Objective: To observe the clinical effect of Fifty ying acupuncture therapy for treatment of chronic bronchitis, and to explore its underlying mechanisms. Methods: A total of 36 out and in patients with chronic bronch...Objective: To observe the clinical effect of Fifty ying acupuncture therapy for treatment of chronic bronchitis, and to explore its underlying mechanisms. Methods: A total of 36 out and in patients with chronic bronchitis including 10 cases of asthma were subjected into this study. Among them, 20 cases were qi deficiency type and 16 qi yin deficiency with phlegm damp type. The changes of symptoms, T lymphocyte subgroups of CD 3 +, CD 4 +, CD 8 +, CD 4 +/CD 8 +, soluble interleuki 2 receptor (SIL 2 R), interleukin 6(IL 6), IgG, IgA and IgM before and after treatment were observed and detected using monoclonal indirect fluorescence method, enzyme linked immunosorbent assay (ELISA) and nephelometry respectively. Results: (1)The effective rate of qi dificiency type was 100% and that of qi yin deficiency with phlegm damp type 87.5%. (2) After acupuncture treatment, in qi deficiency type patients, serum IgG, IgA and IgM contents increased significantly (P<0.001) in comparison with pre treatment and in qi yin deficiency with phlegm damp type patients, IgG level lowered obviously. Serum SIL 2R and CD 4 +/CD 8 + decreased obviously (P<0.01, 0.001), CD 3 +, CD 4 + and CD 8 + increased evidently (P<0.01). Conclusion: The Fifty ying acupuncture therapy can better clinical symptoms of chronic bronchitis patients through its resultant up regulation of the immune system function.展开更多
Objective:Pancreatic cancer is one of the most aggressive malignancies,a robust prognostic signature and novel biomarkers are urgently needed for accurate stratification of the patients and optimization of clinical de...Objective:Pancreatic cancer is one of the most aggressive malignancies,a robust prognostic signature and novel biomarkers are urgently needed for accurate stratification of the patients and optimization of clinical decision-making.Methods:A list of bioinformatic analysis were applied in public dataset to construct an immune-related signature.Furthermore,the most pivotal gene in the signature was identified.The potential mechanism of the core gene function was revealed through GSEA,CIBERSORT,ESTIMATE,immunophenoscore(IPS)algorithm,single-cell analysis,and functional experiment.Results:An immune-related prognostic signature and associated nomogram were constructed and validated.Among the genes constituting the signature,interleukin 1 receptor type II(IL1R2)was identified as the gene occupying the most paramount position in the risk signature.Meanwhile,knockdown of IL1R2 significantly inhibited the proliferation,invasion,and migration ability of pancreatic cancer cells.Additionally,high IL1R2 expression was associated with reduced CD8+T cell infiltration in pancreatic cancer microenvironment,which may be due to high programmed cell death-ligand-1(PD-L1)expression in cancer cells.Finally,the IPS algorithm proved that patients with high IL1R2 expression possessed a higher tumor mutation burden and a higher probability of benefiting from immunotherapy.Conclusion:In conclusion,our study constructed an efficient immune-related prognostic signature and identified the key role of IL1R2 in the development of pancreatic cancer,as well as its potential to serve as a biomarker for immunotherapy efficacy prediction for pancreatic cancer.展开更多
文摘Objective: To observe the clinical effect of Fifty ying acupuncture therapy for treatment of chronic bronchitis, and to explore its underlying mechanisms. Methods: A total of 36 out and in patients with chronic bronchitis including 10 cases of asthma were subjected into this study. Among them, 20 cases were qi deficiency type and 16 qi yin deficiency with phlegm damp type. The changes of symptoms, T lymphocyte subgroups of CD 3 +, CD 4 +, CD 8 +, CD 4 +/CD 8 +, soluble interleuki 2 receptor (SIL 2 R), interleukin 6(IL 6), IgG, IgA and IgM before and after treatment were observed and detected using monoclonal indirect fluorescence method, enzyme linked immunosorbent assay (ELISA) and nephelometry respectively. Results: (1)The effective rate of qi dificiency type was 100% and that of qi yin deficiency with phlegm damp type 87.5%. (2) After acupuncture treatment, in qi deficiency type patients, serum IgG, IgA and IgM contents increased significantly (P<0.001) in comparison with pre treatment and in qi yin deficiency with phlegm damp type patients, IgG level lowered obviously. Serum SIL 2R and CD 4 +/CD 8 + decreased obviously (P<0.01, 0.001), CD 3 +, CD 4 + and CD 8 + increased evidently (P<0.01). Conclusion: The Fifty ying acupuncture therapy can better clinical symptoms of chronic bronchitis patients through its resultant up regulation of the immune system function.
基金supported by the CAMS Innovation Fund for Medical Sciences(2021,2021-1-I2M-002,to YZ)National Nature Science Foundation of China(2021,82102810,to CW)+2 种基金fellowship of China Postdoctoral Science Foundation(2022,2022T150067,to CW)National High Level Hospital Clinical Research Funding(2022,2022-PUMCH-D-001,to YZ)National Multidisciplinary Cooperative Diagnosis and Treatment Capacity Building Project for Major Diseases.
文摘Objective:Pancreatic cancer is one of the most aggressive malignancies,a robust prognostic signature and novel biomarkers are urgently needed for accurate stratification of the patients and optimization of clinical decision-making.Methods:A list of bioinformatic analysis were applied in public dataset to construct an immune-related signature.Furthermore,the most pivotal gene in the signature was identified.The potential mechanism of the core gene function was revealed through GSEA,CIBERSORT,ESTIMATE,immunophenoscore(IPS)algorithm,single-cell analysis,and functional experiment.Results:An immune-related prognostic signature and associated nomogram were constructed and validated.Among the genes constituting the signature,interleukin 1 receptor type II(IL1R2)was identified as the gene occupying the most paramount position in the risk signature.Meanwhile,knockdown of IL1R2 significantly inhibited the proliferation,invasion,and migration ability of pancreatic cancer cells.Additionally,high IL1R2 expression was associated with reduced CD8+T cell infiltration in pancreatic cancer microenvironment,which may be due to high programmed cell death-ligand-1(PD-L1)expression in cancer cells.Finally,the IPS algorithm proved that patients with high IL1R2 expression possessed a higher tumor mutation burden and a higher probability of benefiting from immunotherapy.Conclusion:In conclusion,our study constructed an efficient immune-related prognostic signature and identified the key role of IL1R2 in the development of pancreatic cancer,as well as its potential to serve as a biomarker for immunotherapy efficacy prediction for pancreatic cancer.
