Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology,because abnormal cellular morphology is an i...Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology,because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states.In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe.Imaging flow cytometry of large populations of S.pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells.Several natural products were found to alter S.pombe’s morphology relative to control,in terms of elongating cells,shrinking them,or making them more round.These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.展开更多
Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such a...Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such as biomedicine,green energy,and environmental monitoring.However,due to limitations in imaging framerate and realtime data processing,the real-time throughput of existing IFC systems has been restricted to approximately 1000-10,000 events per second(eps),which is insufficient for large-scale cell analysis.In this work,we demonstrate IFC with real-time throughput exceeding 1,000,000 eps by integrating optical time-stretch(OTS)imaging,microfluidic-based cell manipulation,and online image processing.Cells flowing at speeds up to 15 m/s are clearly imaged with a spatial resolution of 780 nm,and images of each individual cell are captured,stored,and analyzed.The capabilities and performance of our system are validated through the identification of malignancies in clinical colorectal samples.This work sets a new record for throughput in imaging flow cytometry,and we believe it has the potential to revolutionize cell analysis by enabling highly efficient,accurate,and intelligent measurement.展开更多
As the gold-standard method for single-cell analysis,flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells.This review highlights the demands for clinical flow c...As the gold-standard method for single-cell analysis,flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells.This review highlights the demands for clinical flow cytometry in laboratory hematology(e.g.,diagnoses of minimal residual disease and various types of leukemia),summarizes state-of-the-art clinical flow cytometers(e.g.,FACSLyricTMby Becton Dickinson,DxFLEX by Beckman Coulter),then considers innovative technical improvements in flow cytometry(including quantitative,spectral,and imaging approaches)to address the limitations of clinical flow cytometry in hematology diagnosis.Finally,driven by these clinical demands,future developments in clinical flow cytometry are suggested.展开更多
Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, ...Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, such as magnetic resonance imaging （MRI）, aerospace remote sensing, terahertz photography, and hyperspectral imaging. Compared with conventional silicon-based cameras, single-pixel cameras （SPCs） can achieve image compression and operate over a much broader spectral range. However, the imaging speed of SPCs is governed by the response time of digital mieromirror devices （DMDs） and the amount of com- pression of acquired images, leading to low （ms-level） temporal resolution. Consequently, it is particularly challenging for SPCs to investigate fast dynamic phenomena, which is required commonly in microscopy. Recently, a unique approach based on photonic time stretch （PTS） to achieve high-speed SPI has been reported. It achieves a frame rate far beyond that can be reached with conventional SPCs. In this paper, we first introduce the principles and applications of the PTS technique. Then the basic archi- tecture of the high-speed SPI system is presented, and an imaging flow cytometer with high speed and high throughput is demonstrated experimentally. Finally, the limitations and potential applications of high-speed SPI are discussed.展开更多
基金financial support from the University of North Florida.
文摘Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology,because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states.In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe.Imaging flow cytometry of large populations of S.pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells.Several natural products were found to alter S.pombe’s morphology relative to control,in terms of elongating cells,shrinking them,or making them more round.These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.
基金supported by the National Key R&D Program of China(2023YFF0723300)National Natural Science Foundation of China(62475198,62075200,12374295)+8 种基金Fundamental Research Funds for the Central Universities(2042024kf0003,2042024kf1010,2042023kf0105)Hubei Provincial Natural Science Foundation of China(2023AFB133)Jiangsu Science and Technology Program(BK20221257)Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014)Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217,ZNJC202232)The Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006)Hubei Province Young Science and Technology Talent Morning Hight Lift Project(202319)The Fund of National Key Laboratory of Plasma Physics(6142A04230201)We gratefully acknowledge Serendipity Lab for facilitating collaboration opportunities.
文摘Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such as biomedicine,green energy,and environmental monitoring.However,due to limitations in imaging framerate and realtime data processing,the real-time throughput of existing IFC systems has been restricted to approximately 1000-10,000 events per second(eps),which is insufficient for large-scale cell analysis.In this work,we demonstrate IFC with real-time throughput exceeding 1,000,000 eps by integrating optical time-stretch(OTS)imaging,microfluidic-based cell manipulation,and online image processing.Cells flowing at speeds up to 15 m/s are clearly imaged with a spatial resolution of 780 nm,and images of each individual cell are captured,stored,and analyzed.The capabilities and performance of our system are validated through the identification of malignancies in clinical colorectal samples.This work sets a new record for throughput in imaging flow cytometry,and we believe it has the potential to revolutionize cell analysis by enabling highly efficient,accurate,and intelligent measurement.
基金financial support of the National Natural Science Foundation of China(Grant Nos.61922079,61825107,and 62121003)the Chinese Academy of Sciences(Grant Nos.GJJSTD20210004 and Y201927)the National Key Research and Development Program of China(Grant No.2021YFC2500300).
文摘As the gold-standard method for single-cell analysis,flow cytometry enables high-throughput and multiple-parameter characterization of individual biological cells.This review highlights the demands for clinical flow cytometry in laboratory hematology(e.g.,diagnoses of minimal residual disease and various types of leukemia),summarizes state-of-the-art clinical flow cytometers(e.g.,FACSLyricTMby Becton Dickinson,DxFLEX by Beckman Coulter),then considers innovative technical improvements in flow cytometry(including quantitative,spectral,and imaging approaches)to address the limitations of clinical flow cytometry in hematology diagnosis.Finally,driven by these clinical demands,future developments in clinical flow cytometry are suggested.
基金Project supported by the National Natural Science Foundation of China （Nos. 61771284 and 61322113）
文摘Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, such as magnetic resonance imaging （MRI）, aerospace remote sensing, terahertz photography, and hyperspectral imaging. Compared with conventional silicon-based cameras, single-pixel cameras （SPCs） can achieve image compression and operate over a much broader spectral range. However, the imaging speed of SPCs is governed by the response time of digital mieromirror devices （DMDs） and the amount of com- pression of acquired images, leading to low （ms-level） temporal resolution. Consequently, it is particularly challenging for SPCs to investigate fast dynamic phenomena, which is required commonly in microscopy. Recently, a unique approach based on photonic time stretch （PTS） to achieve high-speed SPI has been reported. It achieves a frame rate far beyond that can be reached with conventional SPCs. In this paper, we first introduce the principles and applications of the PTS technique. Then the basic archi- tecture of the high-speed SPI system is presented, and an imaging flow cytometer with high speed and high throughput is demonstrated experimentally. Finally, the limitations and potential applications of high-speed SPI are discussed.
