西安交通大学机械学院为了探索如何更好地应对新形势下的国际化高等教育挑战,在全英文“机械表面工程概论”课程中提出了一种全新概念的教学模式——ISG(Integrated Small Groups)。该模式深入融合国际及中国学生,强调跨文化合作与交流...西安交通大学机械学院为了探索如何更好地应对新形势下的国际化高等教育挑战,在全英文“机械表面工程概论”课程中提出了一种全新概念的教学模式——ISG(Integrated Small Groups)。该模式深入融合国际及中国学生,强调跨文化合作与交流,以促进不同背景学生的学术合作和文化理解。ISG教学模式通过外籍学生与中国学生结组,以工程问题为导引,理论讲授和科研实践相结合的形式开展。教学中采用全英文授课,帮助学生克服文化及语言习惯差异,同时采用多样化的教学方法,帮助学生适应课程学习节奏。课程的ISG教学模式对于应对国际化高等教育挑战有着重要意义。展开更多
Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon...Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon-stimulated genes(ISGs),including PKR,OAS1,MX1,and ISG15,in peripheral blood mononuclear cells of 45 COVID-19 patients with different severities were evaluated by real-time PCR method.Results:OAS1,MX1,PKR,and ISG15 were differently expressed in COVID-19 patients with different severity.The results showed that the expression of OAS1,MX1,PKR,and ISG15 genes was significantly(P=0.001)lower in severe patients.Conclusions:Weak and defective IFN response and subsequent disruption of ISGs may be associated with COVID-19 severity.展开更多
Nine new germacranolides,sylvaticalides A−H(1-9),and three known analogues(10-12)were isolated from the aeri-al part of Vernonia sylvatica.Their structures were established using comprehensive spectroscopic analysis,i...Nine new germacranolides,sylvaticalides A−H(1-9),and three known analogues(10-12)were isolated from the aeri-al part of Vernonia sylvatica.Their structures were established using comprehensive spectroscopic analysis,including high-resolution electrospray ionization mass spectroscopy(HR-ESI-MS)and 1D and 2D nuclear magnetic resonance(NMR)spectra.Their absolute configurations were determined by X-ray diffraction experiments.The anti-inflammatory activities of all isolated compounds were as-sessed by evaluating their inhibitory effects on the nuclear factor kappa B(NF-κB)pathway,which was activated by lipopolysacchar-ide(LPS)-stimulated human THP1-Dual cells,and the interferon-stimulated gene(ISG)pathway,activated by STING agonist MSA-2 in the same cell model.Compounds 1,2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways,with IC_(50)values ranging from 4.12 to 10.57μmol·L^(−1).展开更多
Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed g...Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed genes in the psoriasis dataset,identify the central genes,detect the expression of central genes in psoriasis lesions of patients in the cold regions and then conduct further research.Methods:Differential genes associated with psoriasis in the GEO database were analyzed,and functional enrichment analysis and protein-protein interaction network analysis.The expression results of the identified genes were validated in psoriasis cell models.The ISG15 gene,which showed the most significant difference in expression,was further studied.The expression level of ISG15 protein in psoriasis was examined.Then,we knocked out ISG15 in psoriasis cell models and detected keratinocyte proliferation by MTT,Real-Time PCR and Western Blot.Western Blot showed the expression ofβ-catenin after ISG15 gene knockout.Results:We detected the protein expression of ISG15 in the cold area of Northeast China,and found that the expression of ISG15 increased in patients with psoriasis,and the proliferation of keratinocytes and the expression ofβ-catenin decreased in psoriasis cell model after ISG15 was knocked down.ISG15 regulates keratinocyte proliferation through Wnt signaling pathway in psoriasis.Conclusions:ISG15 expression is increased in psoriatic cells and skin lesions of patients with psoriasis.In psoriasis,ISG15 promotes keratinocyte proliferation through the Wnt signaling pathway.展开更多
文摘西安交通大学机械学院为了探索如何更好地应对新形势下的国际化高等教育挑战,在全英文“机械表面工程概论”课程中提出了一种全新概念的教学模式——ISG(Integrated Small Groups)。该模式深入融合国际及中国学生,强调跨文化合作与交流,以促进不同背景学生的学术合作和文化理解。ISG教学模式通过外籍学生与中国学生结组,以工程问题为导引,理论讲授和科研实践相结合的形式开展。教学中采用全英文授课,帮助学生克服文化及语言习惯差异,同时采用多样化的教学方法,帮助学生适应课程学习节奏。课程的ISG教学模式对于应对国际化高等教育挑战有着重要意义。
基金the research council of Kerman University of Medical Sciences,Kerman,Iran(Grant Number:400000232).
文摘Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon-stimulated genes(ISGs),including PKR,OAS1,MX1,and ISG15,in peripheral blood mononuclear cells of 45 COVID-19 patients with different severities were evaluated by real-time PCR method.Results:OAS1,MX1,PKR,and ISG15 were differently expressed in COVID-19 patients with different severity.The results showed that the expression of OAS1,MX1,PKR,and ISG15 genes was significantly(P=0.001)lower in severe patients.Conclusions:Weak and defective IFN response and subsequent disruption of ISGs may be associated with COVID-19 severity.
文摘Nine new germacranolides,sylvaticalides A−H(1-9),and three known analogues(10-12)were isolated from the aeri-al part of Vernonia sylvatica.Their structures were established using comprehensive spectroscopic analysis,including high-resolution electrospray ionization mass spectroscopy(HR-ESI-MS)and 1D and 2D nuclear magnetic resonance(NMR)spectra.Their absolute configurations were determined by X-ray diffraction experiments.The anti-inflammatory activities of all isolated compounds were as-sessed by evaluating their inhibitory effects on the nuclear factor kappa B(NF-κB)pathway,which was activated by lipopolysacchar-ide(LPS)-stimulated human THP1-Dual cells,and the interferon-stimulated gene(ISG)pathway,activated by STING agonist MSA-2 in the same cell model.Compounds 1,2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways,with IC_(50)values ranging from 4.12 to 10.57μmol·L^(−1).
基金supported by the Natural Science Foundation of Heilongjiang Province(PL2024H107).
文摘Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed genes in the psoriasis dataset,identify the central genes,detect the expression of central genes in psoriasis lesions of patients in the cold regions and then conduct further research.Methods:Differential genes associated with psoriasis in the GEO database were analyzed,and functional enrichment analysis and protein-protein interaction network analysis.The expression results of the identified genes were validated in psoriasis cell models.The ISG15 gene,which showed the most significant difference in expression,was further studied.The expression level of ISG15 protein in psoriasis was examined.Then,we knocked out ISG15 in psoriasis cell models and detected keratinocyte proliferation by MTT,Real-Time PCR and Western Blot.Western Blot showed the expression ofβ-catenin after ISG15 gene knockout.Results:We detected the protein expression of ISG15 in the cold area of Northeast China,and found that the expression of ISG15 increased in patients with psoriasis,and the proliferation of keratinocytes and the expression ofβ-catenin decreased in psoriasis cell model after ISG15 was knocked down.ISG15 regulates keratinocyte proliferation through Wnt signaling pathway in psoriasis.Conclusions:ISG15 expression is increased in psoriatic cells and skin lesions of patients with psoriasis.In psoriasis,ISG15 promotes keratinocyte proliferation through the Wnt signaling pathway.
