Introduction/Purpose: The “transverse abdominal plane block” or TAP block was described by Rafi in 2001. It describes an approach to the neurofascial plane of the transverse abdominal muscle via the Jean-Louis Petit...Introduction/Purpose: The “transverse abdominal plane block” or TAP block was described by Rafi in 2001. It describes an approach to the neurofascial plane of the transverse abdominal muscle via the Jean-Louis Petit triangle and provides analgesia of the entire homolateral hemi abdomen. The aim of our study was to compare post-operative analgesia and post-operative morphine requirements between transverse abdominal plane block (TAP) and peri-orificial infiltration during laparoscopic abdominal surgery. Material and method: Prospective, randomized study conducted over a 2-year period. The study was conducted in the operating theatre of the Saint Louis Regional Hospital in Senegal. All adult patients undergoing laparoscopic abdominal surgery were included. Clinical aspects, pain scales and morphine consumption were analyzed. Results: A total of 60 patients were enrolled: 30 patients in the TAP group and 30 patients in the infiltration group. The average age was 32.9 years. The indications for laparoscopy were acute appendicitis in 50% of cases, gallbladder stones in 16% and inguinal hernia in 8%. For the TAP group, the mean numerical pain scale was 3.9 at 6 hours post-operatively and 2.1 at 24 hours post-operatively. For the infiltration group, the mean numerical pain scale was 4.3 at 6 hours post-op and 3 at 24 hours post-op. Morphine consumption at 6 hours post-op was on average 0.4 mg/patient for the TAP group and 0.9 mg/patient for the infiltration group. Discussion/conclusion: Analgesia provided by ultrasound-guided TAP block for laparoscopic abdominal surgery appears to be identical to periorificial infiltration. However, the simplicity and reproducibility of ultrasound-guided TAP block gives it a definite advantage.展开更多
Background:Heat shock protein B8(HSPB8)is implicated in autophagy,and its aberrant expression has been linked to both the ini-tiation and progression of tumors.However,the role and function of HSPB8 in colorectal canc...Background:Heat shock protein B8(HSPB8)is implicated in autophagy,and its aberrant expression has been linked to both the ini-tiation and progression of tumors.However,the role and function of HSPB8 in colorectal cancer(CRC)and across multiple cancer types remain unclear.This study aimed to map the transcriptome of autophagy-related genes in CRC and to conduct a pan-cancer analysis of HSPB8 as both a prognostic and immunological biomarker.Methods:We performed bioinformatics analyses on GSE113513 and GSE74602 to identify differentially expressed genes(DEGs)in CRC.These DEGs were then compared with autophagy-related genes to identify critical overlapping genes.The Kaplan-Meier plotter was used to verify the ex-pression of autophagy-linked DEGs and evaluate its prognostic value.The protein expression of Hub gene in CRC was analyzed using the Human Protein Atlas database.The cBioPortal was used to analyze the type and frequency of Hub gene mutations.The TIMER(Tumor Immune Estimation Resource)database was used to study the correlation between HSPB8 and immune infiltration in CRC.Results:In total,825 DEGs were identified,including 8 autophagy-linked DEGs:ATIC,MYC,HSPB8,TNFSF10,BCL2,TP53INP2,ITPR1,and NKX2-3.Survival analysis showed that increased HSPB8 expression significantly correlates with poor prognosis in patients with CRC(p<0.05).HSPB8 was also found to be differentially expressed in various cancer types,correlating with both prognosis and immune infiltration.Further,changes in HSPB8 methylation and phosphorylation status were observed across several cancers,suggesting potential regulatory mechanisms.Therefore,HSPB8 may serve as a crucial prognostic and immunological biomarker in CRC and other cancers.Conclusions:This study provides new insights into the role of autophagy-related genes in cancer progression and highlights HSPB8 as a potential target for cancer diagnostics and therapy.展开更多
To effectively regulate the grain boundary infiltration in CoCrFeMnNi high-entropy alloy(Cantor alloys,HEA)caused by the violent atomic interdiffusion,the higher configuration entropy on Cantor alloys surface was desi...To effectively regulate the grain boundary infiltration in CoCrFeMnNi high-entropy alloy(Cantor alloys,HEA)caused by the violent atomic interdiffusion,the higher configuration entropy on Cantor alloys surface was designed and realized via eutectic high-entropy(EHEA)transformation.Meanwhile,to effectively alleviate the residual stress caused by the notable difference in the thermal expansion coefficient(CTE)between Cantor alloys and Zr-3 alloys,a cladding layer was applied to the HEA surface using laser cladding technology of Nb,followed by brazing to Zr-3 alloys with Zr63.2Cu filler.The cladding layer’s microstructure comprised Nbss and FCC+(Co,Ni)_(2) Nb eutectic structure,resulting from an in-situ reaction between Cantor alloys and Nb.The Nbss and FCC demonstrated good plasticity,and the(Co,Ni)_(2) Nb Laves phase provided increased strength,endowing both good plastic deformation ability and strength of the cladding layer.Notably,the existence of EHEA in the laser cladding layer made the Cantor alloy entropy from 1.61 R to 1.77 R,greatly enhancing its thermal stability and suppressing the grave grain boundary infiltration.Joints produced via laser cladding with Nb-assisted brazing exhibited a complex microstructure(HEA/Nbss+FCC+(Co,Ni)_(2)Nb/(Zr,Nb)(Cr,Mn)_(2)+(Zr,Nb)ss/(Zr,Nb)_(2)(Cu,Ni,Co,Fe)+(Zr,Nb)(Cr,Mn)_(2)+(Zr,Nb)ss/Zr-3) and a significantly improved shear strength of 242.8 MPa at 1010℃ for 10 min,42.4%higher than that of directly brazed joints.This improvement was attributed to reduced grain boundary infiltration,alleviated residual stress due to CTE disparity,and eliminated micro-cracks in the brazing seam.This study presents an effective solution for reducing residual stresses and achieving reliable bonding between Cantor alloys and Zr-3 alloys,with potential applications in brazing CoCrFeNi-based HEA and Zr-3 due to the beneficial eutectic reaction between CoCrFeNi and Nb.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in ...BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.展开更多
Background:NDC80 is pivotal in cell division,particularly in regulating the G2/M transition and mitotic progression.Recent studies have demonstrated that NDC80 is significantly overexpressed in multiple solid tumors.F...Background:NDC80 is pivotal in cell division,particularly in regulating the G2/M transition and mitotic progression.Recent studies have demonstrated that NDC80 is significantly overexpressed in multiple solid tumors.Further analysis has suggested that its high expression is significantly associated with an elevated pathological grade,increased metastatic risk,and shortened overall survival in patients with cancer.However,its precise role in pan-cancer development,progression,and prognosis remains unclear.Methods:We conducted a multi-omics analysis of NDC80 using genomic,transcriptomic,and proteomic data from 33 cancer types in The Cancer Genome Atlas,Clinical Proteomic Tumor Analysis Consortium,Genotype-Tissue Expression,and Human Protein Atlas.Results:The results demonstrated frequent NDC80 mutations across multiple malignancies and significantly elevated expression in tumor tissues compared with that in their normal counterparts,correlating with worse overall and disease-free survival.Moreover,NDC80 expression was strongly associated with oncogenic pathways,key protein regulators,cellular components,myeloid-derived suppressor cell infiltration,ESTIMATE scores,and cancer-related signaling networks.Conclusions:These findings underscore the potential of NDC80 as a prognostic biomarker and therapeutic target for cancer treatment.展开更多
To the Editor:Pancreatic cancer is a malignancy characterized by a poor prog-nosis,with a 5-year survival rate of<10%[1].Furthermore,only a minority of patients(<20%)qualify for curative-intent resec-tion,and ev...To the Editor:Pancreatic cancer is a malignancy characterized by a poor prog-nosis,with a 5-year survival rate of<10%[1].Furthermore,only a minority of patients(<20%)qualify for curative-intent resec-tion,and even among those who undergo this procedure,the risk of recurrence within three years remains alarmingly high,reach-ing up to 70%[2].Due to the lack of specific clinical manifes-tations of pancreatic cancer,most cases have metastasized or in-vaded the major vessels around the pancreas at the time of initial diagnosis,resulting in a low surgical resection rate.Even patients who undergo surgical resection often face a poor prognosis[3].In recent years,neoadjuvant chemotherapy using agents such as gemcitabine,5-fluorouracil,albumin-bound paclitaxel,modified fluorouracil/leucovorin plus irinotecan,and oxaliplatin(mFOLFIRI-NOX),targeted therapies addressing molecular subtypes of pan-creatic cancer,and immunotherapies targeting PD-1 and PD-L1 have shown efficacy in improving the overall prognosis of patients with pancreatic cancer,although the impact remains modest[4,5].Therefore,novel therapeutic strategies and prognostic evaluation systems are urgently needed to enhance the survival of patients with pancreatic cancer.展开更多
Empirical formulas are indispensable tools in water engineering and hydraulic structure design.Derived from meticulous field observations,experiments,and diverse datasets,these formulas help to estimate water leakage ...Empirical formulas are indispensable tools in water engineering and hydraulic structure design.Derived from meticulous field observations,experiments,and diverse datasets,these formulas help to estimate water leakage in structures such as dams,tunnels,canals,and pipelines.By utilizing a few easily measurable parameters,engineers can employ these formulas to generate preliminary leakage rate estimates before proceeding with more detailed analyses.In this study,a physical model was developed,and a series of experiments were conducted,considering variables such as inflow rate,materials constituting the unsaturated medium,and variations in infiltration trench depth and width.As a result,a novel artificial recharge method was introduced,and an empirical equation,Q_(out)=0.0066×D_(50)^(0.64)×L×P^(0.36),was proposed to estimate the infiltration capacity of the trench.This equation incorporates factors such as the wetted perimeter,mean soil particle diameter,trench length,and a coefficient.A comparative analysis between the observed data from nine Iranian earthen canals and the values calculated using the proposed equation revealed an average relative error of 15%between the two datasets.In addition,the Pearson correlation coefficient was determined to be 0.981 and the Root Mean Square Error(RMSE)was 0.381,demonstrating the strong predictive performance of the equation.The parameters considered in the proposed equation allow for its application across diverse regions.Given its accurate performance,this equation provides a reliable initial estimate of the leakage rate,thereby helping to reduce costs and save time.展开更多
BACKGROUND Acute liver failure(ALF)due to diffuse hepatic infiltration by metastatic mela-noma is extremely rare and often misdiagnosed.In the absence of prior malig-nancy,this presentation can mimic other hepatic eme...BACKGROUND Acute liver failure(ALF)due to diffuse hepatic infiltration by metastatic mela-noma is extremely rare and often misdiagnosed.In the absence of prior malig-nancy,this presentation can mimic other hepatic emergencies such as Budd-Chiari syndrome.Early identification is crucial,especially in transplant candidates,to prevent inappropriate management.CASE SUMMARY A 61-year-old male presented with jaundice,abdominal distension,and enceph-alopathy.Liver imaging suggested acute Budd-Chiari syndrome,and liver trans-plantation was considered.However,biopsy revealed extensive hepatic infilt-ration by human melanoma black-positive melanoma cells.There was no known can-cer history,although retrospective symptoms suggested uveal localization as a possi-ble primary site.The patient rapidly deteriorated and died.A review of 12 simi-lar cases revealed shared diagnostic challenges,frequent misdiagnoses,and poor outcomes.CONCLUSION Infiltrative melanoma should be considered in unexplained ALF,even without previously known malignancy.展开更多
Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential bio...Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential biomarkers delineating the progression and metastatic cascade of NSCLC assumes paramount importance in fostering advancements toward enhanced patient outcomes and prognostic stratification.Methods:The expression level of the actin-related protein 2/3 complex;subunit 1A(ARPC1A)in NSCLC was evaluated using The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis(GEPIA)databases;along with the LinkedOmics database for co-expression genes.Further verification of ARPC1A expression in normal lung cells and NSCLC cells;as well as in normal tissues and lung cancer tissues;was performed using quantitative real-time reverse transcription PCR(RTqPCR)and Western blotting.The function of ARPC1A was explored through Gene Set Enrichment Analysis(GSEA)and immune infiltration analysis;followed by functional experiments for validation.Results:ARPC1A is upregulated in NSCLC and is associated with unfavorable clinical prognoses.Additionally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis highlights a potential link between the ARPC1A gene and the cell cycle and p53 signaling pathways.ARPC1A also promotes cell proliferation and resistance to chemotherapeutic drugs,thereby enhancing the oncogenic potential of NSCLC.Relevant cell-based experiments confirm that targeted inhibition of ARPC1A effectively suppresses cellular migratory and invasive capabilities.The immune infiltration analysis showed a close association between ARPC1A expression and various immune components,suggesting ARPC1A may interact with the tumor microenvironment.Mechanistically,ARPC1A promotes cell migration by stimulating the epithelialto-mesenchymal transition(EMT).Conclusion:The study results revealed the potential of ARPC1A as a valuable prognostic biomarker for NSCLC.Additionally,the associated mechanisms provide insights that may pave the way for therapeutic interventions for NSCLC patients.展开更多
BACKGROUND Shwachman-Diamond syndrome(SDS)is a rare genetic disorder that affects multiple organs,primarily the liver.Most patients are diagnosed during infancy or early childhood.As they grow older,the majority of af...BACKGROUND Shwachman-Diamond syndrome(SDS)is a rare genetic disorder that affects multiple organs,primarily the liver.Most patients are diagnosed during infancy or early childhood.As they grow older,the majority of affected children may experience spontaneous remission,and cases of cirrhosis in adults are rarely reported.CASE SUMMARY A 36-year-old male patient presented with massive ascites.Laboratory tests revealed pancytopenia and a serum-ascites albumin gradient greater than 1.1 g/dL.An abdominal computed tomography scan demonstrated cirrhosis,splenomegaly,pancreatic fat infiltration,and a substantial accumulation of peritoneal fluid.Gastroscopy identified esophageal varices.Liver stiffness measurement indicated a value of 32.7 kPa.Based on the results of auxiliary examinations,common causes of cirrhosis were excluded,and a mutation in the Shwachman-Bodian-Diamond syndrome gene was ultimately identified through whole-exome sequencing.The patient was diagnosed with cirrhosis secondary to SDS.Following the correction of hypoalbuminemia and administration of diuretics,the patient's ascites resolved.CONCLUSION Patients with liver cirrhosis who also exhibit pancreatic fat infiltration and pancytopenia necessitate further exon testing to exclude the possibility of SDS.展开更多
BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increas...BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increase re-tear rates.AIM To investigate the potential inhibitory effects of alpha-tocopherol(vitamin E)and OTR-4131 on muscle atrophy,fatty infiltration,and fibrosis in rotator cuff muscles following a massive RCT using a Wistar rat model,and establish a standardized methodology for evaluating potential therapeutic agents.METHODS This protocol outlines a controlled animal study using 40 male Wistar rats,randomized into five groups.The experimental groups will receive either systemic administration of alpha-tocopherol or local administration of OTR-4131 via intramuscular injection into the supraspinatus and infraspinatus muscles.Two sham groups will receive systemic and local saline injections respectively,while a control group will undergo no intervention.The interventions will be administered after surgical transection of the supraspinatus and infraspinatus tendons.Outcomes will be assessed via wet muscle weight measurements,muscle fiber diameter,fatty infiltration percentage,and fibrosis evaluation using histological methods.RESULTS The study anticipates that alpha-tocopherol and OTR-4131 will reduce muscle atrophy,fatty infiltration,and fibrosis compared to control and sham groups,supporting their potential protective role in rotator cuff muscle degeneration.CONCLUSION The results are expected to improve the understanding on the role of alpha-tocopherol and OTR-4131 in rotator cuff muscle protection after massive RCT and may serve as a foundation for further preclinical and clinical research aimed at improving rotator cuff repair outcomes.展开更多
AIM:To investigate cuproptosis-related molecular and immune infiltration in age-related macular degeneration(AMD)development and establish a predictive model.METHODS:The expression profiles of cuproptosisrelated genes...AIM:To investigate cuproptosis-related molecular and immune infiltration in age-related macular degeneration(AMD)development and establish a predictive model.METHODS:The expression profiles of cuproptosisrelated genes and immune signature in AMD based on the microarray dataset GSE29801 were analyzed.A total of 142 AMD samples were used to identify the cuproptosisrelated differentially expressed genes(Cu-DEGs),together with the immune cell infiltration.To further refine the list of potential genes for AMD diagnosis,three machine learning techniques were used,and an external dataset were applied for confirming the accuracy of the predictive performance.Reverse transcription polymerase chain reaction(RT-PCR)were also performed to examine the level of mRNA of hub genes.The activated immune responses and Cu-DEGs were assessed between AMD and controls.RESULTS:Six genes,including ATP7A,DBT,VEGFA,UBE2D3,CP,SLC31A1,were screened as cuproptosissignature in AMD via three machine learning methods.Next,SLC31A1 and VEGFA was selected as hub genes by performance evaluation in an external dataset GSE160011,further analysis showed that SLC31A1 and VEGFA were associated with pathways related to immune signaling and immune function,which were then observed in relation to infiltrating immune cells.Finally,the mRNA expression levels of SLC31A1 and VEGFA were significantly higher in laser induced choroidal neovascularization(CNV)group than in control group detected by RT-PCR.CONCLUSION:In this study,the possible relationship between cuproptosis and AMD is expounded systematically.A predictive model is developed to assess the risk of cuproptosis-related genes and their clinical prognostic value in AMD patients.展开更多
Shallow-buried thick sand strata present considerable local instability risks during diaphragm wall trenching construction.However,this critical issue has not been extensively studied,despite its serious safety conseq...Shallow-buried thick sand strata present considerable local instability risks during diaphragm wall trenching construction.However,this critical issue has not been extensively studied,despite its serious safety consequences.This paper proposes an automatic identification model for shallow-buried thick sand strata,integrating three-dimensional limit equilibrium theory with a genetic algorithm to precisely identify the most potentially dangerous local instability mass and determine its minimum safety factor.The model establishes three undetermined parameters:failure angle,upper boundary,and thickness of the local instability mass.These parameters define the search space for the local instability mass.The effectiveness of this approach was confirmed through a diaphragm wall engineering case near the Rhine River in France,where the predicted instability location closely aligned with field observations.A systematic analysis of the model indicated that the difference in slurry-groundwater levels and the friction angle are the most significant factors affecting local instability in shallow-buried thick sand strata.The model indicated that the location of the most potentially dangerous instability mass changes depending on geological conditions,and larger instability masses do not always relate to lower safety factors.Additionally,exploratory experiments revealed that support pressure losses caused by slurry infiltration significantly influence local instability calculations in sand strata.This points out the importance of considering these support pressure losses in the stability evaluations of high permeable sand strata.The results improve the evaluation of safety and the optimization of design for diaphragm wall construction in shallow-buried thick sand strata.展开更多
Background:Rho GDP dissociation inhibitor 2(ARHGDIB),a key regulator of Rho GTPase,plays a significant role in the onset and progression of cancer by participating in various biological processes.However,the diverse b...Background:Rho GDP dissociation inhibitor 2(ARHGDIB),a key regulator of Rho GTPase,plays a significant role in the onset and progression of cancer by participating in various biological processes.However,the diverse biological roles of ARHGDIB across pan-cancer remain systematically and comprehensively unexplored.We aimed to elucidate the diagnostic and prognostic roles of ARHGDIB and its potential tumor-related mechanisms in human cancers,using bioinformatics approaches.Methods:Data on 33 tumor types were downloaded from The Cancer Genome Atlas,and R software was used to statistically analyze ARHGDIB expression levels and prognostic significance across pan-cancer.Western blot was used to verify the protein expression of ARHGDIB in breast cancer and liver cancer cell lines.Additionally,various databases,including UALCAN,GEPIA,cBioPortal,TIMER,CancerSEA,GSCALite,and GSEA,were used to examine ARHGDIB protein expression across pan-cancer,its correlation with tumor pathological staging,ARHGDIB mutation types and frequencies,and immune cell infiltration.Furthermore,functional analyses at the single-cell level,drug sensitivity assessments,and explorations of related signaling pathways were performed.Results:ARHGDIB exhibits abnormal expression at mRNA and protein levels across various cancers.Western blot results show that ARHGDIB is highly expressed in breast cancer and liver cancer cells.ARHGDIB influences the prognosis of lower-grade glioma,bladder cancer,sarcoma,and skin cutaneous melanoma.It exhibits the highest frequency of gene alterations in uterine carcinosarcoma,primarily characterized by gene amplification.Additionally,ARHGDIB is strongly associated with immune-infiltrating cells and immune checkpoints in the tumor microenvironment.It potentially influences cancer progression through multiple signaling pathways.Conclusions:ARHGDIB is a potential prognostic marker in various cancers and a crucial regulator of the tumor microenvironment.It represents a promising therapeutic target by modulating cancer progression through multiple biological behaviors and signaling pathways.展开更多
BACKGROUND Tumor-associated macrophages(TAMs)have demonstrated significant potential as a research and treatment approach for hepatocellular carcinoma(HCC).Nevertheless,a comprehensive quantitative analysis of TAMs in...BACKGROUND Tumor-associated macrophages(TAMs)have demonstrated significant potential as a research and treatment approach for hepatocellular carcinoma(HCC).Nevertheless,a comprehensive quantitative analysis of TAMs in HCC remained insufficient.Therefore,the objective of this study was to employ bibliometric methods to investigate the development trends and research frontiers pertaining to this field.AIM To determine the knowledge structure and current research hotspots by bibliometric analysis of scholarly papers pertaining to TAMs in HCC.METHODS The present study employed the Web of Science Core Collection to identify all papers related to TAMs in HCC research.Utilizing the Analysis Platform of Bibliometrics,CiteSpace 6.2.R4,and Vosviewer 1.6.19,the study conducted a comprehensive analysis encompassing multiple dimensions such as publication quantity,countries of origin,affiliated institutions,publishing journals,contributing authors,co-references,author keywords,and emerging frontiers within this research domain.RESULTS A thorough examination was undertaken on 818 papers within this particular field,published between January 1,1985 to September 1,2023,which has witnessed a substantial surge in scholarly contributions since 2012,with a notable outbreak in 2019.China was serving as the central hub in this field,with Fudan University leading in terms of publications and citations.Chinese scholars have taken the forefront in driving the research expansion within this field.Hepatology emerged as the most influential journal in this field.The study by Qian and Pollard in 2010 received the highest number of co-citations.It was observed that the citation bursts of references coincided with the outbreak of publications.Notably,“tumor microenvironment”,“immunotherapy”,“prognostic”,“inflammation”,and“polarization”,etc.emerged as frequently occurring keywords in this field.Of particular interest,“immune evasion”,“immune infiltration”,and“cancer genome atlas”were identified as emerging frontiers in recent research.CONCLUSION The field of TAMs in HCC exhibited considerable potential,as evidenced by the promising prospects of immunotherapeutic interventions targeting TAMs for the amelioration of HCC.The emerging frontiers in this field primarily revolved around modulating the immunosuppressive characteristics of TAMs within a liver-specific immune environment,with a focus on how to counter immune evasion and reduce immune infiltration.展开更多
BACKGROUND Colorectal cancer(CRC)remains a major global health burden due to its high incidence and mortality,with treatment efficacy often hindered by tumor hetero-geneity,drug resistance,and a complex tumor microenv...BACKGROUND Colorectal cancer(CRC)remains a major global health burden due to its high incidence and mortality,with treatment efficacy often hindered by tumor hetero-geneity,drug resistance,and a complex tumor microenvironment(TME).Lactate metabolism plays a pivotal role in reshaping the TME,promoting immune eva-sion and epithelial-mesenchymal transition,making it a promising target for novel therapeutic strategies and prognostic modeling in CRC.AIM To offer an in-depth analysis of the role of lactate metabolism in CRC,high-lighting its significance in the TME and therapeutic response.METHODS Utilizing single-cell and transcriptomic data from the Gene Expression Omnibus and The Cancer Genome Atlas,we identified key lactate metabolic activities,particularly in the monocyte/macrophage subpopulation.RESULTS Seven lactate metabolism-associated genes were significantly linked to CRC prognosis and used to construct a predictive model.This model accurately forecasts patient outcomes and reveals notable distinct patterns of immune infiltration and transcriptomic profiles mutation profiles between high-and low-risk groups.High-risk patients demonstrated elevated immune cell infiltration,increased mutation frequencies,and heightened sensitivity to specific drugs(AZD6482,tozasertib,and SB216763),providing a foundation for personalized treatment approaches.Additionally,a nomogram integrating clinical and metabolic data effectively predicted 1-,3-,and 5-year survival rates.CONCLUSION This report underscored the pivotal mechanism of lactate metabolism in CRC prognosis and suggest novel avenues for therapeutic intervention.展开更多
The Mg−1Zn−1Sn and Mg−1Zn−1Sn−0.2Ca alloy scaffolds were prepared via infiltration casting using 3D-printed Ti templates to achieve complete and accurate control of the pore structure.The results indicate that the act...The Mg−1Zn−1Sn and Mg−1Zn−1Sn−0.2Ca alloy scaffolds were prepared via infiltration casting using 3D-printed Ti templates to achieve complete and accurate control of the pore structure.The results indicate that the actual porosity and pore size of the prepared P model for each pore size are greater than the designed values.The addition of Ca changes the second phase of the alloy from Mg_(2)Sn to CaMgSn and refines its microstructure.The compressive yield strength and compressive modulus of the Mg−1Zn−1Sn−0.2Ca alloy scaffold reach 32.61 MPa and 0.23 GPa,respectively.The corrosion current density is measured at 14.64μA/cm^(2),with an instantaneous corrosion rate of 0.335 mm/a.Both scaffolds exhibit excellent biocompatibility and no cytotoxicity.Additionally,the antibacterial effects of both alloys on E.coli are greater than 97.81%.These results indicate that Mg alloy scaffolds have great potential for clinical applications.展开更多
Understanding the infiltration process and quantifying recharge are critical for effective water resources management,particularly in arid and semi-arid regions.However,factors influencing on recharge process under di...Understanding the infiltration process and quantifying recharge are critical for effective water resources management,particularly in arid and semi-arid regions.However,factors influencing on recharge process under different land use types in irrigation districts remain unclear.In this study,a Brilliant Blue FCF dye tracer experiment was conducted to investigate infiltration pathways under the cotton field,pear orchard,and bare land conditions in the Kongque Rive Irrigation District of Xinjiang,China.Recharge rates were estimated using the chloride mass balance method.The results show that the average preferential flow ratio was highest in the bare land(50.42%),followed by the cotton field(30.09%)and pear orchard(23.59%).Matrix flow was the dominant infiltration pathway in the pear orchard and cotton field.Irrigation method was a primary factor influencing recharge rates,with surface irrigation promoting deeper infiltration compared to drip irrigation.Under the drip irrigation mode,the recharge of cotton fields ranged from 23.47 mm/a to 59.16 mm/a.In comparison,the recharge of surface irrigation in pear orchards contributed between 154.30 mm/a and 401.65 mm/a.These findings provide valuable insights into soil water infiltration and recharge processes under typical land use conditions in the Kongque River Irrigation District,supporting improved irrigation management and sustainable water resource utilization.展开更多
文摘Introduction/Purpose: The “transverse abdominal plane block” or TAP block was described by Rafi in 2001. It describes an approach to the neurofascial plane of the transverse abdominal muscle via the Jean-Louis Petit triangle and provides analgesia of the entire homolateral hemi abdomen. The aim of our study was to compare post-operative analgesia and post-operative morphine requirements between transverse abdominal plane block (TAP) and peri-orificial infiltration during laparoscopic abdominal surgery. Material and method: Prospective, randomized study conducted over a 2-year period. The study was conducted in the operating theatre of the Saint Louis Regional Hospital in Senegal. All adult patients undergoing laparoscopic abdominal surgery were included. Clinical aspects, pain scales and morphine consumption were analyzed. Results: A total of 60 patients were enrolled: 30 patients in the TAP group and 30 patients in the infiltration group. The average age was 32.9 years. The indications for laparoscopy were acute appendicitis in 50% of cases, gallbladder stones in 16% and inguinal hernia in 8%. For the TAP group, the mean numerical pain scale was 3.9 at 6 hours post-operatively and 2.1 at 24 hours post-operatively. For the infiltration group, the mean numerical pain scale was 4.3 at 6 hours post-op and 3 at 24 hours post-op. Morphine consumption at 6 hours post-op was on average 0.4 mg/patient for the TAP group and 0.9 mg/patient for the infiltration group. Discussion/conclusion: Analgesia provided by ultrasound-guided TAP block for laparoscopic abdominal surgery appears to be identical to periorificial infiltration. However, the simplicity and reproducibility of ultrasound-guided TAP block gives it a definite advantage.
基金supported by the NationalNatural Science Foundation of China(no.32360888)the Jiangxi Students’Platform for Innovation and Entrepreneurship Training Program(no.202411843023).
文摘Background:Heat shock protein B8(HSPB8)is implicated in autophagy,and its aberrant expression has been linked to both the ini-tiation and progression of tumors.However,the role and function of HSPB8 in colorectal cancer(CRC)and across multiple cancer types remain unclear.This study aimed to map the transcriptome of autophagy-related genes in CRC and to conduct a pan-cancer analysis of HSPB8 as both a prognostic and immunological biomarker.Methods:We performed bioinformatics analyses on GSE113513 and GSE74602 to identify differentially expressed genes(DEGs)in CRC.These DEGs were then compared with autophagy-related genes to identify critical overlapping genes.The Kaplan-Meier plotter was used to verify the ex-pression of autophagy-linked DEGs and evaluate its prognostic value.The protein expression of Hub gene in CRC was analyzed using the Human Protein Atlas database.The cBioPortal was used to analyze the type and frequency of Hub gene mutations.The TIMER(Tumor Immune Estimation Resource)database was used to study the correlation between HSPB8 and immune infiltration in CRC.Results:In total,825 DEGs were identified,including 8 autophagy-linked DEGs:ATIC,MYC,HSPB8,TNFSF10,BCL2,TP53INP2,ITPR1,and NKX2-3.Survival analysis showed that increased HSPB8 expression significantly correlates with poor prognosis in patients with CRC(p<0.05).HSPB8 was also found to be differentially expressed in various cancer types,correlating with both prognosis and immune infiltration.Further,changes in HSPB8 methylation and phosphorylation status were observed across several cancers,suggesting potential regulatory mechanisms.Therefore,HSPB8 may serve as a crucial prognostic and immunological biomarker in CRC and other cancers.Conclusions:This study provides new insights into the role of autophagy-related genes in cancer progression and highlights HSPB8 as a potential target for cancer diagnostics and therapy.
基金supported by the National Natural Science Foundation of China(Grant Nos.52275321 and 52205348)the Shandong Natural Science Foundation(Grant No.ZR2023JQ021)+3 种基金the Taishan Scholars Foundation of Shandong Province(No.tsqn 201812128)the Innovation Scientists and Technicians Troop Projects of Henan Province(No.204200510031)the Heilongjiang Touyan Innovation Team Program(No.HITTY-20190013)supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIP)(Nos.NRF-2021R1A2C3006662 and NRF-2022R1A5A1030054).
文摘To effectively regulate the grain boundary infiltration in CoCrFeMnNi high-entropy alloy(Cantor alloys,HEA)caused by the violent atomic interdiffusion,the higher configuration entropy on Cantor alloys surface was designed and realized via eutectic high-entropy(EHEA)transformation.Meanwhile,to effectively alleviate the residual stress caused by the notable difference in the thermal expansion coefficient(CTE)between Cantor alloys and Zr-3 alloys,a cladding layer was applied to the HEA surface using laser cladding technology of Nb,followed by brazing to Zr-3 alloys with Zr63.2Cu filler.The cladding layer’s microstructure comprised Nbss and FCC+(Co,Ni)_(2) Nb eutectic structure,resulting from an in-situ reaction between Cantor alloys and Nb.The Nbss and FCC demonstrated good plasticity,and the(Co,Ni)_(2) Nb Laves phase provided increased strength,endowing both good plastic deformation ability and strength of the cladding layer.Notably,the existence of EHEA in the laser cladding layer made the Cantor alloy entropy from 1.61 R to 1.77 R,greatly enhancing its thermal stability and suppressing the grave grain boundary infiltration.Joints produced via laser cladding with Nb-assisted brazing exhibited a complex microstructure(HEA/Nbss+FCC+(Co,Ni)_(2)Nb/(Zr,Nb)(Cr,Mn)_(2)+(Zr,Nb)ss/(Zr,Nb)_(2)(Cu,Ni,Co,Fe)+(Zr,Nb)(Cr,Mn)_(2)+(Zr,Nb)ss/Zr-3) and a significantly improved shear strength of 242.8 MPa at 1010℃ for 10 min,42.4%higher than that of directly brazed joints.This improvement was attributed to reduced grain boundary infiltration,alleviated residual stress due to CTE disparity,and eliminated micro-cracks in the brazing seam.This study presents an effective solution for reducing residual stresses and achieving reliable bonding between Cantor alloys and Zr-3 alloys,with potential applications in brazing CoCrFeNi-based HEA and Zr-3 due to the beneficial eutectic reaction between CoCrFeNi and Nb.
基金Supported by Joint Project on Regional High Incidence Diseases Research of Guangxi Natural Science Foundation,No.2024GXNSFAA010057 and No.2024GXNSFAA010085Natural Science Foundation of Guangxi,China,No.2022GXNSFBA035657+2 种基金Guangxi Zhuang Autonomous Region Health Commission Self-Financed Scientific Research Project,No.Z20210764Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Scientific Research Project,No.GXZYA20230270 and No.GXZYA20240305Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University(2022).
文摘BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.
基金supported by grants from the Weifang Municipal Health Commission Research Programme Project(No.WFWSJK-2023-032)the Weifang Science and Technology Development Project(No.2023YX053)the Weifang Young Medical Talent Support Program Funding。
文摘Background:NDC80 is pivotal in cell division,particularly in regulating the G2/M transition and mitotic progression.Recent studies have demonstrated that NDC80 is significantly overexpressed in multiple solid tumors.Further analysis has suggested that its high expression is significantly associated with an elevated pathological grade,increased metastatic risk,and shortened overall survival in patients with cancer.However,its precise role in pan-cancer development,progression,and prognosis remains unclear.Methods:We conducted a multi-omics analysis of NDC80 using genomic,transcriptomic,and proteomic data from 33 cancer types in The Cancer Genome Atlas,Clinical Proteomic Tumor Analysis Consortium,Genotype-Tissue Expression,and Human Protein Atlas.Results:The results demonstrated frequent NDC80 mutations across multiple malignancies and significantly elevated expression in tumor tissues compared with that in their normal counterparts,correlating with worse overall and disease-free survival.Moreover,NDC80 expression was strongly associated with oncogenic pathways,key protein regulators,cellular components,myeloid-derived suppressor cell infiltration,ESTIMATE scores,and cancer-related signaling networks.Conclusions:These findings underscore the potential of NDC80 as a prognostic biomarker and therapeutic target for cancer treatment.
文摘To the Editor:Pancreatic cancer is a malignancy characterized by a poor prog-nosis,with a 5-year survival rate of<10%[1].Furthermore,only a minority of patients(<20%)qualify for curative-intent resec-tion,and even among those who undergo this procedure,the risk of recurrence within three years remains alarmingly high,reach-ing up to 70%[2].Due to the lack of specific clinical manifes-tations of pancreatic cancer,most cases have metastasized or in-vaded the major vessels around the pancreas at the time of initial diagnosis,resulting in a low surgical resection rate.Even patients who undergo surgical resection often face a poor prognosis[3].In recent years,neoadjuvant chemotherapy using agents such as gemcitabine,5-fluorouracil,albumin-bound paclitaxel,modified fluorouracil/leucovorin plus irinotecan,and oxaliplatin(mFOLFIRI-NOX),targeted therapies addressing molecular subtypes of pan-creatic cancer,and immunotherapies targeting PD-1 and PD-L1 have shown efficacy in improving the overall prognosis of patients with pancreatic cancer,although the impact remains modest[4,5].Therefore,novel therapeutic strategies and prognostic evaluation systems are urgently needed to enhance the survival of patients with pancreatic cancer.
文摘Empirical formulas are indispensable tools in water engineering and hydraulic structure design.Derived from meticulous field observations,experiments,and diverse datasets,these formulas help to estimate water leakage in structures such as dams,tunnels,canals,and pipelines.By utilizing a few easily measurable parameters,engineers can employ these formulas to generate preliminary leakage rate estimates before proceeding with more detailed analyses.In this study,a physical model was developed,and a series of experiments were conducted,considering variables such as inflow rate,materials constituting the unsaturated medium,and variations in infiltration trench depth and width.As a result,a novel artificial recharge method was introduced,and an empirical equation,Q_(out)=0.0066×D_(50)^(0.64)×L×P^(0.36),was proposed to estimate the infiltration capacity of the trench.This equation incorporates factors such as the wetted perimeter,mean soil particle diameter,trench length,and a coefficient.A comparative analysis between the observed data from nine Iranian earthen canals and the values calculated using the proposed equation revealed an average relative error of 15%between the two datasets.In addition,the Pearson correlation coefficient was determined to be 0.981 and the Root Mean Square Error(RMSE)was 0.381,demonstrating the strong predictive performance of the equation.The parameters considered in the proposed equation allow for its application across diverse regions.Given its accurate performance,this equation provides a reliable initial estimate of the leakage rate,thereby helping to reduce costs and save time.
文摘BACKGROUND Acute liver failure(ALF)due to diffuse hepatic infiltration by metastatic mela-noma is extremely rare and often misdiagnosed.In the absence of prior malig-nancy,this presentation can mimic other hepatic emergencies such as Budd-Chiari syndrome.Early identification is crucial,especially in transplant candidates,to prevent inappropriate management.CASE SUMMARY A 61-year-old male presented with jaundice,abdominal distension,and enceph-alopathy.Liver imaging suggested acute Budd-Chiari syndrome,and liver trans-plantation was considered.However,biopsy revealed extensive hepatic infilt-ration by human melanoma black-positive melanoma cells.There was no known can-cer history,although retrospective symptoms suggested uveal localization as a possi-ble primary site.The patient rapidly deteriorated and died.A review of 12 simi-lar cases revealed shared diagnostic challenges,frequent misdiagnoses,and poor outcomes.CONCLUSION Infiltrative melanoma should be considered in unexplained ALF,even without previously known malignancy.
基金supported by the Natural Science Foundation of Anhui Province(ML 2308085MC80)the Anhui Medical University Research and Innovation Talent Team(KZ).
文摘Objectives:Non-small cell lung cancer(NSCLC)represents a formidable malignancy characterized by its marked metastatic potential and intrinsic resistance to therapeutic interventions.The identification of potential biomarkers delineating the progression and metastatic cascade of NSCLC assumes paramount importance in fostering advancements toward enhanced patient outcomes and prognostic stratification.Methods:The expression level of the actin-related protein 2/3 complex;subunit 1A(ARPC1A)in NSCLC was evaluated using The Cancer Genome Atlas(TCGA)and Gene Expression Profiling Interactive Analysis(GEPIA)databases;along with the LinkedOmics database for co-expression genes.Further verification of ARPC1A expression in normal lung cells and NSCLC cells;as well as in normal tissues and lung cancer tissues;was performed using quantitative real-time reverse transcription PCR(RTqPCR)and Western blotting.The function of ARPC1A was explored through Gene Set Enrichment Analysis(GSEA)and immune infiltration analysis;followed by functional experiments for validation.Results:ARPC1A is upregulated in NSCLC and is associated with unfavorable clinical prognoses.Additionally,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis highlights a potential link between the ARPC1A gene and the cell cycle and p53 signaling pathways.ARPC1A also promotes cell proliferation and resistance to chemotherapeutic drugs,thereby enhancing the oncogenic potential of NSCLC.Relevant cell-based experiments confirm that targeted inhibition of ARPC1A effectively suppresses cellular migratory and invasive capabilities.The immune infiltration analysis showed a close association between ARPC1A expression and various immune components,suggesting ARPC1A may interact with the tumor microenvironment.Mechanistically,ARPC1A promotes cell migration by stimulating the epithelialto-mesenchymal transition(EMT).Conclusion:The study results revealed the potential of ARPC1A as a valuable prognostic biomarker for NSCLC.Additionally,the associated mechanisms provide insights that may pave the way for therapeutic interventions for NSCLC patients.
文摘BACKGROUND Shwachman-Diamond syndrome(SDS)is a rare genetic disorder that affects multiple organs,primarily the liver.Most patients are diagnosed during infancy or early childhood.As they grow older,the majority of affected children may experience spontaneous remission,and cases of cirrhosis in adults are rarely reported.CASE SUMMARY A 36-year-old male patient presented with massive ascites.Laboratory tests revealed pancytopenia and a serum-ascites albumin gradient greater than 1.1 g/dL.An abdominal computed tomography scan demonstrated cirrhosis,splenomegaly,pancreatic fat infiltration,and a substantial accumulation of peritoneal fluid.Gastroscopy identified esophageal varices.Liver stiffness measurement indicated a value of 32.7 kPa.Based on the results of auxiliary examinations,common causes of cirrhosis were excluded,and a mutation in the Shwachman-Bodian-Diamond syndrome gene was ultimately identified through whole-exome sequencing.The patient was diagnosed with cirrhosis secondary to SDS.Following the correction of hypoalbuminemia and administration of diuretics,the patient's ascites resolved.CONCLUSION Patients with liver cirrhosis who also exhibit pancreatic fat infiltration and pancytopenia necessitate further exon testing to exclude the possibility of SDS.
基金thank the staff of the accredited animal facility of the laboratory of anatomy,Histology and Embryology of Aristotle University of Thessaloniki’s veterinary school for their assistance in animal handling and care.
文摘BACKGROUND Massive rotator cuff tears(RCTs)result in impaired shoulder function and quality of life.These tears lead to structural changes in the rotator cuff muscles,which compromise recovery after repair and increase re-tear rates.AIM To investigate the potential inhibitory effects of alpha-tocopherol(vitamin E)and OTR-4131 on muscle atrophy,fatty infiltration,and fibrosis in rotator cuff muscles following a massive RCT using a Wistar rat model,and establish a standardized methodology for evaluating potential therapeutic agents.METHODS This protocol outlines a controlled animal study using 40 male Wistar rats,randomized into five groups.The experimental groups will receive either systemic administration of alpha-tocopherol or local administration of OTR-4131 via intramuscular injection into the supraspinatus and infraspinatus muscles.Two sham groups will receive systemic and local saline injections respectively,while a control group will undergo no intervention.The interventions will be administered after surgical transection of the supraspinatus and infraspinatus tendons.Outcomes will be assessed via wet muscle weight measurements,muscle fiber diameter,fatty infiltration percentage,and fibrosis evaluation using histological methods.RESULTS The study anticipates that alpha-tocopherol and OTR-4131 will reduce muscle atrophy,fatty infiltration,and fibrosis compared to control and sham groups,supporting their potential protective role in rotator cuff muscle degeneration.CONCLUSION The results are expected to improve the understanding on the role of alpha-tocopherol and OTR-4131 in rotator cuff muscle protection after massive RCT and may serve as a foundation for further preclinical and clinical research aimed at improving rotator cuff repair outcomes.
文摘AIM:To investigate cuproptosis-related molecular and immune infiltration in age-related macular degeneration(AMD)development and establish a predictive model.METHODS:The expression profiles of cuproptosisrelated genes and immune signature in AMD based on the microarray dataset GSE29801 were analyzed.A total of 142 AMD samples were used to identify the cuproptosisrelated differentially expressed genes(Cu-DEGs),together with the immune cell infiltration.To further refine the list of potential genes for AMD diagnosis,three machine learning techniques were used,and an external dataset were applied for confirming the accuracy of the predictive performance.Reverse transcription polymerase chain reaction(RT-PCR)were also performed to examine the level of mRNA of hub genes.The activated immune responses and Cu-DEGs were assessed between AMD and controls.RESULTS:Six genes,including ATP7A,DBT,VEGFA,UBE2D3,CP,SLC31A1,were screened as cuproptosissignature in AMD via three machine learning methods.Next,SLC31A1 and VEGFA was selected as hub genes by performance evaluation in an external dataset GSE160011,further analysis showed that SLC31A1 and VEGFA were associated with pathways related to immune signaling and immune function,which were then observed in relation to infiltrating immune cells.Finally,the mRNA expression levels of SLC31A1 and VEGFA were significantly higher in laser induced choroidal neovascularization(CNV)group than in control group detected by RT-PCR.CONCLUSION:In this study,the possible relationship between cuproptosis and AMD is expounded systematically.A predictive model is developed to assess the risk of cuproptosis-related genes and their clinical prognostic value in AMD patients.
基金supported by the Fundamental Research Funds for the Central Universities(No.02302350113).
文摘Shallow-buried thick sand strata present considerable local instability risks during diaphragm wall trenching construction.However,this critical issue has not been extensively studied,despite its serious safety consequences.This paper proposes an automatic identification model for shallow-buried thick sand strata,integrating three-dimensional limit equilibrium theory with a genetic algorithm to precisely identify the most potentially dangerous local instability mass and determine its minimum safety factor.The model establishes three undetermined parameters:failure angle,upper boundary,and thickness of the local instability mass.These parameters define the search space for the local instability mass.The effectiveness of this approach was confirmed through a diaphragm wall engineering case near the Rhine River in France,where the predicted instability location closely aligned with field observations.A systematic analysis of the model indicated that the difference in slurry-groundwater levels and the friction angle are the most significant factors affecting local instability in shallow-buried thick sand strata.The model indicated that the location of the most potentially dangerous instability mass changes depending on geological conditions,and larger instability masses do not always relate to lower safety factors.Additionally,exploratory experiments revealed that support pressure losses caused by slurry infiltration significantly influence local instability calculations in sand strata.This points out the importance of considering these support pressure losses in the stability evaluations of high permeable sand strata.The results improve the evaluation of safety and the optimization of design for diaphragm wall construction in shallow-buried thick sand strata.
基金supported by a grant from the Hefei Municipal Health Commission Applied Medical Research Project(No.Hwk2023zd007)。
文摘Background:Rho GDP dissociation inhibitor 2(ARHGDIB),a key regulator of Rho GTPase,plays a significant role in the onset and progression of cancer by participating in various biological processes.However,the diverse biological roles of ARHGDIB across pan-cancer remain systematically and comprehensively unexplored.We aimed to elucidate the diagnostic and prognostic roles of ARHGDIB and its potential tumor-related mechanisms in human cancers,using bioinformatics approaches.Methods:Data on 33 tumor types were downloaded from The Cancer Genome Atlas,and R software was used to statistically analyze ARHGDIB expression levels and prognostic significance across pan-cancer.Western blot was used to verify the protein expression of ARHGDIB in breast cancer and liver cancer cell lines.Additionally,various databases,including UALCAN,GEPIA,cBioPortal,TIMER,CancerSEA,GSCALite,and GSEA,were used to examine ARHGDIB protein expression across pan-cancer,its correlation with tumor pathological staging,ARHGDIB mutation types and frequencies,and immune cell infiltration.Furthermore,functional analyses at the single-cell level,drug sensitivity assessments,and explorations of related signaling pathways were performed.Results:ARHGDIB exhibits abnormal expression at mRNA and protein levels across various cancers.Western blot results show that ARHGDIB is highly expressed in breast cancer and liver cancer cells.ARHGDIB influences the prognosis of lower-grade glioma,bladder cancer,sarcoma,and skin cutaneous melanoma.It exhibits the highest frequency of gene alterations in uterine carcinosarcoma,primarily characterized by gene amplification.Additionally,ARHGDIB is strongly associated with immune-infiltrating cells and immune checkpoints in the tumor microenvironment.It potentially influences cancer progression through multiple signaling pathways.Conclusions:ARHGDIB is a potential prognostic marker in various cancers and a crucial regulator of the tumor microenvironment.It represents a promising therapeutic target by modulating cancer progression through multiple biological behaviors and signaling pathways.
基金Supported by the Sanming Project of Medicine in Shenzhen,No.SZZYSM202111002Shenzhen Medical Research Fund,No.B2302008+1 种基金Shenzhen Science and Technology Program,No.JCYJ20220531091809022,No.JSGG20210802093208023,No.JCYJ20220818103402006,and No.ZDSYS201606081515458Traditional Chinese Medicine Bureau of Guangdong Province,No.20231286.
文摘BACKGROUND Tumor-associated macrophages(TAMs)have demonstrated significant potential as a research and treatment approach for hepatocellular carcinoma(HCC).Nevertheless,a comprehensive quantitative analysis of TAMs in HCC remained insufficient.Therefore,the objective of this study was to employ bibliometric methods to investigate the development trends and research frontiers pertaining to this field.AIM To determine the knowledge structure and current research hotspots by bibliometric analysis of scholarly papers pertaining to TAMs in HCC.METHODS The present study employed the Web of Science Core Collection to identify all papers related to TAMs in HCC research.Utilizing the Analysis Platform of Bibliometrics,CiteSpace 6.2.R4,and Vosviewer 1.6.19,the study conducted a comprehensive analysis encompassing multiple dimensions such as publication quantity,countries of origin,affiliated institutions,publishing journals,contributing authors,co-references,author keywords,and emerging frontiers within this research domain.RESULTS A thorough examination was undertaken on 818 papers within this particular field,published between January 1,1985 to September 1,2023,which has witnessed a substantial surge in scholarly contributions since 2012,with a notable outbreak in 2019.China was serving as the central hub in this field,with Fudan University leading in terms of publications and citations.Chinese scholars have taken the forefront in driving the research expansion within this field.Hepatology emerged as the most influential journal in this field.The study by Qian and Pollard in 2010 received the highest number of co-citations.It was observed that the citation bursts of references coincided with the outbreak of publications.Notably,“tumor microenvironment”,“immunotherapy”,“prognostic”,“inflammation”,and“polarization”,etc.emerged as frequently occurring keywords in this field.Of particular interest,“immune evasion”,“immune infiltration”,and“cancer genome atlas”were identified as emerging frontiers in recent research.CONCLUSION The field of TAMs in HCC exhibited considerable potential,as evidenced by the promising prospects of immunotherapeutic interventions targeting TAMs for the amelioration of HCC.The emerging frontiers in this field primarily revolved around modulating the immunosuppressive characteristics of TAMs within a liver-specific immune environment,with a focus on how to counter immune evasion and reduce immune infiltration.
基金Supported by Henan Province Science and Technology Research Project,No.232102310043Henan Provincial Science and Technology Research and Development Plan Joint Fund,No.222103810047Key Scientific Research Project Plan of Colleges and Universities in Henan Province,No.22A320033.
文摘BACKGROUND Colorectal cancer(CRC)remains a major global health burden due to its high incidence and mortality,with treatment efficacy often hindered by tumor hetero-geneity,drug resistance,and a complex tumor microenvironment(TME).Lactate metabolism plays a pivotal role in reshaping the TME,promoting immune eva-sion and epithelial-mesenchymal transition,making it a promising target for novel therapeutic strategies and prognostic modeling in CRC.AIM To offer an in-depth analysis of the role of lactate metabolism in CRC,high-lighting its significance in the TME and therapeutic response.METHODS Utilizing single-cell and transcriptomic data from the Gene Expression Omnibus and The Cancer Genome Atlas,we identified key lactate metabolic activities,particularly in the monocyte/macrophage subpopulation.RESULTS Seven lactate metabolism-associated genes were significantly linked to CRC prognosis and used to construct a predictive model.This model accurately forecasts patient outcomes and reveals notable distinct patterns of immune infiltration and transcriptomic profiles mutation profiles between high-and low-risk groups.High-risk patients demonstrated elevated immune cell infiltration,increased mutation frequencies,and heightened sensitivity to specific drugs(AZD6482,tozasertib,and SB216763),providing a foundation for personalized treatment approaches.Additionally,a nomogram integrating clinical and metabolic data effectively predicted 1-,3-,and 5-year survival rates.CONCLUSION This report underscored the pivotal mechanism of lactate metabolism in CRC prognosis and suggest novel avenues for therapeutic intervention.
基金the financial support for this work from the National Natural Science Foundation of China(Nos.52171241,52373251,52201301,51801137)Natural Science Foundation of Tianjin City,China(No.22JCQNJC00750)Tianjin University of Technology Graduate Research Innovation Project,China(No.YJ2235)。
文摘The Mg−1Zn−1Sn and Mg−1Zn−1Sn−0.2Ca alloy scaffolds were prepared via infiltration casting using 3D-printed Ti templates to achieve complete and accurate control of the pore structure.The results indicate that the actual porosity and pore size of the prepared P model for each pore size are greater than the designed values.The addition of Ca changes the second phase of the alloy from Mg_(2)Sn to CaMgSn and refines its microstructure.The compressive yield strength and compressive modulus of the Mg−1Zn−1Sn−0.2Ca alloy scaffold reach 32.61 MPa and 0.23 GPa,respectively.The corrosion current density is measured at 14.64μA/cm^(2),with an instantaneous corrosion rate of 0.335 mm/a.Both scaffolds exhibit excellent biocompatibility and no cytotoxicity.Additionally,the antibacterial effects of both alloys on E.coli are greater than 97.81%.These results indicate that Mg alloy scaffolds have great potential for clinical applications.
基金supported by the Hydrogeological Investigation Project in Kaidu River and Kongque River Basin in Xinjiang,China(No.DD2020171)the Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shaanxi Province(No.2020006).
文摘Understanding the infiltration process and quantifying recharge are critical for effective water resources management,particularly in arid and semi-arid regions.However,factors influencing on recharge process under different land use types in irrigation districts remain unclear.In this study,a Brilliant Blue FCF dye tracer experiment was conducted to investigate infiltration pathways under the cotton field,pear orchard,and bare land conditions in the Kongque Rive Irrigation District of Xinjiang,China.Recharge rates were estimated using the chloride mass balance method.The results show that the average preferential flow ratio was highest in the bare land(50.42%),followed by the cotton field(30.09%)and pear orchard(23.59%).Matrix flow was the dominant infiltration pathway in the pear orchard and cotton field.Irrigation method was a primary factor influencing recharge rates,with surface irrigation promoting deeper infiltration compared to drip irrigation.Under the drip irrigation mode,the recharge of cotton fields ranged from 23.47 mm/a to 59.16 mm/a.In comparison,the recharge of surface irrigation in pear orchards contributed between 154.30 mm/a and 401.65 mm/a.These findings provide valuable insights into soil water infiltration and recharge processes under typical land use conditions in the Kongque River Irrigation District,supporting improved irrigation management and sustainable water resource utilization.
