目的开发和评估基于深度学习算法的自动识别角膜共聚焦显微镜(in vivo confocal microscopy,IVCM)图片中角膜炎细胞的智能辅助诊断系统。方法纳入广西壮族自治区人民医院眼科感染性角膜炎患者IVCM图像。采用ResNet101卷积神经网络构建...目的开发和评估基于深度学习算法的自动识别角膜共聚焦显微镜(in vivo confocal microscopy,IVCM)图片中角膜炎细胞的智能辅助诊断系统。方法纳入广西壮族自治区人民医院眼科感染性角膜炎患者IVCM图像。采用ResNet101卷积神经网络构建智能模型。使用5倍交叉验证的方法对模型的效能进行检验,计算模型准确度、特异度和敏感度评估该智能辅助诊断系统的识别真菌菌丝、炎症细胞、活化的树突细胞的效能。结果该研究共纳入2105张图片,经交叉验证,该模型识别真菌菌丝的准确度为0.974,特异度为0.976,敏感度为0.971。识别炎症细胞的准确度为0.993,特异度为0.994,敏感度为0.990。识别活化的树突细胞的准确度为0.993,特异度为0.994,敏感度为0.990。结论该研究自主研发的基于深度学习算法的智能系统可有效地将共聚焦图片中的角膜炎异常细胞进行自动识别,在识别多种IVCM图像的角膜炎细胞中表现出良好的诊断效能。展开更多
Purpose:Assessing the quality of the ocular surface by in vivo scanning laser confocal microscopy(IVCM)in primary open angle glaucoma(POAG)patients treated by Xen 45 Gel Stent,medical therapy and trabeculectomy.Method...Purpose:Assessing the quality of the ocular surface by in vivo scanning laser confocal microscopy(IVCM)in primary open angle glaucoma(POAG)patients treated by Xen 45 Gel Stent,medical therapy and trabeculectomy.Methods:Retrospective,single-center,single-masked,comparative study including 60 eyes of 30 patients(mean age 61.16±10 years)affected by POAG.Eyes were divided into 3 groups:Group 1 eyes underwent the Xen 45 Gel Stent procedure,Group 2 eyes were under medical therapy,Group 3 eyes were surgically treated by trabeculectomy.All patients underwent HRT II IVCM analysis of cornea,limbus,conjunctiva,sub-tenionian space and sclera.Results:The Xen 45 Gel stent,if properly positioned in the sub-conjunctival space preserves goblet cells and limits ocular surface inflammation.Regular corneal epithelial cells with micro-cysts,and normo-reflective sub-epithelial nerve plexus are documented by IVCM.In sub Tenon’s implants an alternative lamellar intra-scleral filtration is detectable.Combined surgical procedures show a noticeable number of inflammatory cells with rare micro-cysts.Post-trabeculectomy inflammatory reaction is more evident than Xen 45 Gel Stent associated surgical procedures,but less than medical therapy where a conspicuous presence of Langerhans cells,peri-neural infiltrates,marked loss of goblet cells and fibrosis is visible.Conclusion:Ocular surface inflammation was more notable in topical therapy than after trabeculectomy,which itself causes more inflammation than XEN Gel stents.展开更多
文摘目的开发和评估基于深度学习算法的自动识别角膜共聚焦显微镜(in vivo confocal microscopy,IVCM)图片中角膜炎细胞的智能辅助诊断系统。方法纳入广西壮族自治区人民医院眼科感染性角膜炎患者IVCM图像。采用ResNet101卷积神经网络构建智能模型。使用5倍交叉验证的方法对模型的效能进行检验,计算模型准确度、特异度和敏感度评估该智能辅助诊断系统的识别真菌菌丝、炎症细胞、活化的树突细胞的效能。结果该研究共纳入2105张图片,经交叉验证,该模型识别真菌菌丝的准确度为0.974,特异度为0.976,敏感度为0.971。识别炎症细胞的准确度为0.993,特异度为0.994,敏感度为0.990。识别活化的树突细胞的准确度为0.993,特异度为0.994,敏感度为0.990。结论该研究自主研发的基于深度学习算法的智能系统可有效地将共聚焦图片中的角膜炎异常细胞进行自动识别,在识别多种IVCM图像的角膜炎细胞中表现出良好的诊断效能。
文摘Purpose:Assessing the quality of the ocular surface by in vivo scanning laser confocal microscopy(IVCM)in primary open angle glaucoma(POAG)patients treated by Xen 45 Gel Stent,medical therapy and trabeculectomy.Methods:Retrospective,single-center,single-masked,comparative study including 60 eyes of 30 patients(mean age 61.16±10 years)affected by POAG.Eyes were divided into 3 groups:Group 1 eyes underwent the Xen 45 Gel Stent procedure,Group 2 eyes were under medical therapy,Group 3 eyes were surgically treated by trabeculectomy.All patients underwent HRT II IVCM analysis of cornea,limbus,conjunctiva,sub-tenionian space and sclera.Results:The Xen 45 Gel stent,if properly positioned in the sub-conjunctival space preserves goblet cells and limits ocular surface inflammation.Regular corneal epithelial cells with micro-cysts,and normo-reflective sub-epithelial nerve plexus are documented by IVCM.In sub Tenon’s implants an alternative lamellar intra-scleral filtration is detectable.Combined surgical procedures show a noticeable number of inflammatory cells with rare micro-cysts.Post-trabeculectomy inflammatory reaction is more evident than Xen 45 Gel Stent associated surgical procedures,but less than medical therapy where a conspicuous presence of Langerhans cells,peri-neural infiltrates,marked loss of goblet cells and fibrosis is visible.Conclusion:Ocular surface inflammation was more notable in topical therapy than after trabeculectomy,which itself causes more inflammation than XEN Gel stents.
