Objective Sepsis patients exhibit diverse immune states,making it crucial to identify subtypes with distinct inflammatory profiles through Th1/Th2 cytokine data for personalized treatment and improved prognosis.Method...Objective Sepsis patients exhibit diverse immune states,making it crucial to identify subtypes with distinct inflammatory profiles through Th1/Th2 cytokine data for personalized treatment and improved prognosis.Methods We retrieved data from sepsis patients who underwent Th1/Th2 cytokine testing in Nanfang Hospital,Southern Medical University from June 1,2020,to February 1,2022.An unsupervised K-means clustering method classified participants based on Th1/Th2 cytokine levels,with the primary outcome being the 7-day mortality rate post-ICU admission.Cox proportional hazards and Restricted Mean Survival Time(RMST)analyses were utilized to explore survival outcomes.Results A total of 321 sepsis patients were included.IL-6(HR 1.69,95%CI:1.22,2.34)and IL-10(HR 1.81,95%CI:1.37,2.40)emerged as independent predictors of 7-day mortality.Unsupervised K-means clustering revealed 3 inflammatory/immune subgroups:Cluster 1(n=166,low inflammatory response),Cluster 2(n=99,moderate inflammatory response with immune suppression),and Cluster 3(n=56,strong inflammatory and immune suppression).Compared to Cluster 1,Clusters 2 and 3 had higher 7-day mortality risks(14.4%vs 23.2%,HR=4.30,95%CI:1.51-12.26;14.4%vs 35.7%,HR=7.32,95%CI:2.57-20.79).Conclusion Septic patients in a protective immune response state(Cluster 1)exhibit better short-term prognoses,suggesting the importance of understanding inflammatory/immune states for precise treatment and improved outcomes.展开更多
Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different is...Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different ischemia/reperfusion(I/R)time points.The proliferation was reflected by CCK-8assay and cell number counting.The differentiation was reflected by cell shape and CK-18RNA expression.Results After 15dculture,shape of MSCs cultured with kidney homogenate at 72htime point was much more round and ellipsoidal,and the cells had a cobble like appearance.Cell number,CCK-8and CK-18level of MSCs cultured with kidney homogenate at 72htime point was significantly higher comparing with other time points.Conclusions 72hpost I/R was the optimal timing for proliferation and differentiation of MSCs.展开更多
文摘Objective Sepsis patients exhibit diverse immune states,making it crucial to identify subtypes with distinct inflammatory profiles through Th1/Th2 cytokine data for personalized treatment and improved prognosis.Methods We retrieved data from sepsis patients who underwent Th1/Th2 cytokine testing in Nanfang Hospital,Southern Medical University from June 1,2020,to February 1,2022.An unsupervised K-means clustering method classified participants based on Th1/Th2 cytokine levels,with the primary outcome being the 7-day mortality rate post-ICU admission.Cox proportional hazards and Restricted Mean Survival Time(RMST)analyses were utilized to explore survival outcomes.Results A total of 321 sepsis patients were included.IL-6(HR 1.69,95%CI:1.22,2.34)and IL-10(HR 1.81,95%CI:1.37,2.40)emerged as independent predictors of 7-day mortality.Unsupervised K-means clustering revealed 3 inflammatory/immune subgroups:Cluster 1(n=166,low inflammatory response),Cluster 2(n=99,moderate inflammatory response with immune suppression),and Cluster 3(n=56,strong inflammatory and immune suppression).Compared to Cluster 1,Clusters 2 and 3 had higher 7-day mortality risks(14.4%vs 23.2%,HR=4.30,95%CI:1.51-12.26;14.4%vs 35.7%,HR=7.32,95%CI:2.57-20.79).Conclusion Septic patients in a protective immune response state(Cluster 1)exhibit better short-term prognoses,suggesting the importance of understanding inflammatory/immune states for precise treatment and improved outcomes.
文摘Objective To investigate the optimal timing of acute kidney injury environment for proliferation and differentiation of mesenchymal stem cells(MSCs).Methods MSCs were co-cultured with kidney homogenate at different ischemia/reperfusion(I/R)time points.The proliferation was reflected by CCK-8assay and cell number counting.The differentiation was reflected by cell shape and CK-18RNA expression.Results After 15dculture,shape of MSCs cultured with kidney homogenate at 72htime point was much more round and ellipsoidal,and the cells had a cobble like appearance.Cell number,CCK-8and CK-18level of MSCs cultured with kidney homogenate at 72htime point was significantly higher comparing with other time points.Conclusions 72hpost I/R was the optimal timing for proliferation and differentiation of MSCs.
