Nowadays great progress has been made in targeted therapy and comprehensive therapy for a few cancers. But the prognosis of the vast majority of cancer patients is still weakness. It has been proven that inflammation ...Nowadays great progress has been made in targeted therapy and comprehensive therapy for a few cancers. But the prognosis of the vast majority of cancer patients is still weakness. It has been proven that inflammation can affect the development and treatment of cancer. Some previous studies have suggested that inflammation-related interleukins (ILs) play a significant role in the development of some cancers.1,2 ILs act as a considerable role in immunity,3 and inflammation affects the development of cancer. Therefore, the expression profile of IL gene may also be one of the vital markers of early pathological changes, occurrence and progression of cancer.4,5 Currently, studies on the expression of IL27 in cancer are still extremely limited. Our work comprehensively and systematically studied the IL27 gene expression in the generic cancer when compared with the existing IL27 gene related research experiment. We screened out cancer species linked with poor prognosis, and explored the correlation between IL27 gene expression and immune cell infiltration and immune microenvironment in these cancers further, providing an increasing number of the direct basis for subsequent studies. Our findings showed that IL27 can be used to assess prognosis and immune infiltration in patients with colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), head and neck squamous cell carcinoma (HNSC), skin cutaneous melanoma (SKCM) and testicular germ cell tumors (TGCT) as a biomarker.展开更多
目的研究黄芪多糖(APS)对人外周血单核细胞(PBMC)源性树突状细胞(DCs)的基因表达和其功能变化的影响,进一步探讨黄芪多糖的抗AS的作用机制。方法以健康人外周血分离PBMC源性DCs和血清作为研究对象,培育5 d后,随机分为APS组和对照组。其...目的研究黄芪多糖(APS)对人外周血单核细胞(PBMC)源性树突状细胞(DCs)的基因表达和其功能变化的影响,进一步探讨黄芪多糖的抗AS的作用机制。方法以健康人外周血分离PBMC源性DCs和血清作为研究对象,培育5 d后,随机分为APS组和对照组。其中APS组给予200 mg/L APS孵育过夜,对照组不给干预。通过基因芯片和RT-PCR技术,观察APS处理PBMC源性DCs的免疫功能和其他基因表达的差异与AS发生发展的关系。结果与对照组相比,APS组CD36(0.97±0.23 vs5.45±1.14)、IL-27(1.08±0.22 vs 2.97±0.61)基因表达相对量显著性上调;APS组IFI16(0.98±0.18 vs 0.46±0.11)基因表达相对量显著性下调。结论适量的APS可以上调DCs膜表面与抗原递呈相关的CD36、IL-27的表达,下调IFI16的表达,对增加DCs的免疫活性,促进DCs的成熟与分化有显著的影响。APS对AS的发生发展具有明显的积极地干预作用,有着重要的积极地临床意义。展开更多
文摘Nowadays great progress has been made in targeted therapy and comprehensive therapy for a few cancers. But the prognosis of the vast majority of cancer patients is still weakness. It has been proven that inflammation can affect the development and treatment of cancer. Some previous studies have suggested that inflammation-related interleukins (ILs) play a significant role in the development of some cancers.1,2 ILs act as a considerable role in immunity,3 and inflammation affects the development of cancer. Therefore, the expression profile of IL gene may also be one of the vital markers of early pathological changes, occurrence and progression of cancer.4,5 Currently, studies on the expression of IL27 in cancer are still extremely limited. Our work comprehensively and systematically studied the IL27 gene expression in the generic cancer when compared with the existing IL27 gene related research experiment. We screened out cancer species linked with poor prognosis, and explored the correlation between IL27 gene expression and immune cell infiltration and immune microenvironment in these cancers further, providing an increasing number of the direct basis for subsequent studies. Our findings showed that IL27 can be used to assess prognosis and immune infiltration in patients with colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), head and neck squamous cell carcinoma (HNSC), skin cutaneous melanoma (SKCM) and testicular germ cell tumors (TGCT) as a biomarker.
文摘目的研究黄芪多糖(APS)对人外周血单核细胞(PBMC)源性树突状细胞(DCs)的基因表达和其功能变化的影响,进一步探讨黄芪多糖的抗AS的作用机制。方法以健康人外周血分离PBMC源性DCs和血清作为研究对象,培育5 d后,随机分为APS组和对照组。其中APS组给予200 mg/L APS孵育过夜,对照组不给干预。通过基因芯片和RT-PCR技术,观察APS处理PBMC源性DCs的免疫功能和其他基因表达的差异与AS发生发展的关系。结果与对照组相比,APS组CD36(0.97±0.23 vs5.45±1.14)、IL-27(1.08±0.22 vs 2.97±0.61)基因表达相对量显著性上调;APS组IFI16(0.98±0.18 vs 0.46±0.11)基因表达相对量显著性下调。结论适量的APS可以上调DCs膜表面与抗原递呈相关的CD36、IL-27的表达,下调IFI16的表达,对增加DCs的免疫活性,促进DCs的成熟与分化有显著的影响。APS对AS的发生发展具有明显的积极地干预作用,有着重要的积极地临床意义。
