Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethyl...Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)method.Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold-based immunochromatographic assay(CG-ICA)methods were used to determine R2G in fruit drinks,red wine,and yoghurts.A standard curve of the developed ic-ELISA showed that the IC50 of the anti-R2G mAb was 1.02 ng/mL,and limit of detection value(LOD)was 0.21 ng/mL.For the CG-ICA developed,the visual limit of detection values(vLOD)were 2 ng/mL and cut-off values of 100 ng/mL in samples.The results indicated that these two methods could be used to quickly detect R2G in fruit drinks,red wine,and yoghurts.展开更多
基金This work was funded by the National Key R&D Program(2019YFC1604602)grants from Science and Technology Program of Jiangsu Market Supervision and Administration Bureau(KJ21125093)This work was also supported by the Fundamental Research Funds for the Central Universities(JUSRP622012).
文摘Red 2G(R2G),a cheap industrial colorant,cannot be added to food.An anti-R2G monoclonal antibody(mAb)was prepared by immunizing mice with the conjugate of R2G hapten and protein,which based on the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC)method.Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)and colloidal gold-based immunochromatographic assay(CG-ICA)methods were used to determine R2G in fruit drinks,red wine,and yoghurts.A standard curve of the developed ic-ELISA showed that the IC50 of the anti-R2G mAb was 1.02 ng/mL,and limit of detection value(LOD)was 0.21 ng/mL.For the CG-ICA developed,the visual limit of detection values(vLOD)were 2 ng/mL and cut-off values of 100 ng/mL in samples.The results indicated that these two methods could be used to quickly detect R2G in fruit drinks,red wine,and yoghurts.
