Cotton bollworm(Helicoverpa armigera) is an important agricultural pest that causes severe yield loss to crops,particularly to cotton.Transgenic Bt crops have been successful in
Numerous studies indicate that target gene silencing by RNA interference （RNAi） could lead to insect death. This phenomenon has been considered as a potential strategy for insect pest control, and it is termed RNAi-...Numerous studies indicate that target gene silencing by RNA interference （RNAi） could lead to insect death. This phenomenon has been considered as a potential strategy for insect pest control, and it is termed RNAi-mediated crop protection. However, there are many limitations using RNAi-based technology for pest control, with the effectiveness target gene selection and reliable double-strand RNA （dsRNA） delivery being two of the major challenges. With respect to target gene selection, at present, the use of homologous genes and genome-scale high-throughput screening are the main strategies adopted by researchers. Once the target gene is identified, dsRNA can be delivered by micro-injection or by feeding as a dietary component. However, micro-injection, which is the most common method, can only be used in laboratory experiments. Expression of dsRNAs directed against insect genes in transgenic plants and spraying dsRNA reagents have been shown to induce RNAi effects on target insects. Hence, RNAi-mediated crop protection has been considered as a potential new-generation technology for pest control, or as a complementary method of existing pest control strategies; however, further devel- opment to improve the efficacy of protection and range of species affected is necessary. In this review, we have summarized current research on RNAi-based technology for pest insect management. Current progress has proven that RNAi technology has the potential to be a tool for designing a new generation of insect control measures. To accelerate its practical application in crop protection, further study on dsRNA uptake mechanisms based on the knowledge of insect physiology and biochemistry is needed.展开更多
The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of ...The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of the residues of chemical insecticides in alfalfa and their influence on the quality of alfalfa hay were examined.Efficacy varied among the different biological and chemical insecticides.The chemical insecticides were significantly more effective than biopesticides in a short time-frame.The efficacy period of biopesticides was significantly longer than that of chemical insecticides,and the corrected mortality rate of aphids in all regions was above 50%at 14 days after application.The analysis of pesticide residues showed that the residual doses of all the pesticides were within the allowed ranges after the safe period.The acid detergent fiber and neutral detergent fiber contents in alfalfa hay were higher and the protein content was lower in chemical insecticide treatments than in biopesticide treatments in Hebei.The relative feeding value of alfalfa hay treated with Metarhizium anisopliae IPP330189 was the highest among the treatments.Compared with chemical insecticides,the yield of alfalfa hay was higher in the biopesticides treatments.Biopesticides show a stronger control effect on insect populations and also a better improvement in the quality of alfalfa hay than chemical insecticides.This study provides a basis for exploring and developing a comprehensive control regime for alfalfa insect pests in the different alfalfa-growing regions in China,and for reducing chemical insecticide usage and improving forage quality.展开更多
Biological control through habitat management leads to sustainable insect pest control. Different types of land composition such as multiple landscapes, patchiness of landscapes enhance the natural enemies which ultim...Biological control through habitat management leads to sustainable insect pest control. Different types of land composition such as multiple landscapes, patchiness of landscapes enhance the natural enemies which ultimately lead to control of insect pest. Plant characteristics such as flower shape, flower color and blooming period ensures excess food for natural enemies like nectar and pollen. Moreover, some agricultural practices such as tillage, crop rotation, and intercropping influence the natural enemies especially parasitoid and preda-tors. Consequently, they increase longevity and fecundity of parasitoid and predator that help to control insect pest. One of the most important recently used methods is push-pull which consists of semiochemicals called Herbi-vore-Induced Plant Volatiles (HIPVs). This compound has been used to pre-vent pest and attract natural enemies.展开更多
Soybean rust,soybean downy mildew,and soybean thrips,soybean pod borers,and soybean nocturnal moths are the world wide diseases and insect pests in soybean production,which pose a potential threat to soybean productio...Soybean rust,soybean downy mildew,and soybean thrips,soybean pod borers,and soybean nocturnal moths are the world wide diseases and insect pests in soybean production,which pose a potential threat to soybean production in Great Mekong Sub-region（ GMS）,comprising Cambodia,Lao People＇s Democratic Republic,Myanmar,Thailand,Vietnam,and Yunnan province,the People＇s Republic of China. This paper summarized the host range,epidemiology,damage and control methods of these diseases and insect pests in GMS,with the aim to provide information basis for understanding and effective control of soybean diseases and insect pests in GMS.展开更多
[ Objective] The paper was to study control effects of 12 kinds of insecticides on sugarcane pests at different periods. [ Method] By field experiment, the control effects of different insecticides against sugarcane p...[ Objective] The paper was to study control effects of 12 kinds of insecticides on sugarcane pests at different periods. [ Method] By field experiment, the control effects of different insecticides against sugarcane pests at planting stage, seedling stage and tillering stage of sugarcane were studied. [ Result] After seedcane was treated by Chlorantraniliprole, Durivo and Seed soaking, the dead heart seedlings were significantly reduced in fields, and the control effects were 87.13% ± 12.87%, 85.22% ± 14.78%, 82.71% ± 17.29%, respectively ; Durivo （ seedling emergence rate is 63.00% ± 10.30% ） and Seed soaking （ seedling emergence rate is 57.75 % ± 4.50% ） could also significantly enhance seedling emergence rate. After ＂removal-irrigation＂ treatment during seedling stage, the con- tml effects of Flubendiamide （76.26% ±4.31% ） and Chlorantraniliprele （61.66% ±6.65% ） against sugarcane stem borer were significantly higher than Virtako （60.17% ±7.06%）, Deltamethrin（38.75% ±9.88%）, lambda-cyhalothrin （54.06% ±6.83%）, 18% Dimehypo AS （37.98% ±10.31%） and Dipterex （38.64% ±9.34% ）. During tillering stage, with the increased application of 3.6% Dimehypo GR in cultivation and earthing up, the control effect on stem borer was significantly increased, and the control effect reached （72.03%± 17.06% ） as the dosage was 120 kg/hm2 ; when the dosage of phoxim exceeded 30 kg/hm2, the control effect on underground pests reached 44.00% ± 14.80%, and the control effect did not have significant difference as the dosage was ranged from 30 kg/hm2 to 60 kg/hm2. [ Conclusion] The paper provided scientific basis for selection of different control methods, different chemicals and dosages against pests during different periods.展开更多
The United States, Brazil, Argentina, India and China are the major soybean producing countries in the world. Nearly 90% of the world^s soybean production comes from these countries. The occurrence of diseases and i...The United States, Brazil, Argentina, India and China are the major soybean producing countries in the world. Nearly 90% of the world^s soybean production comes from these countries. The occurrence of diseases and insect pests often lead to the reduction of soybean yield, and brings varying degree losses to these countries. This article provides an overview of the impact and measures on soybean main diseases and insect pests in the top five major soybean producing countries over the world. It is concluded that the diseases affecting the soybean yield seriously include Phakopsorapachyrhizi, Heterodera glycines, Septoria glycines, Colletotrichum spp. and Macrophominaphaseolina; and the main insect pests include Anticarsia gemmatalis, Spodoptera litura, Nezara viridula and Frankliniella occidentalis, which will provide information for key prevention and control of soybean main diseases and insect pests in these countries.展开更多
This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part...This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part of the tree to kill pests. The change of pesticides inside the tree was observed. The result showed that injecting pesticides once a year could kill above 80% of the pests which happened once annually. The effect of injection lasts longer than that of applying the pesticide outside. The pesticides injected into the tree are not affected by environment and do not kill natural enemies directly,and the residual amount of pesticides is below the international standard level.展开更多
Jujube is a very common cash crop in our country. The requirement of geological environment is very low during the cultivation of jujube, so neither climate nor temperature will affect the cultivation of jujube. The t...Jujube is a very common cash crop in our country. The requirement of geological environment is very low during the cultivation of jujube, so neither climate nor temperature will affect the cultivation of jujube. The taste of jujube is sweet, and jujube is rich in nutrient elements, so it is loved by many people. In addition, jujube also contains some trace elements, so it also has certain medicinal value.展开更多
Cotton leaf worm, Spodoptera littoralis, is considered one of the most common arthropod pests that contribute to crop devastation of cotton. Previously, agricultural pests were controlled through the application of ch...Cotton leaf worm, Spodoptera littoralis, is considered one of the most common arthropod pests that contribute to crop devastation of cotton. Previously, agricultural pests were controlled through the application of chemical insecticides. However, insecticide-resistant insect populations emergence, as well as increasing concerns about the environmental and human health risks. Venomous animals introduce valuable sources of bioactive compounds which are employed for defence. Some of these toxins have high phyletic specificity, making them appropriate for use in IPM programs. This study aims to test the insecticidal effects of Polistes dominulus and Apis mellifera venoms. Crude venoms were examined for their insecticidal effect against cotton leaf worms by four different application methods including: leaf dipping, integument dropping, spraying, and injection. The data demonstrated a strong response to purified (Polistes dominulus) venom at an initial time than that of honey bee (Apis mellifera) which increases response with increasing the dose and the time interval. A dosage of 0.015 - 0.16 ml of both venoms had notably varied in LD<sub>50</sub> values on Spodoptera littoralis that showed higher wasp venom toxicity. Cotton leaf worms showed more susceptibility and mortality to the Polistes sp. venom than that of honey bees.展开更多
文摘Cotton bollworm(Helicoverpa armigera) is an important agricultural pest that causes severe yield loss to crops,particularly to cotton.Transgenic Bt crops have been successful in
文摘Numerous studies indicate that target gene silencing by RNA interference （RNAi） could lead to insect death. This phenomenon has been considered as a potential strategy for insect pest control, and it is termed RNAi-mediated crop protection. However, there are many limitations using RNAi-based technology for pest control, with the effectiveness target gene selection and reliable double-strand RNA （dsRNA） delivery being two of the major challenges. With respect to target gene selection, at present, the use of homologous genes and genome-scale high-throughput screening are the main strategies adopted by researchers. Once the target gene is identified, dsRNA can be delivered by micro-injection or by feeding as a dietary component. However, micro-injection, which is the most common method, can only be used in laboratory experiments. Expression of dsRNAs directed against insect genes in transgenic plants and spraying dsRNA reagents have been shown to induce RNAi effects on target insects. Hence, RNAi-mediated crop protection has been considered as a potential new-generation technology for pest control, or as a complementary method of existing pest control strategies; however, further devel- opment to improve the efficacy of protection and range of species affected is necessary. In this review, we have summarized current research on RNAi-based technology for pest insect management. Current progress has proven that RNAi technology has the potential to be a tool for designing a new generation of insect control measures. To accelerate its practical application in crop protection, further study on dsRNA uptake mechanisms based on the knowledge of insect physiology and biochemistry is needed.
基金supported by China Agriculture Research System of MOF and MARA(CARS-34).
文摘The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of the residues of chemical insecticides in alfalfa and their influence on the quality of alfalfa hay were examined.Efficacy varied among the different biological and chemical insecticides.The chemical insecticides were significantly more effective than biopesticides in a short time-frame.The efficacy period of biopesticides was significantly longer than that of chemical insecticides,and the corrected mortality rate of aphids in all regions was above 50%at 14 days after application.The analysis of pesticide residues showed that the residual doses of all the pesticides were within the allowed ranges after the safe period.The acid detergent fiber and neutral detergent fiber contents in alfalfa hay were higher and the protein content was lower in chemical insecticide treatments than in biopesticide treatments in Hebei.The relative feeding value of alfalfa hay treated with Metarhizium anisopliae IPP330189 was the highest among the treatments.Compared with chemical insecticides,the yield of alfalfa hay was higher in the biopesticides treatments.Biopesticides show a stronger control effect on insect populations and also a better improvement in the quality of alfalfa hay than chemical insecticides.This study provides a basis for exploring and developing a comprehensive control regime for alfalfa insect pests in the different alfalfa-growing regions in China,and for reducing chemical insecticide usage and improving forage quality.
文摘Biological control through habitat management leads to sustainable insect pest control. Different types of land composition such as multiple landscapes, patchiness of landscapes enhance the natural enemies which ultimately lead to control of insect pest. Plant characteristics such as flower shape, flower color and blooming period ensures excess food for natural enemies like nectar and pollen. Moreover, some agricultural practices such as tillage, crop rotation, and intercropping influence the natural enemies especially parasitoid and preda-tors. Consequently, they increase longevity and fecundity of parasitoid and predator that help to control insect pest. One of the most important recently used methods is push-pull which consists of semiochemicals called Herbi-vore-Induced Plant Volatiles (HIPVs). This compound has been used to pre-vent pest and attract natural enemies.
基金Supported by the Natural Science Fund for the Youth of Yunnan Agricultural University(2016ZR18)the Project of Key Laboratory of Integrated Pest Management on Crops in South China,Ministry of Agriculture,P.R.China(SCIPM2018-08)the Key Project of Agricultural Entomology and Pest Control in Yunnan Agricultural University(A2001206)
文摘Soybean rust,soybean downy mildew,and soybean thrips,soybean pod borers,and soybean nocturnal moths are the world wide diseases and insect pests in soybean production,which pose a potential threat to soybean production in Great Mekong Sub-region（ GMS）,comprising Cambodia,Lao People＇s Democratic Republic,Myanmar,Thailand,Vietnam,and Yunnan province,the People＇s Republic of China. This paper summarized the host range,epidemiology,damage and control methods of these diseases and insect pests in GMS,with the aim to provide information basis for understanding and effective control of soybean diseases and insect pests in GMS.
基金Supported by Basal Research Fund of Central Public-interest Scientific Institution(ITBB110503)Special Fund for Construction of Modern AgriculturalTechnology System(CARS-20-2-5)Sub-topics of Hainan Major Scientificand Technological Project:Germplasm and Genetic Resources of Tropical Organism(ZDZX2013023-1)
文摘[ Objective] The paper was to study control effects of 12 kinds of insecticides on sugarcane pests at different periods. [ Method] By field experiment, the control effects of different insecticides against sugarcane pests at planting stage, seedling stage and tillering stage of sugarcane were studied. [ Result] After seedcane was treated by Chlorantraniliprole, Durivo and Seed soaking, the dead heart seedlings were significantly reduced in fields, and the control effects were 87.13% ± 12.87%, 85.22% ± 14.78%, 82.71% ± 17.29%, respectively ; Durivo （ seedling emergence rate is 63.00% ± 10.30% ） and Seed soaking （ seedling emergence rate is 57.75 % ± 4.50% ） could also significantly enhance seedling emergence rate. After ＂removal-irrigation＂ treatment during seedling stage, the con- tml effects of Flubendiamide （76.26% ±4.31% ） and Chlorantraniliprele （61.66% ±6.65% ） against sugarcane stem borer were significantly higher than Virtako （60.17% ±7.06%）, Deltamethrin（38.75% ±9.88%）, lambda-cyhalothrin （54.06% ±6.83%）, 18% Dimehypo AS （37.98% ±10.31%） and Dipterex （38.64% ±9.34% ）. During tillering stage, with the increased application of 3.6% Dimehypo GR in cultivation and earthing up, the control effect on stem borer was significantly increased, and the control effect reached （72.03%± 17.06% ） as the dosage was 120 kg/hm2 ; when the dosage of phoxim exceeded 30 kg/hm2, the control effect on underground pests reached 44.00% ± 14.80%, and the control effect did not have significant difference as the dosage was ranged from 30 kg/hm2 to 60 kg/hm2. [ Conclusion] The paper provided scientific basis for selection of different control methods, different chemicals and dosages against pests during different periods.
基金Supported by Fund Project of Key Laboratory of Integrated Pest Management on Crops in South China,Ministry of Agriculture,P.R.China(SCIPM2018-08)Natural Science Youth Fund of Yunnan Agricultural University(2016ZR18)Key Discipline Project of Agricultural Entomology and Pest Control in Yunnan Agricultural University(A2001206)
文摘The United States, Brazil, Argentina, India and China are the major soybean producing countries in the world. Nearly 90% of the world^s soybean production comes from these countries. The occurrence of diseases and insect pests often lead to the reduction of soybean yield, and brings varying degree losses to these countries. This article provides an overview of the impact and measures on soybean main diseases and insect pests in the top five major soybean producing countries over the world. It is concluded that the diseases affecting the soybean yield seriously include Phakopsorapachyrhizi, Heterodera glycines, Septoria glycines, Colletotrichum spp. and Macrophominaphaseolina; and the main insect pests include Anticarsia gemmatalis, Spodoptera litura, Nezara viridula and Frankliniella occidentalis, which will provide information for key prevention and control of soybean main diseases and insect pests in these countries.
文摘This research is to control the pests inside fruits and buds by injecting pesticides into the tree with the high-pressure injector. The soluable pesticides injected into the trunk can transported insides to every part of the tree to kill pests. The change of pesticides inside the tree was observed. The result showed that injecting pesticides once a year could kill above 80% of the pests which happened once annually. The effect of injection lasts longer than that of applying the pesticide outside. The pesticides injected into the tree are not affected by environment and do not kill natural enemies directly,and the residual amount of pesticides is below the international standard level.
文摘Jujube is a very common cash crop in our country. The requirement of geological environment is very low during the cultivation of jujube, so neither climate nor temperature will affect the cultivation of jujube. The taste of jujube is sweet, and jujube is rich in nutrient elements, so it is loved by many people. In addition, jujube also contains some trace elements, so it also has certain medicinal value.
文摘Cotton leaf worm, Spodoptera littoralis, is considered one of the most common arthropod pests that contribute to crop devastation of cotton. Previously, agricultural pests were controlled through the application of chemical insecticides. However, insecticide-resistant insect populations emergence, as well as increasing concerns about the environmental and human health risks. Venomous animals introduce valuable sources of bioactive compounds which are employed for defence. Some of these toxins have high phyletic specificity, making them appropriate for use in IPM programs. This study aims to test the insecticidal effects of Polistes dominulus and Apis mellifera venoms. Crude venoms were examined for their insecticidal effect against cotton leaf worms by four different application methods including: leaf dipping, integument dropping, spraying, and injection. The data demonstrated a strong response to purified (Polistes dominulus) venom at an initial time than that of honey bee (Apis mellifera) which increases response with increasing the dose and the time interval. A dosage of 0.015 - 0.16 ml of both venoms had notably varied in LD<sub>50</sub> values on Spodoptera littoralis that showed higher wasp venom toxicity. Cotton leaf worms showed more susceptibility and mortality to the Polistes sp. venom than that of honey bees.
