Objective Acquired aplastic anemia(aAA)is characterized by an autologous immunological attack against hematopoietic stem and progenitor cells,and immunotolerance disruption is frequent,with reduced T regulatory cells(...Objective Acquired aplastic anemia(aAA)is characterized by an autologous immunological attack against hematopoietic stem and progenitor cells,and immunotolerance disruption is frequent,with reduced T regulatory cells(Tregs)frequencies and increased effector cytotoxic cells.Tregs are reduced in aAA and increase in number after successful immunosuppressive therapies.Methods In this retrospective study,we investigated the frequency of circulating Tregs by multiparametric flow cytometry immunophenotyping in non-severe aAA patients before and after immunosuppressive therapy.The samples were stained with the following antibodies:ECD anti-CD3,PE or PC5 anti-CD4,FITC anti-CD8,and PE anti-CD25,and Tregs were identified by first gating on linear parameters for lymphocyte identification and then for CD3 expression.In CD3+CD4+cells,Tregs were further identified on the basis of CD25 and FOXP3 expression.Results Although the number of Tregs tended to increase after immunosuppressive treatments,their circulating frequency remained lower than that of healthy subjects,regardless of their responsiveness to therapies.Moreover,the relative frequency combined with absolute Treg counts might be more informative in the differential diagnosis of bone marrow failure syndromes.Conclusions The persistent decrease in circulating Tregs could be the result of immunosuppressive agents that could preferentially expand other T-cell subsets.At the same time,an imbalance in immunotolerance might persist,which is also favored by chronic antigen stimulation.展开更多
ISTE为了更好地支持有效利用技术进行学习,为教育工作者和学校领导提供了一个框架——《ISTE基本条件》(The ISTE Essential Conditions)。《ISTE基本条件》框架给出了信息技术有效融入教学所必需的14个关键要素。本文全面系统地介绍了...ISTE为了更好地支持有效利用技术进行学习,为教育工作者和学校领导提供了一个框架——《ISTE基本条件》(The ISTE Essential Conditions)。《ISTE基本条件》框架给出了信息技术有效融入教学所必需的14个关键要素。本文全面系统地介绍了《ISTE基本条件》框架基本内容,并将《ISTE基本条件》与《微软K-12教育变革框架》进行比较,指《ISTE基本条件》对我国教育信息化发展具有一定的启发和借鉴意义。展开更多
《中国粉体技术》是中国科学院中国科学引文数据库(CSCD)收录期刊、科技期刊世界影响力指数报告(WJCI)收录期刊、中国科学技术信息研究所中国科技核心期刊(CSTPCD)、武汉大学中国核心学术期刊(RCCSE)和中国应用型核心期刊(CACJ),以及美...《中国粉体技术》是中国科学院中国科学引文数据库(CSCD)收录期刊、科技期刊世界影响力指数报告(WJCI)收录期刊、中国科学技术信息研究所中国科技核心期刊(CSTPCD)、武汉大学中国核心学术期刊(RCCSE)和中国应用型核心期刊(CACJ),以及美国化学文摘(CA)、日本科学技术振兴机构数据库(IST)、欧洲学术出版中心数据库(EuroPub)、波兰哥白尼索引数据库(ICI World of Journals)、乌利希国际期刊数据库(Ulrichsweb)、哥白尼精选期刊数据库(ICI journals Master List)、开放获取期刊数据库(DOAJ)等收录期刊。荣获中国高校优秀科技期刊、山东省高校学报优秀期刊、Caj-CD规范获奖期刊、中国建筑材料联合会建材行业先进媒体、博看出版融合创新奖等荣誉称号。展开更多
文摘Objective Acquired aplastic anemia(aAA)is characterized by an autologous immunological attack against hematopoietic stem and progenitor cells,and immunotolerance disruption is frequent,with reduced T regulatory cells(Tregs)frequencies and increased effector cytotoxic cells.Tregs are reduced in aAA and increase in number after successful immunosuppressive therapies.Methods In this retrospective study,we investigated the frequency of circulating Tregs by multiparametric flow cytometry immunophenotyping in non-severe aAA patients before and after immunosuppressive therapy.The samples were stained with the following antibodies:ECD anti-CD3,PE or PC5 anti-CD4,FITC anti-CD8,and PE anti-CD25,and Tregs were identified by first gating on linear parameters for lymphocyte identification and then for CD3 expression.In CD3+CD4+cells,Tregs were further identified on the basis of CD25 and FOXP3 expression.Results Although the number of Tregs tended to increase after immunosuppressive treatments,their circulating frequency remained lower than that of healthy subjects,regardless of their responsiveness to therapies.Moreover,the relative frequency combined with absolute Treg counts might be more informative in the differential diagnosis of bone marrow failure syndromes.Conclusions The persistent decrease in circulating Tregs could be the result of immunosuppressive agents that could preferentially expand other T-cell subsets.At the same time,an imbalance in immunotolerance might persist,which is also favored by chronic antigen stimulation.
文摘ISTE为了更好地支持有效利用技术进行学习,为教育工作者和学校领导提供了一个框架——《ISTE基本条件》(The ISTE Essential Conditions)。《ISTE基本条件》框架给出了信息技术有效融入教学所必需的14个关键要素。本文全面系统地介绍了《ISTE基本条件》框架基本内容,并将《ISTE基本条件》与《微软K-12教育变革框架》进行比较,指《ISTE基本条件》对我国教育信息化发展具有一定的启发和借鉴意义。
文摘《中国粉体技术》是中国科学院中国科学引文数据库(CSCD)收录期刊、科技期刊世界影响力指数报告(WJCI)收录期刊、中国科学技术信息研究所中国科技核心期刊(CSTPCD)、武汉大学中国核心学术期刊(RCCSE)和中国应用型核心期刊(CACJ),以及美国化学文摘(CA)、日本科学技术振兴机构数据库(IST)、欧洲学术出版中心数据库(EuroPub)、波兰哥白尼索引数据库(ICI World of Journals)、乌利希国际期刊数据库(Ulrichsweb)、哥白尼精选期刊数据库(ICI journals Master List)、开放获取期刊数据库(DOAJ)等收录期刊。荣获中国高校优秀科技期刊、山东省高校学报优秀期刊、Caj-CD规范获奖期刊、中国建筑材料联合会建材行业先进媒体、博看出版融合创新奖等荣誉称号。
