This study analyzed and predicted following aspects of isopentenyl py- rophosphate isomerases （IPIs） of five north medicinal plants using bioinformatics methods and tools： physical and chemical properties, hydropho...This study analyzed and predicted following aspects of isopentenyl py- rophosphate isomerases （IPIs） of five north medicinal plants using bioinformatics methods and tools： physical and chemical properties, hydrophobicity/hydrophilicity, trans-membrane domain, secondary structure, subcellular localization and so on. The results showed that： there was no notable difference among the physical and chem- ical properties of IPIs of the five north medicinal plants; the IPIs were mainly hy- drophilic; the IPIs were mainly located in chloroplasts by subcellular localization; serine phosphorylation sites were the most; the secondary structures mainly consist- ed of c^-helixes and random coils; no signal peptide existed, indicating that the pro- tein IPI was non-secreted protein; no trans-membrane domain existed; and one functional domain was shown, Le., Nudix Hydrolase Superfamily. This study is of great significance to research on IPI gene functions, deep research on north medic- inal plants, improvement of efficacy of north medicinal plants and rational develop- ment and utilization of medicinal plant resources.展开更多
目的探讨外周血miR-141、miR-451a与弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者化疗应答的预测价值。方法选取2021年6月至2023年4月我院92例DLBCL患者作为DLBCL组,根据化疗效果分为化疗无效亚组(n=29)与化疗有效亚组(...目的探讨外周血miR-141、miR-451a与弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者化疗应答的预测价值。方法选取2021年6月至2023年4月我院92例DLBCL患者作为DLBCL组,根据化疗效果分为化疗无效亚组(n=29)与化疗有效亚组(n=63)。随机选取同期92例入院体检健康者为对照组,采用实时荧光定量聚合酶链反应测定miR-141、miR-451a相对表达量。比较DLBCL组与健康对照组外周血miR-141、miR-451a表达,以logistic回归模型分析筛选DLBCL患者化疗应答影响因素,相关性分析DLBCL患者外周血miR-141、miR-451a与国际预后指数(international prognositic index,IPI)评分、Ann Arbor分期间相关性,受试者工作特征(receiver operating characteristic,ROC)曲线评价DLBCL患者miR-141、miR-451a单项检测及联合检测预测化疗应答的价值。结果DLBCL患者外周血miR-141、miR-451a表达均低于健康对照组(P<0.05);logistic回归分析结果显示Ann Arbor分期、IPI评分均为DLBCL患者化疗应答独立危险因素,外周血miR-141、miR-451a均为DLBCL患者化疗应答性独立保护因素(P<0.05);DLBCL患者外周血miR-141、miR-451a与IPI评分、Ann Arbor分期均具有显著负相关关系(P<0.05);外周血miR-141、miR-451a单独预测DLBCL患者化疗应答的曲线下面积(area under the curve,AUC)值分别为0.770、0.794,二者联合预测AUC值高达0.929,明显高于miR-141、miR-451a单独预测,此时灵敏度、特异度分别为86.21%、85.71%。结论DCBCL患者血清miR-141、miR-451a表达下调,且与应答有关,检测二者水平,可预测DCBCL患者化疗应答,为临床工作提供参考。展开更多
基金Supported by Science and Technology Planning Project of Mudanjiang(G2015d1974)Funding Project of Training of Famous Teachers in Mudanjiang Normal University(2014QNGG1805)~~
文摘This study analyzed and predicted following aspects of isopentenyl py- rophosphate isomerases （IPIs） of five north medicinal plants using bioinformatics methods and tools： physical and chemical properties, hydrophobicity/hydrophilicity, trans-membrane domain, secondary structure, subcellular localization and so on. The results showed that： there was no notable difference among the physical and chem- ical properties of IPIs of the five north medicinal plants; the IPIs were mainly hy- drophilic; the IPIs were mainly located in chloroplasts by subcellular localization; serine phosphorylation sites were the most; the secondary structures mainly consist- ed of c^-helixes and random coils; no signal peptide existed, indicating that the pro- tein IPI was non-secreted protein; no trans-membrane domain existed; and one functional domain was shown, Le., Nudix Hydrolase Superfamily. This study is of great significance to research on IPI gene functions, deep research on north medic- inal plants, improvement of efficacy of north medicinal plants and rational develop- ment and utilization of medicinal plant resources.
文摘目的探讨外周血miR-141、miR-451a与弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者化疗应答的预测价值。方法选取2021年6月至2023年4月我院92例DLBCL患者作为DLBCL组,根据化疗效果分为化疗无效亚组(n=29)与化疗有效亚组(n=63)。随机选取同期92例入院体检健康者为对照组,采用实时荧光定量聚合酶链反应测定miR-141、miR-451a相对表达量。比较DLBCL组与健康对照组外周血miR-141、miR-451a表达,以logistic回归模型分析筛选DLBCL患者化疗应答影响因素,相关性分析DLBCL患者外周血miR-141、miR-451a与国际预后指数(international prognositic index,IPI)评分、Ann Arbor分期间相关性,受试者工作特征(receiver operating characteristic,ROC)曲线评价DLBCL患者miR-141、miR-451a单项检测及联合检测预测化疗应答的价值。结果DLBCL患者外周血miR-141、miR-451a表达均低于健康对照组(P<0.05);logistic回归分析结果显示Ann Arbor分期、IPI评分均为DLBCL患者化疗应答独立危险因素,外周血miR-141、miR-451a均为DLBCL患者化疗应答性独立保护因素(P<0.05);DLBCL患者外周血miR-141、miR-451a与IPI评分、Ann Arbor分期均具有显著负相关关系(P<0.05);外周血miR-141、miR-451a单独预测DLBCL患者化疗应答的曲线下面积(area under the curve,AUC)值分别为0.770、0.794,二者联合预测AUC值高达0.929,明显高于miR-141、miR-451a单独预测,此时灵敏度、特异度分别为86.21%、85.71%。结论DCBCL患者血清miR-141、miR-451a表达下调,且与应答有关,检测二者水平,可预测DCBCL患者化疗应答,为临床工作提供参考。
