The gene iscS-3 from ,4cidithiobacillus ferrooxidans may play a central role in the delivery of sulfur to a variety of metabolic pathways in this organism. For insight into the sulfur metabolic mechanism of the bacter...The gene iscS-3 from ,4cidithiobacillus ferrooxidans may play a central role in the delivery of sulfur to a variety of metabolic pathways in this organism. For insight into the sulfur metabolic mechanism of the bacteria, an integral three-dimensional （3D） molecular structure of the protein encoded by this gene was built by homology modeling techniques, refined by molecular dynamics simulations, assessed by PROFILE-3D and PROSTAT programs and further used to search bind sites, carry out flexible docking with cofactor pyridoxal 5＇-phosphate（PLP） and substrate cysteine and hereby detect its key residues. Through these procedures, the detail conformations of PLP-IscS（P-I） and cysteine-PLP-IscS（C-P-I） complexes were obtained. In P-I complex, the residues of Lys208, His106, Thr78, Ser205, His207, Asp182 and Gln185 have large interaction energies and/or hydrogen bonds fixation with PLP. In C-P-I complex, the amino group in cysteine is very near His106, Lys208 and PLP, the interaction energies for cysteine with them are very high. The above results are well consistent with those experimental facts of the homologues from other sources. Interestingly, the four residues of Glul05, Glu79, Ser203 and Hisl80 in P-I docking and the residue of Lys213 in C-P-I docking also have great interaction energies, which are fitly conservation in IscSs from all kinds of sources but have not been identified before. From these results, this gene can be confirmed at 3D level to encode the iron-sulfur cluster assembly protein IscS and subsequently play a sulfur traffic role. Furthermore, the substrate cysteine can be presumed to be effectively recruited into the active site. Finally, the above detected key residues can be conjectured to be directly responsible for the bind and/or catalysis of PLP and cysteine.展开更多
地铁综合监控系统(ISCS)是保障轨道交通高效安全运营的核心平台,但其安装调试面临多系统接口兼容、分布式网络部署、模型数据合并、联动逻辑验证及冗余机制验证等技术难点。本文针对这些痛点,提出标准化接口适配体系、分层冗余网络部署...地铁综合监控系统(ISCS)是保障轨道交通高效安全运营的核心平台,但其安装调试面临多系统接口兼容、分布式网络部署、模型数据合并、联动逻辑验证及冗余机制验证等技术难点。本文针对这些痛点,提出标准化接口适配体系、分层冗余网络部署、基于 ID 的模型数据合并、场景化联动验证及全场景冗余测试等解决方案。工程实践表明,这些方案可有效提升调试效率,降低故障率,确保 ISCS 实现跨系统集中监控与联动控制,为地铁运营提供可靠技术支撑。展开更多
随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理...随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理,为各类应用场景提供强有力的技术支撑。全面综述了通感算在6G中的应用及其关键技术,探讨了通感算在低空经济、移动通信系统、智能交通系统、工业互联网与智能制造、智慧城市与环境监测等领域中的广泛应用,并深入讨论了通感算融合技术的核心技术,包括通信感知融合(integrated sensing and communication,ISC)技术、通信计算融合(integrated communication and computation,ICC)技术、感知计算融合(integrated sensing and computation,ISAC)技术、通感算融合(integrated sensing,communication,and computation,ISCC)技术及通感算智融合(integrated sensing,communication,computation and intelligence,ISCCI)技术的最新进展。展望了通感算一体化网络在6G时代的发展趋势,重点分析了未来面临的挑战与研究方向。展开更多
基金Project(2004CB619201) supported by the National Basic Research Program of China Project(50321402) supported by the National Natural Science Foundation of China
文摘The gene iscS-3 from ,4cidithiobacillus ferrooxidans may play a central role in the delivery of sulfur to a variety of metabolic pathways in this organism. For insight into the sulfur metabolic mechanism of the bacteria, an integral three-dimensional （3D） molecular structure of the protein encoded by this gene was built by homology modeling techniques, refined by molecular dynamics simulations, assessed by PROFILE-3D and PROSTAT programs and further used to search bind sites, carry out flexible docking with cofactor pyridoxal 5＇-phosphate（PLP） and substrate cysteine and hereby detect its key residues. Through these procedures, the detail conformations of PLP-IscS（P-I） and cysteine-PLP-IscS（C-P-I） complexes were obtained. In P-I complex, the residues of Lys208, His106, Thr78, Ser205, His207, Asp182 and Gln185 have large interaction energies and/or hydrogen bonds fixation with PLP. In C-P-I complex, the amino group in cysteine is very near His106, Lys208 and PLP, the interaction energies for cysteine with them are very high. The above results are well consistent with those experimental facts of the homologues from other sources. Interestingly, the four residues of Glul05, Glu79, Ser203 and Hisl80 in P-I docking and the residue of Lys213 in C-P-I docking also have great interaction energies, which are fitly conservation in IscSs from all kinds of sources but have not been identified before. From these results, this gene can be confirmed at 3D level to encode the iron-sulfur cluster assembly protein IscS and subsequently play a sulfur traffic role. Furthermore, the substrate cysteine can be presumed to be effectively recruited into the active site. Finally, the above detected key residues can be conjectured to be directly responsible for the bind and/or catalysis of PLP and cysteine.
文摘地铁综合监控系统(ISCS)是保障轨道交通高效安全运营的核心平台,但其安装调试面临多系统接口兼容、分布式网络部署、模型数据合并、联动逻辑验证及冗余机制验证等技术难点。本文针对这些痛点,提出标准化接口适配体系、分层冗余网络部署、基于 ID 的模型数据合并、场景化联动验证及全场景冗余测试等解决方案。工程实践表明,这些方案可有效提升调试效率,降低故障率,确保 ISCS 实现跨系统集中监控与联动控制,为地铁运营提供可靠技术支撑。
文摘随着第六代移动通信网络(6th generation mobile network,6G)的不断发展,通感算一体化技术已成为提升未来网络性能和智能化水平的关键技术之一。通感算一体化网络将通信、感知和计算能力深度融合,实现了对信息的全方位获取、传输和处理,为各类应用场景提供强有力的技术支撑。全面综述了通感算在6G中的应用及其关键技术,探讨了通感算在低空经济、移动通信系统、智能交通系统、工业互联网与智能制造、智慧城市与环境监测等领域中的广泛应用,并深入讨论了通感算融合技术的核心技术,包括通信感知融合(integrated sensing and communication,ISC)技术、通信计算融合(integrated communication and computation,ICC)技术、感知计算融合(integrated sensing and computation,ISAC)技术、通感算融合(integrated sensing,communication,and computation,ISCC)技术及通感算智融合(integrated sensing,communication,computation and intelligence,ISCCI)技术的最新进展。展望了通感算一体化网络在6G时代的发展趋势,重点分析了未来面临的挑战与研究方向。
