目的:评估基于马斯洛理论联合家庭支持的IMB护理模型对急性脑梗死(ACI)患者居家护理干预的效果。本研究方法分为对照组、马斯洛理论组、IMB模型组和综合组。对照组接受常规护理,综合组接受基于马斯洛理论联合家庭支持的IMB护理模型,其...目的:评估基于马斯洛理论联合家庭支持的IMB护理模型对急性脑梗死(ACI)患者居家护理干预的效果。本研究方法分为对照组、马斯洛理论组、IMB模型组和综合组。对照组接受常规护理,综合组接受基于马斯洛理论联合家庭支持的IMB护理模型,其他两组仅接受一种护理模型。结果:综合组的护理模型可以降低ACI后患者的复发率和发生并发症的可能性,改善患者的术后生活质量。结论:基于马斯洛理论联合家庭支持的IMB护理模型可提高ACI患者居家护理的效果。Objective: To evaluate the effect of Maslow theory combined with family support IMB nursing model on home nursing intervention for patients with acute cerebral infarction (ACI). Methods: The study was divided into control group, Maslow theory group, IMB model group and comprehensive group. The control group received routine care, the comprehensive group received Maslow’s theory combined with family support IMB care model, and the other two groups received only one care model. Results The nursing mode of comprehensive group could reduce the recurrence rate and the possibility of complications after ACI, and improve the quality of life of patients after ACI. Conclusion: IMB nursing model based on Maslow theory combined with family support can improve the effect of home nursing for ACI patients.展开更多
[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chroma...[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chromatography.[Method]Feed samples were extracted using ultra-pure water,purified by automatic IMBs,and subsequently analyzed via high-performance liquid chromatography,employing an external standard method for quantification.[Result]A satisfactory linearity was achieved for DON within the concentration range of 0.05 to 2.0μg/mL,with the corresponding correlation coefficients(R^(2))exceeding 0.9999.The limit of detection(LOD)and limit of quantification(LOQ)for the proposed method were determined to be 0.03 and 0.1 mg/kg,respectively.The average recoveries of the fortified samples(0.1,0.2 and 1.0 mg/kg)were 88.5%−100.6%,with the relative standard deviations(RSD)ranging from 2.1%to 9.7%.[Conclusion]In comparison with the traditional solidphase extraction and immunoaffinity column purification methods,the IMBs technique consolidates the extraction,separation,and purification into a single process.This approach enables fully automated processing,which significantly enhances work efficiency and mitigates result deviations that may arise from manual operations.Consequently,this technique is particularly well-suited for the determination of DON in a large number of feed samples.展开更多
Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management pro...Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.展开更多
目的:观察信息-动机-行为(information-motivation-behavioral,IMB)理论下护理对结直肠癌造口患者术后恢复和自我效能感的影响。方法:选取2022年10月—2024年10月山东大学齐鲁医院收治的92例结直肠癌造口患者,按照随机信封法分为观察组...目的:观察信息-动机-行为(information-motivation-behavioral,IMB)理论下护理对结直肠癌造口患者术后恢复和自我效能感的影响。方法:选取2022年10月—2024年10月山东大学齐鲁医院收治的92例结直肠癌造口患者,按照随机信封法分为观察组与对照组,各46例。对照组采用常规术后护理,观察组在对照组基础上联用IMB理论下护理,比较两组造口情况、自我效能、术后并发症、疾病不确定感、负性情绪及术后体征恢复情况。结果:护理结束后,观察组造口生活质量量表(city of hope-quality of life-ostomy questionnaire-Chinese version,C-COH)评分高于对照组,造口周围皮肤状况量表(DET score of skin health around stoma,DET)评分低于对照组,差异有统计学意义(P<0.05);护理结束后,两组一般自我效能量表(general self-efficacy scale,GSES)评分高于护理前,疾病不确定感量表(Mishel uncertainty in illness scale,MUIS-A)、焦虑自评量表(self-rating anxiety scale,SAS)、汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评分低于护理前,且观察组GSES评分高于对照组,MUIS-A评分、SAS评分、HAMD评分低于对照组,差异有统计学意义(P<0.05);观察组术后并发症发生率低于对照组,术后肠鸣音恢复时间、首次排气时间、住院时间短于对照组,差异有统计学意义(P<0.05)。结论:IMB理论下护理在结直肠癌造口患者术后恢复中的应用效果较好,能够促进生活质量提升,增强自我效能感,减少术后并发症发生,并消除疾病不确定感,改善负性情绪。展开更多
文摘目的:评估基于马斯洛理论联合家庭支持的IMB护理模型对急性脑梗死(ACI)患者居家护理干预的效果。本研究方法分为对照组、马斯洛理论组、IMB模型组和综合组。对照组接受常规护理,综合组接受基于马斯洛理论联合家庭支持的IMB护理模型,其他两组仅接受一种护理模型。结果:综合组的护理模型可以降低ACI后患者的复发率和发生并发症的可能性,改善患者的术后生活质量。结论:基于马斯洛理论联合家庭支持的IMB护理模型可提高ACI患者居家护理的效果。Objective: To evaluate the effect of Maslow theory combined with family support IMB nursing model on home nursing intervention for patients with acute cerebral infarction (ACI). Methods: The study was divided into control group, Maslow theory group, IMB model group and comprehensive group. The control group received routine care, the comprehensive group received Maslow’s theory combined with family support IMB care model, and the other two groups received only one care model. Results The nursing mode of comprehensive group could reduce the recurrence rate and the possibility of complications after ACI, and improve the quality of life of patients after ACI. Conclusion: IMB nursing model based on Maslow theory combined with family support can improve the effect of home nursing for ACI patients.
基金Supported by National Key Research and Development Program of China(2023YFD1301001)Central Public-interest Scientific Institution Basal Research Fund(1610072023005)Agricultural Science and Technology Innovation Program of CAAS(CAAS-ASTIP-IQSTAP-04).
文摘[Objective]The aim of this work was to establish an analytical method for the determination of deoxynivalenol(DON)in feeds using automatic immunomagnetic beads(IMBs)clean-up coupled with high-performance liquid chromatography.[Method]Feed samples were extracted using ultra-pure water,purified by automatic IMBs,and subsequently analyzed via high-performance liquid chromatography,employing an external standard method for quantification.[Result]A satisfactory linearity was achieved for DON within the concentration range of 0.05 to 2.0μg/mL,with the corresponding correlation coefficients(R^(2))exceeding 0.9999.The limit of detection(LOD)and limit of quantification(LOQ)for the proposed method were determined to be 0.03 and 0.1 mg/kg,respectively.The average recoveries of the fortified samples(0.1,0.2 and 1.0 mg/kg)were 88.5%−100.6%,with the relative standard deviations(RSD)ranging from 2.1%to 9.7%.[Conclusion]In comparison with the traditional solidphase extraction and immunoaffinity column purification methods,the IMBs technique consolidates the extraction,separation,and purification into a single process.This approach enables fully automated processing,which significantly enhances work efficiency and mitigates result deviations that may arise from manual operations.Consequently,this technique is particularly well-suited for the determination of DON in a large number of feed samples.
基金supported by the National Key Research&Development Program(2024YFF0618103,2023YFF0611503,2023YFF1105102)the Science and Technology Program of State Administration for Market Regulation(2024MK170)+1 种基金the Science and Technology Project of Jiangsu Market Supervision and Administration(KJ2024014,KJ2024059)the Science and Technology Program of Nanjing Market Supervision and Administration(Kj2023006).
文摘Common bunt is a major disease of wheat worldwide that reduces crop yields and grain quality.Rapid and sensitive quantitative detection methods are required to diagnose and monitor this disease in wheat management programs and to ensure seed quality.In this study,an immunomagnetic beads-based enzyme-linked immunosorbent assay(IMBs-ELISA)was developed for the detection of Tilletia foetida teliospores in wheat and flour.An anti-T.foetida teliospores polyclonal antibody bound to immunomagnetic beads was used as the capture probe,and a polyclonal antibody labeled with horseradish peroxidase was used as the detector probe.The capture and detection conditions for the target spores were optimized to achieve the best determination results.Under optimal conditions,the proposed method took less than 2 h to complete.Its limit of detection was 300 teliospores per gram.The critical reaction in this IMBs-ELISA occurred on magnetic beads.This not only simplified the traditional ELISA process,but also shortened the detection time.This study has expanded the application of the IMBs-ELISA method to fungal spore detection.This method has potential applications in agriculture and seed management.
文摘目的:观察信息-动机-行为(information-motivation-behavioral,IMB)理论下护理对结直肠癌造口患者术后恢复和自我效能感的影响。方法:选取2022年10月—2024年10月山东大学齐鲁医院收治的92例结直肠癌造口患者,按照随机信封法分为观察组与对照组,各46例。对照组采用常规术后护理,观察组在对照组基础上联用IMB理论下护理,比较两组造口情况、自我效能、术后并发症、疾病不确定感、负性情绪及术后体征恢复情况。结果:护理结束后,观察组造口生活质量量表(city of hope-quality of life-ostomy questionnaire-Chinese version,C-COH)评分高于对照组,造口周围皮肤状况量表(DET score of skin health around stoma,DET)评分低于对照组,差异有统计学意义(P<0.05);护理结束后,两组一般自我效能量表(general self-efficacy scale,GSES)评分高于护理前,疾病不确定感量表(Mishel uncertainty in illness scale,MUIS-A)、焦虑自评量表(self-rating anxiety scale,SAS)、汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评分低于护理前,且观察组GSES评分高于对照组,MUIS-A评分、SAS评分、HAMD评分低于对照组,差异有统计学意义(P<0.05);观察组术后并发症发生率低于对照组,术后肠鸣音恢复时间、首次排气时间、住院时间短于对照组,差异有统计学意义(P<0.05)。结论:IMB理论下护理在结直肠癌造口患者术后恢复中的应用效果较好,能够促进生活质量提升,增强自我效能感,减少术后并发症发生,并消除疾病不确定感,改善负性情绪。
