The wild cotton cultivars and species have abundant genetic polymorphisms,and they possess lots of excellent genes,such as drought resistance,insect resistance,fine and strong fiber,and so on.
Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RA...Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAPD) marker tightly linked to the blast resistance gene Pi-ll(t) derived from Hongjiaozhan, which confers the resistance to race ZB1 of Pyricularia oryzae Cav.展开更多
[Objectives] To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum . [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical ...[Objectives] To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum . [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical identification was conducted on plant roots, stems, leaves, flowers and fruits. The P. capitatum powder was processed for physical and chemical distinction by FeCl 3 chromogenic reaction, hydrochloric acid magnesium powder reaction, AlCl 3 color development reaction and thin-layer chromatography.Microscope identification was carried out on the powder. Plant genome DNeasy Plant Kit was adopted for DNA molecular marker identification. [Results] The results showed that the stem of P. capitatum was tufted, the leaves were oval, 2 to 5 cm long, and 1 to 2 cm wide;the leaf apex was acute and cuneate at the base, the inflorescence was capitate, paired or solitary;the raceme was erect and nearly spherical, and the perianth was light red. Furthermore, for the chromogenic reaction of FeCl 3 ethanol extract of P. capitatum , appeared blue and turned to dark blue after long time storing at room temperature. For the reaction of hydrochloric acid magnesium powder, the alcohol extract of P. capitatum , exhibited deep red. In the color reaction of AlCl 3, the alcohol extract revealed yellow fluorescence under 360 nm UV lamp. Microscope identification of the powder displayed pollen grains, crystal sheath fibers, cellulose, vessels, starch grains, cork cells, and other characteristic fragments. In addition, DNA barcoding electrophoresis results showed that P. capitatum showed a clear and bright single band near 500 bp, and further sequencing results showed that the sequence differences were mainly concentrated in ITS1 and ITS2 region. [Conclusions] Systematic evaluation for the identification of P. capitatum is established, which combines with macroscopic identification, physicochemical identification, powder microscope identification, and DNA molecular identification. Finally, the original medicinal material is identified as P. capitatum Buch.-Ham. ex D. Don.展开更多
Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and pa...Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and patient stratification.Here,we conducted genomic and transcriptomic profiling of 203 patients from the ECTOP-2002 study with full clinical information.Mutation in the mucin family,as well as APOBEC signature,were associated with poor prognosis.In contrast,activation of the epithelial-keratinization(EpK)pathway was strongly linked to favorable prognosis and lower post-chemotherapy recurrence rates.Independent validation supported S100A8+S100A9 complex as a key marker of EpK pathway.Furthermore,we established a prognostic stratification system,FU-ESCC subtyping,which defines three subtypes with distinct molecular and clinical features.The EpK-activated subtype retained characteristics of healthy squamous epithelial cells,showed high expression of the S100A8+S100A9 complex,and was associated with favorable prognosis.展开更多
To the Editor:Stroke is among the leading public health problems in China,with high rates of recurrent stroke,cardiovascular events and mortality,resulting in a considerable medical and economic burden.[1]The identifi...To the Editor:Stroke is among the leading public health problems in China,with high rates of recurrent stroke,cardiovascular events and mortality,resulting in a considerable medical and economic burden.[1]The identification of new molecular markers and therapeutic targets is necessary to better refine the risk prediction of cardiovascular events and develop new treatment strategies for ischemic stroke.Succinate is a tricarboxylic acid(TCA)cycle intermediate and has received considerable attention for its involvement in various signaling pathways associated with cardiovascular diseases.[2–4]Recent studies have reported that succinate is a novel mediator that promotes atherosclerotic lesion progression,fibroblast activation,and apoptosis resistance.展开更多
文摘The wild cotton cultivars and species have abundant genetic polymorphisms,and they possess lots of excellent genes,such as drought resistance,insect resistance,fine and strong fiber,and so on.
文摘Marker-aided selection has received more attention in recent years. This relies on the exploitation of dose linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAPD) marker tightly linked to the blast resistance gene Pi-ll(t) derived from Hongjiaozhan, which confers the resistance to race ZB1 of Pyricularia oryzae Cav.
基金Supported by Guizhou Provincial Science and Technology Project[ZK(2022)-362](2022)4028+5 种基金ZK(2021)-554ZK(2023)-378]Science Foundation of Guizhou Health Commission(gzwkj2021-449)Innovation and Entrepreneurship Training Program for Undergraduates from China(202210660131)Science Foundation of Guizhou Education Technology(2022-064)Rural Economic Revitalization Research Project of Guizhou Medical University(GZYKDX-2022-002).
文摘[Objectives] To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum . [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical identification was conducted on plant roots, stems, leaves, flowers and fruits. The P. capitatum powder was processed for physical and chemical distinction by FeCl 3 chromogenic reaction, hydrochloric acid magnesium powder reaction, AlCl 3 color development reaction and thin-layer chromatography.Microscope identification was carried out on the powder. Plant genome DNeasy Plant Kit was adopted for DNA molecular marker identification. [Results] The results showed that the stem of P. capitatum was tufted, the leaves were oval, 2 to 5 cm long, and 1 to 2 cm wide;the leaf apex was acute and cuneate at the base, the inflorescence was capitate, paired or solitary;the raceme was erect and nearly spherical, and the perianth was light red. Furthermore, for the chromogenic reaction of FeCl 3 ethanol extract of P. capitatum , appeared blue and turned to dark blue after long time storing at room temperature. For the reaction of hydrochloric acid magnesium powder, the alcohol extract of P. capitatum , exhibited deep red. In the color reaction of AlCl 3, the alcohol extract revealed yellow fluorescence under 360 nm UV lamp. Microscope identification of the powder displayed pollen grains, crystal sheath fibers, cellulose, vessels, starch grains, cork cells, and other characteristic fragments. In addition, DNA barcoding electrophoresis results showed that P. capitatum showed a clear and bright single band near 500 bp, and further sequencing results showed that the sequence differences were mainly concentrated in ITS1 and ITS2 region. [Conclusions] Systematic evaluation for the identification of P. capitatum is established, which combines with macroscopic identification, physicochemical identification, powder microscope identification, and DNA molecular identification. Finally, the original medicinal material is identified as P. capitatum Buch.-Ham. ex D. Don.
基金supported by National Key R&D Program of China(2022YFA1103900)National Natural Science Foundation of China(82430099)+1 种基金the Clinical Research Special Project of Shanghai Municipal Health Commission(202440070)the Medical Research Special Project for Shanghai Science and Technology Innovation Action Plan(24Y12800400).
文摘Recent large-scale multi-omics studies have characterized the heterogeneity of esophageal squamous cell carcinoma(ESCC),but inconsistent clinical management has hindered the identification of prognostic markers and patient stratification.Here,we conducted genomic and transcriptomic profiling of 203 patients from the ECTOP-2002 study with full clinical information.Mutation in the mucin family,as well as APOBEC signature,were associated with poor prognosis.In contrast,activation of the epithelial-keratinization(EpK)pathway was strongly linked to favorable prognosis and lower post-chemotherapy recurrence rates.Independent validation supported S100A8+S100A9 complex as a key marker of EpK pathway.Furthermore,we established a prognostic stratification system,FU-ESCC subtyping,which defines three subtypes with distinct molecular and clinical features.The EpK-activated subtype retained characteristics of healthy squamous epithelial cells,showed high expression of the S100A8+S100A9 complex,and was associated with favorable prognosis.
基金This study was supported by the National Natural Science Foundation of China(Nos.82273706 and 82220108001)the Suzhou Science and Technology Project(No.SYW2024067)+1 种基金Interdisciplinary Basic Frontier Innovation Program of Suzhou Medical College of Soochow University(No.YXY2302013)the Project of MOE Key Laboratory of Geriatric Diseases and Immunology(No.JYN202406).
文摘To the Editor:Stroke is among the leading public health problems in China,with high rates of recurrent stroke,cardiovascular events and mortality,resulting in a considerable medical and economic burden.[1]The identification of new molecular markers and therapeutic targets is necessary to better refine the risk prediction of cardiovascular events and develop new treatment strategies for ischemic stroke.Succinate is a tricarboxylic acid(TCA)cycle intermediate and has received considerable attention for its involvement in various signaling pathways associated with cardiovascular diseases.[2–4]Recent studies have reported that succinate is a novel mediator that promotes atherosclerotic lesion progression,fibroblast activation,and apoptosis resistance.
