Cliviaminiatais an ornamental plant with pharmaceutical potential,but its cultivation is costly.The plant’s slow growth has prompted the search for al-ternative cultivation methods aimed at producing phenolic compoun...Cliviaminiatais an ornamental plant with pharmaceutical potential,but its cultivation is costly.The plant’s slow growth has prompted the search for al-ternative cultivation methods aimed at producing phenolic compounds,such as theinvitroculture.This study investigated the effect of sucrose concentra-tion and fruit maturity on the invitrogermination(IG)of C.miniataseeds and subsequent callus induction(CI).Sucrose was added to the MS medium:0-40 g∙L^(-1).During IG,the concentration of sucrose affected the speed of ger-mination,and at 0 and 10 g∙L^(-1),seeds from ripe fruit had a higher germination percentage(63%and 67%).Mature seeds showed significantly higher percent-ages than immature ones.When inducing callus,a high concentration of growth regulators(GR)resulted in significant mortality of the explants.Com-binations such as 2.4-D mg∙L^(-1)+0.52 mg∙L^(-1)PIC+2 mg∙L^(-1)BAP and 4 mg∙L^(-1)BAP+4 mg∙L^(-1)ANA showed high survival rates and CI.The results demon-strate that sucrose concentrations>5.1 g∙L^(-1)reduce germination in mature seeds,likely due to osmotic stress,while lower doses(0-10 g∙L^(-1))optimize germination(up to 67%),indicating limited reliance on exogenous carbon sources.The cytokinin BAP proved to be essential for callogenesis,regardless of the auxin used.展开更多
文摘Cliviaminiatais an ornamental plant with pharmaceutical potential,but its cultivation is costly.The plant’s slow growth has prompted the search for al-ternative cultivation methods aimed at producing phenolic compounds,such as theinvitroculture.This study investigated the effect of sucrose concentra-tion and fruit maturity on the invitrogermination(IG)of C.miniataseeds and subsequent callus induction(CI).Sucrose was added to the MS medium:0-40 g∙L^(-1).During IG,the concentration of sucrose affected the speed of ger-mination,and at 0 and 10 g∙L^(-1),seeds from ripe fruit had a higher germination percentage(63%and 67%).Mature seeds showed significantly higher percent-ages than immature ones.When inducing callus,a high concentration of growth regulators(GR)resulted in significant mortality of the explants.Com-binations such as 2.4-D mg∙L^(-1)+0.52 mg∙L^(-1)PIC+2 mg∙L^(-1)BAP and 4 mg∙L^(-1)BAP+4 mg∙L^(-1)ANA showed high survival rates and CI.The results demon-strate that sucrose concentrations>5.1 g∙L^(-1)reduce germination in mature seeds,likely due to osmotic stress,while lower doses(0-10 g∙L^(-1))optimize germination(up to 67%),indicating limited reliance on exogenous carbon sources.The cytokinin BAP proved to be essential for callogenesis,regardless of the auxin used.
