Nitrogen (N) is a crucial nutrient vital for the growth and productivity of maize. However, excessive nitrogen application can result in numerous environmental and ecological problems, such as water pollution, biodive...Nitrogen (N) is a crucial nutrient vital for the growth and productivity of maize. However, excessive nitrogen application can result in numerous environmental and ecological problems, such as water pollution, biodiversity loss, and greenhouse gas emissions. Therefore, breeding maize hybrids resilient to low nitrogen conditions is crucial for sustainable agriculture, especially under low nitrogen conditions. Consequently, this study aimed to evaluate the combining ability and heterosis of maize lines, recognize promising hybrids, and study gene action controlling key traits under low and recommended N stress conditions. The half-diallel mating design hybridized seven maize inbreds, resulting in 21 F1 hybrids. These hybrids, along with two high-yielding commercial hybrids (SC10 and TWC310), were evaluated in field trials under recommended (290 kg/ha) and low N (166 kg N/ha) conditions. Significant variations were observed among assessed hybrids for all measured traits, with non-additive gene action being predominant for grain yield and its related characteristics under recommended and low N conditions. Inbred lines P105 and P106 were recognized as effective combiners for earliness, with P105 also excelling in shorter plant height and lower ear placement. In addition, P101, P102, and P104 were identified as good combiners for increasing grain yield and related attributes under low N conditions. The crosses P105 × P106 and P106 × P107 demonstrated outstanding heterotic effects for earliness, while hybrids P101 × P102 and P102 × P104 exhibited remarkable heterotic effects for grain yield low nitrogen stress conditions. These promising hybrids could be considered for commercial use after further evaluation. Strong positive correlations were found between grain yield and ear height, plant height, number of kernels per row, and 1000-grain weight, highlighting their importance for indirect selection to enhance the grain yield of maize under low N stress conditions.展开更多
Challenges arise in global viticulture due to low temperatures.To ensure the sustainable and high-quality development of the wine industry,it is essential to breed wine grape varieties that are not only of high qualit...Challenges arise in global viticulture due to low temperatures.To ensure the sustainable and high-quality development of the wine industry,it is essential to breed wine grape varieties that are not only of high quality but also possess cold hardiness.Intraspecific recurrent selection in Vitis vinifera can enhance cold hardiness while maintaining fruit quality.In this study,we used‘Ecolly’as an intermediary grape variety for crossing with‘Cabernet Sauvignon’,‘Marselan’,and‘Dunkelfelder’,including three reciprocal crosses and a total of 1,657 intraspecific hybrids.We characterized the cold hardiness of these intraspecific hybrids and analyzed the genetic aspects of cold hardiness,ultimately identifying excellent strains with cold hardiness.Parameters like mean high-temperature exotherm(mHTE),mean low-temperature exotherm(mLTE),bound/free water ratio,water loss ratio in vitro,frost damage grades,and overall performance displayed partially normal distributions.In intraspecific hybrids,there was a maternal advantage in traits related to bound/free water ratio and water loss ratio.Some hybrid populations exhibited values for mHTE,mLTE,and water loss ratio that were lower than the low parent's values,while bound/free water ratio showed values higher than the high parent's values.Among the 1,657 intraspecific hybrids,52 strains could bud under stress at-18℃,and seven of these strains excelled in three important cold hardiness measures.Our study revealed that cold hardiness in V.vinifera is influenced by multiple genes and is a quantitative trait.Intraspecific hybridization can produce a small number of superior strains with enhanced cold hardiness.展开更多
Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study ...Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study aimed to assess 30 cotton hybrids for the stability of four traits(single-plant seed cotton yield,fiber upper half mean length(UHML),fiber strength,and micronaire)across three environments.Recent techniques,including genotype and genotype×environment(GGE)biplot,which provides a visual representation of performance and adaptability;weighted average absolute scores of the best linear unbiased predictions(WAASB),which balances the performance of the trait with stability;and multi-trait stability index(MTSI),which integrates multi-trait performance and stability,were used to analyze the stability of the four traits.Results Analysis of variance revealed significant genotype and environment interactions for all the traits studied,highlighting the need for comprehensive stability analysis.The environment E2 was the most suitable for the evaluation of seed cotton yield,whereas E3 was suitable for the evaluation of UHML and fiber strength.A stable hybrid,H05(TVH002×MCU5),with superior performance for seed cotton yield and UHML,was identified based on the overall results from GGE and WAASB.The which-won-where bioplot showed that H25(SVPR3×MCU5)performed the best for seed cotton yield in E3,and H27(Suraj×Sunantha)in E2 and E1.The hybrid H04(TVH002×CO14)in E1 and H30(Suraj×MCU5)in E2 and E3 performed well for UHML.Similarly,H28(Suraj×Suraksha)for E2 and E3 and H26(Suraj×Subiksha)for E1 were the best performing in the case of fiber strength.Based on the MTSI,four promising hybrids,namely,H24(SVPR3×CO14),H09(TVH2010×CO14),H18(MCU7×Suraksha),and H29(Suraj×CO14),were identified as stable with average performance for all four traits.Conclusions The study identified a stable hybrid,H05(TVH002×MCU5),with superior performance for yield and UHML.The identified hybrids in this study hold significant potential for cultivation across Tamil Nadu,with a scope for further evaluation in diverse environments.展开更多
In this study,an inverse design framework was established to find lightweight honeycomb structures(HCSs)with high impact resistance.The hybrid HCS,composed of re-entrant(RE)and elliptical annular re-entrant(EARE)honey...In this study,an inverse design framework was established to find lightweight honeycomb structures(HCSs)with high impact resistance.The hybrid HCS,composed of re-entrant(RE)and elliptical annular re-entrant(EARE)honeycomb cells,was created by constructing arrangement matrices to achieve structural lightweight.The machine learning(ML)framework consisted of a neural network(NN)forward regression model for predicting impact resistance and a multi-objective optimization algorithm for generating high-performance designs.The surrogate of the local design space was initially realized by establishing the NN in the small sample dataset,and the active learning strategy was used to continuously extended the local optimal design until the model converged in the global space.The results indicated that the active learning strategy significantly improved the inference capability of the NN model in unknown design domains.By guiding the iteration direction of the optimization algorithm,lightweight designs with high impact resistance were identified.The energy absorption capacity of the optimal design reached 94.98%of the EARE honeycomb,while the initial peak stress and mass decreased by 28.85%and 19.91%,respectively.Furthermore,Shapley Additive Explanations(SHAP)for global explanation of the NN indicated a strong correlation between the arrangement mode of HCS and its impact resistance.By reducing the stiffness of the cells at the top boundary of the structure,the initial impact damage sustained by the structure can be significantly improved.Overall,this study proposed a general lightweight design method for array structures under impact loads,which is beneficial for the widespread application of honeycomb-based protective structures.展开更多
In order to address environmental pollution and resource depletion caused by traditional power generation,this paper proposes an adaptive iterative dynamic-balance optimization algorithm that integrates the Improved D...In order to address environmental pollution and resource depletion caused by traditional power generation,this paper proposes an adaptive iterative dynamic-balance optimization algorithm that integrates the Improved Dung Beetle Optimizer(IDBO)with VariationalMode Decomposition(VMD).The IDBO-VMD method is designed to enhance the accuracy and efficiency of wind-speed time-series decomposition and to effectively smooth photovoltaic power fluctuations.This study innovatively improves the traditional variational mode decomposition(VMD)algorithm,and significantly improves the accuracy and adaptive ability of signal decomposition by IDBO selfoptimization of key parameters K and a.On this basis,Fourier transform technology is used to define the boundary point between high frequency and low frequency signals,and a targeted energy distribution strategy is proposed:high frequency fluctuations are allocated to supercapacitors to quickly respond to transient power fluctuations;Lowfrequency components are distributed to lead-carbon batteries,optimizing long-term energy storage and scheduling efficiency.This strategy effectively improves the response speed and stability of the energy storage system.The experimental results demonstrate that the IDBO-VMD algorithm markedly outperforms traditional methods in both decomposition accuracy and computational efficiency.Specifically,it effectively reduces the charge–discharge frequency of the battery,prolongs battery life,and optimizes the operating ranges of the state-of-charge(SOC)for both leadcarbon batteries and supercapacitors.In addition,the energy management strategy based on the algorithm not only improves the overall energy utilization efficiency of the system,but also shows excellent performance in the dynamic management and intelligent scheduling of renewable energy generation.展开更多
Carbon superstructures with multiscale hierarchies and functional attributes represent an appealing cathode candidate for zinc hybrid capacitors,but their tailor-made design to optimize the capacitive activity remains...Carbon superstructures with multiscale hierarchies and functional attributes represent an appealing cathode candidate for zinc hybrid capacitors,but their tailor-made design to optimize the capacitive activity remains a confusing topic.Here we develop a hydrogen-bond-oriented interfacial super-assembly strategy to custom-tailor nanosheet-intertwined spherical carbon superstructures(SCSs)for Zn-ion storage with double-high capacitive activity and durability.Tetrachlorobenzoquinone(H-bond acceptor)and dimethylbenzidine(H-bond donator)can interact to form organic nanosheet modules,which are sequentially assembled,orientally compacted and densified into well-orchestrated superstructures through multiple H-bonds(N-H···O).Featured with rich surface-active heterodiatomic motifs,more exposed nanoporous channels,and successive charge migration paths,SCSs cathode promises high accessibility of built-in zincophilic sites and rapid ion diffusion with low energy barriers(3.3Ωs-0.5).Consequently,the assembled Zn||SCSs capacitor harvests all-round improvement in Zn-ion storage metrics,including high energy density(166 Wh kg-1),high-rate performance(172 m Ah g^(-1)at 20 A g^(-1)),and long-lasting cycling lifespan(95.5%capacity retention after 500,000 cycles).An opposite chargecarrier storage mechanism is rationalized for SCSs cathode to maximize spatial capacitive charge storage,involving high-kinetics physical Zn^(2+)/CF_(3)SO_(3)-adsorption and chemical Zn^(2+)redox with carbonyl/pyridine groups.This work gives insights into H-bond-guided interfacial superassembly design of superstructural carbons toward advanced energy storage.展开更多
Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant chal...Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant challenges in privacy-sensitive and distributed settings,often neglecting label dependencies and suffering from low computational efficiency.To address these issues,we introduce a novel framework,Fed-MFSDHBCPSO—federated MFS via dual-layer hybrid breeding cooperative particle swarm optimization algorithm with manifold and sparsity regularization(DHBCPSO-MSR).Leveraging the federated learning paradigm,Fed-MFSDHBCPSO allows clients to perform local feature selection(FS)using DHBCPSO-MSR.Locally selected feature subsets are encrypted with differential privacy(DP)and transmitted to a central server,where they are securely aggregated and refined through secure multi-party computation(SMPC)until global convergence is achieved.Within each client,DHBCPSO-MSR employs a dual-layer FS strategy.The inner layer constructs sample and label similarity graphs,generates Laplacian matrices to capture the manifold structure between samples and labels,and applies L2,1-norm regularization to sparsify the feature subset,yielding an optimized feature weight matrix.The outer layer uses a hybrid breeding cooperative particle swarm optimization algorithm to further refine the feature weight matrix and identify the optimal feature subset.The updated weight matrix is then fed back to the inner layer for further optimization.Comprehensive experiments on multiple real-world multi-label datasets demonstrate that Fed-MFSDHBCPSO consistently outperforms both centralized and federated baseline methods across several key evaluation metrics.展开更多
The hybridization gap in strained-layer InAs/In_(x)Ga_(1−x) Sb quantum spin Hall insulators(QSHIs)is significantly enhanced compared to binary InAs/GaSb QSHI structures,where the typical indium composition,x,ranges be...The hybridization gap in strained-layer InAs/In_(x)Ga_(1−x) Sb quantum spin Hall insulators(QSHIs)is significantly enhanced compared to binary InAs/GaSb QSHI structures,where the typical indium composition,x,ranges between 0.2 and 0.4.This enhancement prompts a critical question:to what extent can quantum wells(QWs)be strained while still preserving the fundamental QSHI phase?In this study,we demonstrate the controlled molecular beam epitaxial growth of highly strained-layer QWs with an indium composition of x=0.5.These structures possess a substantial compressive strain within the In_(0.5)Ga_(0.5)Sb QW.Detailed crystal structure analyses confirm the exceptional quality of the resulting epitaxial films,indicating coherent lattice structures and the absence of visible dislocations.Transport measurements further reveal that the QSHI phase in InAs/In_(0.5)Ga_(0.5)Sb QWs is robust and protected by time-reversal symmetry.Notably,the edge states in these systems exhibit giant magnetoresistance when subjected to a modest perpendicular magnetic field.This behavior is in agreement with the𝑍2 topological property predicted by the Bernevig–Hughes–Zhang model,confirming the preservation of topologically protected edge transport in the presence of enhanced bulk strain.展开更多
To enhance power flow regulation in scenarios involving large-scale renewable energy transmission via high-voltage direct current(HVDC)links and multi-infeed DC systems in load-center regions,this paper proposes a hyb...To enhance power flow regulation in scenarios involving large-scale renewable energy transmission via high-voltage direct current(HVDC)links and multi-infeed DC systems in load-center regions,this paper proposes a hybrid modular multilevel converter–capacitor-commutated line-commutated converter(MMC-CLCC)HVDC transmission system and its corresponding control strategy.First,the system topology is constructed,and a submodule configuration method for the MMC—combining full-bridge submodules(FBSMs)and half-bridge submodules(HBSMs)—is proposed to enable direct power flow reversal.Second,a hierarchical control strategy is introduced,includingMMCvoltage control,CLCC current control,and a coordinationmechanism,along with the derivation of the hybrid system’s power flow reversal characteristics.Third,leveraging the CLCC’s fast current regulation and theMMC’s negative voltage control capability,a coordinated power flow reversal control strategy is developed.Finally,an 800 kV MMC-CLCC hybrid HVDC system is modeled in PSCAD/EMTDC to validate the power flow reversal performance under a high proportion of full-bridge submodule configuration.Results demonstrate that the proposed control strategy enables rapid(1-s transition)and smooth switching of bidirectional power flow without modifying the structure of primary equipment:the transient fluctuation ofDC voltage from the rated value(UdcN)to themaximumreverse voltage(-kUdcN)is less than 5%;the DC current strictly follows the preset characteristic curve with a deviation of≤3%;the active power reverses continuously,and the system maintains stable operation throughout the reversal process.展开更多
[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River...[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River stock ♂ ）, ZR （Zhujiang River stock ♀ ×Zhujiang River stock♂ ） and hybrids F1 （ Yangtze River stock ♀ × Zhujiang River stock ♂ ） were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations （ P〉0. 05）, while there was an extremely significantly difference in those of 170 day-old generations （ P 〈0.01 ）. However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR （ P 〉 0.05）, but significant difference between F1 and YR （ P 〈 0.05）. F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length （ P 〉 0.05）, while significant difference in head length/total length, body height/standard length and body width/standard length （ P 〈 0.05）, which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.展开更多
[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Re...[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.展开更多
To understand the inter-hybridization between Cucumis ssp.plants,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but see...To understand the inter-hybridization between Cucumis ssp.plants,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but seeds from fruits V2（V129）×C.metuliferus were abortive.A few of seeds from the bottom of fruit V2（V129）×C.anguria were fertile.Sequence-related amplified polymorphism（SRAP）molecular markers were used to analyze the progenies of inter-specific hybridization between C.anguria and melon V129.One pair primer（E14/M2）was found effective in amplification on male parent characteristic bands from the hybrids,suggesting that some DNA exchange had happened between C.anguria and melon V129.This study provided data for analyzing the mechanism of inter-hybridization between Cucumis plants.展开更多
[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or wit...Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confirm the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis + A. deliciosa) and four clones (A. chinensis + A. kolomikta) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis + A. deliciosa) clone were deduced as octoploid (2n = 8x), and the (A. chinensis + A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n = 5x). The clones were confirmed as interspecific somatic hybrids in Actinidia.展开更多
Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs ...Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.展开更多
[Objective] This study aimed to analyze the influence of five cultivation factors： sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on oil quality and ...[Objective] This study aimed to analyze the influence of five cultivation factors： sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on oil quality and stability of Brassica napus hybrids. [Method] Statistical analysis was carried out based on the data from previous studies and our work. [Result] Sowing date had no significant influence on oil quality and stability of B. napus hybrids. The plant density shared a positive correlation with 18-C fatty acid （FA） content, unsaturated fatty acid （UFA） content, total content of linoleic acid and linolenic acid, index of unsaturated fatty acid （IUFA）, so a large plant density can improve the oil quality （by increasing18-C FA content, UFA content, total content of linoleic acid and linolenic acid）, but reduce the oil stability （by increasing IUFA）. The amount of nitrogen fertilizer had no significant influence on the contents of 18-C FA and UFA, but significant positive correlations with the total content of linoleic acid and linolenic acid and IUFA. So, the total content of linoleic acid and linolenic acid can be reduced by increasing nitrogen amount, meanwhile the oil stability was also decreased. Phosphate fertilizer amount did not affect 18-C FA significantly, but was negatively correlated with the UFA content, total content of linoleic acid and linolenic acid, and IUFA. So, increasing the amount of applied phosphate fertilizer can reduce UFA content, total content of linoleic acid and linolenic acid, thereby improving oil stability. Potash fertilizer had no significant influence on the IUFA, a significant positive correlation with 18-C FA content, an insignificant positive correlation with the the UFA content, and a negative correlation with the total content of linoleic acid and linolenic acid. The oil content of rapeseed shared an extremely significant positive correlation with 18-C FA content and UFA content, an negative correlation with the total content of linoleic acid and linolenic acid, and no significant correlation with IUFA. [Conclusion] This study identified the effects of five main cultivation factors on oil quality and stability of B. napus hybrids, and the correlations of oil content with 18-C FA content, UFA content, total content of linoleic acid and linolenic acid, and IUFA.展开更多
The combined lines having both phKL and Ph2-deficiency were obtained in the genetic background of common wheat (Triticum aestivum L.) landrace. These lines had normal fertility. In the wheat combined lines X Aegilops ...The combined lines having both phKL and Ph2-deficiency were obtained in the genetic background of common wheat (Triticum aestivum L.) landrace. These lines had normal fertility. In the wheat combined lines X Aegilops variabilis Eig. (or rye), a significant increase in the chiasmata of homoeologous pairing was shown by the phKL+Ph2(-) plants with respect to their phKL+Ph2 sibs, which indicates that Ph2-deficiency and phKL showed an additive effect on promoting pairing. The effects were shown in the increment of rod bivalents, ring bivalents and trivalents and reduction of univalents, of which, reduction of univalents was mainly due to the increment of rod bivalents. The combined lines are probably more desirable materials for alien gene transferring than phKL or Ph2(-) lines alone. In comparison with that of ph1b X Ae. variabilis (or rye), phKL+Ph2(-) X Ae. variabilis (or rye) show higher (or similar) numbers of rod bivalents, while the total chromosome pairing level significantly reduced that ascribed to the decrement in ring bivalents and multivalents. These results probably indicate the different genetic mechanisms for Ph1 and Ph2 or phKL on controlling homoeologous pairing.展开更多
The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivisio...The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background.展开更多
[Objective] This study aimed to investigate the reason for the different leaf colors of pomegranate hybrid progeny. [Method] Spectrophotometry was used to determine the contents of three pigments, including chlorophyl...[Objective] This study aimed to investigate the reason for the different leaf colors of pomegranate hybrid progeny. [Method] Spectrophotometry was used to determine the contents of three pigments, including chlorophyll, carotenoid and anthocyanin, in the leaves of eight pomegranate individuals and their parents at growth stage. [Result] The results showed that during the early growth stage, the anthocyanin contents of four pomegranate individuals with red leaves were higher than the control, to be specific, the anthocyanin content of No.5 pomegranate individual reached the highest of 4.12 mg/g; the chlorophyll and carotenoid contents of four pomegranate individuals with yellow-green leaves showed consistent dynamic trends with the control, to be specific, the chlorophyll content and chlorophyll/carotenoid ratio of No.4 pomegranate individual reached the highest, while the chlorophyll a/carotenoid b ratio of No.3 pomegranate individual was the lowest. During vigorous growth stage, the contents of chlorophyll, carotenoid and anthocyanin of eight pomegranate individuals showed consistent dynamic trends, with no significant difference with the control. Analysis of variance indicated that there were significant differences in the contents of chlorophyll and carotenoid among different pomegranate individuals at different growth stages and in the content of anthocyanin among different pomegranate individuals, while no significant difference was observed in the chlorophyll/carotenoid ratio among different pomegranate individuals at different growth stages. [Conclusion] The differences in leaf colors among pomegranate hybrid progeny are caused by different contents and ratios of anthocyanin and chlorophyll at growth stage.展开更多
基金Princess Nourah bint Abdulrahman University Research Supporting Project Number PNURSP2025R241,Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Nitrogen (N) is a crucial nutrient vital for the growth and productivity of maize. However, excessive nitrogen application can result in numerous environmental and ecological problems, such as water pollution, biodiversity loss, and greenhouse gas emissions. Therefore, breeding maize hybrids resilient to low nitrogen conditions is crucial for sustainable agriculture, especially under low nitrogen conditions. Consequently, this study aimed to evaluate the combining ability and heterosis of maize lines, recognize promising hybrids, and study gene action controlling key traits under low and recommended N stress conditions. The half-diallel mating design hybridized seven maize inbreds, resulting in 21 F1 hybrids. These hybrids, along with two high-yielding commercial hybrids (SC10 and TWC310), were evaluated in field trials under recommended (290 kg/ha) and low N (166 kg N/ha) conditions. Significant variations were observed among assessed hybrids for all measured traits, with non-additive gene action being predominant for grain yield and its related characteristics under recommended and low N conditions. Inbred lines P105 and P106 were recognized as effective combiners for earliness, with P105 also excelling in shorter plant height and lower ear placement. In addition, P101, P102, and P104 were identified as good combiners for increasing grain yield and related attributes under low N conditions. The crosses P105 × P106 and P106 × P107 demonstrated outstanding heterotic effects for earliness, while hybrids P101 × P102 and P102 × P104 exhibited remarkable heterotic effects for grain yield low nitrogen stress conditions. These promising hybrids could be considered for commercial use after further evaluation. Strong positive correlations were found between grain yield and ear height, plant height, number of kernels per row, and 1000-grain weight, highlighting their importance for indirect selection to enhance the grain yield of maize under low N stress conditions.
基金supported by Project of Ningxia Hui Autonomous Region Key R&D Program(Grant No.NXNYYZ202101)the National Natural Science Foundation of China(Grant No.32360804)+1 种基金Ningxia Science and Technology Department major scientific and technological achievements transformation project(Grant No.2022CJE9007)Ningxia Natural Science Foundation(Grant No.2023AAC03064).
文摘Challenges arise in global viticulture due to low temperatures.To ensure the sustainable and high-quality development of the wine industry,it is essential to breed wine grape varieties that are not only of high quality but also possess cold hardiness.Intraspecific recurrent selection in Vitis vinifera can enhance cold hardiness while maintaining fruit quality.In this study,we used‘Ecolly’as an intermediary grape variety for crossing with‘Cabernet Sauvignon’,‘Marselan’,and‘Dunkelfelder’,including three reciprocal crosses and a total of 1,657 intraspecific hybrids.We characterized the cold hardiness of these intraspecific hybrids and analyzed the genetic aspects of cold hardiness,ultimately identifying excellent strains with cold hardiness.Parameters like mean high-temperature exotherm(mHTE),mean low-temperature exotherm(mLTE),bound/free water ratio,water loss ratio in vitro,frost damage grades,and overall performance displayed partially normal distributions.In intraspecific hybrids,there was a maternal advantage in traits related to bound/free water ratio and water loss ratio.Some hybrid populations exhibited values for mHTE,mLTE,and water loss ratio that were lower than the low parent's values,while bound/free water ratio showed values higher than the high parent's values.Among the 1,657 intraspecific hybrids,52 strains could bud under stress at-18℃,and seven of these strains excelled in three important cold hardiness measures.Our study revealed that cold hardiness in V.vinifera is influenced by multiple genes and is a quantitative trait.Intraspecific hybridization can produce a small number of superior strains with enhanced cold hardiness.
基金The TNAU Master’s student fellowship to the senior author。
文摘Background Hybrid cotton enjoys overwhelming patronage among cotton farmers because of its superior yield capacity and fiber quality.However,various environmental factors affect its yield and fiber quality.This study aimed to assess 30 cotton hybrids for the stability of four traits(single-plant seed cotton yield,fiber upper half mean length(UHML),fiber strength,and micronaire)across three environments.Recent techniques,including genotype and genotype×environment(GGE)biplot,which provides a visual representation of performance and adaptability;weighted average absolute scores of the best linear unbiased predictions(WAASB),which balances the performance of the trait with stability;and multi-trait stability index(MTSI),which integrates multi-trait performance and stability,were used to analyze the stability of the four traits.Results Analysis of variance revealed significant genotype and environment interactions for all the traits studied,highlighting the need for comprehensive stability analysis.The environment E2 was the most suitable for the evaluation of seed cotton yield,whereas E3 was suitable for the evaluation of UHML and fiber strength.A stable hybrid,H05(TVH002×MCU5),with superior performance for seed cotton yield and UHML,was identified based on the overall results from GGE and WAASB.The which-won-where bioplot showed that H25(SVPR3×MCU5)performed the best for seed cotton yield in E3,and H27(Suraj×Sunantha)in E2 and E1.The hybrid H04(TVH002×CO14)in E1 and H30(Suraj×MCU5)in E2 and E3 performed well for UHML.Similarly,H28(Suraj×Suraksha)for E2 and E3 and H26(Suraj×Subiksha)for E1 were the best performing in the case of fiber strength.Based on the MTSI,four promising hybrids,namely,H24(SVPR3×CO14),H09(TVH2010×CO14),H18(MCU7×Suraksha),and H29(Suraj×CO14),were identified as stable with average performance for all four traits.Conclusions The study identified a stable hybrid,H05(TVH002×MCU5),with superior performance for yield and UHML.The identified hybrids in this study hold significant potential for cultivation across Tamil Nadu,with a scope for further evaluation in diverse environments.
基金the financial supports from National Key R&D Program for Young Scientists of China(Grant No.2022YFC3080900)National Natural Science Foundation of China(Grant No.52374181)+1 种基金BIT Research and Innovation Promoting Project(Grant No.2024YCXZ017)supported by Science and Technology Innovation Program of Beijing institute of technology under Grant No.2022CX01025。
文摘In this study,an inverse design framework was established to find lightweight honeycomb structures(HCSs)with high impact resistance.The hybrid HCS,composed of re-entrant(RE)and elliptical annular re-entrant(EARE)honeycomb cells,was created by constructing arrangement matrices to achieve structural lightweight.The machine learning(ML)framework consisted of a neural network(NN)forward regression model for predicting impact resistance and a multi-objective optimization algorithm for generating high-performance designs.The surrogate of the local design space was initially realized by establishing the NN in the small sample dataset,and the active learning strategy was used to continuously extended the local optimal design until the model converged in the global space.The results indicated that the active learning strategy significantly improved the inference capability of the NN model in unknown design domains.By guiding the iteration direction of the optimization algorithm,lightweight designs with high impact resistance were identified.The energy absorption capacity of the optimal design reached 94.98%of the EARE honeycomb,while the initial peak stress and mass decreased by 28.85%and 19.91%,respectively.Furthermore,Shapley Additive Explanations(SHAP)for global explanation of the NN indicated a strong correlation between the arrangement mode of HCS and its impact resistance.By reducing the stiffness of the cells at the top boundary of the structure,the initial impact damage sustained by the structure can be significantly improved.Overall,this study proposed a general lightweight design method for array structures under impact loads,which is beneficial for the widespread application of honeycomb-based protective structures.
基金funded by the Institute of Smart Energy,Huaiyin Institute of Technology,under Grant No.HIT-ISE-2024-07.
文摘In order to address environmental pollution and resource depletion caused by traditional power generation,this paper proposes an adaptive iterative dynamic-balance optimization algorithm that integrates the Improved Dung Beetle Optimizer(IDBO)with VariationalMode Decomposition(VMD).The IDBO-VMD method is designed to enhance the accuracy and efficiency of wind-speed time-series decomposition and to effectively smooth photovoltaic power fluctuations.This study innovatively improves the traditional variational mode decomposition(VMD)algorithm,and significantly improves the accuracy and adaptive ability of signal decomposition by IDBO selfoptimization of key parameters K and a.On this basis,Fourier transform technology is used to define the boundary point between high frequency and low frequency signals,and a targeted energy distribution strategy is proposed:high frequency fluctuations are allocated to supercapacitors to quickly respond to transient power fluctuations;Lowfrequency components are distributed to lead-carbon batteries,optimizing long-term energy storage and scheduling efficiency.This strategy effectively improves the response speed and stability of the energy storage system.The experimental results demonstrate that the IDBO-VMD algorithm markedly outperforms traditional methods in both decomposition accuracy and computational efficiency.Specifically,it effectively reduces the charge–discharge frequency of the battery,prolongs battery life,and optimizes the operating ranges of the state-of-charge(SOC)for both leadcarbon batteries and supercapacitors.In addition,the energy management strategy based on the algorithm not only improves the overall energy utilization efficiency of the system,but also shows excellent performance in the dynamic management and intelligent scheduling of renewable energy generation.
基金financially supported by the National Natural Science Foundation of China(Nos.22272118,22172111,and 22309134)the Science and Technology Commission of Shanghai Municipality,China(Nos.22ZR1464100,20ZR1460300,and 19DZ2271500)+2 种基金the China Postdoctoral Science Foundation(2022M712402),the Shanghai Rising-Star Program(23YF1449200)the Zhejiang Provincial Science and Technology Project(2022C01182)the Fundamental Research Funds for the Central Universities(2023-3-YB-07)。
文摘Carbon superstructures with multiscale hierarchies and functional attributes represent an appealing cathode candidate for zinc hybrid capacitors,but their tailor-made design to optimize the capacitive activity remains a confusing topic.Here we develop a hydrogen-bond-oriented interfacial super-assembly strategy to custom-tailor nanosheet-intertwined spherical carbon superstructures(SCSs)for Zn-ion storage with double-high capacitive activity and durability.Tetrachlorobenzoquinone(H-bond acceptor)and dimethylbenzidine(H-bond donator)can interact to form organic nanosheet modules,which are sequentially assembled,orientally compacted and densified into well-orchestrated superstructures through multiple H-bonds(N-H···O).Featured with rich surface-active heterodiatomic motifs,more exposed nanoporous channels,and successive charge migration paths,SCSs cathode promises high accessibility of built-in zincophilic sites and rapid ion diffusion with low energy barriers(3.3Ωs-0.5).Consequently,the assembled Zn||SCSs capacitor harvests all-round improvement in Zn-ion storage metrics,including high energy density(166 Wh kg-1),high-rate performance(172 m Ah g^(-1)at 20 A g^(-1)),and long-lasting cycling lifespan(95.5%capacity retention after 500,000 cycles).An opposite chargecarrier storage mechanism is rationalized for SCSs cathode to maximize spatial capacitive charge storage,involving high-kinetics physical Zn^(2+)/CF_(3)SO_(3)-adsorption and chemical Zn^(2+)redox with carbonyl/pyridine groups.This work gives insights into H-bond-guided interfacial superassembly design of superstructural carbons toward advanced energy storage.
文摘Multi-label feature selection(MFS)is a crucial dimensionality reduction technique aimed at identifying informative features associated with multiple labels.However,traditional centralized methods face significant challenges in privacy-sensitive and distributed settings,often neglecting label dependencies and suffering from low computational efficiency.To address these issues,we introduce a novel framework,Fed-MFSDHBCPSO—federated MFS via dual-layer hybrid breeding cooperative particle swarm optimization algorithm with manifold and sparsity regularization(DHBCPSO-MSR).Leveraging the federated learning paradigm,Fed-MFSDHBCPSO allows clients to perform local feature selection(FS)using DHBCPSO-MSR.Locally selected feature subsets are encrypted with differential privacy(DP)and transmitted to a central server,where they are securely aggregated and refined through secure multi-party computation(SMPC)until global convergence is achieved.Within each client,DHBCPSO-MSR employs a dual-layer FS strategy.The inner layer constructs sample and label similarity graphs,generates Laplacian matrices to capture the manifold structure between samples and labels,and applies L2,1-norm regularization to sparsify the feature subset,yielding an optimized feature weight matrix.The outer layer uses a hybrid breeding cooperative particle swarm optimization algorithm to further refine the feature weight matrix and identify the optimal feature subset.The updated weight matrix is then fed back to the inner layer for further optimization.Comprehensive experiments on multiple real-world multi-label datasets demonstrate that Fed-MFSDHBCPSO consistently outperforms both centralized and federated baseline methods across several key evaluation metrics.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Grant Nos.XDB28000000 and XDB0460000)the Quantum Science and Technology-National Science and Technology Major Project (Grant No.2021ZD0302600)the National Key Research and Development Program of China(Grant No.2024YFA1409002)。
文摘The hybridization gap in strained-layer InAs/In_(x)Ga_(1−x) Sb quantum spin Hall insulators(QSHIs)is significantly enhanced compared to binary InAs/GaSb QSHI structures,where the typical indium composition,x,ranges between 0.2 and 0.4.This enhancement prompts a critical question:to what extent can quantum wells(QWs)be strained while still preserving the fundamental QSHI phase?In this study,we demonstrate the controlled molecular beam epitaxial growth of highly strained-layer QWs with an indium composition of x=0.5.These structures possess a substantial compressive strain within the In_(0.5)Ga_(0.5)Sb QW.Detailed crystal structure analyses confirm the exceptional quality of the resulting epitaxial films,indicating coherent lattice structures and the absence of visible dislocations.Transport measurements further reveal that the QSHI phase in InAs/In_(0.5)Ga_(0.5)Sb QWs is robust and protected by time-reversal symmetry.Notably,the edge states in these systems exhibit giant magnetoresistance when subjected to a modest perpendicular magnetic field.This behavior is in agreement with the𝑍2 topological property predicted by the Bernevig–Hughes–Zhang model,confirming the preservation of topologically protected edge transport in the presence of enhanced bulk strain.
基金supported by Science and Technology Project of the headquarters of the State Grid Corporation of China(No.5500-202324492A-3-2-ZN).
文摘To enhance power flow regulation in scenarios involving large-scale renewable energy transmission via high-voltage direct current(HVDC)links and multi-infeed DC systems in load-center regions,this paper proposes a hybrid modular multilevel converter–capacitor-commutated line-commutated converter(MMC-CLCC)HVDC transmission system and its corresponding control strategy.First,the system topology is constructed,and a submodule configuration method for the MMC—combining full-bridge submodules(FBSMs)and half-bridge submodules(HBSMs)—is proposed to enable direct power flow reversal.Second,a hierarchical control strategy is introduced,includingMMCvoltage control,CLCC current control,and a coordinationmechanism,along with the derivation of the hybrid system’s power flow reversal characteristics.Third,leveraging the CLCC’s fast current regulation and theMMC’s negative voltage control capability,a coordinated power flow reversal control strategy is developed.Finally,an 800 kV MMC-CLCC hybrid HVDC system is modeled in PSCAD/EMTDC to validate the power flow reversal performance under a high proportion of full-bridge submodule configuration.Results demonstrate that the proposed control strategy enables rapid(1-s transition)and smooth switching of bidirectional power flow without modifying the structure of primary equipment:the transient fluctuation ofDC voltage from the rated value(UdcN)to themaximumreverse voltage(-kUdcN)is less than 5%;the DC current strictly follows the preset characteristic curve with a deviation of≤3%;the active power reverses continuously,and the system maintains stable operation throughout the reversal process.
基金Supported by National Freshwater Fish Industrial Technology System ProjectKey Discipline Construction Project of Shanghai(Y1101)Key Project of Science Committee in Shanghai(06DJ14003)~~
文摘[ Objective] The aim of this study was to investigate the growth and morphological characteristics of juvenile hybrids of grass carp. [Method] Three inbred generations of YR （Yangtze River stock ♀×Yangtze River stock ♂ ）, ZR （Zhujiang River stock ♀ ×Zhujiang River stock♂ ） and hybrids F1 （ Yangtze River stock ♀ × Zhujiang River stock ♂ ） were established, and their growth or morphological data were also measured. [ Result] The orders of body weight, standard length and absolute growth rate were F1 〉 ZR 〉 YR. No significant difference was found in body weight or standard length of 50 day-old generations （ P〉0. 05）, while there was an extremely significantly difference in those of 170 day-old generations （ P 〈0.01 ）. However, the absolute growth rate of F1 was 20.00% and 50.00% higher than that of ZR and YR respectively, and no significant difference was found between F1 and ZR （ P 〉 0.05）, but significant difference between F1 and YR （ P 〈 0.05）. F1 showed a significant hybrid vigor with rate of 20.09%. There was no significant difference among three inbred generations in standard length/total length （ P 〉 0.05）, while significant difference in head length/total length, body height/standard length and body width/standard length （ P 〈 0.05）, which indicated that F1 had the characteristics of shorter head, higher and wider body. [ Conclusion]F1 has advantages in growth performance and morphological characteristics.
基金Supported by Excellent Team Training Program of Yunnan Academy of Agriculture Sciences(YAAS2014YY002)~~
文摘[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
基金Supported by Special Basic Research Fund for Central Public Research Institutes(0032011018)~~
文摘To understand the inter-hybridization between Cucumis ssp.plants,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but seeds from fruits V2（V129）×C.metuliferus were abortive.A few of seeds from the bottom of fruit V2（V129）×C.anguria were fertile.Sequence-related amplified polymorphism（SRAP）molecular markers were used to analyze the progenies of inter-specific hybridization between C.anguria and melon V129.One pair primer（E14/M2）was found effective in amplification on male parent characteristic bands from the hybrids,suggesting that some DNA exchange had happened between C.anguria and melon V129.This study provided data for analyzing the mechanism of inter-hybridization between Cucumis plants.
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.
基金The research is supported by grant from the National Natural Science Foundation of China.
文摘Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confirm the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis + A. deliciosa) and four clones (A. chinensis + A. kolomikta) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis + A. deliciosa) clone were deduced as octoploid (2n = 8x), and the (A. chinensis + A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n = 5x). The clones were confirmed as interspecific somatic hybrids in Actinidia.
文摘Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.
基金Supported by Earmarked Fund for Modern Rapeseed Research System of China(nycytx-00563)High-oil Hybrid Brassica napus Rapeseed Cultivation Technology Research Program of Guizhou Academy of Agricultural Sciences[ZX(2007)015]+3 种基金New High-yield Rapeseed Cultivar Yoyan 599 Cultivation and Promotion Program of Agriculture Department of Guizhou Province[(2009)007]Hybrid Rapeseed Cultivar Youyan 599 Promotion Program of Guizhou Academy of Agricultural Sciences[(2009)030]New High-oil Rapeseed Cultivar Sanbei 98 Cultivation and Promotion Program of Agriculture Department of Guizhou Province[(2010)3087]Earmarked Fund for Modern Rapeseed Research System of Guizhou Province(GZCYTX2013-0802)~~
文摘[Objective] This study aimed to analyze the influence of five cultivation factors： sowing date, plant density, nitrogen fertilizer amount, phosphate fertilizer amount and potash fertilizer amount on oil quality and stability of Brassica napus hybrids. [Method] Statistical analysis was carried out based on the data from previous studies and our work. [Result] Sowing date had no significant influence on oil quality and stability of B. napus hybrids. The plant density shared a positive correlation with 18-C fatty acid （FA） content, unsaturated fatty acid （UFA） content, total content of linoleic acid and linolenic acid, index of unsaturated fatty acid （IUFA）, so a large plant density can improve the oil quality （by increasing18-C FA content, UFA content, total content of linoleic acid and linolenic acid）, but reduce the oil stability （by increasing IUFA）. The amount of nitrogen fertilizer had no significant influence on the contents of 18-C FA and UFA, but significant positive correlations with the total content of linoleic acid and linolenic acid and IUFA. So, the total content of linoleic acid and linolenic acid can be reduced by increasing nitrogen amount, meanwhile the oil stability was also decreased. Phosphate fertilizer amount did not affect 18-C FA significantly, but was negatively correlated with the UFA content, total content of linoleic acid and linolenic acid, and IUFA. So, increasing the amount of applied phosphate fertilizer can reduce UFA content, total content of linoleic acid and linolenic acid, thereby improving oil stability. Potash fertilizer had no significant influence on the IUFA, a significant positive correlation with 18-C FA content, an insignificant positive correlation with the the UFA content, and a negative correlation with the total content of linoleic acid and linolenic acid. The oil content of rapeseed shared an extremely significant positive correlation with 18-C FA content and UFA content, an negative correlation with the total content of linoleic acid and linolenic acid, and no significant correlation with IUFA. [Conclusion] This study identified the effects of five main cultivation factors on oil quality and stability of B. napus hybrids, and the correlations of oil content with 18-C FA content, UFA content, total content of linoleic acid and linolenic acid, and IUFA.
文摘The combined lines having both phKL and Ph2-deficiency were obtained in the genetic background of common wheat (Triticum aestivum L.) landrace. These lines had normal fertility. In the wheat combined lines X Aegilops variabilis Eig. (or rye), a significant increase in the chiasmata of homoeologous pairing was shown by the phKL+Ph2(-) plants with respect to their phKL+Ph2 sibs, which indicates that Ph2-deficiency and phKL showed an additive effect on promoting pairing. The effects were shown in the increment of rod bivalents, ring bivalents and trivalents and reduction of univalents, of which, reduction of univalents was mainly due to the increment of rod bivalents. The combined lines are probably more desirable materials for alien gene transferring than phKL or Ph2(-) lines alone. In comparison with that of ph1b X Ae. variabilis (or rye), phKL+Ph2(-) X Ae. variabilis (or rye) show higher (or similar) numbers of rod bivalents, while the total chromosome pairing level significantly reduced that ascribed to the decrement in ring bivalents and multivalents. These results probably indicate the different genetic mechanisms for Ph1 and Ph2 or phKL on controlling homoeologous pairing.
文摘The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background.
基金Supported by Science and Technology Project of He'nan Province(072102150001)Science and Technology Project of Zhengzhou City(083SGYG24123-6)~~
文摘[Objective] This study aimed to investigate the reason for the different leaf colors of pomegranate hybrid progeny. [Method] Spectrophotometry was used to determine the contents of three pigments, including chlorophyll, carotenoid and anthocyanin, in the leaves of eight pomegranate individuals and their parents at growth stage. [Result] The results showed that during the early growth stage, the anthocyanin contents of four pomegranate individuals with red leaves were higher than the control, to be specific, the anthocyanin content of No.5 pomegranate individual reached the highest of 4.12 mg/g; the chlorophyll and carotenoid contents of four pomegranate individuals with yellow-green leaves showed consistent dynamic trends with the control, to be specific, the chlorophyll content and chlorophyll/carotenoid ratio of No.4 pomegranate individual reached the highest, while the chlorophyll a/carotenoid b ratio of No.3 pomegranate individual was the lowest. During vigorous growth stage, the contents of chlorophyll, carotenoid and anthocyanin of eight pomegranate individuals showed consistent dynamic trends, with no significant difference with the control. Analysis of variance indicated that there were significant differences in the contents of chlorophyll and carotenoid among different pomegranate individuals at different growth stages and in the content of anthocyanin among different pomegranate individuals, while no significant difference was observed in the chlorophyll/carotenoid ratio among different pomegranate individuals at different growth stages. [Conclusion] The differences in leaf colors among pomegranate hybrid progeny are caused by different contents and ratios of anthocyanin and chlorophyll at growth stage.
