Introduction:Lymphocytic choriomeningitis virus(LCMV)is a globally distributed zoonotic pathogen transmitted primarily by rodents.Its nonspecific clinical presentation in the general population frequently results in u...Introduction:Lymphocytic choriomeningitis virus(LCMV)is a globally distributed zoonotic pathogen transmitted primarily by rodents.Its nonspecific clinical presentation in the general population frequently results in underdiagnosis and underreporting.To date,no large-scale seroprevalence surveys of LCMV have been conducted in China.Methods:During 2023,this study collected 661 serum/plasma samples from healthy individuals and tested for anti-LCMV IgG antibodies using an enzyme-linked immunosorbent assay(ELISA).Samples tested positive by ELISA underwent confirmatory testing via indirect immunofluorescence analysis(IFA)and neutralization assay(NTA).Results:ELISA detected anti-LCMV IgG antibodies in 18.31%(121/661)of samples,while IFA confirmed seropositivity in 1.97%(13/661).Four samples demonstrated neutralizing antibody activity with reduced viral infectivity.Seropositive cases were sporadically distributed across the study region,with farmers representing the predominant occupational group.Key at-risk populations identified included farmers,rural residents,and individuals with lower educational attainment.Conclusion:This study provides the first serological evidence of past LCMV infections in humans in Gansu Province.Targeted health education interventions tailored to high-risk populations are recommended to reduce future transmission.展开更多
基金Supported by the Major Project of Guangzhou National Laboratory(Grant No.GZNL2024A01025)the Science and Technology Projects of Xizang Autonomous Region,China(Grant No.XZ202501JD0012).
文摘Introduction:Lymphocytic choriomeningitis virus(LCMV)is a globally distributed zoonotic pathogen transmitted primarily by rodents.Its nonspecific clinical presentation in the general population frequently results in underdiagnosis and underreporting.To date,no large-scale seroprevalence surveys of LCMV have been conducted in China.Methods:During 2023,this study collected 661 serum/plasma samples from healthy individuals and tested for anti-LCMV IgG antibodies using an enzyme-linked immunosorbent assay(ELISA).Samples tested positive by ELISA underwent confirmatory testing via indirect immunofluorescence analysis(IFA)and neutralization assay(NTA).Results:ELISA detected anti-LCMV IgG antibodies in 18.31%(121/661)of samples,while IFA confirmed seropositivity in 1.97%(13/661).Four samples demonstrated neutralizing antibody activity with reduced viral infectivity.Seropositive cases were sporadically distributed across the study region,with farmers representing the predominant occupational group.Key at-risk populations identified included farmers,rural residents,and individuals with lower educational attainment.Conclusion:This study provides the first serological evidence of past LCMV infections in humans in Gansu Province.Targeted health education interventions tailored to high-risk populations are recommended to reduce future transmission.
