Objective:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.Methods:Sixty SD rat aged 9-12 weeks were chosen and divide...Objective:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.Methods:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group,model group and simvastatintreated group randomly with 20 rats in each group.Rats in the model group and simvastatintreated group were infused with autologous fresh uncoagulated blood to the right brain tissue of the basal ganglia to build the cerebral hemorrhage model,while rats in the control group were treated with the same amount of normal saline.Then,rats in the simvastatin-treated group were given a gavage of 3 mg/kg of simvastatin once a day after modeling.Rats in the three groups were given nerve dysfunction score(NDS) and wet-dry weighting method was used to detect the brain water content(BWC) of brain tissues around the lesion of the rats.Then Nissl staining was conducted and the undamaged neurons were counted.Immunohistochemical SP method was applied to count the number of NF-d the immuno fluorκB,TLR4 and IL-1escence method wasβ positive cells in brain tissues around the lesions,an employed to determine the expression levels of NF-κB,TLR4 and IL-1me points were aβ proteins.Results:The NDS results of the simvastatin-treated group at all till significantly higher than those of the model group(P < 0.05);the BWC values of the simvastatin-treated group at all time points were all significantly lower than those of the model group at the same periods(P < 0.05);the number of the undamaged neurons around the lesions of the simvastatin-treated group at all time points were all significantly higher than those of the model group(P < 0.05);seven days after treatment,the number of the NF-κB,TLR4 and IL-1β positive cells in brain tissues around the lesions of the simvastatin-treated group were all significantly lower than those of the model group(P < 0.05),and its expression levels of NF-ower than those of the model group(κB,TLR4 and IL-1P < 0.05).Conclusioβ protein were also significantly lns:Simvastatin can inhibit the expressions of NF-κB,TLR4 and IL-1β proteins in rats with cerebral hemorrhage,and protect neurons and reduce secondary inflammatory damages by down-regulating the above protein-mediated inflammatory responses.展开更多
Objective: To evaluate the efficacy of Sheng-mai Power (SMP) in treating acute viral myocarditis. Methods: 102 patients with acute viral myocarditis were randomized to SMS group (n = 52) and placebo control group (n =...Objective: To evaluate the efficacy of Sheng-mai Power (SMP) in treating acute viral myocarditis. Methods: 102 patients with acute viral myocarditis were randomized to SMS group (n = 52) and placebo control group (n = 50 ). Semiquantitative integral methods were taken to observe changes of clinical symptoms such as dyspnea, palpitation and chest pain after 4 weeks of treatment, simultaneously EKG, 24h Holter, concentration of serum cardiac troponin-I, cardiac troponin T and neutralizing antibody test to the Coxsackie B virus were determined. Results: Dyspnea improved much more obvious in SMP group than in the placebo展开更多
Ultraviolet B(UVB)radiation causes skin photoaging and induces skin inflammation.Forsythiaside A(FTA)has a rich pharmacological effect and shows good anti-inflammatory activity in many aspects.However,the role or unde...Ultraviolet B(UVB)radiation causes skin photoaging and induces skin inflammation.Forsythiaside A(FTA)has a rich pharmacological effect and shows good anti-inflammatory activity in many aspects.However,the role or underlying mechanism of FTA in UVB-induced inflammatory skin lesions has not been reported.This study in-vestigates the mechanisms underlying FTA’s anti-inflammatory effects using in vitro and in vivo models as well as RNA-seq.In vitro,a UVB-HaCaT cell damage model revealed that FTA reduces inflammatory factors and matrix metalloproteinases(MMPs),restores skin barrier-related factor levels,and effectively repairs inflammation-induced cellular damage.Transcriptome analysis identified the IL-17 signaling pathway as a key anti-inflammatory target.FTA inhibited gene and protein expression in this pathway,leading to reduced inflam-mation and cell damage repair.In vivo,FTA alleviated skin damage in mice caused by DNCB,reducing erythema,scales,and skin thickness while maintaining skin structure.Molecular analyses(Western blot and qRT-PCR)confirmed decreased expression of inflammatory proteins and genes.These results suggest that FTA inhibits IL-17 signaling pathway and repairs skin inflammatory damage.It is concluded that FTA has great potential to resist skin inflammatory damage and photoaging.展开更多
BACKGROUND Patients with severe aplastic anemia(SAA)frequently present with inflammatory episodes,and during flared inflammatory episodes,hematopoietic function is further exacerbated.The gastrointestinal tract is the...BACKGROUND Patients with severe aplastic anemia(SAA)frequently present with inflammatory episodes,and during flared inflammatory episodes,hematopoietic function is further exacerbated.The gastrointestinal tract is the most common site for infectious and inflammatory diseases,and its structural and functional features confer on it the most potent capacity to affect hematopoietic and immune functions.Computed tomography(CT)is a readily accessible approach to provide highly useful information in detecting morphological changes and guiding further work-ups.AIM To explore CT imaging presentations of gut inflammatory damage in adult SAA patients during inflammatory episodes.METHODS We retrospectively evaluated the abdominal CT imaging presentations of 17hospitalized adult patients with SAA in search of the inflammatory niche when they presented with systemic inflammatory stress and exacerbated hematopoietic function.In this descriptive manuscript,the characteristic images that suggested the presence of gastrointestinal inflammatory damage and related imaging presentations of individual patients were enumerated,analyzed and described.RESULTS All eligible patients with SAA had CT imaging abnormalities that suggested the presence of an impaired intestinal barrier and increased epithelial permeability.The inflammatory damages were concurrently present in the small intestine,the ileocecal region and the large intestines.Some readily identified imaging signs,such as bowel wall thickening with mural stratification(“water holo sign”,“fat holo sign”,intramural gas and subserosal pneumatosis)and mesenteric fat proliferation(fat stranding and“creeping fat sign”),fibrotic bowel wall thickening,“balloon sign”,rugged colonic configuration,heterogeneity in the bowel wall texture,and adhered and clustered small bowel loop(including various patterns of“abdominal cocoon”),occurred at a high incidence,which suggested that the damaged gastrointestinal tract is a common inflammatory niche responsible for the systemic inflammatory stresses and the exacerbated hematopoietic failure in patients with SAA.Particularly,the“fat holo sign”was present in 7 patients,a rugged colonic configuration was present in 10 patients,the adhesive bowel loop was present in 15 patients,and extraintestinal manifestations suggestive of tuberculosis infections were present in 5 patients.According to the imaging features,a suggestive diagnosis of Crohn’s disease was made in 5patients,ulcerative colitis in 1 patient,chronic periappendiceal abscess in 1 patient,and tuberculosis infection in 5 patients.Other patients were diagnosed with chronic enteroclolitis with acutely aggravated inflammatory damage.CONCLUSION Patients with SAA had CT imaging patterns that suggested the presence of active chronic inflammatory conditions and aggravated inflammatory damage during flared inflammatory episodes.展开更多
Alcoholic liver injury is a prevalent health issue worldwide,characterized by oxidative stress,inflammation,and gut microbiota imbalance.Traditional treatments often focus on reducing alcohol intake and managing sympt...Alcoholic liver injury is a prevalent health issue worldwide,characterized by oxidative stress,inflammation,and gut microbiota imbalance.Traditional treatments often focus on reducing alcohol intake and managing symptoms,but novel therapeutic approaches targeting gut microbiota and oxidative stress are gaining attention.Highland barley functional tea(HBFT)extract was known for its antioxidant and anti-inflammatory properties,but its effects on alcoholic liver injury and gut microbiota remained unclear.This study aimed to evaluate the protective effects of HBFT on alcoholic liver injury and elucidated its underlying mechanisms of action.Male C57BL/6 J mice were divided into six groups(n=12):control,model(0.2 mL/20 g per day),positive drug(0.2 mL/20 g per day),low-dose HBFT(1.4 g/kg per day),medium-dose HBFT(2.8 g/kg per day),and high-dose HBFT(5.6 g/kg per day).The findings indicated that HBFT intervention significantly inhibited the increase in ALT and AST serum levels.HBFT also enhanced the activity of enzymes and expression of proteins associated with oxidative stress,inflammatory damage,and hepatic lipid metabolism.Furthermore,HBFT promoted the proliferation of beneficial gut bacteria in mice,such as Akkermansia and Lactobacillus.Moreover,the HBFT intervention helped restore the levels of short-chain fatty acids(SCFAs)in mice caused by alcohol exposure.Taken together,these results suggested that HBFT extract may act as a novel gut microbiota modulator,alleviating alcoholic liver injury by restoring gut microbial balance and ameliorating hepatic oxidative stress and inflammation.展开更多
基金supported by Hebei Social Science Fund Project in 2016(Grant No.HB16LJ006)the Dr. Start-up Fund of North China University of Science and Technology(2015)
文摘Objective:To investigate the preventive and therapeutic effect and mechanism of simvastatin on secondary inflammatory damage of rats with cerebral hemorrhage.Methods:Sixty SD rat aged 9-12 weeks were chosen and divided into the control group,model group and simvastatintreated group randomly with 20 rats in each group.Rats in the model group and simvastatintreated group were infused with autologous fresh uncoagulated blood to the right brain tissue of the basal ganglia to build the cerebral hemorrhage model,while rats in the control group were treated with the same amount of normal saline.Then,rats in the simvastatin-treated group were given a gavage of 3 mg/kg of simvastatin once a day after modeling.Rats in the three groups were given nerve dysfunction score(NDS) and wet-dry weighting method was used to detect the brain water content(BWC) of brain tissues around the lesion of the rats.Then Nissl staining was conducted and the undamaged neurons were counted.Immunohistochemical SP method was applied to count the number of NF-d the immuno fluorκB,TLR4 and IL-1escence method wasβ positive cells in brain tissues around the lesions,an employed to determine the expression levels of NF-κB,TLR4 and IL-1me points were aβ proteins.Results:The NDS results of the simvastatin-treated group at all till significantly higher than those of the model group(P < 0.05);the BWC values of the simvastatin-treated group at all time points were all significantly lower than those of the model group at the same periods(P < 0.05);the number of the undamaged neurons around the lesions of the simvastatin-treated group at all time points were all significantly higher than those of the model group(P < 0.05);seven days after treatment,the number of the NF-κB,TLR4 and IL-1β positive cells in brain tissues around the lesions of the simvastatin-treated group were all significantly lower than those of the model group(P < 0.05),and its expression levels of NF-ower than those of the model group(κB,TLR4 and IL-1P < 0.05).Conclusioβ protein were also significantly lns:Simvastatin can inhibit the expressions of NF-κB,TLR4 and IL-1β proteins in rats with cerebral hemorrhage,and protect neurons and reduce secondary inflammatory damages by down-regulating the above protein-mediated inflammatory responses.
文摘Objective: To evaluate the efficacy of Sheng-mai Power (SMP) in treating acute viral myocarditis. Methods: 102 patients with acute viral myocarditis were randomized to SMS group (n = 52) and placebo control group (n = 50 ). Semiquantitative integral methods were taken to observe changes of clinical symptoms such as dyspnea, palpitation and chest pain after 4 weeks of treatment, simultaneously EKG, 24h Holter, concentration of serum cardiac troponin-I, cardiac troponin T and neutralizing antibody test to the Coxsackie B virus were determined. Results: Dyspnea improved much more obvious in SMP group than in the placebo
基金Funded by Beijing Technology and Business University 2024 Grad-uate Research Ability Improvement Plan(19008024042).
文摘Ultraviolet B(UVB)radiation causes skin photoaging and induces skin inflammation.Forsythiaside A(FTA)has a rich pharmacological effect and shows good anti-inflammatory activity in many aspects.However,the role or underlying mechanism of FTA in UVB-induced inflammatory skin lesions has not been reported.This study in-vestigates the mechanisms underlying FTA’s anti-inflammatory effects using in vitro and in vivo models as well as RNA-seq.In vitro,a UVB-HaCaT cell damage model revealed that FTA reduces inflammatory factors and matrix metalloproteinases(MMPs),restores skin barrier-related factor levels,and effectively repairs inflammation-induced cellular damage.Transcriptome analysis identified the IL-17 signaling pathway as a key anti-inflammatory target.FTA inhibited gene and protein expression in this pathway,leading to reduced inflam-mation and cell damage repair.In vivo,FTA alleviated skin damage in mice caused by DNCB,reducing erythema,scales,and skin thickness while maintaining skin structure.Molecular analyses(Western blot and qRT-PCR)confirmed decreased expression of inflammatory proteins and genes.These results suggest that FTA inhibits IL-17 signaling pathway and repairs skin inflammatory damage.It is concluded that FTA has great potential to resist skin inflammatory damage and photoaging.
基金Supported by the Specialized Scientific Research Fund Projects of the Medical Group of Qingdao University,No.YLJT20201002。
文摘BACKGROUND Patients with severe aplastic anemia(SAA)frequently present with inflammatory episodes,and during flared inflammatory episodes,hematopoietic function is further exacerbated.The gastrointestinal tract is the most common site for infectious and inflammatory diseases,and its structural and functional features confer on it the most potent capacity to affect hematopoietic and immune functions.Computed tomography(CT)is a readily accessible approach to provide highly useful information in detecting morphological changes and guiding further work-ups.AIM To explore CT imaging presentations of gut inflammatory damage in adult SAA patients during inflammatory episodes.METHODS We retrospectively evaluated the abdominal CT imaging presentations of 17hospitalized adult patients with SAA in search of the inflammatory niche when they presented with systemic inflammatory stress and exacerbated hematopoietic function.In this descriptive manuscript,the characteristic images that suggested the presence of gastrointestinal inflammatory damage and related imaging presentations of individual patients were enumerated,analyzed and described.RESULTS All eligible patients with SAA had CT imaging abnormalities that suggested the presence of an impaired intestinal barrier and increased epithelial permeability.The inflammatory damages were concurrently present in the small intestine,the ileocecal region and the large intestines.Some readily identified imaging signs,such as bowel wall thickening with mural stratification(“water holo sign”,“fat holo sign”,intramural gas and subserosal pneumatosis)and mesenteric fat proliferation(fat stranding and“creeping fat sign”),fibrotic bowel wall thickening,“balloon sign”,rugged colonic configuration,heterogeneity in the bowel wall texture,and adhered and clustered small bowel loop(including various patterns of“abdominal cocoon”),occurred at a high incidence,which suggested that the damaged gastrointestinal tract is a common inflammatory niche responsible for the systemic inflammatory stresses and the exacerbated hematopoietic failure in patients with SAA.Particularly,the“fat holo sign”was present in 7 patients,a rugged colonic configuration was present in 10 patients,the adhesive bowel loop was present in 15 patients,and extraintestinal manifestations suggestive of tuberculosis infections were present in 5 patients.According to the imaging features,a suggestive diagnosis of Crohn’s disease was made in 5patients,ulcerative colitis in 1 patient,chronic periappendiceal abscess in 1 patient,and tuberculosis infection in 5 patients.Other patients were diagnosed with chronic enteroclolitis with acutely aggravated inflammatory damage.CONCLUSION Patients with SAA had CT imaging patterns that suggested the presence of active chronic inflammatory conditions and aggravated inflammatory damage during flared inflammatory episodes.
基金supported by Central Leading Local Science and Technology Development Fund Project in Qinghai Province(2024ZY027)Xining Science and Technology Planning Program(2024-Y-13).
文摘Alcoholic liver injury is a prevalent health issue worldwide,characterized by oxidative stress,inflammation,and gut microbiota imbalance.Traditional treatments often focus on reducing alcohol intake and managing symptoms,but novel therapeutic approaches targeting gut microbiota and oxidative stress are gaining attention.Highland barley functional tea(HBFT)extract was known for its antioxidant and anti-inflammatory properties,but its effects on alcoholic liver injury and gut microbiota remained unclear.This study aimed to evaluate the protective effects of HBFT on alcoholic liver injury and elucidated its underlying mechanisms of action.Male C57BL/6 J mice were divided into six groups(n=12):control,model(0.2 mL/20 g per day),positive drug(0.2 mL/20 g per day),low-dose HBFT(1.4 g/kg per day),medium-dose HBFT(2.8 g/kg per day),and high-dose HBFT(5.6 g/kg per day).The findings indicated that HBFT intervention significantly inhibited the increase in ALT and AST serum levels.HBFT also enhanced the activity of enzymes and expression of proteins associated with oxidative stress,inflammatory damage,and hepatic lipid metabolism.Furthermore,HBFT promoted the proliferation of beneficial gut bacteria in mice,such as Akkermansia and Lactobacillus.Moreover,the HBFT intervention helped restore the levels of short-chain fatty acids(SCFAs)in mice caused by alcohol exposure.Taken together,these results suggested that HBFT extract may act as a novel gut microbiota modulator,alleviating alcoholic liver injury by restoring gut microbial balance and ameliorating hepatic oxidative stress and inflammation.
