Objective:To investigate the regulatory effect of miR-146a overexpression on corneal inflammatory response in a mouse model of dry eye,and to analyze its relationship with the IRAK1/TRAF6/NF-кB signaling pathway.Meth...Objective:To investigate the regulatory effect of miR-146a overexpression on corneal inflammatory response in a mouse model of dry eye,and to analyze its relationship with the IRAK1/TRAF6/NF-кB signaling pathway.Methods:A total of 50 SPF-grade BALB/c mice were randomly divided into five groups,with10 mice in each group.Except for the control group,the other four groups were treated with 0.2%benzalkonium chloride(BAC)solution in both eyes to construct a dry eye model.After successful modeling,the control group and model group received NC agomir;the miR antagonist group received miR-146a antagomir;the miR agonist group received miR-146a agomir;and the pathway agonist group received miR-146a agomir+NF-κB activator 2.After four weeks of treatment,the expressions levels of miR-146a,inflammatory factors,and IRAK1/TRAF6/NF-κB signaling pathway proteins were observed and compared among the five groups.Results:After four weeks of treatment,there was a statistically significant difference in the relative expression of miR-146a in the five groups(F=61.058,P<0.001),which was significantly higher in the miR agonist group than in the other four groups.After4 weeks of treatment,there were statistically significant differences in the expression levels of IL-1β,IL-6,IL-8 and TNF-αin the five groups(F=84.757,103.658,55.477,46.762;P<0.001).After four weeks of treatment,there were statistically significant differences in the protein expression levels of IRAK1,TRAF6,NF-κB and IκBαin the five groups(F=62.975,77.173,67.108,29.381;P<0.001),except for the control group,the expression levels of IRAK1,TRAF6 and NF-κB proteins in the miR agonist group were significantly lower than those in the other three groups,and the expression levels of IκBαprotein were significantly higher than those in the other three groups.Conclusion:Overexpression of miR-146a can negatively regulate corneal inflammatory response in dry eye mice through the IRAK1/TRAF6/NF-κB signaling pathway,which can provide new insights for the clinical treatment of dry eye disease.展开更多
Background Coronary microembolization （CME） is characterized by distal microvascular occlusion. However, the inflammatory mechanisms and therapeutic targets of CME are largely unknown. Methods A total of 11 Guangxi ...Background Coronary microembolization （CME） is characterized by distal microvascular occlusion. However, the inflammatory mechanisms and therapeutic targets of CME are largely unknown. Methods A total of 11 Guangxi Bama miniature swines were divided into two groups： sham （n = 5） and CME （n = 6）. Microspheres were injected into the left anterior descending artery of the CME group to make an animal model of CME. The expres- sions of microRNA-146a （miR-146a） and IRAK1, TRAF6, and AUF1 in the myocardium were detected by qPCR. Results In the CME group, microspheres, microinfarction, and inflammatory cell infiltration were found under an optical microscope. The expression levels of miR-146a were low in both groups. After CME, the expression levels of IRAK1, TRAF6, and AUF1 in the CME group were upregulated compared with those in the sham group （P 〈 0.01;P 〈 0.05;P 〈 0.05, respectively）. Conclusions AUF1, IRAK1 and TRAF6, but not miR-146a, could be involved, in myocardium inflammation following CME.展开更多
本研究检测弥漫大B细胞性淋巴瘤(DLBCL)患者外周血单个核细胞中TRAF6、TAK1及TGF-β基因在化疗前后的表达变化,探讨化疗对TRAF6/TAK1信号通路活性的影响。采用Ct值比较法,通过SYBR Green II实时定量PCR检测38例DLBCL患者化疗前及化疗2...本研究检测弥漫大B细胞性淋巴瘤(DLBCL)患者外周血单个核细胞中TRAF6、TAK1及TGF-β基因在化疗前后的表达变化,探讨化疗对TRAF6/TAK1信号通路活性的影响。采用Ct值比较法,通过SYBR Green II实时定量PCR检测38例DLBCL患者化疗前及化疗2周期后外周血单个核细胞(PBMNC)中TRAF6、TAK1及TGF-β在mRNA的表达水平,并以12例健康人PBMNC作为对照。结果表明:DLBCL患者治疗前后PBMNC中TRAF6、TAK1和TGF-βmRNA表达水平均高于正常人。治疗前患者TRAF6及TAK1基因表达水平与正常人相比未发现统计学差异(P>0.05),治疗后这两基因的表达明显增高,差异具有统计学意义(P<0.05);在此同时治疗后与治疗前相比,这两基因的表达水平也具明显统计学差异(P<0.05),且这两基因的表达水平呈明显正相关,而TGF-β基因治疗后较治疗前明显下降,差异具统计学差异(P<0.05)。结论:DLBCL患者化疗后TRAF6/TAK1信号通路的活性明显增高,而TGF-β基因治疗后则较治疗前明显下降。展开更多
文摘Objective:To investigate the regulatory effect of miR-146a overexpression on corneal inflammatory response in a mouse model of dry eye,and to analyze its relationship with the IRAK1/TRAF6/NF-кB signaling pathway.Methods:A total of 50 SPF-grade BALB/c mice were randomly divided into five groups,with10 mice in each group.Except for the control group,the other four groups were treated with 0.2%benzalkonium chloride(BAC)solution in both eyes to construct a dry eye model.After successful modeling,the control group and model group received NC agomir;the miR antagonist group received miR-146a antagomir;the miR agonist group received miR-146a agomir;and the pathway agonist group received miR-146a agomir+NF-κB activator 2.After four weeks of treatment,the expressions levels of miR-146a,inflammatory factors,and IRAK1/TRAF6/NF-κB signaling pathway proteins were observed and compared among the five groups.Results:After four weeks of treatment,there was a statistically significant difference in the relative expression of miR-146a in the five groups(F=61.058,P<0.001),which was significantly higher in the miR agonist group than in the other four groups.After4 weeks of treatment,there were statistically significant differences in the expression levels of IL-1β,IL-6,IL-8 and TNF-αin the five groups(F=84.757,103.658,55.477,46.762;P<0.001).After four weeks of treatment,there were statistically significant differences in the protein expression levels of IRAK1,TRAF6,NF-κB and IκBαin the five groups(F=62.975,77.173,67.108,29.381;P<0.001),except for the control group,the expression levels of IRAK1,TRAF6 and NF-κB proteins in the miR agonist group were significantly lower than those in the other three groups,and the expression levels of IκBαprotein were significantly higher than those in the other three groups.Conclusion:Overexpression of miR-146a can negatively regulate corneal inflammatory response in dry eye mice through the IRAK1/TRAF6/NF-κB signaling pathway,which can provide new insights for the clinical treatment of dry eye disease.
基金supported by Guangxi Province Medical Technology Research and Development Project(Grant No.S201303_01)Youth Science Foundation of Guangxi Medical University(No.GXMUYSF201213)
文摘Background Coronary microembolization （CME） is characterized by distal microvascular occlusion. However, the inflammatory mechanisms and therapeutic targets of CME are largely unknown. Methods A total of 11 Guangxi Bama miniature swines were divided into two groups： sham （n = 5） and CME （n = 6）. Microspheres were injected into the left anterior descending artery of the CME group to make an animal model of CME. The expres- sions of microRNA-146a （miR-146a） and IRAK1, TRAF6, and AUF1 in the myocardium were detected by qPCR. Results In the CME group, microspheres, microinfarction, and inflammatory cell infiltration were found under an optical microscope. The expression levels of miR-146a were low in both groups. After CME, the expression levels of IRAK1, TRAF6, and AUF1 in the CME group were upregulated compared with those in the sham group （P 〈 0.01;P 〈 0.05;P 〈 0.05, respectively）. Conclusions AUF1, IRAK1 and TRAF6, but not miR-146a, could be involved, in myocardium inflammation following CME.
文摘本研究检测弥漫大B细胞性淋巴瘤(DLBCL)患者外周血单个核细胞中TRAF6、TAK1及TGF-β基因在化疗前后的表达变化,探讨化疗对TRAF6/TAK1信号通路活性的影响。采用Ct值比较法,通过SYBR Green II实时定量PCR检测38例DLBCL患者化疗前及化疗2周期后外周血单个核细胞(PBMNC)中TRAF6、TAK1及TGF-β在mRNA的表达水平,并以12例健康人PBMNC作为对照。结果表明:DLBCL患者治疗前后PBMNC中TRAF6、TAK1和TGF-βmRNA表达水平均高于正常人。治疗前患者TRAF6及TAK1基因表达水平与正常人相比未发现统计学差异(P>0.05),治疗后这两基因的表达明显增高,差异具有统计学意义(P<0.05);在此同时治疗后与治疗前相比,这两基因的表达水平也具明显统计学差异(P<0.05),且这两基因的表达水平呈明显正相关,而TGF-β基因治疗后较治疗前明显下降,差异具统计学差异(P<0.05)。结论:DLBCL患者化疗后TRAF6/TAK1信号通路的活性明显增高,而TGF-β基因治疗后则较治疗前明显下降。
