毛竹林主要分布在缺磷的亚热带地区,具有天然的立竹年龄结构及独特的篼根和鞭根“双根系统”,挖掘根际解磷菌种资源并探明其对磷添加的响应,是提升毛竹生产力和生态功能的关键。本研究采用微孔板高通量筛选方法,分离3个磷添加水平(对照,...毛竹林主要分布在缺磷的亚热带地区,具有天然的立竹年龄结构及独特的篼根和鞭根“双根系统”,挖掘根际解磷菌种资源并探明其对磷添加的响应,是提升毛竹生产力和生态功能的关键。本研究采用微孔板高通量筛选方法,分离3个磷添加水平(对照,CK,0 kg P·hm^(-2)·a^(-1);低磷,LP,50 kg P·hm^(-2)·a^(-1);高磷,HP,100 kg P·hm^(-2)·a^(-1))样地的毛竹篼根(1龄和3龄)及相连鞭根根际解磷细菌,探究解磷菌数量与活性对磷添加的响应及其影响因素。毛竹根际共分离出125株解磷菌,其中96.8%为变形菌门,主要包括布鲁氏菌属和伯克霍尔德氏菌属。3龄竹篼根的解磷菌最多(52株),解磷活性为171.24 mg·L^(-1),分别是1龄竹篼根和鞭根解磷菌解磷活性的1.5和1.4倍。与对照样地相比,LP样地1龄竹篼根解磷菌的数量和活性均降低,HP样地解磷菌的数量保持不变但活性增加;LP样地3龄竹篼根和鞭根的根际解磷菌的数量降低但解磷活性增加,HP样地解磷菌的数量和活性均增加。细菌的解磷活性与土壤总磷、pH和微生物生物量磷呈显著正相关。综上,3龄毛竹篼根解磷菌的数量和活性均高于1龄竹篼根和鞭根,并对磷添加有积极响应。建议在毛竹林集约化管理中优先施磷肥于3龄竹篼根,以更大限度地激活土壤微生物的解磷潜力。展开更多
[Objective] The Cu-tolerance and Cu enrichment capacity of TLSB2-K were investigated. [Method] The previously isolated TLSB2-K strain was identified though morphological observation, gram staining and 16S rDNA sequenc...[Objective] The Cu-tolerance and Cu enrichment capacity of TLSB2-K were investigated. [Method] The previously isolated TLSB2-K strain was identified though morphological observation, gram staining and 16S rDNA sequence alignment. In ad- dition, the effects of temperature, pH and osmotic pressure on the growth of strain were also investigated by using shaking culture. The Cu-tolerance and Cu enrich- ment capacity of TLSB2-K strain under Cu stress were also studied. [Result] The results showed TLSB2-K belongs to Bacillus spp., and its optimum growth conditions were as follows： temperature, 27 ~C; pH, 7.0; osmotic pressure, 1.1% NaCI. When the Cu concentration ranged from 100 mg/L to 500 mg/L, the strain grew well; when the Cu concentration ranged from 100 mg/L to 400 mg/L, the Cu content in bacteria was increased with the increase of Cu concentration; when the Cu con- centration was 400 mg/L and the incubation time was 30 h, the Cu content in bac- teria reached the peak （2 250 mg/kg）; the highest tolerant concentration was 700 mg/L. [Conclusion] TLSB2-K strain had relatively high Cu tolerance and Cu enrich- ment capacity. They had important theoretical research and engineering application values.展开更多
In the research, changes of apple chemistry, and molecule, under stresses, are n terms of morphology, physiology, bio- illustrated and research and identifica- tion methods of apple resistance are explored involving ...In the research, changes of apple chemistry, and molecule, under stresses, are n terms of morphology, physiology, bio- illustrated and research and identifica- tion methods of apple resistance are explored involving drought-resistance, flood-re- sistance, salt-stress resistance, cold-hardiness and heat-resistance. In addition prospects of apple resistance research are proposed, as well.展开更多
文摘毛竹林主要分布在缺磷的亚热带地区,具有天然的立竹年龄结构及独特的篼根和鞭根“双根系统”,挖掘根际解磷菌种资源并探明其对磷添加的响应,是提升毛竹生产力和生态功能的关键。本研究采用微孔板高通量筛选方法,分离3个磷添加水平(对照,CK,0 kg P·hm^(-2)·a^(-1);低磷,LP,50 kg P·hm^(-2)·a^(-1);高磷,HP,100 kg P·hm^(-2)·a^(-1))样地的毛竹篼根(1龄和3龄)及相连鞭根根际解磷细菌,探究解磷菌数量与活性对磷添加的响应及其影响因素。毛竹根际共分离出125株解磷菌,其中96.8%为变形菌门,主要包括布鲁氏菌属和伯克霍尔德氏菌属。3龄竹篼根的解磷菌最多(52株),解磷活性为171.24 mg·L^(-1),分别是1龄竹篼根和鞭根解磷菌解磷活性的1.5和1.4倍。与对照样地相比,LP样地1龄竹篼根解磷菌的数量和活性均降低,HP样地解磷菌的数量保持不变但活性增加;LP样地3龄竹篼根和鞭根的根际解磷菌的数量降低但解磷活性增加,HP样地解磷菌的数量和活性均增加。细菌的解磷活性与土壤总磷、pH和微生物生物量磷呈显著正相关。综上,3龄毛竹篼根解磷菌的数量和活性均高于1龄竹篼根和鞭根,并对磷添加有积极响应。建议在毛竹林集约化管理中优先施磷肥于3龄竹篼根,以更大限度地激活土壤微生物的解磷潜力。
基金Supported by Hubei Provincial Department of Education Youth Found(Q2014402)Key Project of Natural Science Foundation of Hubei Province(2011CDA037)+2 种基金Special Fund for Agro-scientific Research in the Public Interest of Hubei Province(2012DCA23)Hubei Provincial Key Laboratory of Mining Environmental Pollution Control and Remediation Open Foundation(2012106)Project of Outstanding Yong and Middleaged Scientific and Technological Innovation Team of Colleges and Universities in Hubei Province(T201223)~~
文摘[Objective] The Cu-tolerance and Cu enrichment capacity of TLSB2-K were investigated. [Method] The previously isolated TLSB2-K strain was identified though morphological observation, gram staining and 16S rDNA sequence alignment. In ad- dition, the effects of temperature, pH and osmotic pressure on the growth of strain were also investigated by using shaking culture. The Cu-tolerance and Cu enrich- ment capacity of TLSB2-K strain under Cu stress were also studied. [Result] The results showed TLSB2-K belongs to Bacillus spp., and its optimum growth conditions were as follows： temperature, 27 ~C; pH, 7.0; osmotic pressure, 1.1% NaCI. When the Cu concentration ranged from 100 mg/L to 500 mg/L, the strain grew well; when the Cu concentration ranged from 100 mg/L to 400 mg/L, the Cu content in bacteria was increased with the increase of Cu concentration; when the Cu con- centration was 400 mg/L and the incubation time was 30 h, the Cu content in bac- teria reached the peak （2 250 mg/kg）; the highest tolerant concentration was 700 mg/L. [Conclusion] TLSB2-K strain had relatively high Cu tolerance and Cu enrich- ment capacity. They had important theoretical research and engineering application values.
基金Supported by Shandong Provincial Natural Science Foundation in China(ZR2011CM034)~~
文摘In the research, changes of apple chemistry, and molecule, under stresses, are n terms of morphology, physiology, bio- illustrated and research and identifica- tion methods of apple resistance are explored involving drought-resistance, flood-re- sistance, salt-stress resistance, cold-hardiness and heat-resistance. In addition prospects of apple resistance research are proposed, as well.
