Soil continuous monocropping obstacles pose a significant challenge to the sustainable production of cut chrysanthemums.Yet,the effectiveness of integrating biochar and microbial antagonists in alleviating these obsta...Soil continuous monocropping obstacles pose a significant challenge to the sustainable production of cut chrysanthemums.Yet,the effectiveness of integrating biochar and microbial antagonists in alleviating these obstacles in cut chrysanthemum production remains unclear.Here,we collected soils from a 12-year continuous cropping system with a high incidence of disease to establish a pot experiment comprising four treatments:control(CK),biochar(BC),Bacillus subtilis(BM),and their combined addition(BM_BC),investigating the effects of biochar and B.subtilis on the disease incidence,plant growth,pathogenic and antagonistic microbial populations,and the bacterial and fungal communities in diseased soil.The results showed that BM_BC treatment effectively controlled the disease and significantly increased(P<0.05)the plant biomass and root activity of cut chrysanthemum by 41.3%and 254%,respectively,compared to the CK.Notably,the BM_BC exhibited the lowest population of Fusarium oxysporum and the highest population of B.subtilis,along with the greatest alpha diversity(measured by Chao1 and Shannon indices)of both bacterial and fungal communities among the four treatments.The amendments of BC,BM,and BM_BC significantly altered the structure and composition of bacterial and fungal communities,with BM_BC primarily enriching beneficial bacteria and suppressing pathogen.Microbial co-occurrence network analysis revealed that BM_BC increased the abundance of module 2,co-dominated by bacterial and fungal species,and strengthened the interactions between them.The PLS-PM analysis demonstrated that bacteria-fungi interkingdom interactions played a crucial role in promoting the growth of cut chrysanthemums in diseased soil.Therefore,our findings underscore the synergistic effects of biochar and B.subtilis in suppressing Fusarium wilt disease and enhancing the growth of cut chrysanthemums by strengthening microbial interkingdom interactions.展开更多
The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both partners.During the communication process,the contacted organisms produce and rel...The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both partners.During the communication process,the contacted organisms produce and release small molecules that establish communication channels between two kingdoms—this procedure is known as interkingdom signaling.Interkingdom communications are widespread between pathogenic or beneficial bacteria and their host plants,with diversified outcomes depending on the specific chemical-triggered signaling pathways.Deciphering the signals or language of this interkingdom communication and uncovering the underlying mechanisms are major current challenges in this field.It is evident that diverse signaling molecules can be produced or derived from bacteria and plants,and researchers have sought to identify these signals and explore the mechanisms of the signaling pathways.The results of such studies will lead to the development of strategies to improve plant disease resistance through controlling interkingdom signals,rather than directly killing the pathogenic bacteria.Also,the identification of signals produced by beneficial bacteria will be useful for agricultural applications.In this review,we summarize the recent progress of cross-kingdom interactions between plant and bacteria,and how LuxR-family transcription factors in plant associated bacterial quorum sensing system are involved in the interkingdom signaling.展开更多
Background The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma(ESCC).Methods Patients with primary ESCC were recruited...Background The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma(ESCC).Methods Patients with primary ESCC were recruited from two central hospitals.We performed internal transcribed spacer 1(ITS1)ribosomal DNA sequencing analysis.We compared differential fungi and explored the ecology of fungi and the interaction of bacteria and fungi.Results The mycobiota diversity was significantly different between tumors and tumor-adjacent samples.We further analysed the differences between the two groups,at the species level,confirming that Rhodotorula toruloides,Malassezia dermatis,Hanseniaspora lachancei,and Spegazzinia tessarthra were excessively colonized in the tumor samples,whereas Preussia persica,Fusarium solani,Nigrospora oryzae,Acremonium furcatum,Golovinomyces artemisiae,and Tausonia pullulans were significantlymore abundant in tumor-adjacent samples.The fungal co-occurrence network in tumor-adjacent samples was larger and denser than that in tumors.Similarly,themore complex bacterial–fungal interactions in tumor-adjacent samples were also detected.The expression ofmechanistic target of rapamycin kinase was positively correlated with the abundance of N.oryzae and T.pullulans in tumor-adjacent samples.In tumors,the expression ofMET proto-oncogene,receptor tyrosine kinase(MET)had a negative correlation and a positive correlation with the abundance of R.toruloides and S.tessarthra,respectively.Conclusion This study revealed the landscape of the esophageal mycobiome characterized by an altered fungal composition and bacterial and fungal ecology in ESCC.展开更多
基金supported by grants from National Natural Science Foundation of China(Grant No.42207367)the Basic Public Welfare Research Project of Zhejiang Province(No.LGN20C150003)China Postdoctoral Science Foundation funded project(No.2022M711655).
文摘Soil continuous monocropping obstacles pose a significant challenge to the sustainable production of cut chrysanthemums.Yet,the effectiveness of integrating biochar and microbial antagonists in alleviating these obstacles in cut chrysanthemum production remains unclear.Here,we collected soils from a 12-year continuous cropping system with a high incidence of disease to establish a pot experiment comprising four treatments:control(CK),biochar(BC),Bacillus subtilis(BM),and their combined addition(BM_BC),investigating the effects of biochar and B.subtilis on the disease incidence,plant growth,pathogenic and antagonistic microbial populations,and the bacterial and fungal communities in diseased soil.The results showed that BM_BC treatment effectively controlled the disease and significantly increased(P<0.05)the plant biomass and root activity of cut chrysanthemum by 41.3%and 254%,respectively,compared to the CK.Notably,the BM_BC exhibited the lowest population of Fusarium oxysporum and the highest population of B.subtilis,along with the greatest alpha diversity(measured by Chao1 and Shannon indices)of both bacterial and fungal communities among the four treatments.The amendments of BC,BM,and BM_BC significantly altered the structure and composition of bacterial and fungal communities,with BM_BC primarily enriching beneficial bacteria and suppressing pathogen.Microbial co-occurrence network analysis revealed that BM_BC increased the abundance of module 2,co-dominated by bacterial and fungal species,and strengthened the interactions between them.The PLS-PM analysis demonstrated that bacteria-fungi interkingdom interactions played a crucial role in promoting the growth of cut chrysanthemums in diseased soil.Therefore,our findings underscore the synergistic effects of biochar and B.subtilis in suppressing Fusarium wilt disease and enhancing the growth of cut chrysanthemums by strengthening microbial interkingdom interactions.
基金supported by National Basic Research Program of China(2015B150600)National Key R&D Program(2016YFD0100600)National Natural Science Foundation of China(31370161)
文摘The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both partners.During the communication process,the contacted organisms produce and release small molecules that establish communication channels between two kingdoms—this procedure is known as interkingdom signaling.Interkingdom communications are widespread between pathogenic or beneficial bacteria and their host plants,with diversified outcomes depending on the specific chemical-triggered signaling pathways.Deciphering the signals or language of this interkingdom communication and uncovering the underlying mechanisms are major current challenges in this field.It is evident that diverse signaling molecules can be produced or derived from bacteria and plants,and researchers have sought to identify these signals and explore the mechanisms of the signaling pathways.The results of such studies will lead to the development of strategies to improve plant disease resistance through controlling interkingdom signals,rather than directly killing the pathogenic bacteria.Also,the identification of signals produced by beneficial bacteria will be useful for agricultural applications.In this review,we summarize the recent progress of cross-kingdom interactions between plant and bacteria,and how LuxR-family transcription factors in plant associated bacterial quorum sensing system are involved in the interkingdom signaling.
基金from Nature Science Foundation of Fujian Province[no.2021J01733,no.2021J01726]central government-led local science and technology development special project[no.2019L3006,no.2020L3009]Startup Fund for scientific research,Fujian Medical University(no.2020QH2008).
文摘Background The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma(ESCC).Methods Patients with primary ESCC were recruited from two central hospitals.We performed internal transcribed spacer 1(ITS1)ribosomal DNA sequencing analysis.We compared differential fungi and explored the ecology of fungi and the interaction of bacteria and fungi.Results The mycobiota diversity was significantly different between tumors and tumor-adjacent samples.We further analysed the differences between the two groups,at the species level,confirming that Rhodotorula toruloides,Malassezia dermatis,Hanseniaspora lachancei,and Spegazzinia tessarthra were excessively colonized in the tumor samples,whereas Preussia persica,Fusarium solani,Nigrospora oryzae,Acremonium furcatum,Golovinomyces artemisiae,and Tausonia pullulans were significantlymore abundant in tumor-adjacent samples.The fungal co-occurrence network in tumor-adjacent samples was larger and denser than that in tumors.Similarly,themore complex bacterial–fungal interactions in tumor-adjacent samples were also detected.The expression ofmechanistic target of rapamycin kinase was positively correlated with the abundance of N.oryzae and T.pullulans in tumor-adjacent samples.In tumors,the expression ofMET proto-oncogene,receptor tyrosine kinase(MET)had a negative correlation and a positive correlation with the abundance of R.toruloides and S.tessarthra,respectively.Conclusion This study revealed the landscape of the esophageal mycobiome characterized by an altered fungal composition and bacterial and fungal ecology in ESCC.
