[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse condition...[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.展开更多
[Objective] The study aimed to study the antifungal activity of biocontrol strains against Alternaria mali,colonization ability in apple leaves and the impact of some chemical fungicides on biocontrol strains were als...[Objective] The study aimed to study the antifungal activity of biocontrol strains against Alternaria mali,colonization ability in apple leaves and the impact of some chemical fungicides on biocontrol strains were also studied.[Method] Healthy leaves of Matsumotokin were collected to conduct vitro leaf test,colonization test and influence test of different fungicides on biocontrol strains.[Result] Vitro leaf test showed that B.subtilis BS-315 had strong inhibition effect on A.mali,when biocontrol strain was daubed on leaves,and pathogen was inoculated after 1 d,the inhibition effect of BS-315 against A.mali was over 90% after 3 d.BS-315 could colonize in leaves of Matsumotokin,colonization volume reached the maximum after inoculation for 3 d.Azoxystrobin and polyoxin had certain promoting effect on the growth of BS-315,while carbendazim and propiconazole completely inhibited the growth of BS-315.[Conclusion] This study would provide theoretical basis for development and rational utilization of biocontrol preparations.展开更多
The inhibition effect of rare earths on the production of ·O - 2 produced by radiolysis of aqueous sodium formate saturated with O 2 with high energy pulse electron beam was studied. The result indicates ...The inhibition effect of rare earths on the production of ·O - 2 produced by radiolysis of aqueous sodium formate saturated with O 2 with high energy pulse electron beam was studied. The result indicates that rare earth nitrate obviously inhibits the production of ·O - 2 . The inhibition rate is between 28 6% and 92%. The inhibition effect increases with the increase of rare earth nitrates concentration. The distinguish dose effect relationship was observed.展开更多
The phenyl and methyl phenyl free radicals,formed in the oxidation-hydrolysis process of N’-acyl-N-phenylhydrazine, have been studied by ESR spectrometry.It was found that the free radical is the chief cause which pr...The phenyl and methyl phenyl free radicals,formed in the oxidation-hydrolysis process of N’-acyl-N-phenylhydrazine, have been studied by ESR spectrometry.It was found that the free radical is the chief cause which produces the feed-back inhibition effect in the hydrazine-promoted infectious development of silver halide.展开更多
In controlling eutrophication phenomenon, there were conventional methods which lead to negative effects to aquatic environment. This study was aimed to investigate the usage of synthesized thiourea compounds to inhib...In controlling eutrophication phenomenon, there were conventional methods which lead to negative effects to aquatic environment. This study was aimed to investigate the usage of synthesized thiourea compounds to inhibit the growth of Oscillatoria sp. in Kenyir lake, Terengganu, Malaysia. The inhibition effects of four eco-friendly alkoxyl thiourea derivatives compounds named as N-（（4-（decyloxy）phenyl）carbamothioyl）-4-methyl benzamide, N-（（4-（decyloxy）phenyl）carbamothioyl）-4-nitro benzamide, 4-chloro-N-（（4-（decyloxy）phenyl）carbamothioyl） benzamide and N-（（4-（decyloxy）phenyl）carbamothioyl） benzamide were examined onto the growth culture of Oscillatoria sp. These compounds were tested in 30 mL of Oscillatoria sp. cultures with different concentration of 16 μg·mL-1, 18 μg·mL-1, 20 μg·mL-1, 24 μg·mL-1 and 28 μg·mL-1respectively. The treatment flasks were supplied with an aerator for 24 hours under continuous illumination at 25 ℃. Chlorophyll-a concentration were extracted to calculate the inhibition percentage of each treatment. Overall, all these compounds showed inhibition effects towards the growth of Oscillatoria sp., with the highest inhibition of 37% by N-（（4-（decyloxy）phenyl）carbamothioyl）-4-methyl benzamide at the concentration of 18μg·mL-1. The methyl group that attach to the synthesized compound may contribute to the effectiveness of the compound which act as an algae inhibitor. However, extensive studies still need to be conducted in order to investigate the mechanism on how this compound reacts with Oscillatoria sp..展开更多
Objective To determine the effect of the phosphodiesterase-5 inhibitor sildenafil compared with placebo on exercise capacity and clinical status in HFPEF. Design Multicenter, double-blind, placebo-controlled, paralle...Objective To determine the effect of the phosphodiesterase-5 inhibitor sildenafil compared with placebo on exercise capacity and clinical status in HFPEF. Design Multicenter, double-blind, placebo-controlled, parallel-group, randomized clinical trial of 216 sta- ble outpatients with HF, ejection fraction ≥ 50%, elevated N-terminal brain-type natriuretic peptide or elevat- ed invasively measured filling pressures, and reduced exercise capacity. Participants were randomized from October 2008 through February 2012 at 26 centers in North America. Follow-up was through August 30, 2012.展开更多
ABSTRACT The intensity of antiplatelet therapy during percutaneous coronary intervention （PCI） is an important deter- minant of PCI-related ischemic complications. Cangrelor is a potent intravenous adenosine diphos...ABSTRACT The intensity of antiplatelet therapy during percutaneous coronary intervention （PCI） is an important deter- minant of PCI-related ischemic complications. Cangrelor is a potent intravenous adenosine diphosphate （ADP）- receptor antagonist that acts rapidly and has quickly reversible effects.展开更多
Bensulfuron-Methyl(BSM)is a highly active sul-fonylurea herbicide that effectively controls most an-nual and perennial broadleaf or sedge weeds in paddyfield.However,the activity of BSM is affected byseveral evironmen...Bensulfuron-Methyl(BSM)is a highly active sul-fonylurea herbicide that effectively controls most an-nual and perennial broadleaf or sedge weeds in paddyfield.However,the activity of BSM is affected byseveral evironmental factors.Using corn seedlings asmaterial,we found that under different temperatures展开更多
The stability of oil-dominated emulsions,including oil-based drilling fluids and crude oils,is crucial for mitigating gas hydrate risks in the petroleum and natural gas industries.Nanoparticles can stabilize oilwater ...The stability of oil-dominated emulsions,including oil-based drilling fluids and crude oils,is crucial for mitigating gas hydrate risks in the petroleum and natural gas industries.Nanoparticles can stabilize oilwater systems(Pickering emulsions)by residing at the oil-water interface.However,their effects on the kinetics of hydrate formation in these systems remain unclear.To address this,we experimentally investigated how hydrophilic and hydrophobic nano-CaCO_(3) influence CH4 hydrate formation within dynamic oil-water systems.A series of hydrate formation experiments were conducted with varying water cuts and different concentrations of nano-CaCO_(3) at a particle size of 20 nm,under 3℃ and 6 MPa.The induction time,hydrate formation volume,and hydrate growth rate were measured and calculated.The results indicate that hydrophilic nano-CaCO_(3) generally inhibits hydrate formation,particularly at high water cuts,while hydrophobic nano-CaCO_(3) can significantly inhibit or even prevent hydrate formation at low water cuts.Water cut strongly influences the kinetics of hydrate formation,and nanoparticle concentration also impacts the results,likely due to changes in oil-water interface stability caused by nanoparticle distribution.This study will offer valuable insights for designing deepwater oilbased drilling fluids using nanoparticles and ensuring safe multiphase flow in deepwater oil and gas operations.展开更多
In this work,a series of molybdovanadophosphoric heteropoly acid quaternary ammonium salts H_（3＋x）PMo_（12 -x）V_xO40-T were synthesized and employed as a reaction inhibitor in the selfpolymerization of methyl meth...In this work,a series of molybdovanadophosphoric heteropoly acid quaternary ammonium salts H_（3＋x）PMo_（12 -x）V_xO40-T were synthesized and employed as a reaction inhibitor in the selfpolymerization of methyl methacrylate（MMA）.The polymerization inhibition effect of H_（3＋x）PMoPMo_（12 -x）V_xO40-T）with different number of vanadium atoms and reaction dosages was investigated using differential scanning calorimetry（DSC）.It shows that the inhibitory effect was improved with the increasing dosages of H_（3＋x）PMoPMo_（12 -x）V_xO40-T）,and the polymerization inhibition was also affected by the number of vanadium atoms in the H_（3＋x）PMo_（12 -x）V_xO40-T .Furthermore,cyclic voltammograms（CV）was used to probe the mechanism of the inhibition reaction with H3＋xPMo12xVxO40-T.The result of CV indicates that the inhibition reaction is an oxidation–reduction reaction.H_（3＋x）PMo_（12 -x）V_xO40-T can react directly with the MMA monomer radicals,which eliminated the MMA monomers,and therefore the self-polymerization of the MMA can be effectively inhibited by H_（3＋x）PMo_（12 -x）V_xO40-T.展开更多
The synergistic inhibition effect of CeCl3(Ce)and serine(Ser)on the corrosion of carbon steel in a 3%NaCl solution was investigated by electrochemical methods and surface analysis.The results showed that both CeCl3 an...The synergistic inhibition effect of CeCl3(Ce)and serine(Ser)on the corrosion of carbon steel in a 3%NaCl solution was investigated by electrochemical methods and surface analysis.The results showed that both CeCl3 and Ser,when used alone,had limited inhibition effect toward carbon steel corrosion in the 3%NaCl solution.In contrast,the combination of CeCl3 with Ser produced a strong synergistic effect on the corrosion inhibition behavior of carbon steel,improving the inhibition efficiency significantly.The polarization curves showed that the mixture of CeCl3 and Ser acts as a cationic-type inhibitor.Scanning electron microscopy and Fourier transform infrared spectroscopy showed that the synergistic inhibition effect was due to complex formation between the cerium ions and amino acid molecules.展开更多
[Objectives] To explore the inhibitory effect of octadecadienoic acid (ODA) on proliferation and apoptosis of glioma cells and its mechanism. [Methods] Cultured human glioma cells (cell density 2×10^(6) cells/L) ...[Objectives] To explore the inhibitory effect of octadecadienoic acid (ODA) on proliferation and apoptosis of glioma cells and its mechanism. [Methods] Cultured human glioma cells (cell density 2×10^(6) cells/L) were divided into three groups: solvent control group (DMSO, 30 μL/L), 5-FU group (10 mg/L) and octadecadienoic acid group (0.3, 0.6, 1.2 mg/L). The toxic effects of ODA on glioma cells were detected by trypan blue and thiazolium blue (MTT). The expression of P53, PI3K, P21, PKB/Akt and caspase-9 protein in glioma cells were detected by enzyme-linked immunosorbent assay (ELISA). [Results] The cell count under optical microscope showed that the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group was significantly higher than that in solvent control group ( P <0.01), but there was no significant difference compared with 5-FU group ( P >0.05). The results of MTT showed that compared with the solvent control group, the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group significantly increased ( P <0.01);compared with 5-FU group, the inhibition rate of cell proliferation in high dose ODA group significantly increased ( P <0.01). The results of flow cytometry showed that compared with the solvent control group, the number of cells in G_(0)/G_(1) phase increased significantly ( P <0.05, P <0.01), the number of cells in G_(2)/M phase decreased significantly ( P <0.01) and the apoptosis rate increased significantly ( P <0.01) in the low, medium and high dose ODA groups and 5-FU group;compared with 5-FU group, the number of cells in G_(2)/M phase decreased significantly ( P <0.01) and the apoptosis rate increased significantly ( P <0.01) in ODA group. ELISA testing results showed that the expression levels of P53, P13K and PKB/Akt in low, medium and high dose ODA groups and 5-FU group were significantly lower than those in solvent control group ( P <0.01), and only the expression level of protein in high dose ODA group was significantly lower than that in 5-FU group ( P <0.01);the expression levels of P21 and caspase-9 in low, medium and high dose ODA groups and 5-FU group were significantly higher than those in solvent control group ( P <0.05, P <0.01), but the expression level of protein in high dose ODA group was significantly higher than that in 5-FU group ( P <0.01). [Conclusions] ODA can obviously inhibit the proliferation of glioma cells and induce apoptosis. The mechanism is related to up-regulation of P21, caspase-9, down-regulation of P53, PI3K, PKB/Akt, inhibition of cell division cycle and decrease of PI3K-Akt signal transduction pathway.展开更多
Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were ran...Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results: In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion: Vitamin C can protect vascular endothelial cells from mannitol-induced injury.展开更多
HailongS. acus Linnaeus was chosen as the experimental material in the experiments and changes of cell morphology, forming rate of clone and changes of division index were conducted to identify the inhibiting effects ...HailongS. acus Linnaeus was chosen as the experimental material in the experiments and changes of cell morphology, forming rate of clone and changes of division index were conducted to identify the inhibiting effects of Hailong extract on human tumor cell lines (GLT-82). Four days after medication, most of the cells changed their normal morphology of tumor cells and became round, broken and even broke into pieces. The inhibiting rates could reach 75.1% on the fourth day. The division index reduced strongly and the clone could not form or the forming rate was very low. With the extract concentration increasing and the time prolonging, the inhibiting effect increased. These results indicate that Hailong has striking anti-tumor effects and will have a perfect future in the fields of treatment and prevention of cancer.展开更多
The irregular defects and residual tumor tissue after surgery are challenges for effective breast cancer treatment.Herein,a smart hydrogel with self-adaptable size and dual responsive cargos release was fabricated to ...The irregular defects and residual tumor tissue after surgery are challenges for effective breast cancer treatment.Herein,a smart hydrogel with self-adaptable size and dual responsive cargos release was fabricated to treat breast cancer via accurate tumor elimination,on-demand adipose tissue regeneration and effective infection inhibition.The hydrogel consisted of thiol groups ended polyethylene glycol(SH-PEG-SH)and doxorubicin encapsulated mesoporous silica nanocarriers(DOX@MSNs)double crosslinked hyaluronic acid(HA)after loading of antibacterial peptides(AP)and adipose-derived stem cells(ADSCs).A pH-cleavable unsaturated amide bond was pre-introduced between MSNs and HA frame to perform the tumor-specific acidic environment dependent DOX@MSNs release,meanwhile an esterase degradable glyceryl dimethacrylate cap was grafted on MSNs,which contributed to the selective chemotherapy in tumor cells with over-expressed esterase.The bond cleavage between MSNs and HA would also cause the swelling of the hydrogel,which not only provide sufficient space for the growth of ADSCs,but allows the hydrogel to fully fill the irregular defects generated by surgery and residual tumor atrophy,resulting in the on-demand regeneration of adipose tissue.Moreover,the sustained release of AP could be simultaneously triggered along with the size change of hydrogel,which further avoided bacterial infection to promote tissue regeneration.展开更多
The protection of rusted carbon steel in acid corrosion environments is of great significance for equipment to keep safe operation.In this work,we presented a method to protect the rusted steel by ru st conversion tre...The protection of rusted carbon steel in acid corrosion environments is of great significance for equipment to keep safe operation.In this work,we presented a method to protect the rusted steel by ru st conversion treatment and epoxy coating.Tannic acid was selected as rust conversion agent.Tannic acid,D-limonene and nano-ZrO_(2)were used to improve the corrosion resistance of epoxy coatings.The Raman spectra,Xray diffraction and 3 D confocal images were used to characterize the rust conversion reaction.Ad hesion test showed that the loss of wet adhesion of the optimal coating was relatively low due to the addition of tannic acid,limonene and nano-ZrO(2).The corrosion resistance of five different coatings was investigated by scanning electron microscopy(SEM)and electrochemical analysis.Results show that after 264 h acid immersion,the low frequency resistance of the optimal coating consisting of rust conversion treatment and additives is 10^(7)Ωcm^(2),three orders magnitude higher than that of the pristine coating.Moreover,SEM indicates that the optimal coating possesses a smooth surface and an unbroken interface between substrate and coating.Accordingly,the corrosion-resistant mechanism of the hybrid coating is proposed.展开更多
The inhibition effect of an excellent environmental-friendly corrosion inhibitor--berberine on hot-dip coated steels in the diluted HCI has been investigated by using quantum chemistry analysis, mass-loss tests, elec-...The inhibition effect of an excellent environmental-friendly corrosion inhibitor--berberine on hot-dip coated steels in the diluted HCI has been investigated by using quantum chemistry analysis, mass-loss tests, elec- trochemical measurements and scanning electron microscopy （SEM） observation. Calculation results show that berberine has a nearly planar structure with a number of active centers. The value of Mulliken charge, and the distribution of the highest occupied molecular orbital （HOMO） and the lower unoccupied molecular orbital （LUMO） imply that berberine has a good ability of electron exchange with metal surface. The test results indicate that inhibition efficiency （IE%） increases with the inhibitor concentration and the highest IE can reach 99%. Adsorption of berberine on the coating surface follows Langmuir adsorption isotherm with a single molecular layer by chemisorption.展开更多
MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lip...MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lipopeptide antibiotic surfactin. The analysisby LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chainlengths of 13,14 and 15 carbon atoms. The bioactivity detection of surfactin indicatedthat the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctoniasolani and Pyricularia oryzae.展开更多
Whether rapid real estate investment growth inhibits China's innovation is a critical question for China's economic sustainability. Based on China's provincial panel data and using land supply as an instru...Whether rapid real estate investment growth inhibits China's innovation is a critical question for China's economic sustainability. Based on China's provincial panel data and using land supply as an instrumental variable for real estate investment growth, we arrived at the following findings after effectively controlling endogenous factors that could lead to estimation errors. First of all, the faster real estate investment grows in a province, the lower the growth rates of innovation and R&D spending and invention patent authorizations will be in the province, an indication that real estate directly inhibits innovation in China. Such inhibition is particularly pronounced in the industrial sectors. Secondly, in the context of rampant real estate investment, the biased loan maturity structure of China's financial system in favor of the real estate industry further inhibits innovation. This paper has verified the existence of the direct effect of real estate investment growth on regional innovation and its indirect effect on regional innovation through the biased long maturity structure of the financial system. The empirical findings of this paper provide practical policy implications for China to strike a balance between real estate development and an innovation-driven development strategy.展开更多
基金Supported by the Science and Technology Innovation Platform Construction Project of Institution of Higher Education in Hunan Province(XCJZ2009-070)the Earmarked Fund for Mordern Agro-industry Technology Research System(CARS-19-E12)~~
文摘[Objective] The aim was to explore the factors affecting the herbicidal activity of pyrazosulfuron-ethyl against Sagittaria pygmaea in paddy soil.[Method] The bioassay method was carried out under greenhouse conditions.[Result] The reduction of more than 80% in fresh weight was observed in S.pygmaea treated with 45 g/hm2 of pyrazosulfuron-ethyl for 7 d or more.Application of 60 g/hm2 at the sprouting stage of S.pygmaea suppressed completely the growth in fresh weight irrespective of tuber size.However,at 6 g/hm2 growth of the weed sustained less reduction with increasing tuber size.Differences in flooding water depth and temperature had no appreciable influence on the herbicidal activity.Growth inhibition of S.pygmaea by pre-emergence or 1.5-leaf stage applications under non-leaching conditions was significantly higher than that under water leaching conditions.Under non-leaching condition,there was no remarkable difference in this inhibition between preemergence and 1.5-leaf stage applications,while under water leaching conditions the growth reduction with the latter application was significantly larger.Growth inhibition of S.pygmaea planted at a depth of 2 cm was significantly higher than that planted deeper （5 cm） with the pre-emergence applications.[Conclusion] This study had provide basis for the further study on the inhibition effect of pyrazosulfuron-ethyl.
基金Supported by State Apple Industry Technology System ProjectDepartment of Education Research Projects in Hebei Province(2006432)~~
文摘[Objective] The study aimed to study the antifungal activity of biocontrol strains against Alternaria mali,colonization ability in apple leaves and the impact of some chemical fungicides on biocontrol strains were also studied.[Method] Healthy leaves of Matsumotokin were collected to conduct vitro leaf test,colonization test and influence test of different fungicides on biocontrol strains.[Result] Vitro leaf test showed that B.subtilis BS-315 had strong inhibition effect on A.mali,when biocontrol strain was daubed on leaves,and pathogen was inoculated after 1 d,the inhibition effect of BS-315 against A.mali was over 90% after 3 d.BS-315 could colonize in leaves of Matsumotokin,colonization volume reached the maximum after inoculation for 3 d.Azoxystrobin and polyoxin had certain promoting effect on the growth of BS-315,while carbendazim and propiconazole completely inhibited the growth of BS-315.[Conclusion] This study would provide theoretical basis for development and rational utilization of biocontrol preparations.
文摘The inhibition effect of rare earths on the production of ·O - 2 produced by radiolysis of aqueous sodium formate saturated with O 2 with high energy pulse electron beam was studied. The result indicates that rare earth nitrate obviously inhibits the production of ·O - 2 . The inhibition rate is between 28 6% and 92%. The inhibition effect increases with the increase of rare earth nitrates concentration. The distinguish dose effect relationship was observed.
文摘The phenyl and methyl phenyl free radicals,formed in the oxidation-hydrolysis process of N’-acyl-N-phenylhydrazine, have been studied by ESR spectrometry.It was found that the free radical is the chief cause which produces the feed-back inhibition effect in the hydrazine-promoted infectious development of silver halide.
文摘In controlling eutrophication phenomenon, there were conventional methods which lead to negative effects to aquatic environment. This study was aimed to investigate the usage of synthesized thiourea compounds to inhibit the growth of Oscillatoria sp. in Kenyir lake, Terengganu, Malaysia. The inhibition effects of four eco-friendly alkoxyl thiourea derivatives compounds named as N-（（4-（decyloxy）phenyl）carbamothioyl）-4-methyl benzamide, N-（（4-（decyloxy）phenyl）carbamothioyl）-4-nitro benzamide, 4-chloro-N-（（4-（decyloxy）phenyl）carbamothioyl） benzamide and N-（（4-（decyloxy）phenyl）carbamothioyl） benzamide were examined onto the growth culture of Oscillatoria sp. These compounds were tested in 30 mL of Oscillatoria sp. cultures with different concentration of 16 μg·mL-1, 18 μg·mL-1, 20 μg·mL-1, 24 μg·mL-1 and 28 μg·mL-1respectively. The treatment flasks were supplied with an aerator for 24 hours under continuous illumination at 25 ℃. Chlorophyll-a concentration were extracted to calculate the inhibition percentage of each treatment. Overall, all these compounds showed inhibition effects towards the growth of Oscillatoria sp., with the highest inhibition of 37% by N-（（4-（decyloxy）phenyl）carbamothioyl）-4-methyl benzamide at the concentration of 18μg·mL-1. The methyl group that attach to the synthesized compound may contribute to the effectiveness of the compound which act as an algae inhibitor. However, extensive studies still need to be conducted in order to investigate the mechanism on how this compound reacts with Oscillatoria sp..
文摘Objective To determine the effect of the phosphodiesterase-5 inhibitor sildenafil compared with placebo on exercise capacity and clinical status in HFPEF. Design Multicenter, double-blind, placebo-controlled, parallel-group, randomized clinical trial of 216 sta- ble outpatients with HF, ejection fraction ≥ 50%, elevated N-terminal brain-type natriuretic peptide or elevat- ed invasively measured filling pressures, and reduced exercise capacity. Participants were randomized from October 2008 through February 2012 at 26 centers in North America. Follow-up was through August 30, 2012.
文摘ABSTRACT The intensity of antiplatelet therapy during percutaneous coronary intervention （PCI） is an important deter- minant of PCI-related ischemic complications. Cangrelor is a potent intravenous adenosine diphosphate （ADP）- receptor antagonist that acts rapidly and has quickly reversible effects.
文摘Bensulfuron-Methyl(BSM)is a highly active sul-fonylurea herbicide that effectively controls most an-nual and perennial broadleaf or sedge weeds in paddyfield.However,the activity of BSM is affected byseveral evironmental factors.Using corn seedlings asmaterial,we found that under different temperatures
基金supported by the National Natural Science Foundation of China(No.42402319,51704266)the Anhui Provincial Natural Science Foundation(No.2308085QE151)+3 种基金the Natural Science Research Project of Anhui Educational Committee(No.2023AH051222)Young Talent Nurturing Program of Anhui Association For Science and Technology(No.RCTJ202403)the Open Foundation of the Innovation Base of Fine Mine Prospecting and Intelligent Monitoring Technology(No.2023-MPIM-01)partly supported by the Open Fund of Engineering Research Center of Rock-Soil Drilling&Excavation and Protection(No.202407).
文摘The stability of oil-dominated emulsions,including oil-based drilling fluids and crude oils,is crucial for mitigating gas hydrate risks in the petroleum and natural gas industries.Nanoparticles can stabilize oilwater systems(Pickering emulsions)by residing at the oil-water interface.However,their effects on the kinetics of hydrate formation in these systems remain unclear.To address this,we experimentally investigated how hydrophilic and hydrophobic nano-CaCO_(3) influence CH4 hydrate formation within dynamic oil-water systems.A series of hydrate formation experiments were conducted with varying water cuts and different concentrations of nano-CaCO_(3) at a particle size of 20 nm,under 3℃ and 6 MPa.The induction time,hydrate formation volume,and hydrate growth rate were measured and calculated.The results indicate that hydrophilic nano-CaCO_(3) generally inhibits hydrate formation,particularly at high water cuts,while hydrophobic nano-CaCO_(3) can significantly inhibit or even prevent hydrate formation at low water cuts.Water cut strongly influences the kinetics of hydrate formation,and nanoparticle concentration also impacts the results,likely due to changes in oil-water interface stability caused by nanoparticle distribution.This study will offer valuable insights for designing deepwater oilbased drilling fluids using nanoparticles and ensuring safe multiphase flow in deepwater oil and gas operations.
基金supported by the Research and Development Fund for the postdoctoral researchers of Heilongjiang Province (2012)
文摘In this work,a series of molybdovanadophosphoric heteropoly acid quaternary ammonium salts H_（3＋x）PMo_（12 -x）V_xO40-T were synthesized and employed as a reaction inhibitor in the selfpolymerization of methyl methacrylate（MMA）.The polymerization inhibition effect of H_（3＋x）PMoPMo_（12 -x）V_xO40-T）with different number of vanadium atoms and reaction dosages was investigated using differential scanning calorimetry（DSC）.It shows that the inhibitory effect was improved with the increasing dosages of H_（3＋x）PMoPMo_（12 -x）V_xO40-T）,and the polymerization inhibition was also affected by the number of vanadium atoms in the H_（3＋x）PMo_（12 -x）V_xO40-T .Furthermore,cyclic voltammograms（CV）was used to probe the mechanism of the inhibition reaction with H3＋xPMo12xVxO40-T.The result of CV indicates that the inhibition reaction is an oxidation–reduction reaction.H_（3＋x）PMo_（12 -x）V_xO40-T can react directly with the MMA monomer radicals,which eliminated the MMA monomers,and therefore the self-polymerization of the MMA can be effectively inhibited by H_（3＋x）PMo_（12 -x）V_xO40-T.
基金Project(2017210101002066)supported by the Crosswise Project of Liaoning province,China
文摘The synergistic inhibition effect of CeCl3(Ce)and serine(Ser)on the corrosion of carbon steel in a 3%NaCl solution was investigated by electrochemical methods and surface analysis.The results showed that both CeCl3 and Ser,when used alone,had limited inhibition effect toward carbon steel corrosion in the 3%NaCl solution.In contrast,the combination of CeCl3 with Ser produced a strong synergistic effect on the corrosion inhibition behavior of carbon steel,improving the inhibition efficiency significantly.The polarization curves showed that the mixture of CeCl3 and Ser acts as a cationic-type inhibitor.Scanning electron microscopy and Fourier transform infrared spectroscopy showed that the synergistic inhibition effect was due to complex formation between the cerium ions and amino acid molecules.
基金Supported by Gansu Natural Science Foundation(21JR7RA571)Lanzhou Science and Technology Plan Project(2019-1-48)Major Project of Gansu University of Political Science and Law(GZF2021XZD06).
文摘[Objectives] To explore the inhibitory effect of octadecadienoic acid (ODA) on proliferation and apoptosis of glioma cells and its mechanism. [Methods] Cultured human glioma cells (cell density 2×10^(6) cells/L) were divided into three groups: solvent control group (DMSO, 30 μL/L), 5-FU group (10 mg/L) and octadecadienoic acid group (0.3, 0.6, 1.2 mg/L). The toxic effects of ODA on glioma cells were detected by trypan blue and thiazolium blue (MTT). The expression of P53, PI3K, P21, PKB/Akt and caspase-9 protein in glioma cells were detected by enzyme-linked immunosorbent assay (ELISA). [Results] The cell count under optical microscope showed that the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group was significantly higher than that in solvent control group ( P <0.01), but there was no significant difference compared with 5-FU group ( P >0.05). The results of MTT showed that compared with the solvent control group, the inhibition rate of cell proliferation in low, medium and high dose ODA groups and 5-FU group significantly increased ( P <0.01);compared with 5-FU group, the inhibition rate of cell proliferation in high dose ODA group significantly increased ( P <0.01). The results of flow cytometry showed that compared with the solvent control group, the number of cells in G_(0)/G_(1) phase increased significantly ( P <0.05, P <0.01), the number of cells in G_(2)/M phase decreased significantly ( P <0.01) and the apoptosis rate increased significantly ( P <0.01) in the low, medium and high dose ODA groups and 5-FU group;compared with 5-FU group, the number of cells in G_(2)/M phase decreased significantly ( P <0.01) and the apoptosis rate increased significantly ( P <0.01) in ODA group. ELISA testing results showed that the expression levels of P53, P13K and PKB/Akt in low, medium and high dose ODA groups and 5-FU group were significantly lower than those in solvent control group ( P <0.01), and only the expression level of protein in high dose ODA group was significantly lower than that in 5-FU group ( P <0.01);the expression levels of P21 and caspase-9 in low, medium and high dose ODA groups and 5-FU group were significantly higher than those in solvent control group ( P <0.05, P <0.01), but the expression level of protein in high dose ODA group was significantly higher than that in 5-FU group ( P <0.01). [Conclusions] ODA can obviously inhibit the proliferation of glioma cells and induce apoptosis. The mechanism is related to up-regulation of P21, caspase-9, down-regulation of P53, PI3K, PKB/Akt, inhibition of cell division cycle and decrease of PI3K-Akt signal transduction pathway.
文摘Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results: In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion: Vitamin C can protect vascular endothelial cells from mannitol-induced injury.
文摘HailongS. acus Linnaeus was chosen as the experimental material in the experiments and changes of cell morphology, forming rate of clone and changes of division index were conducted to identify the inhibiting effects of Hailong extract on human tumor cell lines (GLT-82). Four days after medication, most of the cells changed their normal morphology of tumor cells and became round, broken and even broke into pieces. The inhibiting rates could reach 75.1% on the fourth day. The division index reduced strongly and the clone could not form or the forming rate was very low. With the extract concentration increasing and the time prolonging, the inhibiting effect increased. These results indicate that Hailong has striking anti-tumor effects and will have a perfect future in the fields of treatment and prevention of cancer.
基金the National High Level Talents Special Support Plan(X.C.)the“Young Talent Support Plan”of Xi'an Jiaotong University(X.C.)+2 种基金the Natural Science Foundation of Shaanxi Province(No.2022JZ-48 to X.C.)the National Natural Science Foundation of China(No.82272141 to X.C.)the Shaanxi Provincial Key Research and Development Plan Project(No.2023-JC-QN-0260 to X.Q.).
文摘The irregular defects and residual tumor tissue after surgery are challenges for effective breast cancer treatment.Herein,a smart hydrogel with self-adaptable size and dual responsive cargos release was fabricated to treat breast cancer via accurate tumor elimination,on-demand adipose tissue regeneration and effective infection inhibition.The hydrogel consisted of thiol groups ended polyethylene glycol(SH-PEG-SH)and doxorubicin encapsulated mesoporous silica nanocarriers(DOX@MSNs)double crosslinked hyaluronic acid(HA)after loading of antibacterial peptides(AP)and adipose-derived stem cells(ADSCs).A pH-cleavable unsaturated amide bond was pre-introduced between MSNs and HA frame to perform the tumor-specific acidic environment dependent DOX@MSNs release,meanwhile an esterase degradable glyceryl dimethacrylate cap was grafted on MSNs,which contributed to the selective chemotherapy in tumor cells with over-expressed esterase.The bond cleavage between MSNs and HA would also cause the swelling of the hydrogel,which not only provide sufficient space for the growth of ADSCs,but allows the hydrogel to fully fill the irregular defects generated by surgery and residual tumor atrophy,resulting in the on-demand regeneration of adipose tissue.Moreover,the sustained release of AP could be simultaneously triggered along with the size change of hydrogel,which further avoided bacterial infection to promote tissue regeneration.
基金supported financially by the Key Technology of Corrosion Control on Wind Power Equipment Academician Workstation Project(No.2013B090400023)。
文摘The protection of rusted carbon steel in acid corrosion environments is of great significance for equipment to keep safe operation.In this work,we presented a method to protect the rusted steel by ru st conversion treatment and epoxy coating.Tannic acid was selected as rust conversion agent.Tannic acid,D-limonene and nano-ZrO_(2)were used to improve the corrosion resistance of epoxy coatings.The Raman spectra,Xray diffraction and 3 D confocal images were used to characterize the rust conversion reaction.Ad hesion test showed that the loss of wet adhesion of the optimal coating was relatively low due to the addition of tannic acid,limonene and nano-ZrO(2).The corrosion resistance of five different coatings was investigated by scanning electron microscopy(SEM)and electrochemical analysis.Results show that after 264 h acid immersion,the low frequency resistance of the optimal coating consisting of rust conversion treatment and additives is 10^(7)Ωcm^(2),three orders magnitude higher than that of the pristine coating.Moreover,SEM indicates that the optimal coating possesses a smooth surface and an unbroken interface between substrate and coating.Accordingly,the corrosion-resistant mechanism of the hybrid coating is proposed.
基金supported by the Shandong Provincial Natural Science Foundation, China (No. ZR2009BQ016)the Fundamental Research Funds for the Central Universities, China (No. 12CX04055A)
文摘The inhibition effect of an excellent environmental-friendly corrosion inhibitor--berberine on hot-dip coated steels in the diluted HCI has been investigated by using quantum chemistry analysis, mass-loss tests, elec- trochemical measurements and scanning electron microscopy （SEM） observation. Calculation results show that berberine has a nearly planar structure with a number of active centers. The value of Mulliken charge, and the distribution of the highest occupied molecular orbital （HOMO） and the lower unoccupied molecular orbital （LUMO） imply that berberine has a good ability of electron exchange with metal surface. The test results indicate that inhibition efficiency （IE%） increases with the inhibitor concentration and the highest IE can reach 99%. Adsorption of berberine on the coating surface follows Langmuir adsorption isotherm with a single molecular layer by chemisorption.
基金supported by the National Nature1 Science Foundation of China(30170623)the National 863 Program of China(2001AA246013).
文摘MALDI-TOF-MS technology was used for identification of lipopeptide antibiotics producedby GEB3 strain, a derivative of Bacillus subtilis 168 which was transformed by lpaB3gene. The result showed GEB3 only produced lipopeptide antibiotic surfactin. The analysisby LC-MS demonstrated that GEB3 produced standard surfactin isoforms with side chainlengths of 13,14 and 15 carbon atoms. The bioactivity detection of surfactin indicatedthat the surfactin produced by GEB3 had inhibition effect on plant pathogens Rhizoctoniasolani and Pyricularia oryzae.
基金the sponsorship of the Ministry of Education Program“Navigating the New Normal”Scientific Research Foundation of the Renmin University of China“A Study on Limited Catch Up and Transition of China's Trade Pattern:A New Theory Based on Global Trade Scale and Interest Disequilibrium(Grant No.12XNI010)”
文摘Whether rapid real estate investment growth inhibits China's innovation is a critical question for China's economic sustainability. Based on China's provincial panel data and using land supply as an instrumental variable for real estate investment growth, we arrived at the following findings after effectively controlling endogenous factors that could lead to estimation errors. First of all, the faster real estate investment grows in a province, the lower the growth rates of innovation and R&D spending and invention patent authorizations will be in the province, an indication that real estate directly inhibits innovation in China. Such inhibition is particularly pronounced in the industrial sectors. Secondly, in the context of rampant real estate investment, the biased loan maturity structure of China's financial system in favor of the real estate industry further inhibits innovation. This paper has verified the existence of the direct effect of real estate investment growth on regional innovation and its indirect effect on regional innovation through the biased long maturity structure of the financial system. The empirical findings of this paper provide practical policy implications for China to strike a balance between real estate development and an innovation-driven development strategy.
