The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by ...The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently constructed for analysis,the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group.In addition,analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial mucosal immunity.展开更多
Objective To explore the role of curcumin(Cur)in improving IgA nephropathy(IgAN)and its related mechanisms.Methods Fifty 7-month-old miR-23b knockout(miR-23b^(-/-))mice weighing(25±5)g were used to establish an I...Objective To explore the role of curcumin(Cur)in improving IgA nephropathy(IgAN)and its related mechanisms.Methods Fifty 7-month-old miR-23b knockout(miR-23b^(-/-))mice weighing(25±5)g were used to establish an IgAN disease model,and were randomly divided into IgAN group,IgAN+Cur(150 mg/kg)group and IgAN+Cur(300 mg/kg)group using simple randomisation.展开更多
The protective effects of molecular crosslinked ready-to-eat sea cucumber(RSC)against immunosuppression induced by cyclophosphamide in mice were investigated.After gavage of molecular crosslinked RSC,the SIgA levels o...The protective effects of molecular crosslinked ready-to-eat sea cucumber(RSC)against immunosuppression induced by cyclophosphamide in mice were investigated.After gavage of molecular crosslinked RSC,the SIgA levels of intestinal mucosa in mice were significantly increased by 31.6%(P<0.01),and the terminal differentiation of IgA+B cell was promoted with the significant up-regulation of IL-6 and IL-10 levels.Moreover,the secretion mechanism of plasma cell was mainly promoted through XBP-1s pathway mediated by IRE1(P<0.01),rather than ATF-4 and ATF-6 pathway(P>0.05).In addition,the diversity of intestinal flora was increased,and the imbalance of intestinal flora was improved.The Lachnospiraceae_NK4A136_group dominated in crosslinked group could promote the repair of intestinal mucosa.Therefore,molecular crosslinked RSC can alleviate the intestinal mucosal damage via promoting SIgA secretion and regulating gut microbiota of mice.展开更多
At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at...At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at weaning ages which are common on commercial farms. At weaning piglets are presented with a vast and diverse range of microbial and dietary/environmental antigens. Their ability to distinguish between antigens and mount a protective response to potential pathogens and to develop tolerance to dietary antigens is critical to their survival and failure to do so is reflected in the high incidence of morbidity and mortality in the post-weaning period. A growing recognition that the widespread use of antibiotics to control infection during this critical period should be controlled has led to detailed studies of those factors which drive the development of the mucosal immune system, the role of gut microbiota in driving this process, the origin of the bacteria that colonise the young piglet＇s intestine and the impact of rearing environment. This review briefly describes how the mucosal immune system is equipped to respond ＂appropriately＂ to antigenic challenge and the programmed sequence by which it develops. The results of studies on the critical interplay between the host immune system and gut microbiota are discussed along with the effects of rearing environment. By comparing these with results from human studies on the development of allergies in children, an approach to promote an earlier maturation of the piglet immune system to resist the challenges of weaning are outlined.展开更多
Human milk oligosaccharides(HMOs)are critical bioactive glycans that modulate gut microbiota composition and intestinal mucosal immunity.Among them,2′-fucosyllactose(2′-FL)and lacto-N-neotetraose(LNnT)are predominan...Human milk oligosaccharides(HMOs)are critical bioactive glycans that modulate gut microbiota composition and intestinal mucosal immunity.Among them,2′-fucosyllactose(2′-FL)and lacto-N-neotetraose(LNnT)are predominant structures and major fermentable substrates for microbial production of short-chain fatty acids(SCFAs).To elucidate the downstream immunomodulatory actions of HMOs,this study specifically examined their principal microbial metabolites—SCFAs(acetate,propionate,and butyrate)—which function as central mediators of intestinal barrier integrity and immune homeostasis.This study investigated the protective effects and molecular mechanisms of SCFAs—particularly butyrate—against lipopolysaccharide(LPS)-induced intestinal mucosal immune injury.In a humanized rat model,SCFAs supplementation(acetate,propionate,and butyrate)markedly alleviated body weight loss,reduced disease activity index(DAI)and histological damage,and decreased myeloperoxidase(MPO)activity.SCFAs improved intestinal barrier integrity by upregulating Claudin-1,Claudin-2,ZO-1,and Muc2 expression,while reducing serum D-lactate and diamine oxidase(DAO)levels.They also suppressed pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-2)and enhanced anti-inflammatory cytokines(IL-10,IL-4)and secretory IgA(sIgA)production.Transcriptomic and protein-protein interaction(PPI)analyses revealed that butyrate modulated colonic gene expression enriched in G protein-coupled receptor signaling and IgA immune network pathways,and mitigated inflammation by inhibiting the EGFR/STAT1/CXCL10/CCL2 and IL-6/STAT3 signaling axes.In vitro,butyrate significantly reduced lactate dehydrogenase(LDH)release,downregulated inflammatory gene expression,and inhibited M1 macrophage polarization.Collectively,these findings indicate that HMOs confer intestinal immunoprotective effects primarily through the microbial metabolite butyrate,providing new mechanistic insights into the immunomodulatory potential of HMOs and supporting their development as functional food ingredients.展开更多
Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic...Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic and intestinal mucosal immunity remain poorly understood.This study comprehensively evaluated the immunomodulatory potential of D.indusiate peptide hydrolysate(DIPH)using a multi-omics approach.LC-MS/MS analysis showed that DIPH is composed of peptides containing 8 to 20 amino acid residues,with branchedchain amino acids comprising 20.53%of the total,and exhibits primarily hydrophobic properties.In cyclophosphamide-induced immunosuppressed mice,DIPH exhibited broad restorative effects on immunity via three interrelated mechanisms.Firstly,systemic immune function was enhanced,as demonstrated by significant elevations in serum IgG and IgM levels,and an improvement(4.5-fold)in carbon clearance capacity.Secondly,intestinal barrier integrity was strengthened through the upregulation of tight junction proteins,a 24.7%rise in goblet cells,and a 1.11-fold increase in secretory IgA production.Thirdly,DIPH administration modulated gut microbiota composition,significantly enriching beneficial bacteria including Bifidobacterium and Roseburia while reducing Streptococcus,which was accompanied by a 2.1-fold increase in butyrate production.At the molecular level,DIPH exerted its effects through dual regulatory mechanisms:suppression of the TLR4/MyD88/NF-κB pathway and activation of FoxO1-mediated mucosal restoration.These insights collectively establish DIPH as a multi-target edible fungi-derived immunomodulator that uniquely integrates gut microbiota regulation,systemic immune enhancement,and intestinal barrier restoration,highlighting its promising applications in functional food development and microbiome-targeted therapies.展开更多
Objective:To investigate the dynamic regulation of self-assembled aggregations(SAA)in Coptidis Rhizoma decoction on the permeability of intestinal tissue and the mechanism underlying.Methods:The effects of SAA on berb...Objective:To investigate the dynamic regulation of self-assembled aggregations(SAA)in Coptidis Rhizoma decoction on the permeability of intestinal tissue and the mechanism underlying.Methods:The effects of SAA on berberine(Ber)absorption were respectively analyzed in an in situ intestinal perfusion model and in an Ussing Chamber jejunum model with or without Peyer’s patches(PPs).The expression levels of ZO-1,Occludin and Claudin-1 were detected by immunofluorescence to evaluate the tight junction(TJ)between intestinal epithelium cells.The expression levels of T-box-containing protein expressed in T cells,signal transducers and activators of tranion-6,retinoic acid receptor-related orphan receptor ct and forkhead box P3 in PPs were detected by the reverse transcription-polymerase chain reaction and the secretions of interferon-c(IFN-c),interleukin-4(IL-4),interleukin-17(IL-17)and transforming growth factor-b(TGF-b)in PPs were evaluated by immunohistochemistry,to reflect the differentiation of T lymphocyte in PPs to helper T(Th)cell 1,Th2,Th17 and regulatory T(Treg)cell.To confirm the correlation between SAA in Coptidis Rhizoma decoction,PPs-associated immunity and intestinal epithelium permeability,SAA were administrated on an Ussing Chamber jejunum model with immunosuppressed PPs and evaluated its influences on intestinal tissue permeability and TJ proteins expression.Results:SAA in Coptidis Rhizoma decoction could dose-dependently promote Ber absorption in jejunum segment,with the participation of PPs.The dose-dependent and dynamical regulations of SAA on permeability of intestinal tissue and TJ proteins expression level between intestinal epithelium cells occurred along with the dynamically changed T lymphocyte differentiation and immune effectors secretion in PPs.The administration of SAA on immunosuppressed PPs exhibited dose-dependent PPs activation,inducing dynamic promotion on intestinal tissue permeability and inhibition on TJ proteins expression.Conclusion:SAA can improve the Ber absorption in small intestine,through the PPs-associated immunity induced dynamic regulation on intestinal tissue permeability and TJ proteins expression.These findings might enlighten the research of traditional Chinese medicine decoction.展开更多
With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal m...With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal mucosal immune system.Probiotics and their metabolites can interact with immune cells and gut microbiota to alleviate food allergy.This review outlines the relationship between the intestinal mucosal immune system and food allergy.This review also presents the clinical application and potential immunomodulation mechanisms of probiotics on food allergy.We aim at providing a reference for further studies to explore the key active substances and immunomodulation mechanisms of anti-allergic probiotics.展开更多
基金supported by National Key Research and Development Program of China(2019YFC1605003-3)the Science and Technology Projects of Xiamen Science and Technology Bureau(3502Z20183034)。
文摘The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently constructed for analysis,the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group.In addition,analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial mucosal immunity.
文摘Objective To explore the role of curcumin(Cur)in improving IgA nephropathy(IgAN)and its related mechanisms.Methods Fifty 7-month-old miR-23b knockout(miR-23b^(-/-))mice weighing(25±5)g were used to establish an IgAN disease model,and were randomly divided into IgAN group,IgAN+Cur(150 mg/kg)group and IgAN+Cur(300 mg/kg)group using simple randomisation.
基金supported by Major Scientific and Technological Innovation Project in Shandong Province(No.2022CXGC020414)Major Scientific and Technological Innovation Project of Qingdao West Coast New Area(No.ZDKC-2022-01)+1 种基金National Natural Science Foundation of China(No.31772046)Hainan province R&D projects(No.ZDYF2024XDNY190)and Taishan Scholar Foundation of Shandong Province(No.tsqn202103033).
文摘The protective effects of molecular crosslinked ready-to-eat sea cucumber(RSC)against immunosuppression induced by cyclophosphamide in mice were investigated.After gavage of molecular crosslinked RSC,the SIgA levels of intestinal mucosa in mice were significantly increased by 31.6%(P<0.01),and the terminal differentiation of IgA+B cell was promoted with the significant up-regulation of IL-6 and IL-10 levels.Moreover,the secretion mechanism of plasma cell was mainly promoted through XBP-1s pathway mediated by IRE1(P<0.01),rather than ATF-4 and ATF-6 pathway(P>0.05).In addition,the diversity of intestinal flora was increased,and the imbalance of intestinal flora was improved.The Lachnospiraceae_NK4A136_group dominated in crosslinked group could promote the repair of intestinal mucosa.Therefore,molecular crosslinked RSC can alleviate the intestinal mucosal damage via promoting SIgA secretion and regulating gut microbiota of mice.
文摘At birth the piglet＇s immune system is immature and it is dependent upon passive maternal protection until weaning.The piglet＇s mucosal immune system develops over the first few weeks but has not reached maturity at weaning ages which are common on commercial farms. At weaning piglets are presented with a vast and diverse range of microbial and dietary/environmental antigens. Their ability to distinguish between antigens and mount a protective response to potential pathogens and to develop tolerance to dietary antigens is critical to their survival and failure to do so is reflected in the high incidence of morbidity and mortality in the post-weaning period. A growing recognition that the widespread use of antibiotics to control infection during this critical period should be controlled has led to detailed studies of those factors which drive the development of the mucosal immune system, the role of gut microbiota in driving this process, the origin of the bacteria that colonise the young piglet＇s intestine and the impact of rearing environment. This review briefly describes how the mucosal immune system is equipped to respond ＂appropriately＂ to antigenic challenge and the programmed sequence by which it develops. The results of studies on the critical interplay between the host immune system and gut microbiota are discussed along with the effects of rearing environment. By comparing these with results from human studies on the development of allergies in children, an approach to promote an earlier maturation of the piglet immune system to resist the challenges of weaning are outlined.
基金supported by the Hohhot City"Government,Industry,University,Research,Promotion and Application of Silver"Innovation Consortium Project(2023RC-Consortium-7)the"Lutein/DHA-caseinalgal ternary composite small intestine-targeted controlled-release system and its application in eye-protecting functional food"Project(2025YFHH0187)the"Special Funding Project of the China Post-doctoral Science Foundation"(2025T180813).
文摘Human milk oligosaccharides(HMOs)are critical bioactive glycans that modulate gut microbiota composition and intestinal mucosal immunity.Among them,2′-fucosyllactose(2′-FL)and lacto-N-neotetraose(LNnT)are predominant structures and major fermentable substrates for microbial production of short-chain fatty acids(SCFAs).To elucidate the downstream immunomodulatory actions of HMOs,this study specifically examined their principal microbial metabolites—SCFAs(acetate,propionate,and butyrate)—which function as central mediators of intestinal barrier integrity and immune homeostasis.This study investigated the protective effects and molecular mechanisms of SCFAs—particularly butyrate—against lipopolysaccharide(LPS)-induced intestinal mucosal immune injury.In a humanized rat model,SCFAs supplementation(acetate,propionate,and butyrate)markedly alleviated body weight loss,reduced disease activity index(DAI)and histological damage,and decreased myeloperoxidase(MPO)activity.SCFAs improved intestinal barrier integrity by upregulating Claudin-1,Claudin-2,ZO-1,and Muc2 expression,while reducing serum D-lactate and diamine oxidase(DAO)levels.They also suppressed pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-2)and enhanced anti-inflammatory cytokines(IL-10,IL-4)and secretory IgA(sIgA)production.Transcriptomic and protein-protein interaction(PPI)analyses revealed that butyrate modulated colonic gene expression enriched in G protein-coupled receptor signaling and IgA immune network pathways,and mitigated inflammation by inhibiting the EGFR/STAT1/CXCL10/CCL2 and IL-6/STAT3 signaling axes.In vitro,butyrate significantly reduced lactate dehydrogenase(LDH)release,downregulated inflammatory gene expression,and inhibited M1 macrophage polarization.Collectively,these findings indicate that HMOs confer intestinal immunoprotective effects primarily through the microbial metabolite butyrate,providing new mechanistic insights into the immunomodulatory potential of HMOs and supporting their development as functional food ingredients.
基金supported by the National Key R&D Pro-gram of China(2023YFD1600503)the Key Research and Development Project of Jiangxi Province(20192ACB60008).
文摘Dictyophora indusiate(DI)contains numerous bioactive compounds with immunomodulatory properties.However,the functional activities of its protein hydrolysate and their mechanisms of action in the regulation of systemic and intestinal mucosal immunity remain poorly understood.This study comprehensively evaluated the immunomodulatory potential of D.indusiate peptide hydrolysate(DIPH)using a multi-omics approach.LC-MS/MS analysis showed that DIPH is composed of peptides containing 8 to 20 amino acid residues,with branchedchain amino acids comprising 20.53%of the total,and exhibits primarily hydrophobic properties.In cyclophosphamide-induced immunosuppressed mice,DIPH exhibited broad restorative effects on immunity via three interrelated mechanisms.Firstly,systemic immune function was enhanced,as demonstrated by significant elevations in serum IgG and IgM levels,and an improvement(4.5-fold)in carbon clearance capacity.Secondly,intestinal barrier integrity was strengthened through the upregulation of tight junction proteins,a 24.7%rise in goblet cells,and a 1.11-fold increase in secretory IgA production.Thirdly,DIPH administration modulated gut microbiota composition,significantly enriching beneficial bacteria including Bifidobacterium and Roseburia while reducing Streptococcus,which was accompanied by a 2.1-fold increase in butyrate production.At the molecular level,DIPH exerted its effects through dual regulatory mechanisms:suppression of the TLR4/MyD88/NF-κB pathway and activation of FoxO1-mediated mucosal restoration.These insights collectively establish DIPH as a multi-target edible fungi-derived immunomodulator that uniquely integrates gut microbiota regulation,systemic immune enhancement,and intestinal barrier restoration,highlighting its promising applications in functional food development and microbiome-targeted therapies.
基金supported by the National Natural Science Foundation of China(grant numbers 81874348,81303239)the Natural Science Foundation of Anhui Province(grant numbers 1908085J29)+3 种基金the Key Research and Development Plan of Anhui Province(grant number 201904b11020023)the Open Project of State Key Laboratory of Natural Medicines(grant number SKLNMKF202007)the Provincial Foundation for Excellent Young Talents of Colleges and Universities of Anhui Province(grant number gxyqZD2018052)the Anhui Provincial Department of Education(grant number KJ2018A0282)。
文摘Objective:To investigate the dynamic regulation of self-assembled aggregations(SAA)in Coptidis Rhizoma decoction on the permeability of intestinal tissue and the mechanism underlying.Methods:The effects of SAA on berberine(Ber)absorption were respectively analyzed in an in situ intestinal perfusion model and in an Ussing Chamber jejunum model with or without Peyer’s patches(PPs).The expression levels of ZO-1,Occludin and Claudin-1 were detected by immunofluorescence to evaluate the tight junction(TJ)between intestinal epithelium cells.The expression levels of T-box-containing protein expressed in T cells,signal transducers and activators of tranion-6,retinoic acid receptor-related orphan receptor ct and forkhead box P3 in PPs were detected by the reverse transcription-polymerase chain reaction and the secretions of interferon-c(IFN-c),interleukin-4(IL-4),interleukin-17(IL-17)and transforming growth factor-b(TGF-b)in PPs were evaluated by immunohistochemistry,to reflect the differentiation of T lymphocyte in PPs to helper T(Th)cell 1,Th2,Th17 and regulatory T(Treg)cell.To confirm the correlation between SAA in Coptidis Rhizoma decoction,PPs-associated immunity and intestinal epithelium permeability,SAA were administrated on an Ussing Chamber jejunum model with immunosuppressed PPs and evaluated its influences on intestinal tissue permeability and TJ proteins expression.Results:SAA in Coptidis Rhizoma decoction could dose-dependently promote Ber absorption in jejunum segment,with the participation of PPs.The dose-dependent and dynamical regulations of SAA on permeability of intestinal tissue and TJ proteins expression level between intestinal epithelium cells occurred along with the dynamically changed T lymphocyte differentiation and immune effectors secretion in PPs.The administration of SAA on immunosuppressed PPs exhibited dose-dependent PPs activation,inducing dynamic promotion on intestinal tissue permeability and inhibition on TJ proteins expression.Conclusion:SAA can improve the Ber absorption in small intestine,through the PPs-associated immunity induced dynamic regulation on intestinal tissue permeability and TJ proteins expression.These findings might enlighten the research of traditional Chinese medicine decoction.
基金supported by the National Key Research and Development Program of China(2019YFC1605000)the National Natural Science Foundation(31872904)。
文摘With the prevalence of food allergy increasing every year,food allergy has become a common public health problem.More and more studies have shown that probiotics can intervene in food allergy based on the intestinal mucosal immune system.Probiotics and their metabolites can interact with immune cells and gut microbiota to alleviate food allergy.This review outlines the relationship between the intestinal mucosal immune system and food allergy.This review also presents the clinical application and potential immunomodulation mechanisms of probiotics on food allergy.We aim at providing a reference for further studies to explore the key active substances and immunomodulation mechanisms of anti-allergic probiotics.
