H-FABP(Heart fatty acid-binding protein),a member of FABP family,plays an essential role in long-chain fatty acid uptake and metabolic homeostasis.Its role in pig intramuscular fat content remains poorly understood,es...H-FABP(Heart fatty acid-binding protein),a member of FABP family,plays an essential role in long-chain fatty acid uptake and metabolic homeostasis.Its role in pig intramuscular fat content remains poorly understood,especially in local pig breeds in western China.In this study,the genetic variations of 5'-upstream region and the second intron in porcine H-FABP gene were investigated by PCR-RFLP in 256 pigs including Duroc,Large White,Landrace,Neijiang,Rongchang,Bamei pig,Hanjiang Black,Hanzhong White,and the wild ones.The effect of H-FABP gene on the IMF content was analyzed by the least square method.Lipid droplet morphology and content in adipocytes cultured from pigs with different H-FABP genotypes,were studied by oil red O staining and a triglyceride assay kit.Results showed a HinfⅠ-RFLP in these eight pig breeds and wild pigs,among which Large white,Bamei pig,Hanjiang Black,Hanzhong White,and wild pigs presented with low polymorphism while the other breeds had intermediate polymorphism.There was no HaeⅢor MspⅠ-RFLPs in the four Chinese local pig breeds tested,but Duroc,Landrace,Large white,Hanzhong White and wild pig had polymorphism.Landrace,Large White and wild pigs had low levels of HaeⅢand MspⅠ-RFLP,whereas others had intermediate polymorphism.H-FABP genotypes significantly affected the IMF content(P<0.05).The IMF content ordered by H-FABP genotypes were HH>Hh>hh,DD<Dd<dd,and AA<Aa<aa.The genetic effect values were 3.89,3.42,3.17,2.27,2.49,2.91,2.28,2.70,and 2.95,respectively.Fat deposition in adipocytes was stronger in the HH,dd and aa genotypes than in others.The results suggest that porcine meat quality may be improved by increasing the frequency of genotype aa-dd-HH in pig breeds.展开更多
为了揭示脂肪型的藏猪和瘦肉型的杜洛克猪胰岛素样生长因子2(IGF-2)基因的表达差异,采用QRT-PCR方法检测了两猪种背最长肌中IGF-2基因在180日龄的表达差异,并分析其与肌纤维面积、肌内脂肪(IMF)含量的相关性。结果表明,180日龄藏猪IGF-2...为了揭示脂肪型的藏猪和瘦肉型的杜洛克猪胰岛素样生长因子2(IGF-2)基因的表达差异,采用QRT-PCR方法检测了两猪种背最长肌中IGF-2基因在180日龄的表达差异,并分析其与肌纤维面积、肌内脂肪(IMF)含量的相关性。结果表明,180日龄藏猪IGF-2 m RNA表达量显著低于杜洛克猪(P<0.05)。相关性分析结果显示,IGF-2 m RNA表达量与肌纤维面积呈显著正相关(P<0.05),与IMF含量呈显著负相关(P<0.05)。以上结果初步揭示了两猪种在180日龄IGF-2基因表达的品种差异,为深入研究肌纤维生长及IMF沉积的调控机制提供了基础数据。展开更多
基金This work was supported by the Special Funds for Major State Basic Research of China(No.2004CB117506)National Nature Sciences Foundation of China(No.30471239)
文摘H-FABP(Heart fatty acid-binding protein),a member of FABP family,plays an essential role in long-chain fatty acid uptake and metabolic homeostasis.Its role in pig intramuscular fat content remains poorly understood,especially in local pig breeds in western China.In this study,the genetic variations of 5'-upstream region and the second intron in porcine H-FABP gene were investigated by PCR-RFLP in 256 pigs including Duroc,Large White,Landrace,Neijiang,Rongchang,Bamei pig,Hanjiang Black,Hanzhong White,and the wild ones.The effect of H-FABP gene on the IMF content was analyzed by the least square method.Lipid droplet morphology and content in adipocytes cultured from pigs with different H-FABP genotypes,were studied by oil red O staining and a triglyceride assay kit.Results showed a HinfⅠ-RFLP in these eight pig breeds and wild pigs,among which Large white,Bamei pig,Hanjiang Black,Hanzhong White,and wild pigs presented with low polymorphism while the other breeds had intermediate polymorphism.There was no HaeⅢor MspⅠ-RFLPs in the four Chinese local pig breeds tested,but Duroc,Landrace,Large white,Hanzhong White and wild pig had polymorphism.Landrace,Large White and wild pigs had low levels of HaeⅢand MspⅠ-RFLP,whereas others had intermediate polymorphism.H-FABP genotypes significantly affected the IMF content(P<0.05).The IMF content ordered by H-FABP genotypes were HH>Hh>hh,DD<Dd<dd,and AA<Aa<aa.The genetic effect values were 3.89,3.42,3.17,2.27,2.49,2.91,2.28,2.70,and 2.95,respectively.Fat deposition in adipocytes was stronger in the HH,dd and aa genotypes than in others.The results suggest that porcine meat quality may be improved by increasing the frequency of genotype aa-dd-HH in pig breeds.
文摘为了揭示脂肪型的藏猪和瘦肉型的杜洛克猪胰岛素样生长因子2(IGF-2)基因的表达差异,采用QRT-PCR方法检测了两猪种背最长肌中IGF-2基因在180日龄的表达差异,并分析其与肌纤维面积、肌内脂肪(IMF)含量的相关性。结果表明,180日龄藏猪IGF-2 m RNA表达量显著低于杜洛克猪(P<0.05)。相关性分析结果显示,IGF-2 m RNA表达量与肌纤维面积呈显著正相关(P<0.05),与IMF含量呈显著负相关(P<0.05)。以上结果初步揭示了两猪种在180日龄IGF-2基因表达的品种差异,为深入研究肌纤维生长及IMF沉积的调控机制提供了基础数据。
