Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica par...Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica particles employing covalent attachment by EDC/NHS chemistry and via electrostatic interaction.The resulting formulations were examined for the morphology(SEM),chemical composition(FTIR,XPS)as well as protein content.To facilitate the injectability and support the bone regeneration,developed particles were dispersed in biopolymeric sol composed of collagen,chitosan and lysinemodifed hyaluronic acid and crosslinked with genipin.By means of rheological study,the sol-gel in situ transition of obtained systems was verifed.It was found in vitro study that MG-63 cells cultured on the developed composites exhibit signifcantly higher alkaline phosphatase(ALP)activity,compared to the pristine hydrogel.Furthermore,the biomineralization ability in the simulated body fluid(SBF)model was also demonstrated.Our fndings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects,especially as a less-cost effective support/alternative for BMP-2 systems.展开更多
基金the financial support of National Science Centre,Poland,Grant 2016/21/D/ST5/01635。
文摘Novel bioactive injectable composites based on biopolymeric hydrogels reinforced with insulin-functionalized silica particles were synthesized.The insulin(INS)was immobilized on the surface of amine-modifed silica particles employing covalent attachment by EDC/NHS chemistry and via electrostatic interaction.The resulting formulations were examined for the morphology(SEM),chemical composition(FTIR,XPS)as well as protein content.To facilitate the injectability and support the bone regeneration,developed particles were dispersed in biopolymeric sol composed of collagen,chitosan and lysinemodifed hyaluronic acid and crosslinked with genipin.By means of rheological study,the sol-gel in situ transition of obtained systems was verifed.It was found in vitro study that MG-63 cells cultured on the developed composites exhibit signifcantly higher alkaline phosphatase(ALP)activity,compared to the pristine hydrogel.Furthermore,the biomineralization ability in the simulated body fluid(SBF)model was also demonstrated.Our fndings suggest that proposed herein novel hydrogel-based composites might be the promising formulation for regeneration of bone defects,especially as a less-cost effective support/alternative for BMP-2 systems.
