期刊文献+
共找到2,436篇文章
< 1 2 122 >
每页显示 20 50 100
Exploration of the Application of Internet of Things Technology in Real-time Monitoring of Cold Chain Logistics
1
作者 Huiling Ma Xinyuan Liu +1 位作者 Weihan Zhao Haoyue Wu 《Journal of Electronic Research and Application》 2025年第3期165-170,共6页
The Internet of Things technology provides a comprehensive solution for the real-time monitoring of cold chain logistics by integrating sensors,wireless communication,cloud computing,and big data analysis.Based on thi... The Internet of Things technology provides a comprehensive solution for the real-time monitoring of cold chain logistics by integrating sensors,wireless communication,cloud computing,and big data analysis.Based on this,this paper deeply explores the overview and characteristics of the Internet of Things technology,the feasibility analysis of the Internet of Things technology in the cold chain logistics monitoring,the application analysis of the Internet of Things technology in the cold chain logistics real-time monitoring to better improve the management level and operational efficiency of the cold chain logistics,to provide consumers with safer and fresh products. 展开更多
关键词 Internet of Things technology Cold chain logistics real-time monitoring
在线阅读 下载PDF
Synthesis and luminescence in LiMgPO_4:Tb,Sm,B phosphors with possible applications in real-time dosimetry 被引量:8
2
作者 盖敏强 陈朝阳 +1 位作者 范艳伟 王军华 《Journal of Rare Earths》 SCIE EI CAS CSCD 2013年第6期551-554,共4页
The objective of this work was to develop possible materials for optically stimulated luminescence (OSL) dosimetric applications in real-time measurement. A novel material of LiMgPO4:Tb,Sm,B was prepared by solid-s... The objective of this work was to develop possible materials for optically stimulated luminescence (OSL) dosimetric applications in real-time measurement. A novel material of LiMgPO4:Tb,Sm,B was prepared by solid-state diffusion method at 900 oC. The structure and optical properties of these phosphors were characterized by X-ray diffraction, fluorescence spectro?photometer, and OSL reader. The results showed that the full discrimination between the stimulation and emission spectra made them very fit for the optic-fibre dosimetry. The OSL vs. dose response was linear in the dose range of 0.1 to 216 Gy. It also showed a significant improvement in the stimulation time compared with LiMgPO4:Tb,B. Hence, the phosphor could be used in the real-time dosimeter based on the OSL technology for medical monitoring as well as for environmental dosimetry and space dosimetry. 展开更多
关键词 DOSIMETRY LiMgPO4:Tb B optic-fibre OSL real-time rare earths
原文传递
A Class of Fast Algorithms in Real-Time Simulation 被引量:4
3
作者 Zhu Zhenmin & Liu Degui(Beijing Institute of Computer Application and Simulation Technology, 100854, P. R. China)Li Shoufu(Mathematics Department of Xiangtan University, 411105, P. R. China) 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 1999年第4期10-20,共11页
A class of hybrid algorithms of real-time simulation based on evaluation of non-integerstep right-hand side function are presented in this paper. And some results of the convergence and stability of the algorithms are... A class of hybrid algorithms of real-time simulation based on evaluation of non-integerstep right-hand side function are presented in this paper. And some results of the convergence and stability of the algorithms are given. Using the class of algorithms, evaluation for the right-hand side function is needed once in every integration-step. Moreover, comparing with the other methods with the same amount of work, their numerical stability regions are larger and the method errors are smaller, and the numerical experiments show that the algorithms are very effective. 展开更多
关键词 real-time simulation algorithm Numerical stability CONVERGENCE Dynamic system
在线阅读 下载PDF
Simulation of large-scale numerical substructure in real-time dynamic hybrid testing 被引量:9
4
作者 Zhu Fei Wang Jinting +2 位作者 Jin Feng Zhou Mengxia Gui Yao 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2014年第4期599-609,共11页
A solution scheme is proposed in this paper for an existing RTDHT system to simulate large-scale finite element (FE) numerical substructures. The analysis of the FE numerical substructure is split into response anal... A solution scheme is proposed in this paper for an existing RTDHT system to simulate large-scale finite element (FE) numerical substructures. The analysis of the FE numerical substructure is split into response analysis and signal generation tasks, and executed in two different target computers in real-time. One target computer implements the response analysis task, wherein a large time-step is used to solve the FE substructure, and another target computer implements the signal generation task, wherein an interpolation program is used to generate control signals in a small time-step to meet the input demand of the controller. By using this strategy, the scale of the FE numerical substructure simulation may be increased significantly. The proposed scheme is initially verified by two FE numerical substructure models with 98 and 1240 degrees of freedom (DOFs). Thereafter, RTDHTs of a single frame-foundation structure are implemented where the foundation, considered as the numerical substructure, is simulated by the FE model with 1240 DOFs. Good agreements between the results of the RTDHT and those from the FE analysis in ABAQUS are obtained. 展开更多
关键词 real-time dynamic hybrid testing large-scale numerical substructure control signal generation finite element simulation
在线阅读 下载PDF
Electrochemical drilling of small holes by regulating in real-time the electrolyte flowrate in multiple channels 被引量:1
5
作者 Jinxing LUO Xiaolong FANG +1 位作者 Tao YANG Di ZHU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2022年第5期470-483,共14页
Electrochemical drilling(ECD)provides an alternative technique for drilling multiple small holes in difficult-to-machine materials in numerous industrial applications such as for aeroengines.The value and fluctuation ... Electrochemical drilling(ECD)provides an alternative technique for drilling multiple small holes in difficult-to-machine materials in numerous industrial applications such as for aeroengines.The value and fluctuation of electrolyte flowrate can seriously affect the machining stability and hole quality in ECD.In particular,when drilling multiple holes,the distribution and fluctuations of the electrolyte flowrate in each channel could influence the uniformity of the electrolyte flowrate among multiple tube electrodes,thereby affecting the machining stability and the maximum feed rate.Thus,an eight-channel flow control system was developed to measure and regulate in real time the electrolyte flowrate supplied into each individual tube electrode.This paper proposes ECD of small holes with real-time flowrate control to improve the uniformity of electrolyte flowrate in each tube electrode and reduce the fluctuations in the electrolyte flowrate.In single-hole drilling,when the electrolyte flowrate was regulated in real time,the hole quality and machining stability were considerably better than without regulation.This is because the electrolyte flowrate remains basically constant,which stabilizes the flow field.Moreover,by considering the hole profile,it was found that an electrolyte flowrate of 200.0 m L/min can be acceptable for ECD of small holes.When the eight-channel flow control system was used in multiple-hole drilling,the uniformity of the electrolyte flowrate in each tube electrode was obviously improved,which led to a more stable process.Additionally,the maximum feed rate can attain 2.40 mm/min in multiple-hole drilling.Based on these findings,a matrix(5×32)of multiple small holes was successfully fabricated with a satisfactory diameter consistency,as the machining stability and machining efficiency had been enhanced. 展开更多
关键词 Electrochemical drilling Small holes Electrolyte flowrate real-time regulation Machining stability
原文传递
Exploration of instantaneous frequency for local control assessment in real-time hybrid simulation 被引量:1
6
作者 Xu Weijie Peng Changle +1 位作者 Guo Tong Chen Cheng 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第4期995-1008,共14页
Local control parameters such as instantaneous delay and instantaneous amplitude play an essential role in evaluating the performance and maintaining the stability of real-time hybrid simulation(RTHS).However,existing... Local control parameters such as instantaneous delay and instantaneous amplitude play an essential role in evaluating the performance and maintaining the stability of real-time hybrid simulation(RTHS).However,existing methods have limitations in obtaining this local assessment in either the time domain or frequency domain.In this study,the instantaneous frequency is introduced to determine local control parameters for actuator tracking assessment in a real-time hybrid simulation.Instantaneous properties,including amplitude,delay,frequency and phase,are then calculated based on analytic signals translated from actuator tracking signals through the Hilbert transform.Potential issues are discussed and solutions are proposed for calculation of local control parameters.Numerical simulations are first conducted for sinusoidal and chirp signals with time varying amplitude error and delay to demonstrate the potential of the proposed method.Laboratory tests also are conducted for a predefined random signal as well as the RTHS of a single degree of freedom structure with a self-centering viscous damper to experimentally verify the effectiveness of the proposed use of the instantaneous frequency.Results from the ensuing analysis clearly demonstrate that the instantaneous frequency provides great potential for local control assessment,and the proposed method enables local tracking parameters with good accuracy. 展开更多
关键词 real-time hybrid simulation tracking assessment instantaneous frequency Hilbert transform
在线阅读 下载PDF
A multifunctional shear apparatus for rocks subjected to true triaxial stress and high temperature in real-time 被引量:1
7
作者 Jun Zhao Xia-Ting Feng +2 位作者 Jia-Rong Wang Liang Hu Yue Guo 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第9期3524-3543,共20页
Deep engineering disasters,such as rockbursts and collapses,are more related to the shear slip of rock joints.A novel multifunctional device was developed to study the shear failure mechanism in rocks.Using this devic... Deep engineering disasters,such as rockbursts and collapses,are more related to the shear slip of rock joints.A novel multifunctional device was developed to study the shear failure mechanism in rocks.Using this device,the complete shearedeformation process and long-term shear creep tests could be performed on rocks under constant normal stiffness(CNS)or constant normal loading(CNL)conditions in real-time at high temperature and true-triaxial stress.During the research and development process,five key technologies were successfully broken through:(1)the ability to perform true-triaxial compressioneshear loading tests on rock samples with high stiffness;(2)a shear box with ultra-low friction throughout the entire stress space of the rock sample during loading;(3)a control system capable of maintaining high stress for a long time and responding rapidly to the brittle fracture of a rock sample as well;(4)a refined ability to measure the volumetric deformation of rock samples subjected to true triaxial shearing;and(5)a heating system capable of maintaining uniform heating of the rock sample over a long time.By developing these technologies,loading under high true triaxial stress conditions was realized.The apparatus has a maximum normal stiffness of 1000 GPa/m and a maximum operating temperature of 300C.The differences in the surface temperature of the sample are constant to within5C.Five types of true triaxial shear tests were conducted on homogeneous sandstone to verify that the apparatus has good performance and reliability.The results show that temperature,lateral stress,normal stress and time influence the shear deformation,failure mode and strength of the sandstone.The novel apparatus can be reliably used to conduct true-triaxial shear tests on rocks subjected to high temperatures and stress. 展开更多
关键词 True-triaxial shear apparatus ROCKS Complete shear stress-deformation process CREEP real-time high-temperature
在线阅读 下载PDF
Review of Some Advances and Applications in Real-time High-speed Vision: Our Views and Experiences 被引量:2
8
作者 Qing-Yi Gu Idaku Ishii 《International Journal of Automation and computing》 EI CSCD 2016年第4期305-318,共14页
The frame rate of conventional vision systems is restricted to the video signal formats (e.g., NTSC 30 fps and PAL 25 fps) that are designed on the basis of the characteristics of the human eye, which implies that t... The frame rate of conventional vision systems is restricted to the video signal formats (e.g., NTSC 30 fps and PAL 25 fps) that are designed on the basis of the characteristics of the human eye, which implies that the processing speed of these systems is limited to the recognition speed of the human eye. However, there is a strong demand for real-time high-speed vision sensors in many application fields, such as factory automation, biomedicine, and robotics, where high-speed operations are carried out. These high-speed operations can be tracked and inspected by using high-speed vision systems with intelligent sensors that work at hundreds of Hertz or more, especially when the operation is difficult to observe with the human eye. This paper reviews advances in developing real-time high Speed vision systems and their applications in various fields, such as intelligent logging systems, vibration dynamics sensing, vision-based mechanical control, three-dimensional measurement/automated visual inspection, vision-based human interface, and biomedical applications. 展开更多
关键词 real-time high-speed vision target tracking abnormal behavior detection behavior mining vibration analysis 3D shapemeasurement cell sorting.
原文传递
A machine vision approach to seam tracking in real-time in PAW of large-diameter stainless steel tube 被引量:1
9
作者 葛景国 朱政强 +1 位作者 何德孚 陈立功 《China Welding》 EI CAS 2004年第2期151-155,共5页
Manual monitoring and seam tracking through watching weld pool images in real-time, by naked eyes or by industrial TV, are experience-depended, subjective, labor intensive, and sometimes biased. So it is necessary to ... Manual monitoring and seam tracking through watching weld pool images in real-time, by naked eyes or by industrial TV, are experience-depended, subjective, labor intensive, and sometimes biased. So it is necessary to realize the automation of computer-aided seam tracking. A PAW (plasma arc welding) seam tracking system was developed, which senses the molten pool and the seam in one frame by a vision sensor, and then detects the seam deviation to adjust the work piece motion adaptively to the seam position sensed by vision sensor. A novel molten pool area image-processing algorithm based on machine vision was proposed. The algorithm processes each image at the speed of 20 frames/second in real-time to extract three feature variables to get the seam deviation. It is proved experimentally that the algorithm is very fast and effective. Issues related to the algorithm are also discussed. 展开更多
关键词 ALGORITHM seam tracking image processing real-time machine vision plasma arc welding
在线阅读 下载PDF
Efficiency analysis of numerical integrations for finite element substructure in real-time hybrid simulation 被引量:5
10
作者 Wang Jinting Lu Liqiao Zhu Fei 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2018年第1期73-86,共14页
Finite element(FE) is a powerful tool and has been applied by investigators to real-time hybrid simulations(RTHSs). This study focuses on the computational efficiency, including the computational time and accuracy... Finite element(FE) is a powerful tool and has been applied by investigators to real-time hybrid simulations(RTHSs). This study focuses on the computational efficiency, including the computational time and accuracy, of numerical integrations in solving FE numerical substructure in RTHSs. First, sparse matrix storage schemes are adopted to decrease the computational time of FE numerical substructure. In this way, the task execution time(TET) decreases such that the scale of the numerical substructure model increases. Subsequently, several commonly used explicit numerical integration algorithms, including the central difference method(CDM), the Newmark explicit method, the Chang method and the Gui-λ method, are comprehensively compared to evaluate their computational time in solving FE numerical substructure. CDM is better than the other explicit integration algorithms when the damping matrix is diagonal, while the Gui-λ(λ = 4) method is advantageous when the damping matrix is non-diagonal. Finally, the effect of time delay on the computational accuracy of RTHSs is investigated by simulating structure-foundation systems. Simulation results show that the influences of time delay on the displacement response become obvious with the mass ratio increasing, and delay compensation methods may reduce the relative error of the displacement peak value to less than 5% even under the large time-step and large time delay. 展开更多
关键词 real-time hybrid simulation computational efficiency numerical integration storage optimization time delay
在线阅读 下载PDF
Portable FBAR based E-nose for cold chain real-time bananas shelf time detection 被引量:1
11
作者 Chen Wu Jiuyan Li 《Nanotechnology and Precision Engineering》 CAS CSCD 2023年第1期32-39,共8页
Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and... Being cheap,nondestructive,and easy to use,gas sensors play important roles in the food industry.However,most gas sensors are suitable more for laboratory-quality fast testing rather than for cold-chain continuous and cumulative testing.Also,an ideal electronic nose(E-nose)in a cold chain should be stable to its surroundings and remain highly accurate and portable.In this work,a portable film bulk acoustic resonator(FBAR)-based E-nose was built for real-time measurement of banana shelf time.The sensor chamber to contain the portable circuit of the E-nose is as small as a smartphone,and by introducing an air-tight FBAR as a reference,the E-nose can avoid most of the drift caused by surroundings.With the help of porous layer by layer(LBL)coating of the FBAR,the sensitivity of the E-nose is 5 ppm to ethylene and 0.5 ppm to isoamyl acetate and isoamyl butyrate,while the detection range is large enough to cover a relative humidity of 0.8.In this regard,the E-nose can easily discriminate between yellow bananas with green necks and entirely yellow bananas while allowing the bananas to maintain their biological activities in their normal storage state,thereby showing the possibility of real-time shelf time detection.This portable FBAR-based E-nose has a large testing scale,high sensitivity,good humidity tolerance,and low frequency drift to its surroundings,thereby meeting the needs of cold-chain usage. 展开更多
关键词 Film bulk acoustic resonator(FBAR) Portable E-nose real-time detection Layer by layer
在线阅读 下载PDF
RFID Complex Event Processing: Applications in Real-Time Locating System 被引量:2
12
作者 Yao-zong Liu Hong Zhang Yong-li Wang 《International Journal of Intelligence Science》 2012年第4期160-165,共6页
Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP,... Complex event processing (CEP) can extract meaningful events for real-time locating system (RTLS) applications. To identify complex event accurately in RTLS, we propose a new RFID complex event processing method GEEP, which is based on the timed automata (TA) theory. By devising RFID locating application into complex events, we model the timing diagram of RFID data streams based on the TA. We optimize the constraint of the event streams and propose a novel method to derive the constraint between objects, as well as the constraint between object and location. Experiments prove the proposed method reduces the cost of RFID complex event processing, and improves the efficiency of the RTLS. 展开更多
关键词 Complex Event Processing (CEP) real-time Locating System (RTLS) Radio Frequency Identification (RFID) TIMED AUTOMATA (TA) Event-Clock AUTOMATA (ECA)
在线阅读 下载PDF
Weak Serializable Concurrency Control in Real-time Broadcast Environments
13
作者 刘云生 Dang Depeng 《High Technology Letters》 EI CAS 2002年第4期64-68,共5页
Given the asymmetric communication capabilities in mobile real-time environments, traditional serializability-based approaches are too restrictive, unnecessary, and impractical. The new notion of correctness called we... Given the asymmetric communication capabilities in mobile real-time environments, traditional serializability-based approaches are too restrictive, unnecessary, and impractical. The new notion of correctness called weak serializability is given. After the necessary and sufficient conditions for weak serializability are shown, corresponding concurrency control protocol based on this criterion is outlined for real-time broadcast environments. The simulation results confirmed that the proposed techniques help mobile transactions to meet their deadlines and improve query response time. 展开更多
关键词 real-time data broadcast real-time concurrency control relaxing serializability
在线阅读 下载PDF
Alternative Approach for Studying Contractility of the Isolated Muscle Preparations in Real-Time Mode
14
作者 Alexander Balakin Ruslan Lisin +1 位作者 Alexey Smoluk Yuri Protsenko 《Journal of Biomedical Science and Engineering》 2015年第9期643-652,共10页
The real-time control over the observed parameters is known to be necessary during the experiments with biological objects. The use of approaches, such as real-time operating systems, often requires significant materi... The real-time control over the observed parameters is known to be necessary during the experiments with biological objects. The use of approaches, such as real-time operating systems, often requires significant material or human resources (operating time, the special administration and programming skills and etc.). In this paper we propose an alternative approach for the implementation of real-time mode in the experiments on muscle preparations (papillary muscles, trabeculae, walls of vessels) based on an external input-output cards with built-in analog-to-digital/digital-to-analog converters and signal processor. As a result, we developed a hardware-software complex for studying the mechanical properties of biological materials (muscles) in real-time mode. This solution has a convenient and easy-to-use user interface, and requires a minimum of computing resources and minimal cost of electricity. 展开更多
关键词 Software MUSCLE TISSUE real-time
暂未订购
Cornwall-Jackiw-Tomboulis Effective Potential for Quark Propagator in Real-Time Thermal Field Theory and Landau Gauge
15
作者 ZHOU Bang-Rong 《Communications in Theoretical Physics》 SCIE CAS CSCD 2005年第5X期879-882,共4页
We complete the derivation of the Cornwall-Jackiw-Tomboulis effective potentiM for quark propagator at finite temperature and finite quark chemical potential in the real-time formalism of thermal field theory and in L... We complete the derivation of the Cornwall-Jackiw-Tomboulis effective potentiM for quark propagator at finite temperature and finite quark chemical potential in the real-time formalism of thermal field theory and in Landau gauge. In the approximation that the function A(p^2) in inverse quark propagator is replaced by unity, by means of the running gauge coupling and the quark mass function invariant under the renormalization group in zero temperature Quantum Chromadynamics (QCD), we obtain a calculable expression for the thermal effective potential, which will be a useful means to research chiral phase transition in QCD in the real-time formalism. 展开更多
关键词 CJT effective potential real-time thermal field theory QCD quark propagator Landau gauge
在线阅读 下载PDF
Shadow Volume in Real-Time Rendering
16
作者 Abd El Moumene Zerari Mohamed Chaouki Babahenini 《Computer Technology and Application》 2011年第8期638-643,共6页
This paper presents an optimization of shadow volume algorithm, which allow a rendering in real-time. This technique is based on previous works which makes it possible to obtain shadows in real-time, although the calc... This paper presents an optimization of shadow volume algorithm, which allow a rendering in real-time. This technique is based on previous works which makes it possible to obtain shadows in real-time, although the calculation of the silhouette requires a pretreatment of the geometry implemented on the CPU (Central Processing Unit). By using last version of the GPU (Graphic Processing Unit), the authors propose to implement the calculation of the silhouette on the GPU by using Geometry Shader. The authors present the step which made it possible to lead to a concrete implementation of this algorithm, the modifications which were made, as well as a comparative study of results, followed by a discussion of these results and choices of implementation. 展开更多
关键词 Shadow volumes SILHOUETTE GPU (graphic processing unit) real-time shaders.
在线阅读 下载PDF
Analyzing the Impact of Scene Transitions on Indoor Camera Localization through Scene Change Detection in Real-Time
17
作者 Muhammad S.Alam Farhan B.Mohamed +2 位作者 Ali Selamat Faruk Ahmed AKM B.Hossain 《Intelligent Automation & Soft Computing》 2024年第3期417-436,共20页
Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance o... Real-time indoor camera localization is a significant problem in indoor robot navigation and surveillance systems.The scene can change during the image sequence and plays a vital role in the localization performance of robotic applications in terms of accuracy and speed.This research proposed a real-time indoor camera localization system based on a recurrent neural network that detects scene change during the image sequence.An annotated image dataset trains the proposed system and predicts the camera pose in real-time.The system mainly improved the localization performance of indoor cameras by more accurately predicting the camera pose.It also recognizes the scene changes during the sequence and evaluates the effects of these changes.This system achieved high accuracy and real-time performance.The scene change detection process was performed using visual rhythm and the proposed recurrent deep architecture,which performed camera pose prediction and scene change impact evaluation.Overall,this study proposed a novel real-time localization system for indoor cameras that detects scene changes and shows how they affect localization performance. 展开更多
关键词 Camera pose estimation indoor camera localization real-time localization scene change detection simultaneous localization and mapping(SLAM)
在线阅读 下载PDF
Combining machine learning and physics modeling to determine the geostress of reservoirs in real-time
18
作者 Quansheng Liu Fei Gao +1 位作者 Mengmeng Nie Jiangmei Qiao 《Intelligent Geoengineering》 2024年第1期69-77,共9页
Real-time updating of geological properties in reservoirs through monitoring data is critical for optimizing hydraulic fracturing.In this work,the ML-Physics method,which inherits the advantages of machine learning(ML... Real-time updating of geological properties in reservoirs through monitoring data is critical for optimizing hydraulic fracturing.In this work,the ML-Physics method,which inherits the advantages of machine learning(ML)and physical modeling,has been developed to determine the geological parameters with limited computational time,including farfield geostress,geostress in the vicinity of faults and rock material properties.The ML-Physics method consists of two stages:the preparation stage and the operation stage.During the preparation stage,which occurs before the in situ operation and is not constrained by computational time limitation,a surrogate artificial neural network(ANN)model is established.This ANN model captures the implicit relationship between geological parameters and monitoring data.During the operational stage,which is constrained by real-time requirements for the convenience of engineers in field work,inverse analysis with physical modeling and the surrogate ANN model is employed to determine geological parameters.In the context of ML-Physics method,the initial values of inverse iteration are chosen based on surrogate ANN results.The obtained geological parameters can be used for hydraulic fracturing analysis.This ML-Physics method demonstrates superior performance in terms of both accuracy and computational efficiency. 展开更多
关键词 ML-physics method Machine learning Inverse analysis Geostress Hydraulic fracturing real-time optimization
在线阅读 下载PDF
奶牛乳房炎病原体三重Real-time PCR检测方法的建立及应用
19
作者 郭思宇 高雅欣 +5 位作者 纪佳豪 李梓豪 刘文扬 徐博 王三毛 李睿文 《动物医学进展》 北大核心 2025年第12期39-44,共6页
为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检... 为了建立同时检测奶牛临床型乳房炎中肺炎克雷伯菌(Kp)、产色葡萄球菌(Sc)和牛支原体(Mb),基于Kp ZKIR基因、Sc sodA基因和Mb opp D/F基因设计特异性引物,建立三重实时定量荧光PCR方法(real-time PCR)。试验采用在单一real-time PCR检测方法的基础上对三重real-time PCR检测方法进行优化,并确定退火条件为60℃,肺炎克雷伯菌、产色葡萄球菌以及牛支原体上、下游引物浓度为20μmol/L、荧光探针浓度为10μmol/L。结果表明,该方法对标准品pUC57-ZKIR-Kp、pUC57-sodA-Sc、pUC57-opp D/F-Mb最低检测限分别为1.55×10^(2) copies/μL、1.44×10^(2) copies/μL、1.34×10^(2) copies/μL,灵敏度高;仅对Kp、Sc、Mb这3种病原产生荧光曲线,对其他病原无交叉反应,特异性强;其中组内、组间变异系数均小于2%,重复性良好。利用建立的多重real-time PCR对233份临床样品进行检测,Kp、Sc、Mb检出率分别为73.09%、21.97%、6.72%,与单一real-time PCR方法检测结果一致。说明建立的多重real-time PCR在实际应用中具有灵敏度高、特异性强、重复性良好、检测速度快等优点,可为奶牛临床型乳房炎病原的快速检测、临床诊断和流行病学调查提供有效检测手段。 展开更多
关键词 临床型乳房炎 三重real-time PCR 肺炎克雷伯菌 产色葡萄球菌 牛支原体
在线阅读 下载PDF
IoT-Based Real-Time Medical-Related Human Activity Recognition Using Skeletons and Multi-Stage Deep Learning for Healthcare 被引量:1
20
作者 Subrata Kumer Paul Abu Saleh Musa Miah +3 位作者 Rakhi Rani Paul Md.EkramulHamid Jungpil Shin Md Abdur Rahim 《Computers, Materials & Continua》 2025年第8期2513-2530,共18页
The Internet of Things(IoT)and mobile technology have significantly transformed healthcare by enabling real-time monitoring and diagnosis of patients.Recognizing Medical-Related Human Activities(MRHA)is pivotal for he... The Internet of Things(IoT)and mobile technology have significantly transformed healthcare by enabling real-time monitoring and diagnosis of patients.Recognizing Medical-Related Human Activities(MRHA)is pivotal for healthcare systems,particularly for identifying actions critical to patient well-being.However,challenges such as high computational demands,low accuracy,and limited adaptability persist in Human Motion Recognition(HMR).While some studies have integrated HMR with IoT for real-time healthcare applications,limited research has focused on recognizing MRHA as essential for effective patient monitoring.This study proposes a novel HMR method tailored for MRHA detection,leveraging multi-stage deep learning techniques integrated with IoT.The approach employs EfficientNet to extract optimized spatial features from skeleton frame sequences using seven Mobile Inverted Bottleneck Convolutions(MBConv)blocks,followed by Convolutional Long Short Term Memory(ConvLSTM)to capture spatio-temporal patterns.A classification module with global average pooling,a fully connected layer,and a dropout layer generates the final predictions.The model is evaluated on the NTU RGB+D 120 and HMDB51 datasets,focusing on MRHA such as sneezing,falling,walking,sitting,etc.It achieves 94.85%accuracy for cross-subject evaluations and 96.45%for cross-view evaluations on NTU RGB+D 120,along with 89.22%accuracy on HMDB51.Additionally,the system integrates IoT capabilities using a Raspberry Pi and GSM module,delivering real-time alerts via Twilios SMS service to caregivers and patients.This scalable and efficient solution bridges the gap between HMR and IoT,advancing patient monitoring,improving healthcare outcomes,and reducing costs. 展开更多
关键词 real-time human motion recognition(HMR) ENConvLSTM EfficientNet ConvLSTM skeleton data NTU RGB+D 120 dataset MRHA
在线阅读 下载PDF
上一页 1 2 122 下一页 到第
使用帮助 返回顶部