Interferon regulatory factor 1(IRF-1)is a member of the IRF family.It is the first transcription factor to be identified that could bind to the interferon-stimulated response element(ISRE)on the target gene and displa...Interferon regulatory factor 1(IRF-1)is a member of the IRF family.It is the first transcription factor to be identified that could bind to the interferon-stimulated response element(ISRE)on the target gene and displays crucial roles in the interferoninduced signals and pathways.IRF-1,as an important medium,has all of the advantages of full cell cycle regulation,cell death signaling transduction,and reinforcing immune surveillance,which are well documented.Current studies indicate that IRF-1 is of vital importance to the occurrence and evolution of multifarious liver diseases,including but not limited to inhibiting the replication of the hepatitis virus(A/B/C/E),alleviating the progression of liver fibrosis,and aggravating hepatic ischemiareperfusion injury(HIRI).The tumor suppression of IRF-1 is related to the clinical characteristics of liver cancer patients,which makes it a potential indicator for predicting the prognosis and recurrence of liver cancer;additionally,the latest studies have revealed other effects of IRF-1 such as protection against alcoholic/non-alcoholic fatty liver disease(AFLD/NAFLD),cholangiocarcinoma suppression,and uncommon traits in other liver diseases that had previously received little attention.Intriguingly,several compounds and drugs have featured a protective function in specific liver disease models in which there is significant involvement of the IRF-1 signal.In this paper,we hope to propose a prospective research basis upon which to help decipher translational medicine applications of IRF-1 in liver disease treatment.展开更多
报道了短尾蝮蛇Gloydius brevicaudus干扰素调节因子2基因(IRF-2)的克隆和cDNA全序列测定分析。目前除在爬行类外,IRF-2基因在大部分的脊椎动物如鱼类、两栖类、鸟类和哺乳类都有报道。为了获得爬行类IRF-2基因的全序列,从短尾蝮蛇的肾...报道了短尾蝮蛇Gloydius brevicaudus干扰素调节因子2基因(IRF-2)的克隆和cDNA全序列测定分析。目前除在爬行类外,IRF-2基因在大部分的脊椎动物如鱼类、两栖类、鸟类和哺乳类都有报道。为了获得爬行类IRF-2基因的全序列,从短尾蝮蛇的肾、脾、肝和肠4种组织中使用Trizol试剂盒提取了总RNA。从已知的IRF-2基因序列比对设计了简并引物来扩增保守片段。最后使用RACE方法得到IRF-2的cDNA全序列。结果显示短尾蝮蛇的IRF-2基因开放阅读框包含有978个核苷酸,编码326个氨基酸,其3′UTR包含137个核苷酸。与脊椎动物其他四足动物序列比对分析还发现,短尾蝮蛇的IRF-2基因和推导的氨基酸序列都非常保守,与大部分四足动物的IRF-2相似。从其氨基酸序列中可以辨别出DBD、IDA2和transactivating dom ain等大部分元件和阻遏模体。展开更多
基金supported by the Tianjin Natural Science Foundation(No.19JCZDJC36000)the National Natural Science Foundation of China(No.82241219)+1 种基金the National Major Scientific Research Instrument Development Project of China(No.82127808)the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(No.81921004).
文摘Interferon regulatory factor 1(IRF-1)is a member of the IRF family.It is the first transcription factor to be identified that could bind to the interferon-stimulated response element(ISRE)on the target gene and displays crucial roles in the interferoninduced signals and pathways.IRF-1,as an important medium,has all of the advantages of full cell cycle regulation,cell death signaling transduction,and reinforcing immune surveillance,which are well documented.Current studies indicate that IRF-1 is of vital importance to the occurrence and evolution of multifarious liver diseases,including but not limited to inhibiting the replication of the hepatitis virus(A/B/C/E),alleviating the progression of liver fibrosis,and aggravating hepatic ischemiareperfusion injury(HIRI).The tumor suppression of IRF-1 is related to the clinical characteristics of liver cancer patients,which makes it a potential indicator for predicting the prognosis and recurrence of liver cancer;additionally,the latest studies have revealed other effects of IRF-1 such as protection against alcoholic/non-alcoholic fatty liver disease(AFLD/NAFLD),cholangiocarcinoma suppression,and uncommon traits in other liver diseases that had previously received little attention.Intriguingly,several compounds and drugs have featured a protective function in specific liver disease models in which there is significant involvement of the IRF-1 signal.In this paper,we hope to propose a prospective research basis upon which to help decipher translational medicine applications of IRF-1 in liver disease treatment.
文摘报道了短尾蝮蛇Gloydius brevicaudus干扰素调节因子2基因(IRF-2)的克隆和cDNA全序列测定分析。目前除在爬行类外,IRF-2基因在大部分的脊椎动物如鱼类、两栖类、鸟类和哺乳类都有报道。为了获得爬行类IRF-2基因的全序列,从短尾蝮蛇的肾、脾、肝和肠4种组织中使用Trizol试剂盒提取了总RNA。从已知的IRF-2基因序列比对设计了简并引物来扩增保守片段。最后使用RACE方法得到IRF-2的cDNA全序列。结果显示短尾蝮蛇的IRF-2基因开放阅读框包含有978个核苷酸,编码326个氨基酸,其3′UTR包含137个核苷酸。与脊椎动物其他四足动物序列比对分析还发现,短尾蝮蛇的IRF-2基因和推导的氨基酸序列都非常保守,与大部分四足动物的IRF-2相似。从其氨基酸序列中可以辨别出DBD、IDA2和transactivating dom ain等大部分元件和阻遏模体。
