds is intimately linked to the composition of intestinal microbes.These compounds regulate intestinal microecology by virtue of their specific compatibility and effectiveness,thereby enhancing the overall therapeutic outcomes of cancer chemotherapy.Nonetheless,the exact mechanisms underlying these effects warrant further investigation.Multi-omics technologies offer a systematic approach to elucidate the mechanisms and effectiveness of Chinese herbal compounds in vivo.This manuscript reviews the application of multi-omics technologies to Chinese herbal compounds and explores their potential role in modulating the gastrointestinal microenvironment following cancer chemotherapy,thus providing a theoretical foundation for their continued use in adjunct cancer treatment.展开更多
Non-alcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases worldwide,causing serious economic and medical burdens.Currently,Chinese medicine(CM)has become an important means in tr...Non-alcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases worldwide,causing serious economic and medical burdens.Currently,Chinese medicine(CM)has become an important means in treating NAFLD in China.Intestinal microecology(IM)is an important part of the internal environment in the human body and is involved in the occurrence and development of NAFLD.In this paper,the authors systematically discuss the significance of IM in the pathogenesis of NAFLD and the current status of research on the CM treatment of NAFLD via IM regulation.In combination with our own research practice,we propose that IM is an important target for the treatment of NAFLD with CM and formulate plans for future research to target limitations existing in current studies.展开更多
BACKGROUND The gut microbiota is strongly associated with radiation-induced gut damage.This study aimed to assess the effectiveness and safety of intestinal microecological transplantation for treating patients with c...BACKGROUND The gut microbiota is strongly associated with radiation-induced gut damage.This study aimed to assess the effectiveness and safety of intestinal microecological transplantation for treating patients with chronic radiation enteritis.CASE SUMMARY A 64-year-old female with cervical cancer developed abdominal pain,diarrhea,and blood in the stool 1 year after radiotherapy.An electronic colonoscopy was performed to diagnose chronic radiation enteritis.Two courses of intestinal microecological transplantation and full-length 16S rRNA microbiological analysis were performed.The patient experienced short-and long-term relief from symptoms without adverse effects.Whole 16S rRNA sequencing revealed significant differences in the intestinal flora’s composition between patient and healthy donors.Pathogenic bacteria,such as Escherichia fergusonii and Romboutsia timonensis,were more in the patient.Beneficial bacteria such as Faecalibacterium prausnitzii,Fusicatenibacter saccharivorans,Ruminococcus bromii,and Bifidobacterium longum were more in the healthy donors.Intestinal microbiota transplantation resulted in a significant change in the patient's intestinal flora composition.The composition converged with the donor's flora,with an increase in core beneficial intestinal bacteria,such as Eubacterium rectale,and a decrease in pathogenic bacteria.Changes in the intestinal flora corresponded with the patients'alleviating clinical symptoms.CONCLUSION Intestinal microecological transplantation is an effective treatment for relieving the clinical symptoms of chronic radiation enteritis by altering the composition of the intestinal flora.This study provides a new approach for treating patients with chronic radiation enteritis.展开更多
The intestinal microecological disorder is closely related to the occurrence and development of lung cancer and the treatment response.This paper starts with the relationship between the lungintestinal axis,to discuss...The intestinal microecological disorder is closely related to the occurrence and development of lung cancer and the treatment response.This paper starts with the relationship between the lungintestinal axis,to discuss the possibility of TCM regulating the target of intestinal microecological treatment of lung cancer,so as to provide new research ideas for TCM treatment of lung cancer.展开更多
BACKGROUND Jianpi-Huatan-Huoxue-Anshen formula[Tzu-Chi cancer-antagonizing&lifeprotecting II decoction(TCCL)]is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects ...BACKGROUND Jianpi-Huatan-Huoxue-Anshen formula[Tzu-Chi cancer-antagonizing&lifeprotecting II decoction(TCCL)]is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects of chemotherapy in cancer patients and improve their quality of life.However,its effect and mechanism on the intestinal microecology after chemotherapy are not yet clear.AIM To discover the potential mechanisms of TCCL on gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with hepatocellular carcinoma(HCC).METHODS Ninety-six mice were inoculated subcutaneously with HCC cells.One week later,the mice received a large dose of 5-fluorouracil by intraperitoneal injection to establish a HCC chemotherapy model.Thirty-six mice were randomly selected before administration,and feces,ileal tissue,and ileal contents were collected from each mouse.The remaining mice were randomized into normal saline,continuous chemotherapy,Yangzheng Xiaoji capsulestreated,and three TCCL-treated groups.After treatment,feces,tumors,liver,spleen,thymus,stomach,jejunum,ileum,and colon tissues,and ileal contents were collected.Morphological changes,serum levels of IL-1β,IL-6,IL-8,IL-10,IL-22,TNF-α,and TGF-β,intestinal SIgA,and protein and mRNA expression of ZO-1,NF-κB,Occludin,MUC-2,Claudin-1,and IκB-αin colon tissues were documented.The effect of TCCL on the abundance and diversity of intestinal flora was analyzed using 16S rDNA sequencing.RESULTS TCCL treatment improved thymus and spleen weight,thymus and spleen indexes,and body weight,decreased tumor volumes and tumor tissue cell density,and alleviated injury to gastric,ileal,and colonic mucosal tissues.Among proteins and genes associated with inflammation,IL-10,TGF-β,SIgA,ZO-1,MUC-2,and Occludin were upregulated,whereas NF-κB,IL-1β,IL-6,TNF-α,IL-22,IL-8,and IκB-αwere downregulated.Additionally,TCCL increased the proportions of fecal Actinobacteria,AF12,Adlercreutzia,Clostridium,Coriobacteriaceae,and Paraprevotella in the intermediate stage of treatment,decreased the proportions of Mucipirillum,Odoribacter,RF32,YS2,and Rikenellaceae but increased the proportions of p_Deferribacteres and Lactobacillus at the end of treatment.Studies on ileal mucosal microbiota showed similar findings.Moreover,TCCL improved community richness,evenness,and the diversity of fecal and ileal mucosal flora.CONCLUSION TCCL relieves pathological changes in tumor tissue and chemotherapy-induced gastrointestinal injury,potentially by reducing the release of pro-inflammatory factors to repair the gastrointestinal mucosa,enhancing intestinal barrier function,and maintaining gastrointestinal microecological balance.Hence,TCCL is a very effective adjuvant to chemotherapy.展开更多
The incidence of ulcerative colitis is increasing year by year,yet the pathogenesis is still not clear.Many scholars have studied the genetic factors,environmental factors,intestinal microecological imbalance,intestin...The incidence of ulcerative colitis is increasing year by year,yet the pathogenesis is still not clear.Many scholars have studied the genetic factors,environmental factors,intestinal microecological imbalance,intestinal mucosal barrier disorder,abnormal immune response and mitochondrial diseases,and abundant achievements have been made.In order to further understand the possible pathogenesis of ulcerative colitis,this paper reviews its research progress,in order to better guide clinical medication,and provide new ideas for further study of its pathogenesis.展开更多
Objective: to investigate the effects of long-life diet combined with bifidobacteria triple viable enteric-soluble capsules on glucose and lipid metabolism in patients with type 2 diabetes mellitus. Methods: in a doub...Objective: to investigate the effects of long-life diet combined with bifidobacteria triple viable enteric-soluble capsules on glucose and lipid metabolism in patients with type 2 diabetes mellitus. Methods: in a double-cycle, crossover design, subjects were randomly divided into experimental group (macrobiotic diet combined with bifidobacterium triad live enteric-soluble capsule) and control group (diabetic dietary guidance). Each group lasted for 4 weeks, with a 4-week washout interval. Baseline and after the intervention were detected and compared two groups of participants' body weight (BW), body mass index (BMI), waist circumference (WC), hip circumference (HC), fasting plasma glucose (FPG), postprandial 2 hours blood glucose (2 HPBG), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL - C) and low density lipoprotein cholesterol ( HDL-C). Results: compared with the control group, the decrease of FPG, 2hPBG, TG, BW and BMI in the experimental group was statistically significant (P < 0.05). There was no statistical significance in the decrease of TC, HDL-C, LDL-C, WC and HC between the two groups (P > 0.05).Conclusion: the long-life diet combined with bifidobacterium triple viable enteric capsule can improve the blood glucose level, some lipid metabolism indicators and anthropometric indicators of type 2 diabetes patients.展开更多
Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: b...Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.展开更多
Background and Objectives:Observational studies have shown that energy restriction could be beneficial for controlling bodyweight in patients with polycystic ovary syndrome(PCOS).We aim to compare the effects of a hig...Background and Objectives:Observational studies have shown that energy restriction could be beneficial for controlling bodyweight in patients with polycystic ovary syndrome(PCOS).We aim to compare the effects of a high-protein diet(HPD),a high-protein and high-dietary fiber diet(HPHFD),and a calorie-restricted diet(CRD)on metabolic health and gut microbiota in overweight/obese PCOS patients.Methods and Study Design:We will enroll a total of 90 overweight/obese PCOS patients into this eight-week open-label randomised controlled trial.Participants will be randomly assigned to three groups:CRD group(energy coefficient 20 kcal/kg.day,water≥1500 mL,0.8-1.2 g/kg protein,carbohydrate energize 55-60%,and fat energize 25-30%),HDP group(energy coefficient 20 kcal/kg.day,water≥1500 mL,and 1.5-2.0 g/kg protein)and HPHFD group(based on the high protein diet with 15 g more dietary fiber supplement).The primary outcome is body weight,body fat percentage,and lean body mass.The secondary outcomes will include changes in blood lipids,inflammation,glucose tolerance,blood pressure,and gut microbiota compositions.Between-group differences in adiposity measurements at baseline will be compared using one-way analysis of variance(ANOVA)or Kruskal-Wallis test when appropriate.Within-group difference after 8-week intervention will be compared using paired t-test or Wilcoxon signed rank test.Between-group differences in adiposity measurements after 8-week diet intervention will be compared using linear mixed model and ANCOVA.The gut microbiota will be analyzed using 16S amplicon sequencing and the sequencing data will be analyzed using the standardized QIIME2 piperline.展开更多
基金Shandong Province Traditional Chinese Medicine Science and Technology Project"Efficacy Evaluation of Acupoint Application Synergy Model Intervention in Bronchoscopic Treatment of Severe Mycoplasma Pneumonia in Children"(Project No.2020M177)。
文摘This paper explores the association between intestinal microecology and digestive health and disease recovery in children with pneumonia.Intestinal microecological imbalance is common in children with pneumonia,which is closely associated with digestive health and disease recovery.Intestinal microecological imbalance may affect digestive enzyme activity,intestinal mucosal barrier function,and nutrient absorption,which in turn affects digestive health.In addition,intestinal microecological imbalances may be associated with immune regulation,inflammatory responses,and pathogen suppression,affecting disease recovery.Strategies to regulate intestinal microecology include probiotic supplementation,dietary modification,and pharmacological treatment.Currently,the study of intestinal microecology in children with pneumonia faces challenges,and there is a need for improved research methods,individualized treatment strategies,and the development of novel probiotics.In conclusion,the intestinal microecology of children with pneumonia is closely related to digestive health and disease recovery,and the regulation of intestinal microecology is of great significance to the treatment of children with pneumonia.Furthermore,future research should further explore the application of the microecology of the intestinal microecology in the treatment of children with pneumonia.
基金Supported by Ningxia Natural Science Foundation Project,No.2022AAC03488the National Key Research and Development Program of China,No.2016YFD0400605.
文摘BACKGROUND Sepsis exacerbates intestinal microecological disorders leading to poor prognosis.Proper modalities of nutritional support can improve nutrition,immunity,and intestinal microecology.AIM To identify the optimal modality of early nutritional support for patients with sepsis from the perspective of intestinal microecology.METHODS Thirty patients with sepsis admitted to the intensive care unit of the General Hospital of Ningxia Medical University,China,between 2019 and 2021 with indications for nutritional support,were randomly assigned to one of three different modalities of nutritional support for a total of 5 d:Total enteral nutrition(TEN group),total parenteral nutrition(TPN group),and supplemental parenteral nutrition(SPN group).Blood and stool specimens were collected before and after nutritional support,and changes in gut microbiota,short-chain fatty acids(SCFAs),and immune and nutritional indicators were detected and compared among the three groups.RESULTS In comparison with before nutritional support,the three groups after nutritional support presented:(1)Differences in the gut bacteria(Enterococcus increased in the TEN group,Campylobacter decreased in the TPN group,and Dialister decreased in the SPN group;all P<0.05);(2)different trends in SCFAs(the TEN group showed improvement except for Caproic acid,the TPN group showed improvement only for acetic and propionic acid,and the SPN group showed a decreasing trend);(3)significant improvement of the nutritional and immunological indicators in the TEN and SPN groups,while only immunoglobulin G improved in the TPN group(all P<0.05);and(4)a significant correlation was found between the gut bacteria,SCFAs,and nutritional and immunological indicators(all P<0.05).CONCLUSION TEN is recommended as the preferred mode of early nutritional support in sepsis based on clinical nutritional and immunological indicators,as well as changes in intestinal microecology.
文摘Inflammatory bowel disease(IBD)is a disorder of the immune system and intestinal microecosystem caused by environmental factors in genetically susceptible people.Paneth cells(PCs)play a central role in IBD pathogenesis,especially in Crohn's disease development,and their morphology,number and function are regulated by susceptibility genes.In the intestine,PCs participate in the formation of the stem cell microenvironment by secreting antibacterial particles and play a role in helping maintain the intestinal microecology and intestinal mucosal homeostasis.Moreover,PC proliferation and maturation depend on symbiotic flora in the intestine.This paper describes the interactions among susceptibility genes,PCs and intestinal microecology and their effects on IBD occurrence and development.
文摘Rifaximin in the Treatment of Gastroesophageal Reflux Disease (GERD) is a common clinical disorder, the most common symptom of which is a burning sensation behind the breastbone (heartburn) or reflux of stomach contents into the upper pharynx (acid reflux). The prevalence in China is increasing year by year, which can affect the quality of life of patients and also increase the economic burden on families and society. The pathogenesis of GERD is still unclear, and some studies suggest that intestinal microecology may be closely related to the development of GERD. Rifaximin is not readily absorbed orally and acts locally in the intestine, so it has mild adverse effects and good safety, and can be used to treat gastrointestinal diseases such as irritable bowel syndrome, traveler’s diarrhea, small intestinal bacterial overgrowth, diverticulosis, inflammatory bowel disease and hepatic encephalopathy. Therefore, this paper focuses on intestinal microecology as a possible pathogenesis of GERD and further explores the feasibility of rifaximin for the treatment of GERD.
文摘Objective To analyze the changes of intestinal flora of rats with minimal hepatic encephalopathy(MHE)after the treatment of Rhubarb Decoction using 16SrDNA sequencing technology,and to explore the changes of intestinal flora of MHE,as well as the target of action of Rhubarb Decoction.Methods Totally 45 rats were randomly divided into the normal control group(NC),the MHE model group(MHE),and the treatment group(MHEY)for the 30 g of Mume fructus and 30 g of vinegar-made Rhubarb,15 in each group.Rat in the MHE and HE groups were induced by intraperitoneal injection of tetrachloromethane(CCl,)and thioacetamide(TAA).After successful modeling,10 rats in each group were selected for comparative analysis.The water maze experiment was used to evaluate the learning and memory abilities of rats in each group before and after treatment,and biochemical indices were analyzed.16SrDNA sequencing technology was used to analyze the changes in the number,structure,and abundance of the intestinal flora of rats in each group.Results Compared with the NC group,the water maze test search time was prolonged,the levels of aspartate transaminase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),total bile acid(TBA),and blood ammonia increased,ALB decreased(P<0.05)in the MHEgroup.Compared with the MHE group,the water maze test search time was shortened,the levels of AST,ALT,TBIL,TBA,and blood ammonia decreased,ALB increased(P<0.05)in the MHEYgroup.16SrDNA technology revealed that,comparedwith group NC,Firmicutes andCyanobacteria were down-regulated,Bacteroidetes and Actinobacteria were up-regulated in MHE group and MHEY group.While the good bacteria proliferated in group MHEY,the detrimental bacteria rose dramaticallyin groupNMHE.AAdditionally,actinomyces in group MHEY had a declining tendency,and lactobacillus emerged as the dominating species following treatment of Rhubarb Decoction.The Clostridium genus,Clostridia order,Ruminococcaceae,Verrucomycetes family,Trichospiridae family,etc.were among the microbial markers of the MHEYYgroup.Conclusion Rhubarb Decoction can reduce the number of harmful intestinal flora in rats with mild hepatic encephalopathy.MHE can be recovered by acidifying the intestinal tract and restoring intestinal flora balance using the"Tongfu-opening method"of Chinese medicine.
基金Supported by 2023 Government-funded Project of the Outstanding Talents Training Program in Clinical Medicine,No.ZF2023165Key Research and Development Projects of Hebei Province,No.18277731DNatural Science Foundation of Hebei Province,No.H202423105.
文摘In this editorial,we discuss the findings reported by Wang et al in the latest issue of the World Journal of Gastrointestinal Oncology.Various research methodologies,including microbiome analysis,assert that the Tzu-Chi Cancer-Antagonizing and Life-Protecting II Decoction of Chinese herbal compounds mitigates inflammatory responses by inhibiting the NF-κB signaling pathway.This action helps maintain the dynamic equilibrium of the intestinal microecology and lessens chemotherapy-induced gastrointestinal damage.The efficacy of these compounds is intimately linked to the composition of intestinal microbes.These compounds regulate intestinal microecology by virtue of their specific compatibility and effectiveness,thereby enhancing the overall therapeutic outcomes of cancer chemotherapy.Nonetheless,the exact mechanisms underlying these effects warrant further investigation.Multi-omics technologies offer a systematic approach to elucidate the mechanisms and effectiveness of Chinese herbal compounds in vivo.This manuscript reviews the application of multi-omics technologies to Chinese herbal compounds and explores their potential role in modulating the gastrointestinal microenvironment following cancer chemotherapy,thus providing a theoretical foundation for their continued use in adjunct cancer treatment.
基金Supported by National Natural Science Foundation of China(No.81830119,81873109)。
文摘Non-alcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases worldwide,causing serious economic and medical burdens.Currently,Chinese medicine(CM)has become an important means in treating NAFLD in China.Intestinal microecology(IM)is an important part of the internal environment in the human body and is involved in the occurrence and development of NAFLD.In this paper,the authors systematically discuss the significance of IM in the pathogenesis of NAFLD and the current status of research on the CM treatment of NAFLD via IM regulation.In combination with our own research practice,we propose that IM is an important target for the treatment of NAFLD with CM and formulate plans for future research to target limitations existing in current studies.
文摘BACKGROUND The gut microbiota is strongly associated with radiation-induced gut damage.This study aimed to assess the effectiveness and safety of intestinal microecological transplantation for treating patients with chronic radiation enteritis.CASE SUMMARY A 64-year-old female with cervical cancer developed abdominal pain,diarrhea,and blood in the stool 1 year after radiotherapy.An electronic colonoscopy was performed to diagnose chronic radiation enteritis.Two courses of intestinal microecological transplantation and full-length 16S rRNA microbiological analysis were performed.The patient experienced short-and long-term relief from symptoms without adverse effects.Whole 16S rRNA sequencing revealed significant differences in the intestinal flora’s composition between patient and healthy donors.Pathogenic bacteria,such as Escherichia fergusonii and Romboutsia timonensis,were more in the patient.Beneficial bacteria such as Faecalibacterium prausnitzii,Fusicatenibacter saccharivorans,Ruminococcus bromii,and Bifidobacterium longum were more in the healthy donors.Intestinal microbiota transplantation resulted in a significant change in the patient's intestinal flora composition.The composition converged with the donor's flora,with an increase in core beneficial intestinal bacteria,such as Eubacterium rectale,and a decrease in pathogenic bacteria.Changes in the intestinal flora corresponded with the patients'alleviating clinical symptoms.CONCLUSION Intestinal microecological transplantation is an effective treatment for relieving the clinical symptoms of chronic radiation enteritis by altering the composition of the intestinal flora.This study provides a new approach for treating patients with chronic radiation enteritis.
基金Independently selected project of basic scientific research expenses of China Academy of Chinese Medical Sciences(subject No.:zz11-069)
文摘The intestinal microecological disorder is closely related to the occurrence and development of lung cancer and the treatment response.This paper starts with the relationship between the lungintestinal axis,to discuss the possibility of TCM regulating the target of intestinal microecological treatment of lung cancer,so as to provide new research ideas for TCM treatment of lung cancer.
基金Supported by Natural Science Foundation of Xiamen,China,No.3502Z20227171the Young Investigator Research Program of Xiang’an Hospital of Xiamen University,No.XAH23005+2 种基金the Traditional Chinese Medicine Foundation of Xiamen,No.XWZY-2023-0103Natural Science Foundation of Fujian,China,No.2018J01136National Natural Science Foundation of China,No.81202659.
文摘BACKGROUND Jianpi-Huatan-Huoxue-Anshen formula[Tzu-Chi cancer-antagonizing&lifeprotecting II decoction(TCCL)]is a Chinese medical formula that has been clinically shown to reduce the gastrointestinal side effects of chemotherapy in cancer patients and improve their quality of life.However,its effect and mechanism on the intestinal microecology after chemotherapy are not yet clear.AIM To discover the potential mechanisms of TCCL on gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with hepatocellular carcinoma(HCC).METHODS Ninety-six mice were inoculated subcutaneously with HCC cells.One week later,the mice received a large dose of 5-fluorouracil by intraperitoneal injection to establish a HCC chemotherapy model.Thirty-six mice were randomly selected before administration,and feces,ileal tissue,and ileal contents were collected from each mouse.The remaining mice were randomized into normal saline,continuous chemotherapy,Yangzheng Xiaoji capsulestreated,and three TCCL-treated groups.After treatment,feces,tumors,liver,spleen,thymus,stomach,jejunum,ileum,and colon tissues,and ileal contents were collected.Morphological changes,serum levels of IL-1β,IL-6,IL-8,IL-10,IL-22,TNF-α,and TGF-β,intestinal SIgA,and protein and mRNA expression of ZO-1,NF-κB,Occludin,MUC-2,Claudin-1,and IκB-αin colon tissues were documented.The effect of TCCL on the abundance and diversity of intestinal flora was analyzed using 16S rDNA sequencing.RESULTS TCCL treatment improved thymus and spleen weight,thymus and spleen indexes,and body weight,decreased tumor volumes and tumor tissue cell density,and alleviated injury to gastric,ileal,and colonic mucosal tissues.Among proteins and genes associated with inflammation,IL-10,TGF-β,SIgA,ZO-1,MUC-2,and Occludin were upregulated,whereas NF-κB,IL-1β,IL-6,TNF-α,IL-22,IL-8,and IκB-αwere downregulated.Additionally,TCCL increased the proportions of fecal Actinobacteria,AF12,Adlercreutzia,Clostridium,Coriobacteriaceae,and Paraprevotella in the intermediate stage of treatment,decreased the proportions of Mucipirillum,Odoribacter,RF32,YS2,and Rikenellaceae but increased the proportions of p_Deferribacteres and Lactobacillus at the end of treatment.Studies on ileal mucosal microbiota showed similar findings.Moreover,TCCL improved community richness,evenness,and the diversity of fecal and ileal mucosal flora.CONCLUSION TCCL relieves pathological changes in tumor tissue and chemotherapy-induced gastrointestinal injury,potentially by reducing the release of pro-inflammatory factors to repair the gastrointestinal mucosa,enhancing intestinal barrier function,and maintaining gastrointestinal microecological balance.Hence,TCCL is a very effective adjuvant to chemotherapy.
基金supported by National Famous Traditional Chinese Medicine Experts Inheritance Studio Construction Project of State Administration of Traditional Chinese Medicine(National Traditional Chinese Medicine Education Letter[2016]No.42)。
文摘The incidence of ulcerative colitis is increasing year by year,yet the pathogenesis is still not clear.Many scholars have studied the genetic factors,environmental factors,intestinal microecological imbalance,intestinal mucosal barrier disorder,abnormal immune response and mitochondrial diseases,and abundant achievements have been made.In order to further understand the possible pathogenesis of ulcerative colitis,this paper reviews its research progress,in order to better guide clinical medication,and provide new ideas for further study of its pathogenesis.
文摘Objective: to investigate the effects of long-life diet combined with bifidobacteria triple viable enteric-soluble capsules on glucose and lipid metabolism in patients with type 2 diabetes mellitus. Methods: in a double-cycle, crossover design, subjects were randomly divided into experimental group (macrobiotic diet combined with bifidobacterium triad live enteric-soluble capsule) and control group (diabetic dietary guidance). Each group lasted for 4 weeks, with a 4-week washout interval. Baseline and after the intervention were detected and compared two groups of participants' body weight (BW), body mass index (BMI), waist circumference (WC), hip circumference (HC), fasting plasma glucose (FPG), postprandial 2 hours blood glucose (2 HPBG), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL - C) and low density lipoprotein cholesterol ( HDL-C). Results: compared with the control group, the decrease of FPG, 2hPBG, TG, BW and BMI in the experimental group was statistically significant (P < 0.05). There was no statistical significance in the decrease of TC, HDL-C, LDL-C, WC and HC between the two groups (P > 0.05).Conclusion: the long-life diet combined with bifidobacterium triple viable enteric capsule can improve the blood glucose level, some lipid metabolism indicators and anthropometric indicators of type 2 diabetes patients.
文摘Objective: the purpose of this experiment is to study the effect of compound lizard powder gel on intestinal flora of rats with ulcerative colitis (UC). Methods: SPF male SD rats were randomly divided into 6 groups: blank group, model control group, compound lizard powder gel high, middle and low groups and sulfasalazine tablet treatment group. The clinical behavior of rats was observed daily during the modeling of TNBS, modeling period and drug intervention treatment period, and 16S rDNA high-throughput sequencing technique was used to compare and analyze the flora structure and bacteria genus differences in fresh feces of six groups of rats. Results: clinical behavior observation showed that the clinical condition of rats in the blank group was always good. In the model group, some rats had soft stools on the first day of modeling, and their spirit, activity and hair gradually changed. By the third day, the symptoms of loose stools and bloody stools gradually worsened. After treatment, the clinical symptoms of each group changed, but each group had its own characteristics. The sequencing results show that the current sequencing depth can fully reflect the diversity of microorganisms contained in the sample;PCoA analysis showed that the percentages of PCA1 and PCA2 in the score chart explaining population variance were 46.92% and 10.05% respectively. Multi-dimensional analysis shows that compared with the model control group and the blank group, the difference of flora composition between compound lizard powder group and sulfasalazine tablet treatment group is smaller. Analysis of the community structure of intestinal contents showed that the highest abundance in each group of samples at phylum level were phylum sclerenchyma and phycobacterium. After 14 days of drug intervention, the abundance of sclerenchyma and Bacteroides in compound lizard powder group and sulfasalazine tablet treatment group increased, and the abundance of Proteobacteria decreased, especially in compound Lizard powder middle dose group. In the control group, the abundance of degenerated phylum increased significantly. Genus levels, the highest abundance in each group of samples were Muribaculaceae_ unclassified and Lactobacillus. After 14 days of drug intervention, the abundance of Ruminococcaceae_UCG?005 in compound lizard powder group and sulfasalazine tablet treatment group increased significantly, and the abundance of Bacteroides in model control group was significantly higher than that in blank control group and compound lizard powder treatment groups. The lactic acid bacteria in the middle dosage group of compound lizard powder were higher than those in the high and low dosage groups of compound lizard powder and sulfasalazine tablets group. Conclusion: compound lizard powder gel, especially in the middle dosage group, is more effective in adjusting the intestinal flora structure and bacteria genus difference of UC.
基金supported by grants from the Young Elite Scientists Sponsorship Program by CAST(China Association for Science and technology),No.YESS 20160186.
文摘Background and Objectives:Observational studies have shown that energy restriction could be beneficial for controlling bodyweight in patients with polycystic ovary syndrome(PCOS).We aim to compare the effects of a high-protein diet(HPD),a high-protein and high-dietary fiber diet(HPHFD),and a calorie-restricted diet(CRD)on metabolic health and gut microbiota in overweight/obese PCOS patients.Methods and Study Design:We will enroll a total of 90 overweight/obese PCOS patients into this eight-week open-label randomised controlled trial.Participants will be randomly assigned to three groups:CRD group(energy coefficient 20 kcal/kg.day,water≥1500 mL,0.8-1.2 g/kg protein,carbohydrate energize 55-60%,and fat energize 25-30%),HDP group(energy coefficient 20 kcal/kg.day,water≥1500 mL,and 1.5-2.0 g/kg protein)and HPHFD group(based on the high protein diet with 15 g more dietary fiber supplement).The primary outcome is body weight,body fat percentage,and lean body mass.The secondary outcomes will include changes in blood lipids,inflammation,glucose tolerance,blood pressure,and gut microbiota compositions.Between-group differences in adiposity measurements at baseline will be compared using one-way analysis of variance(ANOVA)or Kruskal-Wallis test when appropriate.Within-group difference after 8-week intervention will be compared using paired t-test or Wilcoxon signed rank test.Between-group differences in adiposity measurements after 8-week diet intervention will be compared using linear mixed model and ANCOVA.The gut microbiota will be analyzed using 16S amplicon sequencing and the sequencing data will be analyzed using the standardized QIIME2 piperline.
