Neural cell senescence hinders spinal cord nerve function recovery,and existing therapies that target senescent cell clearance haven’t effectively addressed cellular senescence.In this study,injectable short fibers t...Neural cell senescence hinders spinal cord nerve function recovery,and existing therapies that target senescent cell clearance haven’t effectively addressed cellular senescence.In this study,injectable short fibers that accurately maintain genome homeostasis in real time were developed,which for the first time reversed neural cell senescence by blocking the excessive intervention of cell inspection points.First,the oxidization-sensitive hybrid liposomes were prepared by combining Bakuchiol(BAK),a natural plant extract with the ability of DNA protection,with the oxidization-sensitive phospholipid S-PC.Subsequently,the short fibers regulating the cell inspection points(ISN@n-BAK)were constructed by further complexing the oxidation-sensitive hybrid liposomes with short fibers throughπ–πconjugation and catechol groups mussel-stimulated polydopamine(PDA).In vitro experiments demonstrated that ISN@n-BAK promotes neural stem cell differentiation into neurons and has anti-aging effects across various aging stages.In vivo,ISN@n-BAK responded to excessive ROS by triggering oxidation-sensitive liposomes to release BAK,protecting against DNA damage,suppressing aging-related gene expression in Cdkn2a and Cdkn2c and inhibiting inspection point restrictions.Bioinformatics showed that ISN@n-BAK reversed neural cell senescence and aided spinal cord nerve regeneration by activating the endogenous cell cycle,downregulating the PI3K-Akt pathway and upregulating the Rap1 pathway.This study introduces a novel therapeutic approach using short fibers that inhibit inspection points intervention to rejuvenate injured spinal cords.展开更多
Subgrade in highway engineering construction is the most basic construction content. It can play the role of supporting pavement and control the construction quality of subgrade engineering. It can provide important g...Subgrade in highway engineering construction is the most basic construction content. It can play the role of supporting pavement and control the construction quality of subgrade engineering. It can provide important guarantee for the construction of highway structural layer and surface layer, ensure the quality of the constructed highway meets the requirements and improve the service life of highway engineering. Based on this, the article discusses the construction quality management and inspection points of subgrade engineering.展开更多
Steam turbine as one of the key equipment of metallurgical power system production, running safety and reliability of the whole power system has great significance, and in order to ensure the normal and stable operati...Steam turbine as one of the key equipment of metallurgical power system production, running safety and reliability of the whole power system has great significance, and in order to ensure the normal and stable operation of the steam turbine, maintenance personnel also need to conduct a comprehensive inspection and maintenance of the steam turbine itself, to better promote the production safety. This paper mainly provides a detailed overview of the steam turbine, to its maintenance and maintenance of the key technical points and related matters needing attention for the in-depth discussion, but also hopes to make a certain reference value for the relevant personnel.展开更多
基金supported by the National Nature Science Foundation of China(32000937,81871549 and 81971312)Science and Technology Commission of Shanghai Municipality(23015820800)Shanghai Municipal Health Commission(20204Y0355).
文摘Neural cell senescence hinders spinal cord nerve function recovery,and existing therapies that target senescent cell clearance haven’t effectively addressed cellular senescence.In this study,injectable short fibers that accurately maintain genome homeostasis in real time were developed,which for the first time reversed neural cell senescence by blocking the excessive intervention of cell inspection points.First,the oxidization-sensitive hybrid liposomes were prepared by combining Bakuchiol(BAK),a natural plant extract with the ability of DNA protection,with the oxidization-sensitive phospholipid S-PC.Subsequently,the short fibers regulating the cell inspection points(ISN@n-BAK)were constructed by further complexing the oxidation-sensitive hybrid liposomes with short fibers throughπ–πconjugation and catechol groups mussel-stimulated polydopamine(PDA).In vitro experiments demonstrated that ISN@n-BAK promotes neural stem cell differentiation into neurons and has anti-aging effects across various aging stages.In vivo,ISN@n-BAK responded to excessive ROS by triggering oxidation-sensitive liposomes to release BAK,protecting against DNA damage,suppressing aging-related gene expression in Cdkn2a and Cdkn2c and inhibiting inspection point restrictions.Bioinformatics showed that ISN@n-BAK reversed neural cell senescence and aided spinal cord nerve regeneration by activating the endogenous cell cycle,downregulating the PI3K-Akt pathway and upregulating the Rap1 pathway.This study introduces a novel therapeutic approach using short fibers that inhibit inspection points intervention to rejuvenate injured spinal cords.
文摘Subgrade in highway engineering construction is the most basic construction content. It can play the role of supporting pavement and control the construction quality of subgrade engineering. It can provide important guarantee for the construction of highway structural layer and surface layer, ensure the quality of the constructed highway meets the requirements and improve the service life of highway engineering. Based on this, the article discusses the construction quality management and inspection points of subgrade engineering.
文摘Steam turbine as one of the key equipment of metallurgical power system production, running safety and reliability of the whole power system has great significance, and in order to ensure the normal and stable operation of the steam turbine, maintenance personnel also need to conduct a comprehensive inspection and maintenance of the steam turbine itself, to better promote the production safety. This paper mainly provides a detailed overview of the steam turbine, to its maintenance and maintenance of the key technical points and related matters needing attention for the in-depth discussion, but also hopes to make a certain reference value for the relevant personnel.
