The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The mai...The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The main distribution area of the C. angulata is located in Fujian, South China. In total, 420 C. angulata were collected from 14 natural habitats(populations) along the Fujian coast, and their genetic diversity and structure were analyzed in the mitochondrial COI and nuclear gene ITS2 sequences. Results reveal that all the 14 populations of C. angulata exhibited high levels of genetic diversity, with a total of 57(haplotype diversity: 0.811±0.016) and 124(haplotype diversity: 0.912±0.007) haplotypes revealed by COI and ITS2, respectively. Notably, significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ) population(FS T by COI: 0.035–0.142, P<0.05;FS T by ITS2: 0.078–0.123, P<0.05) with other populations were observed for the first time, which is also supported by the results of molecular variance analysis(FC T by COI: 0.105, P<0.05;FC T by ITS2: 0.086, P<0.05) and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree. Furthermore, the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations. In addition, the population dynamics analysis suggests that the C. angulata may have undergone expansion during the third ice age of the Pleistocene. These results provide a reference for the preservation and further genetic improvement of C. angulata.展开更多
[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amp...[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amplified using universal primers to obtain ITS2 sequences of seven Xanthium species, which were then sequenced. ITS2 sequences of seven Xanthium species were compared and analyzed by MEGA 5.1 software, and the phylogenetic tree was constructed. [ Result] A total of 242 variable sites were found in 1TS2 sequences of Xanthium species, including 12 singleton variable sites. [ Conclusion] Xanthium species can be distinguished and identified based on ITS2 sequences.展开更多
基金Supported by the National Natural Science Foundation of China(No.32172979)the Natural Science Foundation of Fujian Province(No.2021J05159)the 2023 Special Program for Promoting High-Quality Development of Marine and Fishery Industry in Fujian Province(No.PJHYF-L-2023-2)。
文摘The Fujian oyster(Crassostrea angulata) is an economically significant shellfish species distributed mainly along the Fujian coast, Southeast China. However, its genetic diversity and structure remain unclear. The main distribution area of the C. angulata is located in Fujian, South China. In total, 420 C. angulata were collected from 14 natural habitats(populations) along the Fujian coast, and their genetic diversity and structure were analyzed in the mitochondrial COI and nuclear gene ITS2 sequences. Results reveal that all the 14 populations of C. angulata exhibited high levels of genetic diversity, with a total of 57(haplotype diversity: 0.811±0.016) and 124(haplotype diversity: 0.912±0.007) haplotypes revealed by COI and ITS2, respectively. Notably, significant intermediate level of genetic differentiations between the Ningde Zhujiang(ZJ) population(FS T by COI: 0.035–0.142, P<0.05;FS T by ITS2: 0.078–0.123, P<0.05) with other populations were observed for the first time, which is also supported by the results of molecular variance analysis(FC T by COI: 0.105, P<0.05;FC T by ITS2: 0.086, P<0.05) and the clustering of the ZJ population into distinct branches in the interpopulation genetic differentiation tree. Furthermore, the evolutionary tree and haplotype network analyses do not support the formation of a clear geographical genealogical structure among these 14 populations. In addition, the population dynamics analysis suggests that the C. angulata may have undergone expansion during the third ice age of the Pleistocene. These results provide a reference for the preservation and further genetic improvement of C. angulata.
基金Supported by Science and Technology Project of Jiangsu Entry-Exit Inspection and Quarantine Bureau(2012KJ54)
文摘[ Objective ] Xanthium plants （non-Chinese species） are quarantine pests in China. This study aimed to distinguish and identify seven Xanthium spe- cies using ITS2 sequence as DNA barcode. [Method] ITS2 gene was amplified using universal primers to obtain ITS2 sequences of seven Xanthium species, which were then sequenced. ITS2 sequences of seven Xanthium species were compared and analyzed by MEGA 5.1 software, and the phylogenetic tree was constructed. [ Result] A total of 242 variable sites were found in 1TS2 sequences of Xanthium species, including 12 singleton variable sites. [ Conclusion] Xanthium species can be distinguished and identified based on ITS2 sequences.
