勃起功能障碍(ED)是男性常见的多因素疾病,其发病机制涉及血管内皮功能障碍,氧化应激,炎症反应和神经内分泌等疾病。丹参酮IIA (Tanshinone IIA)是从丹参中提取的主要活性成分,具有抗氧化、抗炎、改善微循环和保护血管内皮等药理作用。...勃起功能障碍(ED)是男性常见的多因素疾病,其发病机制涉及血管内皮功能障碍,氧化应激,炎症反应和神经内分泌等疾病。丹参酮IIA (Tanshinone IIA)是从丹参中提取的主要活性成分,具有抗氧化、抗炎、改善微循环和保护血管内皮等药理作用。近年来,研究发现丹参酮IIA可以通过调节一氧化氮(NO)通路,抑制氧化应激和炎症反应来改善ED症状,但其具体机制仍有待进一步探讨。本文就丹参酮IIA的化学特性、药理作用;丹参酮IIA与勃起功能障碍的关联;丹参酮IIA治疗ED的研究进展进行如下综述,为ED的靶向治疗提供新思路。Erectile dysfunction (ED) is a common multifactorial disease in men, and its pathogenesis involves vascular endothelial dysfunction, oxidative stress, inflammatory response, and neuroendocrine disorders. Tanshinone IIA is the main active ingredient extracted from Salvia miltiorrhizae, which has pharmacological effects such as antioxidant, anti-inflammatory, improving microcirculation and protecting vascular endothelium. In recent years, research has shown that Tanshinone IIA can improve erectile dysfunction (ED) symptoms by modulating the nitric oxide (NO) pathway and inhibiting oxidative stress and inflammatory responses. However, the specific mechanisms still need to be further explored. This article reviews the chemical properties and pharmacological effects of Tanshinone IIA, the association between Tanshinone IIA and erectile dysfunction, and the research progress of Tanshinone IIA in treating ED, providing new ideas for targeted therapy of ED.展开更多
丹参酮IIA (Tanshinone IIA, TanIIA)是从中药丹参中提取的主要有效成分之一,研究表明其在抗炎、抗氧化、抗肿瘤、心脏保护及神经系统保护方面具有显著的药理活性。本文采用文献计量学的方法,对国内外有关丹参酮IIA的研究进行分析,发现...丹参酮IIA (Tanshinone IIA, TanIIA)是从中药丹参中提取的主要有效成分之一,研究表明其在抗炎、抗氧化、抗肿瘤、心脏保护及神经系统保护方面具有显著的药理活性。本文采用文献计量学的方法,对国内外有关丹参酮IIA的研究进行分析,发现丹参酮IIA的研究是基于2000年至2024年间发表的13,681篇有关丹参酮IIA的文献的计量学分析;该领域发表研究论文最多的国家是中国,美国和澳大利亚,而发表论文最多的机构为中国医科大学,中国科学院和中山大学。丹参酮IIA在心血管疾病和神经系统疾病中的研究文献占比最高,研究类型主要以应用基础研究,临床研究和技术开发为主,出现频率最多的关键词为凋亡,表达,激活,氧化应激,炎症反应等,体现了其在这些领域的研究热度和临床应用价值。丹参酮IIA作为一种具有多重药理活性的中药成分,显示出广泛的临床应用前景,而当前研究的局限性和挑战需要我们在未来的研究中予以重视,通过多学科合作和创新研究方法,共同推动丹参酮IIA在疾病治疗中的进一步应用与发展。Tanshinone IIA (TanIIA) is one of the main active ingredients extracted from the traditional Chinese medicine Danshen, and studies have shown that it has significant pharmacological activities in anti-inflammatory, antioxidant, anti-tumor, cardioprotective and nervous system protection. In this paper, bibliometric methods were used to analyze the studies on tanshinone IIA at home and abroad, and found that the research on tanshinone IIA was based on quantitative analysis of 13,681 articles on tanshinone IIA published between 2000 and 2024. The countries with the most published research papers in this field are China, United States and Australia, while the institutions with the most published papers are China Medical University, Chinese Academy of Sciences and Sun Yat-sen University. Tanshinone IIA has the highest proportion of research literature in cardiovascular diseases and nervous system diseases, and the research types are mainly applied basic research, clinical research and technology development, and the most frequent keywords are apoptosis, expression, activation, oxidative stress, inflammatory response, etc., reflecting its research enthusiasm and clinical application value in these fields. As a traditional Chinese medicine ingredient with multiple pharmacological activities, tanshinone IIA has shown a wide range of clinical application prospects, but the limitations and challenges of current research need to be paid attention to in future research, and jointly promote the further application and development of tanshinone IIA in disease treatment through multidisciplinary cooperation and innovative research methods.展开更多
丹参酮IIA,也称为TanIIA,在生物体内代谢过程中扮演着多重角色,其产物能够影响多种生物化学反应。这些反应中,TanIIA有时作为辅酶参与,有时则可能促进或干扰这些反应过程,从而展现出多样的药理特性。尽管在脊髓损伤等神经系统疾病的治疗...丹参酮IIA,也称为TanIIA,在生物体内代谢过程中扮演着多重角色,其产物能够影响多种生物化学反应。这些反应中,TanIIA有时作为辅酶参与,有时则可能促进或干扰这些反应过程,从而展现出多样的药理特性。尽管在脊髓损伤等神经系统疾病的治疗中,TanIIA已经显示出了一定的疗效,但其具体的作用机制尚未完全阐明。基于现有的研究成果,我们推测TanIIA可能通过多种药理途径,包括抗炎、抗氧化、抗细胞凋亡以及保护血管,来促进脊髓损伤后的神经细胞存活和修复。Tanshinone IIA, also known as TanIIA, plays multiple roles in the metabolic process of living organisms, and its products can affect a variety of biochemical reactions. In these reactions, TanIIA is sometimes involved as a coenzyme, and sometimes it may facilitate or interfere with these reaction processes, thus exhibiting diverse pharmacological properties. Although TanIIA has shown some efficacy in the treatment of neurological diseases such as spinal cord injury, its specific mechanism of action has not been fully elucidated. Based on the existing research results, we speculate that TanIIA may promote the survival and repair of nerve cells after spinal cord injury through multiple pharmacological pathways, including anti-inflammatory, antioxidant, anti-apoptosis, and vascular protection.展开更多
Evidence indicates that metabolic reprogramming characterized by the changes in cellular metabolic patterns contributes to the pathogenesis of pulmonary fibrosis (PF). It is considered as a promising therapeutic targe...Evidence indicates that metabolic reprogramming characterized by the changes in cellular metabolic patterns contributes to the pathogenesis of pulmonary fibrosis (PF). It is considered as a promising therapeutic target anti-PF. The well-documented against PF properties of Tanshinone IIA (Tan IIA) have been primarily attributed to its antioxidant and anti-inflammatory potency. Emerging evidence suggests that Tan IIA may target energy metabolism pathways, including glycolysis and tricarboxylic acid (TCA) cycle. However, the detailed and advanced mechanisms underlying the anti-PF activities remain obscure. In this study, we applied [U-13C]-glucose metabolic flux analysis (MFA) to examine metabolism flux disruption and modulation nodes of Tan IIA in PF. We identified that Tan IIA inhibited the glycolysis and TCA flux, thereby suppressing the production of transforming growth factor-β1 (TGF-β1)-dependent extracellular matrix and the differentiation and proliferation of myofibroblasts in vitro. We further revealed that Tan IIA inhibited the expression of key metabolic enzyme hexokinase 2 (HK2) by inhibiting phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/hypoxia-inducible factor 1α (HIF-1α) pathway activities, which decreased the accumulation of abnormal metabolites. Notably, we demonstrated that Tan IIA inhibited ATP citrate lyase (ACLY) activity, which reduced the collagen synthesis pathway caused by cytosol citrate consumption. Further, these results were validated in a mouse model of bleomycin-induced PF. This study was novel in exploring the mechanism of the occurrence and development of Tan IIA in treating PF using 13C-MFA technology. It provided a novel understanding of the mechanism of Tan IIA against PF from the perspective of metabolic reprogramming.展开更多
大骨节病(Kashin-Beck disease, KBD)是一种以关节软骨病变为主的地方性疾病。属中医“痹症”范畴,其发生发展与瘀血密切相关。中药以其价格低廉、疗效安全,属于天然性植物药,越来越受到研究者的关注,丹参酮IIA (Tanshinone IIA, Tan I...大骨节病(Kashin-Beck disease, KBD)是一种以关节软骨病变为主的地方性疾病。属中医“痹症”范畴,其发生发展与瘀血密切相关。中药以其价格低廉、疗效安全,属于天然性植物药,越来越受到研究者的关注,丹参酮IIA (Tanshinone IIA, Tan IIA)是中药丹参的主要有效成分,具有抗炎、保护软骨细胞外基质(ECM)、抗血小板聚集、抗血栓形成等作用,对多种痹症有效,目前尚未有丹参酮IIA应用于大骨节病的研究,本文探讨了丹参酮IIA可能应用于大骨节病的潜在机制,以期为大骨节病的治疗提供新的思路。Kashin-Beck disease (KBD) is a regional disease primarily characterized by articular cartilage lesions. It falls under the category of “bi syndrome” in traditional Chinese medicine, and its development is closely related to blood stasis. Chinese herbal medicine, known for its low cost and safe efficacy, is increasingly gaining the attention of researchers as a natural plant-based remedy. Tanshinone IIA (Tan IIA), the main active ingredient in the Chinese herb Danshen, possesses anti-inflammatory properties, protects the extracellular matrix (ECM) of chondrocytes, prevents platelet aggregation, and inhibits thrombus formation, among other effects. It has been effective against various types of bi syndrome. To date, there has been no research on the application of Tanshinone IIA in the treatment of Kashin-Beck disease. This article explores the potential mechanisms by which Tanshinone IIA could be applied to Kashin-Beck disease, with the aim of providing new perspectives for its treatment.展开更多
Background:Alveolar macrophage pyroptosis exacerbates inflammatory lung diseases,and tanshinone IIA is known for its anti-inflammatory properties.Thus,understanding how tanshinone IIA affects alveolar macrophage pyrop...Background:Alveolar macrophage pyroptosis exacerbates inflammatory lung diseases,and tanshinone IIA is known for its anti-inflammatory properties.Thus,understanding how tanshinone IIA affects alveolar macrophage pyroptosis is essential.Methods:NR8383 cells were exposed to lipopolysaccharide(LPS)and adenosine triphosphate(ATP).We assessed cell viability,pyroptosis,and the expression of triggering receptors expressed on myeloid cells 2(TREM2),p-β-catenin,β-catenin,and pyroptosis-related factors.We also examined the interaction between tanshinone IIA and TREM2.Results:Co-stimulation with LPS and ATP significantly reduced NR8383 cell viability,increased pyroptosis,and upregulated pyroptosis-associated factors.Treatment with tanshinone IIA mitigated these effects.Tanshinone IIA was effectively bound to the TREM2 protein.Knockdown of TREM2 reduced its expression and the p-β-catenin andβ-catenin levels,thereby reversing the protective effects of tanshinone IIA.Conversely,overexpression of TREM2 enhanced NR8383 cell viability,reduced pyroptosis,and suppressed pyroptosis-related factors following LPS and ATP co-stimulation.Furthermore,the NOD-like receptor family pyrin domain containing 3(NLRP3)activation reversed the reduced pyroptosis induced byβ-catenin overexpression.β-catenin knockdown reversed the protective effects of TREM2 overexpression,and activation of NLRP3 exacerbated pyroptosis inβ-catenin knockdown cells.Conclusion:Tanshinone IIA inhibited NLRP3 activation and alleviated alveolar macrophage pyroptosis through the TREM2/β-catenin pathway.展开更多
文摘勃起功能障碍(ED)是男性常见的多因素疾病,其发病机制涉及血管内皮功能障碍,氧化应激,炎症反应和神经内分泌等疾病。丹参酮IIA (Tanshinone IIA)是从丹参中提取的主要活性成分,具有抗氧化、抗炎、改善微循环和保护血管内皮等药理作用。近年来,研究发现丹参酮IIA可以通过调节一氧化氮(NO)通路,抑制氧化应激和炎症反应来改善ED症状,但其具体机制仍有待进一步探讨。本文就丹参酮IIA的化学特性、药理作用;丹参酮IIA与勃起功能障碍的关联;丹参酮IIA治疗ED的研究进展进行如下综述,为ED的靶向治疗提供新思路。Erectile dysfunction (ED) is a common multifactorial disease in men, and its pathogenesis involves vascular endothelial dysfunction, oxidative stress, inflammatory response, and neuroendocrine disorders. Tanshinone IIA is the main active ingredient extracted from Salvia miltiorrhizae, which has pharmacological effects such as antioxidant, anti-inflammatory, improving microcirculation and protecting vascular endothelium. In recent years, research has shown that Tanshinone IIA can improve erectile dysfunction (ED) symptoms by modulating the nitric oxide (NO) pathway and inhibiting oxidative stress and inflammatory responses. However, the specific mechanisms still need to be further explored. This article reviews the chemical properties and pharmacological effects of Tanshinone IIA, the association between Tanshinone IIA and erectile dysfunction, and the research progress of Tanshinone IIA in treating ED, providing new ideas for targeted therapy of ED.
文摘丹参酮IIA (Tanshinone IIA, TanIIA)是从中药丹参中提取的主要有效成分之一,研究表明其在抗炎、抗氧化、抗肿瘤、心脏保护及神经系统保护方面具有显著的药理活性。本文采用文献计量学的方法,对国内外有关丹参酮IIA的研究进行分析,发现丹参酮IIA的研究是基于2000年至2024年间发表的13,681篇有关丹参酮IIA的文献的计量学分析;该领域发表研究论文最多的国家是中国,美国和澳大利亚,而发表论文最多的机构为中国医科大学,中国科学院和中山大学。丹参酮IIA在心血管疾病和神经系统疾病中的研究文献占比最高,研究类型主要以应用基础研究,临床研究和技术开发为主,出现频率最多的关键词为凋亡,表达,激活,氧化应激,炎症反应等,体现了其在这些领域的研究热度和临床应用价值。丹参酮IIA作为一种具有多重药理活性的中药成分,显示出广泛的临床应用前景,而当前研究的局限性和挑战需要我们在未来的研究中予以重视,通过多学科合作和创新研究方法,共同推动丹参酮IIA在疾病治疗中的进一步应用与发展。Tanshinone IIA (TanIIA) is one of the main active ingredients extracted from the traditional Chinese medicine Danshen, and studies have shown that it has significant pharmacological activities in anti-inflammatory, antioxidant, anti-tumor, cardioprotective and nervous system protection. In this paper, bibliometric methods were used to analyze the studies on tanshinone IIA at home and abroad, and found that the research on tanshinone IIA was based on quantitative analysis of 13,681 articles on tanshinone IIA published between 2000 and 2024. The countries with the most published research papers in this field are China, United States and Australia, while the institutions with the most published papers are China Medical University, Chinese Academy of Sciences and Sun Yat-sen University. Tanshinone IIA has the highest proportion of research literature in cardiovascular diseases and nervous system diseases, and the research types are mainly applied basic research, clinical research and technology development, and the most frequent keywords are apoptosis, expression, activation, oxidative stress, inflammatory response, etc., reflecting its research enthusiasm and clinical application value in these fields. As a traditional Chinese medicine ingredient with multiple pharmacological activities, tanshinone IIA has shown a wide range of clinical application prospects, but the limitations and challenges of current research need to be paid attention to in future research, and jointly promote the further application and development of tanshinone IIA in disease treatment through multidisciplinary cooperation and innovative research methods.
文摘丹参酮IIA,也称为TanIIA,在生物体内代谢过程中扮演着多重角色,其产物能够影响多种生物化学反应。这些反应中,TanIIA有时作为辅酶参与,有时则可能促进或干扰这些反应过程,从而展现出多样的药理特性。尽管在脊髓损伤等神经系统疾病的治疗中,TanIIA已经显示出了一定的疗效,但其具体的作用机制尚未完全阐明。基于现有的研究成果,我们推测TanIIA可能通过多种药理途径,包括抗炎、抗氧化、抗细胞凋亡以及保护血管,来促进脊髓损伤后的神经细胞存活和修复。Tanshinone IIA, also known as TanIIA, plays multiple roles in the metabolic process of living organisms, and its products can affect a variety of biochemical reactions. In these reactions, TanIIA is sometimes involved as a coenzyme, and sometimes it may facilitate or interfere with these reaction processes, thus exhibiting diverse pharmacological properties. Although TanIIA has shown some efficacy in the treatment of neurological diseases such as spinal cord injury, its specific mechanism of action has not been fully elucidated. Based on the existing research results, we speculate that TanIIA may promote the survival and repair of nerve cells after spinal cord injury through multiple pharmacological pathways, including anti-inflammatory, antioxidant, anti-apoptosis, and vascular protection.
基金supported by the National Natural Science Foundation of China(Grant No.:82174100).
文摘Evidence indicates that metabolic reprogramming characterized by the changes in cellular metabolic patterns contributes to the pathogenesis of pulmonary fibrosis (PF). It is considered as a promising therapeutic target anti-PF. The well-documented against PF properties of Tanshinone IIA (Tan IIA) have been primarily attributed to its antioxidant and anti-inflammatory potency. Emerging evidence suggests that Tan IIA may target energy metabolism pathways, including glycolysis and tricarboxylic acid (TCA) cycle. However, the detailed and advanced mechanisms underlying the anti-PF activities remain obscure. In this study, we applied [U-13C]-glucose metabolic flux analysis (MFA) to examine metabolism flux disruption and modulation nodes of Tan IIA in PF. We identified that Tan IIA inhibited the glycolysis and TCA flux, thereby suppressing the production of transforming growth factor-β1 (TGF-β1)-dependent extracellular matrix and the differentiation and proliferation of myofibroblasts in vitro. We further revealed that Tan IIA inhibited the expression of key metabolic enzyme hexokinase 2 (HK2) by inhibiting phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/hypoxia-inducible factor 1α (HIF-1α) pathway activities, which decreased the accumulation of abnormal metabolites. Notably, we demonstrated that Tan IIA inhibited ATP citrate lyase (ACLY) activity, which reduced the collagen synthesis pathway caused by cytosol citrate consumption. Further, these results were validated in a mouse model of bleomycin-induced PF. This study was novel in exploring the mechanism of the occurrence and development of Tan IIA in treating PF using 13C-MFA technology. It provided a novel understanding of the mechanism of Tan IIA against PF from the perspective of metabolic reprogramming.
文摘大骨节病(Kashin-Beck disease, KBD)是一种以关节软骨病变为主的地方性疾病。属中医“痹症”范畴,其发生发展与瘀血密切相关。中药以其价格低廉、疗效安全,属于天然性植物药,越来越受到研究者的关注,丹参酮IIA (Tanshinone IIA, Tan IIA)是中药丹参的主要有效成分,具有抗炎、保护软骨细胞外基质(ECM)、抗血小板聚集、抗血栓形成等作用,对多种痹症有效,目前尚未有丹参酮IIA应用于大骨节病的研究,本文探讨了丹参酮IIA可能应用于大骨节病的潜在机制,以期为大骨节病的治疗提供新的思路。Kashin-Beck disease (KBD) is a regional disease primarily characterized by articular cartilage lesions. It falls under the category of “bi syndrome” in traditional Chinese medicine, and its development is closely related to blood stasis. Chinese herbal medicine, known for its low cost and safe efficacy, is increasingly gaining the attention of researchers as a natural plant-based remedy. Tanshinone IIA (Tan IIA), the main active ingredient in the Chinese herb Danshen, possesses anti-inflammatory properties, protects the extracellular matrix (ECM) of chondrocytes, prevents platelet aggregation, and inhibits thrombus formation, among other effects. It has been effective against various types of bi syndrome. To date, there has been no research on the application of Tanshinone IIA in the treatment of Kashin-Beck disease. This article explores the potential mechanisms by which Tanshinone IIA could be applied to Kashin-Beck disease, with the aim of providing new perspectives for its treatment.
基金National Natural Science Foundation of China Youth Fund(Nos.82305214 and 82004306)Excellent Youth Project of Hunan Provindial Department of Eduation(No.22B0394)+2 种基金Halth Research Project of Hunan Provincial Health Commission(No.W20243178)Hunan Provincial Adninistration of Traditional Chinese Medicine General Guidance Project(No.E2022018)Hunan Natural Science Foundation Project(No.2023JJ40401).
文摘Background:Alveolar macrophage pyroptosis exacerbates inflammatory lung diseases,and tanshinone IIA is known for its anti-inflammatory properties.Thus,understanding how tanshinone IIA affects alveolar macrophage pyroptosis is essential.Methods:NR8383 cells were exposed to lipopolysaccharide(LPS)and adenosine triphosphate(ATP).We assessed cell viability,pyroptosis,and the expression of triggering receptors expressed on myeloid cells 2(TREM2),p-β-catenin,β-catenin,and pyroptosis-related factors.We also examined the interaction between tanshinone IIA and TREM2.Results:Co-stimulation with LPS and ATP significantly reduced NR8383 cell viability,increased pyroptosis,and upregulated pyroptosis-associated factors.Treatment with tanshinone IIA mitigated these effects.Tanshinone IIA was effectively bound to the TREM2 protein.Knockdown of TREM2 reduced its expression and the p-β-catenin andβ-catenin levels,thereby reversing the protective effects of tanshinone IIA.Conversely,overexpression of TREM2 enhanced NR8383 cell viability,reduced pyroptosis,and suppressed pyroptosis-related factors following LPS and ATP co-stimulation.Furthermore,the NOD-like receptor family pyrin domain containing 3(NLRP3)activation reversed the reduced pyroptosis induced byβ-catenin overexpression.β-catenin knockdown reversed the protective effects of TREM2 overexpression,and activation of NLRP3 exacerbated pyroptosis inβ-catenin knockdown cells.Conclusion:Tanshinone IIA inhibited NLRP3 activation and alleviated alveolar macrophage pyroptosis through the TREM2/β-catenin pathway.
