采用开顶式气室(open top chambers,OTCs)装置,研究O3浓度升高(E-O3,约150μL.L-1)对常绿阔叶树种全缘冬青幼苗的影响,主要包括伤害症状、植株生长、光合色素含量、气体交换速率与叶绿素荧光、丙二醛含量以及主要抗氧化剂含量变化.经过...采用开顶式气室(open top chambers,OTCs)装置,研究O3浓度升高(E-O3,约150μL.L-1)对常绿阔叶树种全缘冬青幼苗的影响,主要包括伤害症状、植株生长、光合色素含量、气体交换速率与叶绿素荧光、丙二醛含量以及主要抗氧化剂含量变化.经过高浓度O3处理一个生长季后,当年生全展叶片表面分布着大量的黑褐色斑点与斑块,呈腐蚀状凹陷于叶片表面.O3引起茎百分比显著降低7%,但对株高与基径生长、生物量累积与比叶重无显著性影响;与CF处理相比,O3处理下全缘冬青幼苗当年生全展叶片净光合速率、叶绿素a/b值与总酚含量分别显著降低19%、9%与36%.气孔导度、胞间CO2浓度、叶绿素荧光参数、光合色素含量、叶片丙二醛含量、总还原能力以及抗坏血酸含量的变化均不显著.结果表明,O3对全缘冬青幼苗的光合作用具有一定的抑制作用,叶绿素含量组成发生变化是引起净光合速率下降的主要原因;O3对植株总的抗氧化能力未产生明显影响,尚未引起膜脂过氧化程度加剧;虽然全缘冬青幼苗叶片呈现伤害症状,但植株生长指标未受到O3的显著影响.展开更多
Determining the responses of candidate plants to salt stress is a prerequisite for selecting and breeding suitable plants with high salt tolerance to grow in coastal mudfl at areas with high salinity.Here,2-year cutti...Determining the responses of candidate plants to salt stress is a prerequisite for selecting and breeding suitable plants with high salt tolerance to grow in coastal mudfl at areas with high salinity.Here,2-year cutting seedlings of Ilex purpurea Hassk.(local species)and I.integra Thunb.(introduced species)were grown in pots in a glasshouse and irrigated with a Hoagland-NaCl solution at 0,24,and 48 h.Root samples were collected at 0,1,6,24,and 72 h,and concentration of Na^(+)ion;content of proline,soluble carbohydrate,malondialdehyde(MDA),H_(2)O_(2) and ascorbate;and activity of three key antioxidative enzymes were measured.Roots of I.integra accumulated relatively less Na^(+)and had less membrane lipid peroxidation and H_(2)O_(2) during salt stress,thus indicating a relatively higher salt tolerance than roots of I.purpurea.Values for ascorbate content and antioxidant enzymatic activity suggest that the antioxidant ascorbate and antioxidative catalase may play substantial roles for scavenging reactive oxygen species in I.integra roots during salt treatment.Thus,I.integra is apparently more suitable for growing in local highly saline coastal mudfl ats.展开更多
文摘采用开顶式气室(open top chambers,OTCs)装置,研究O3浓度升高(E-O3,约150μL.L-1)对常绿阔叶树种全缘冬青幼苗的影响,主要包括伤害症状、植株生长、光合色素含量、气体交换速率与叶绿素荧光、丙二醛含量以及主要抗氧化剂含量变化.经过高浓度O3处理一个生长季后,当年生全展叶片表面分布着大量的黑褐色斑点与斑块,呈腐蚀状凹陷于叶片表面.O3引起茎百分比显著降低7%,但对株高与基径生长、生物量累积与比叶重无显著性影响;与CF处理相比,O3处理下全缘冬青幼苗当年生全展叶片净光合速率、叶绿素a/b值与总酚含量分别显著降低19%、9%与36%.气孔导度、胞间CO2浓度、叶绿素荧光参数、光合色素含量、叶片丙二醛含量、总还原能力以及抗坏血酸含量的变化均不显著.结果表明,O3对全缘冬青幼苗的光合作用具有一定的抑制作用,叶绿素含量组成发生变化是引起净光合速率下降的主要原因;O3对植株总的抗氧化能力未产生明显影响,尚未引起膜脂过氧化程度加剧;虽然全缘冬青幼苗叶片呈现伤害症状,但植株生长指标未受到O3的显著影响.
基金supported by the Key Research and Development Program of Jiangsu Province(Modern agriculture)(BE2018400)by Innovation Capacity Building Plan(Science and Technology Facilities)-Independent Research Fund for Research Institutes of Public Welfare of Jiangsu Province(BM2018022-6)。
文摘Determining the responses of candidate plants to salt stress is a prerequisite for selecting and breeding suitable plants with high salt tolerance to grow in coastal mudfl at areas with high salinity.Here,2-year cutting seedlings of Ilex purpurea Hassk.(local species)and I.integra Thunb.(introduced species)were grown in pots in a glasshouse and irrigated with a Hoagland-NaCl solution at 0,24,and 48 h.Root samples were collected at 0,1,6,24,and 72 h,and concentration of Na^(+)ion;content of proline,soluble carbohydrate,malondialdehyde(MDA),H_(2)O_(2) and ascorbate;and activity of three key antioxidative enzymes were measured.Roots of I.integra accumulated relatively less Na^(+)and had less membrane lipid peroxidation and H_(2)O_(2) during salt stress,thus indicating a relatively higher salt tolerance than roots of I.purpurea.Values for ascorbate content and antioxidant enzymatic activity suggest that the antioxidant ascorbate and antioxidative catalase may play substantial roles for scavenging reactive oxygen species in I.integra roots during salt treatment.Thus,I.integra is apparently more suitable for growing in local highly saline coastal mudfl ats.
