[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall o...[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.展开更多
[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous ...[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous Region. [ Method] The last instar larvae of Galleria mellonella and Tenebrio molitor were used as insect hosts. The infectivity of nematode was determined, and its heat tolerance ability was measured by using water bath method. [Result] The infectivity of the nematodes was lower at the dosages of 10, 20 and 40 IJs/larva, but higher at the dosages of 80,160, 32.0 and 640 IJs/larva. The corrected mortality （CM） of G. mellonella at 96 h and T. molitor at 120 h were both above 90%. The nematode infectivity was highest at the dosage of 80 IJs/larva when the hosts＇ CM reached 100% after 120 h. About 25% nematode individuals could survive in 36℃ water bath for 2 d in the densities of 1 000 -5 000 IJ/ml, after 6 d, there were still 3% -9% individuals survived, until 9 d the nematodes died totally. A hundred percent of the nematode individuals died in 38℃ and 40 ~C water bath after 6 h and 2 h, respectively. [Conclusion] Rhabditis （ O. ） sp. has a higher potential to cause the host mortality and represents heat tolerance up to 36℃.展开更多
基金Supported by Natural Science Foundation of China(31260461)Guangxi Innovation Team Nanning Test Station Project of National Modern Agriculture Technology System(nycytxcxtd-03-12-3,nycytxcxtd-04-18)Undergraduate Teaching Reform Project of Higher Education of Guangxi Province(2017JGB132)
文摘[Objectives] This study was conducted to analyze the infection ability of Rhizobium radiobacter from Rosa chinensis to peach tree,so as to provide a theoretical basis for the identification and control of crown gall of peach. [Methods]With peach variety ＂ Chiyohime＂ as an experimental material,R. radiobacter GOU1 was extracted from crown gall tissue of R. chinensis susceptible to crown gall,and its infection ability to peach tree was studied by in-vitro dip method.[Results]Stem segments of peach tree were inoculated with R. radiobacter GOU1 by in-vitro dip method. Calli grew rapidly after inoculation,and 28 d later,the infection rate of R. radiobacter GOU1 was 50%,indicating that R. radiobacter GOU1 could infect peach tree easier. The infection on stem segments of peach tree could be detected in the fourth week by in-vitro dip method. Culturing peach stem segments in MS medium containing 10 μM/L NAA and 10 μM/L BAP in vitro is beneficial to survival and could promote growth of callus. [Conclusions]The detection rate of R. radiobacter GOU1 infection on stem segments of peach tree did not change from the fourth week to the sixth week. Therefore,in future studies,the detection of R. radiobacter infection on inoculated stem segments of peach tree could be performed after 4 weeks of culture rather than 6 weeks of culture.
基金Supported by Ministry of Science and Technology of China on 863 Program"Soil Technique of Nematode Repair Biological Pollution"(2006AA06Z354)Support Plan Program"Production Technology ofHigh Efficiency Standard on Main Fruit Trees"(2008BAD92B08)BeijingGovernment on Purchasing Technology Service Program(2007DAg03B03-07)~~
文摘[ Objective] The study was aimed to determine the biological characteristics of Rhabditis （Oscheius） sp. isolated from a rhizosphere soil of alfalfa field in Hailar City, Hulunbuir League, Inner Mongolia Autonomous Region. [ Method] The last instar larvae of Galleria mellonella and Tenebrio molitor were used as insect hosts. The infectivity of nematode was determined, and its heat tolerance ability was measured by using water bath method. [Result] The infectivity of the nematodes was lower at the dosages of 10, 20 and 40 IJs/larva, but higher at the dosages of 80,160, 32.0 and 640 IJs/larva. The corrected mortality （CM） of G. mellonella at 96 h and T. molitor at 120 h were both above 90%. The nematode infectivity was highest at the dosage of 80 IJs/larva when the hosts＇ CM reached 100% after 120 h. About 25% nematode individuals could survive in 36℃ water bath for 2 d in the densities of 1 000 -5 000 IJ/ml, after 6 d, there were still 3% -9% individuals survived, until 9 d the nematodes died totally. A hundred percent of the nematode individuals died in 38℃ and 40 ~C water bath after 6 h and 2 h, respectively. [Conclusion] Rhabditis （ O. ） sp. has a higher potential to cause the host mortality and represents heat tolerance up to 36℃.
