Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon...Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon-stimulated genes(ISGs),including PKR,OAS1,MX1,and ISG15,in peripheral blood mononuclear cells of 45 COVID-19 patients with different severities were evaluated by real-time PCR method.Results:OAS1,MX1,PKR,and ISG15 were differently expressed in COVID-19 patients with different severity.The results showed that the expression of OAS1,MX1,PKR,and ISG15 genes was significantly(P=0.001)lower in severe patients.Conclusions:Weak and defective IFN response and subsequent disruption of ISGs may be associated with COVID-19 severity.展开更多
Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed g...Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed genes in the psoriasis dataset,identify the central genes,detect the expression of central genes in psoriasis lesions of patients in the cold regions and then conduct further research.Methods:Differential genes associated with psoriasis in the GEO database were analyzed,and functional enrichment analysis and protein-protein interaction network analysis.The expression results of the identified genes were validated in psoriasis cell models.The ISG15 gene,which showed the most significant difference in expression,was further studied.The expression level of ISG15 protein in psoriasis was examined.Then,we knocked out ISG15 in psoriasis cell models and detected keratinocyte proliferation by MTT,Real-Time PCR and Western Blot.Western Blot showed the expression ofβ-catenin after ISG15 gene knockout.Results:We detected the protein expression of ISG15 in the cold area of Northeast China,and found that the expression of ISG15 increased in patients with psoriasis,and the proliferation of keratinocytes and the expression ofβ-catenin decreased in psoriasis cell model after ISG15 was knocked down.ISG15 regulates keratinocyte proliferation through Wnt signaling pathway in psoriasis.Conclusions:ISG15 expression is increased in psoriatic cells and skin lesions of patients with psoriasis.In psoriasis,ISG15 promotes keratinocyte proliferation through the Wnt signaling pathway.展开更多
基金the research council of Kerman University of Medical Sciences,Kerman,Iran(Grant Number:400000232).
文摘Objective:To explore expression level of interferon-stimulated genes PKR,OAS1,MX1,and ISG15 in peripheral blood mononuclear cells of COVID-19 patients.Methods:In this study,changes in the expression of four interferon-stimulated genes(ISGs),including PKR,OAS1,MX1,and ISG15,in peripheral blood mononuclear cells of 45 COVID-19 patients with different severities were evaluated by real-time PCR method.Results:OAS1,MX1,PKR,and ISG15 were differently expressed in COVID-19 patients with different severity.The results showed that the expression of OAS1,MX1,PKR,and ISG15 genes was significantly(P=0.001)lower in severe patients.Conclusions:Weak and defective IFN response and subsequent disruption of ISGs may be associated with COVID-19 severity.
基金supported by the Natural Science Foundation of Heilongjiang Province(PL2024H107).
文摘Objective:Psoriasis is a common chronic,recurrent,immune-mediated inflammatory skin disease,which tends to occur in cold areas.Its pathogenesis is currently unclear.This study aims to screen differentially expressed genes in the psoriasis dataset,identify the central genes,detect the expression of central genes in psoriasis lesions of patients in the cold regions and then conduct further research.Methods:Differential genes associated with psoriasis in the GEO database were analyzed,and functional enrichment analysis and protein-protein interaction network analysis.The expression results of the identified genes were validated in psoriasis cell models.The ISG15 gene,which showed the most significant difference in expression,was further studied.The expression level of ISG15 protein in psoriasis was examined.Then,we knocked out ISG15 in psoriasis cell models and detected keratinocyte proliferation by MTT,Real-Time PCR and Western Blot.Western Blot showed the expression ofβ-catenin after ISG15 gene knockout.Results:We detected the protein expression of ISG15 in the cold area of Northeast China,and found that the expression of ISG15 increased in patients with psoriasis,and the proliferation of keratinocytes and the expression ofβ-catenin decreased in psoriasis cell model after ISG15 was knocked down.ISG15 regulates keratinocyte proliferation through Wnt signaling pathway in psoriasis.Conclusions:ISG15 expression is increased in psoriatic cells and skin lesions of patients with psoriasis.In psoriasis,ISG15 promotes keratinocyte proliferation through the Wnt signaling pathway.
