Background:Interleukin 13 receptor subunit alpha 2(IL13RA2)plays an essential role in the progression of many cancers.However,the role of IL13RA2 in infantile haemangioma(IH)is still unknown.Materials and Methods:IL13...Background:Interleukin 13 receptor subunit alpha 2(IL13RA2)plays an essential role in the progression of many cancers.However,the role of IL13RA2 in infantile haemangioma(IH)is still unknown.Materials and Methods:IL13RA2 expression in IH tissues was analyzed using western blot,qRT-PCR,and immunofluorescence.The role of IL13RA2 in haemangioma-derived endothelial cells(HemECs)was determined following knockdown or overexpression of IL13RA2 using CCK-8,colony formation,apoptosis,wound healing,tubule formation,Transwell,and western blot.Results:IL13RA2 expression was upregulated in IH tissues.IL13RA2 overexpression promoted proliferation,migration,and invasion of HemECs and induced glycolysis,which was confirmed with a glycolysis inhibitor.Specifically,IL13RA2 interacted withβ-catenin and activated the Wnt/β-catenin pathway in HemECs,which were involved in the above-mentioned effects of IL13RA2.Conclusions:These findings revealed that targeting IL13RA2 is a potential therapeutic approach for IH.展开更多
目的:探讨Toll样受体4(toll-like receptor 4,TLR4)Thr399Ile基因多态性与胃癌易感性的关系。方法:通过计算机检索及手工检索,收集有关TLR4 Thr399Ile基因多态性与胃癌易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对...目的:探讨Toll样受体4(toll-like receptor 4,TLR4)Thr399Ile基因多态性与胃癌易感性的关系。方法:通过计算机检索及手工检索,收集有关TLR4 Thr399Ile基因多态性与胃癌易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对各项研究进行异质性检验,计算合并OR值及其95%CI,并行敏感性分析和发表偏倚的评估。结果:8篇文献纳入本研究,共计有1297例胃癌患者和2702例对照人群。TLR4Thr399Ile基因多态性与胃癌易感性的研究结果为:T versus C:OR=1.307,95%CI=1.047-1.631;TT versus CC:OR=1.527,95%CI=0.398-5.860;TC versus CC:OR=1.324,95%CI=1.049-1.670;TT/TC versus CC:OR=1.326,95%CI=1.053-1.671;TT versus TC/CC:OR=1.481,95%CI=0.386-5.685。根据种族来源进行分层分析,在高加索人群中的研究结果为:T versus C:OR=1.298,95%CI=1.027-1.640;TC versus CC:OR=1.313,95%CI=1.027-1.679;TT/TC versus CC:OR=1.316,95%CI=1.031-1.679。结论:TLR4Thr399Ile TC、TT/TC基因型及T等位基因能增加胃癌的患病风险。展开更多
AIM:To analyze the association of three IL28B single nucleotide polymorphisms with response to therapy in Chilean patients infected with hepatitis C virus CV.METHODS:We studied two groups of patients with chronic CV i...AIM:To analyze the association of three IL28B single nucleotide polymorphisms with response to therapy in Chilean patients infected with hepatitis C virus CV.METHODS:We studied two groups of patients with chronic CV infection genotype 1,under standard combined treatment with pegylated interferon plus ribavirin.One group consisted of 50 patients with sustained virological response,whereas the second group consisted of 49 null responders.In order to analyze the IL28B single nucleotide polymorphisms rs12979860,rs12980275 and rs8099917,samples were used for polymerase chain reaction amplification,and the genotyping was performed by restriction fragment lengthpolymorphism.RESULTS:The IL28B rs12979860 CC,rs12980275 AA and rs8099917 TT genotypes were much more frequently found in patients with sustained virological response compared to null responders 38%,44% and 50% vs 2%,8.2% and 8.2%,respectively.These differences were highly significant in all three cases(P < 0.0001.CONCLUSION:The three IL28B polymorphisms studied are strongly associated with sustained virological response to therapy in Chilean patients with chronic CV genotype 1.展开更多
BACKGROUND Infantile-onset inflammatory bowel disease(IO-IBD)occurs in very young children and causes severe clinical manifestations,which has poor responses to traditional inflammatory bowel disease(IBD)treatments.At...BACKGROUND Infantile-onset inflammatory bowel disease(IO-IBD)occurs in very young children and causes severe clinical manifestations,which has poor responses to traditional inflammatory bowel disease(IBD)treatments.At present,there are no simple and reliable laboratory indicators for early screening IO-IBD patients,especially those in whom the disease is caused by monogenic diseases.AIM To search for valuable indicators for early identifying IO-IBD patients,especially those in whom the disease is caused by monogenic diseases.METHODS A retrospective analysis was performed in 73 patients with IO-IBD admitted to our hospital in the past 5 years.Based on the next-generation sequencing results,they were divided into a monogenic IBD group(M-IBD)and a non-monogenic IBD group(NM-IBD).Forty age-matched patients with allergic proctocolitis(AP)were included in a control group.The clinical manifestations and the inflammatory factors in peripheral blood were evaluated.Logistic regression analysis and receiver operating characteristic(ROC)curve analysis were used to identify the screening factors and cut-off values of IO-IBD as well as monogenic IO-IBD,respectively.RESULTS Among the 44 M-IBD patients,35 carried IL-10RA mutations,and the most common mutations were c.301C>T(p.R101W,30/70)and the c.537G>A(p.T179T,17/70).Patients with higher serum tumor necrosis factor(TNF)-αvalue were more likely to have IBD[odds ratio(OR)=1.25,95%confidence interval(CI):1.05-1.50,P=0.013],while higher serum albumin level was associated with lower risk of IBD(OR=0.86,95%CI:0.74-1.00,P=0.048).The cut-off values of TNF-αand albumin were 17.40 pg/mL(sensitivity:0.78;specificity:0.88)and 36.50 g/L(sensitivity:0.80;specificity:0.90),respectively.The increased ferritin level was indicative of a genetic mutation in IO-IBD patients.Its cut-off value was 28.20 ng/mL(sensitivity:0.93;specificity:0.92).When interleukin(IL)-10 level was higher than 33.05 pg/mL(sensitivity:1.00;specificity:0.84),or the onset age was earlier than 0.21 mo(sensitivity:0.82;specificity:0.94),the presence of diseasecausing mutations in IL-10RA in IO-IBD patients was strongly suggested.CONCLUSION Serum TNF-αand albumin level could differentiate IO-IBD patients from allergic proctocolitis patients,and serum ferritin and IL-10 levels are useful indicators for early diagnosing monogenic IO-IBD.展开更多
The controllable molecular weight and polydispersity of polyacrylonitriles(PANs) were synthesized in ionic liquids(ILs) by atom transfer radical polymerization(ATRP) using ethyl-2 bromopropionate(EPN-Br) as initiator ...The controllable molecular weight and polydispersity of polyacrylonitriles(PANs) were synthesized in ionic liquids(ILs) by atom transfer radical polymerization(ATRP) using ethyl-2 bromopropionate(EPN-Br) as initiator and CuBr/pentamethyldiethylenetria-mine(PMDETA) as catalyst.The polydispersity index(PDI) in the ATRP of acrylonitriel(AN) in ILs is lower than 1.3.From the polymerization kinetics,plots of ln([M]0/[M]) with time show a linear dependence at the early stage of polymerization while the deviation is observed with the conversion rate increasing.The polymerization rate in ILs,especially in 1-butyl-3-methylimidazolium chloride([bmim]Cl),is higher than that in N,N-dimethylformamide(DMF).The polymerization rate increased and the average molecular weight decreased with temperature increasing.The polymerization rate,molecular weight and PDI varied with the variation of [AN]0:[EPN-Br]0:[CuBr]0:[PMDETA]0 ratio in the experimental range,the polymerization of AN in [bmim]Cl displayed living polymerization characteristics.Moreover,the catalyst and ILs are easily isolated from the polymer and reused.展开更多
Background: Genetic variation influencing individual susceptibility to chemical carcinogens is one of the main factors leading to cancer development. The glutathione S-transferases (GSTs) are a family of enzymes belon...Background: Genetic variation influencing individual susceptibility to chemical carcinogens is one of the main factors leading to cancer development. The glutathione S-transferases (GSTs) are a family of enzymes belonging to phase II enzymes involved in detoxification of xenobiotics. A significant relationship is observed between the risk of developing cancer and genetic polymorphisms within GSTs. Methods: In this study, we investigated the influence of inherited GSTP1 (Ile105Val) gene polymorphism on the susceptibility to CML in Egypt in 40 CML patients (20 children and 20 adults), together with 40 healthy controls using a [PCR-RFLP] assay. Results: We found that the mutant type (IIe/Val, Val/Val) was significantly higher in CML patients (67.5%) compared to controls (35%) (p = 0.004);[odds ratio 3.9;95% CI: 1.5 - 9.7]. The mutant type was associated with more advanced phases in disease and with both worse hematological and cytogenetic responses when compared to the wild type (p = 0.03, p = 0.05, and p 0.001, respectively). Conclusion: GSTP1 (Ile105Val) gene polymorphism conferred a significant association with increased risk of CML and is associated with worse prognosis. Further studies on the functional consequences of this genetic polymorphism would pave the way to declare its role in the pathogenesis of CML or as a possible predictor for response to therapy.展开更多
Among patients with familial amyloid polyneuropathy (FAP), those with transth yretin Val30Met mainly show distally predominant weakness and atrophy, whereas s ome FAP patients, including those with transthyretin Ser50...Among patients with familial amyloid polyneuropathy (FAP), those with transth yretin Val30Met mainly show distally predominant weakness and atrophy, whereas s ome FAP patients, including those with transthyretin Ser50Ile and Tyr114Cys, sho w muscle weakness and atrophy that is dominant proximally, simulating myopathy. To clarify the cause of proximally dominant muscular atrophy in patients with FA P transthyretin Ser50Ile and Tyr114Cys, we investigated the distinctive features of muscle specimens of patients with FAP, 3 of who had Val30Met, 2 Ser50Ile, an d 2 Tyr114Cys transthyretin. All specimens showed transthyretin amyloid around b lood vessels and perimysium, and neurogenic denervation patterns. The amount of amyloid around the vessels was much greater in patients with FAP Ser50Ile and Ty r114Cys than in Val30Met patients. Muscular amyloid angiopathy may contribute to motor nerve injury that, in turn, may lead to amyotropic changes in patients wi th FAP Ser50Ile and Tyr114Cys.展开更多
文摘Background:Interleukin 13 receptor subunit alpha 2(IL13RA2)plays an essential role in the progression of many cancers.However,the role of IL13RA2 in infantile haemangioma(IH)is still unknown.Materials and Methods:IL13RA2 expression in IH tissues was analyzed using western blot,qRT-PCR,and immunofluorescence.The role of IL13RA2 in haemangioma-derived endothelial cells(HemECs)was determined following knockdown or overexpression of IL13RA2 using CCK-8,colony formation,apoptosis,wound healing,tubule formation,Transwell,and western blot.Results:IL13RA2 expression was upregulated in IH tissues.IL13RA2 overexpression promoted proliferation,migration,and invasion of HemECs and induced glycolysis,which was confirmed with a glycolysis inhibitor.Specifically,IL13RA2 interacted withβ-catenin and activated the Wnt/β-catenin pathway in HemECs,which were involved in the above-mentioned effects of IL13RA2.Conclusions:These findings revealed that targeting IL13RA2 is a potential therapeutic approach for IH.
文摘目的:探讨Toll样受体4(toll-like receptor 4,TLR4)Thr399Ile基因多态性与胃癌易感性的关系。方法:通过计算机检索及手工检索,收集有关TLR4 Thr399Ile基因多态性与胃癌易感性的文献,筛选出符合纳入和排除标准的文献,应用Meta分析软件对各项研究进行异质性检验,计算合并OR值及其95%CI,并行敏感性分析和发表偏倚的评估。结果:8篇文献纳入本研究,共计有1297例胃癌患者和2702例对照人群。TLR4Thr399Ile基因多态性与胃癌易感性的研究结果为:T versus C:OR=1.307,95%CI=1.047-1.631;TT versus CC:OR=1.527,95%CI=0.398-5.860;TC versus CC:OR=1.324,95%CI=1.049-1.670;TT/TC versus CC:OR=1.326,95%CI=1.053-1.671;TT versus TC/CC:OR=1.481,95%CI=0.386-5.685。根据种族来源进行分层分析,在高加索人群中的研究结果为:T versus C:OR=1.298,95%CI=1.027-1.640;TC versus CC:OR=1.313,95%CI=1.027-1.679;TT/TC versus CC:OR=1.316,95%CI=1.031-1.679。结论:TLR4Thr399Ile TC、TT/TC基因型及T等位基因能增加胃癌的患病风险。
基金Supported by The grant OAIC 394/10(to M.V.)from Hospital Clínico Universidad de Chile
文摘AIM:To analyze the association of three IL28B single nucleotide polymorphisms with response to therapy in Chilean patients infected with hepatitis C virus CV.METHODS:We studied two groups of patients with chronic CV infection genotype 1,under standard combined treatment with pegylated interferon plus ribavirin.One group consisted of 50 patients with sustained virological response,whereas the second group consisted of 49 null responders.In order to analyze the IL28B single nucleotide polymorphisms rs12979860,rs12980275 and rs8099917,samples were used for polymerase chain reaction amplification,and the genotyping was performed by restriction fragment lengthpolymorphism.RESULTS:The IL28B rs12979860 CC,rs12980275 AA and rs8099917 TT genotypes were much more frequently found in patients with sustained virological response compared to null responders 38%,44% and 50% vs 2%,8.2% and 8.2%,respectively.These differences were highly significant in all three cases(P < 0.0001.CONCLUSION:The three IL28B polymorphisms studied are strongly associated with sustained virological response to therapy in Chilean patients with chronic CV genotype 1.
基金Supported by Scientific Research Fund of Shanghai Municipal Health Commission,No.201640368National Natural Science Foundation of China,No.81741103The Shanghai Plan for Women and Children's Health Service Capacity Construction(Enhancing the Service Capacity of Shanghai Women and Children Health Care Institutions).
文摘BACKGROUND Infantile-onset inflammatory bowel disease(IO-IBD)occurs in very young children and causes severe clinical manifestations,which has poor responses to traditional inflammatory bowel disease(IBD)treatments.At present,there are no simple and reliable laboratory indicators for early screening IO-IBD patients,especially those in whom the disease is caused by monogenic diseases.AIM To search for valuable indicators for early identifying IO-IBD patients,especially those in whom the disease is caused by monogenic diseases.METHODS A retrospective analysis was performed in 73 patients with IO-IBD admitted to our hospital in the past 5 years.Based on the next-generation sequencing results,they were divided into a monogenic IBD group(M-IBD)and a non-monogenic IBD group(NM-IBD).Forty age-matched patients with allergic proctocolitis(AP)were included in a control group.The clinical manifestations and the inflammatory factors in peripheral blood were evaluated.Logistic regression analysis and receiver operating characteristic(ROC)curve analysis were used to identify the screening factors and cut-off values of IO-IBD as well as monogenic IO-IBD,respectively.RESULTS Among the 44 M-IBD patients,35 carried IL-10RA mutations,and the most common mutations were c.301C>T(p.R101W,30/70)and the c.537G>A(p.T179T,17/70).Patients with higher serum tumor necrosis factor(TNF)-αvalue were more likely to have IBD[odds ratio(OR)=1.25,95%confidence interval(CI):1.05-1.50,P=0.013],while higher serum albumin level was associated with lower risk of IBD(OR=0.86,95%CI:0.74-1.00,P=0.048).The cut-off values of TNF-αand albumin were 17.40 pg/mL(sensitivity:0.78;specificity:0.88)and 36.50 g/L(sensitivity:0.80;specificity:0.90),respectively.The increased ferritin level was indicative of a genetic mutation in IO-IBD patients.Its cut-off value was 28.20 ng/mL(sensitivity:0.93;specificity:0.92).When interleukin(IL)-10 level was higher than 33.05 pg/mL(sensitivity:1.00;specificity:0.84),or the onset age was earlier than 0.21 mo(sensitivity:0.82;specificity:0.94),the presence of diseasecausing mutations in IL-10RA in IO-IBD patients was strongly suggested.CONCLUSION Serum TNF-αand albumin level could differentiate IO-IBD patients from allergic proctocolitis patients,and serum ferritin and IL-10 levels are useful indicators for early diagnosing monogenic IO-IBD.
文摘The controllable molecular weight and polydispersity of polyacrylonitriles(PANs) were synthesized in ionic liquids(ILs) by atom transfer radical polymerization(ATRP) using ethyl-2 bromopropionate(EPN-Br) as initiator and CuBr/pentamethyldiethylenetria-mine(PMDETA) as catalyst.The polydispersity index(PDI) in the ATRP of acrylonitriel(AN) in ILs is lower than 1.3.From the polymerization kinetics,plots of ln([M]0/[M]) with time show a linear dependence at the early stage of polymerization while the deviation is observed with the conversion rate increasing.The polymerization rate in ILs,especially in 1-butyl-3-methylimidazolium chloride([bmim]Cl),is higher than that in N,N-dimethylformamide(DMF).The polymerization rate increased and the average molecular weight decreased with temperature increasing.The polymerization rate,molecular weight and PDI varied with the variation of [AN]0:[EPN-Br]0:[CuBr]0:[PMDETA]0 ratio in the experimental range,the polymerization of AN in [bmim]Cl displayed living polymerization characteristics.Moreover,the catalyst and ILs are easily isolated from the polymer and reused.
文摘Background: Genetic variation influencing individual susceptibility to chemical carcinogens is one of the main factors leading to cancer development. The glutathione S-transferases (GSTs) are a family of enzymes belonging to phase II enzymes involved in detoxification of xenobiotics. A significant relationship is observed between the risk of developing cancer and genetic polymorphisms within GSTs. Methods: In this study, we investigated the influence of inherited GSTP1 (Ile105Val) gene polymorphism on the susceptibility to CML in Egypt in 40 CML patients (20 children and 20 adults), together with 40 healthy controls using a [PCR-RFLP] assay. Results: We found that the mutant type (IIe/Val, Val/Val) was significantly higher in CML patients (67.5%) compared to controls (35%) (p = 0.004);[odds ratio 3.9;95% CI: 1.5 - 9.7]. The mutant type was associated with more advanced phases in disease and with both worse hematological and cytogenetic responses when compared to the wild type (p = 0.03, p = 0.05, and p 0.001, respectively). Conclusion: GSTP1 (Ile105Val) gene polymorphism conferred a significant association with increased risk of CML and is associated with worse prognosis. Further studies on the functional consequences of this genetic polymorphism would pave the way to declare its role in the pathogenesis of CML or as a possible predictor for response to therapy.
文摘Among patients with familial amyloid polyneuropathy (FAP), those with transth yretin Val30Met mainly show distally predominant weakness and atrophy, whereas s ome FAP patients, including those with transthyretin Ser50Ile and Tyr114Cys, sho w muscle weakness and atrophy that is dominant proximally, simulating myopathy. To clarify the cause of proximally dominant muscular atrophy in patients with FA P transthyretin Ser50Ile and Tyr114Cys, we investigated the distinctive features of muscle specimens of patients with FAP, 3 of who had Val30Met, 2 Ser50Ile, an d 2 Tyr114Cys transthyretin. All specimens showed transthyretin amyloid around b lood vessels and perimysium, and neurogenic denervation patterns. The amount of amyloid around the vessels was much greater in patients with FAP Ser50Ile and Ty r114Cys than in Val30Met patients. Muscular amyloid angiopathy may contribute to motor nerve injury that, in turn, may lead to amyotropic changes in patients wi th FAP Ser50Ile and Tyr114Cys.
