Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,...Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,and more importantly how GSVs are translocated to the PM(plasma membrane)under insulin stimulation is still under debate.In the present study,we systematically analyzed the dynamics of a large number of single GLUT4-containing vesicles in 3T3-L1 adipocytes by TIRFM(total internal reflection fluorescence microscopy).We found that GLUT4-containing vesicles can be classified into three groups according to their mobility,namely vertical,stable,and lateral GLUT4-containing vesicles.Among these groups,vertical GLUT4-containing vesicles exclude transferrin receptors and move towards the PM specifically in response to insulin stimulation,while stable and lateral GLUT4-containing vesicles contain transferrin receptors and show no insulin responsiveness.These data demonstrate that vertical GLUT4-containing vesicles correspond to specialized secretory GSVs,which approach the PM directly and bypass the constitutive recycling pathway.展开更多
基金Supported by the National Natural Science Foundation of China(Grant Nos.30470448 and 30130230)the National key Basic Research Program of China(Grant No.2004CB720000)+1 种基金the Knowledge Innovative Program of The Chinese Academy of Sciences(Grant Nos.KSCX2-SW-224 and Y2004018)the Li Foundation and the Sinogerman Scientific Center
文摘Insulin-responsive GLUT4(glucose transporter 4)translocation plays a major role in regulating glucose uptake in adipose tissue and muscle.Whether or not there is a specialized secretory GSV(GLUT4 storage vesicle)pool,and more importantly how GSVs are translocated to the PM(plasma membrane)under insulin stimulation is still under debate.In the present study,we systematically analyzed the dynamics of a large number of single GLUT4-containing vesicles in 3T3-L1 adipocytes by TIRFM(total internal reflection fluorescence microscopy).We found that GLUT4-containing vesicles can be classified into three groups according to their mobility,namely vertical,stable,and lateral GLUT4-containing vesicles.Among these groups,vertical GLUT4-containing vesicles exclude transferrin receptors and move towards the PM specifically in response to insulin stimulation,while stable and lateral GLUT4-containing vesicles contain transferrin receptors and show no insulin responsiveness.These data demonstrate that vertical GLUT4-containing vesicles correspond to specialized secretory GSVs,which approach the PM directly and bypass the constitutive recycling pathway.
