[Objectives]To explore the therapeutic effects of doxofylline combined with Erchen decoction in chronic obstructive pulmonary disease(COPD)model rats and its impact on serum IL-6 levels.[Methods]Rats were divided into...[Objectives]To explore the therapeutic effects of doxofylline combined with Erchen decoction in chronic obstructive pulmonary disease(COPD)model rats and its impact on serum IL-6 levels.[Methods]Rats were divided into seven groups:blank,model,Erchen decoction,doxofylline,and combined medication groups at low,medium,and high doses.After successful modeling,they were administrated intragastrically at the respective doses for 4 weeks.[Results]Compared to the control group,serum IL-6 levels in the Erchen decoction group and the combined medication groups at low and medium doses were significantly decreased(P<0.05).Additionally,the body weight of rats after treatment was significantly increased compared to before treatment(P<0.05).[Conclusions]Doxofylline combined with Erchen decoction can reduce serum IL-6 levels in COPD rats,improve their body weight,and exert a therapeutic effect on COPD.展开更多
Objective: To investigate the clinical significance of serum tumor markers CA153, CA125, CA72-4 and FIB, IL-6 levels in the detection of ovarian cancer. Methods: A total of 40 patients with ovarian carcinoma treated i...Objective: To investigate the clinical significance of serum tumor markers CA153, CA125, CA72-4 and FIB, IL-6 levels in the detection of ovarian cancer. Methods: A total of 40 patients with ovarian carcinoma treated in our hospital from September 2015 to June 2017 were selected as the ovarian cancer group;40 patients with benign ovarian tumors in the same period were selected as the benign ovarian tumor group;40 healthy subjects were selected as the control group. The levels of tumor markers CA153, CA125, CA72-4 and FIB, IL-6 were compared between the three groups. Results: The CA153, CA125, CA72-4 and FIB, IL-6 levels of the ovarian cancer group were significantly higher than those in benign ovarian tumor group and the control group;these levels in the benign ovarian tumor group was not significantly different from those in the control group. With the increase of clinical phase, the levels of CA153, CA125 and CA72-4 in patients with ovarian cancer were gradually increased, and the levels in phase Ⅲ and Ⅳ were significantly higher than those in phase Ⅱ and in phase I;and the CA125 in phase Ⅱ was significantly higher than that in phase Ⅰ. The levels of FIB and IL-6 in phase Ⅱ and in phase Ⅲ, Ⅳ were significantly higher than those in phase I;the IL-6 level in phase Ⅲ, Ⅳ was significantly higher than that in phase Ⅱ. Conclusion: Serum tumor markers CA153, CA125, CA72-4 and FIB, IL-6 levels for ovarian cancer detection can be helpful for clinical diagnosis and worthy of promotion.展开更多
Purpose: This study was conducted to detect IL-6 in synovial fluid in cases with TMJ internal derangement. Patients & Methods: This study was conducted on forty patients ASA class I with TMJ Internal derangement. ...Purpose: This study was conducted to detect IL-6 in synovial fluid in cases with TMJ internal derangement. Patients & Methods: This study was conducted on forty patients ASA class I with TMJ Internal derangement. All patients had been subjected to arthrocentesis. The synovial fluid was collected before wash and lavage was done for the affected joint. Then, the selected patients were divided randomly into two equal groups, group I: consisted of 20 patients where arthrocentesis was performed for the affected joint followed by intraarticular injection of one ml, tramadol hydrochloride. Group II: consisted of 20 patients where arthrocentesis was performed for the affected joint followed by intraarticular injection of one ml. sodium hyaluronate. Another synovial fluid sample was aspirated after one month. The interlieukin-6 receptors in the aspirated synovial fluid were measured using humans IL-6 enzyme-linked immunosorbent assay. Results: IL-6 was detected in the synovial fluid of joints with internal derangement. During follow up assessment of IL-6, the maximum decrease in IL-6 level was in the patients of group I who subjected to arthrocentesis with tramadol injection, as the mean IL-6 was (4.93 ± 1.36) followed by the patients of group II who subjected to arthrocentesis with sodium hyaluronate injection where the mean IL-6 level was (6.88 ± 1.76). There were significant p-value = (P = 0.000). Conclusions: It had been concluded that the detection of IL-6 in the synovial fluid of joints with internal derangement considered an indicator for inflammatory reaction in the joint and also the efficacy of arthrocentesis with tramadol suggested its anti-inflammatory effect.展开更多
Objective To investigate the changes of the concentration of interkeukine β 1(IL-1β) and Interleukine 6(IL-6) in the serum of the people who live in the different altitude,and try to find the relation between the cy...Objective To investigate the changes of the concentration of interkeukine β 1(IL-1β) and Interleukine 6(IL-6) in the serum of the people who live in the different altitude,and try to find the relation between the cytokines(CK) and the hypoxia adaptation and some diseases caused by hypoxia.Methods Using enzyme linked immunoabsorbent assay(ELISA) to detect the level of the concentration of IL-1β and IL-6 in the serum.Results The concentration of IL-1β showed significant increase in values(P<0.05) at 4050m.IL-6 in same altitude showed obvious changes as well(P<0.05).Conclusion The results showed that the concentration of IL-1β and IL-6 have significant changes in the serum.This means that they may be involved in the process of the adaptation and some sickness because of hypoxia.展开更多
Objectives:Non-small cell lung cancer(NSCLC)remains a leading cause of cancer-related mortality,with limited understanding of lncRNA-driven mechanisms in tumor progression.This study aimed to identify differentially e...Objectives:Non-small cell lung cancer(NSCLC)remains a leading cause of cancer-related mortality,with limited understanding of lncRNA-driven mechanisms in tumor progression.This study aimed to identify differentially expressed lncRNAs in NSCLC tissues and elucidate the functional role of the significantly upregulated RP3-340N1.2 in promoting malignancy.Methods:RNA sequencing was used to screen dysregulated lncRNAs.RP3-340N1.2 was functionally characterized via gain/loss-of-function assays in NSCLC cells,assessing proliferation,migration,and macrophage polarization.Mechanisms of interleukin 6(IL-6)regulation were explored using cytokine profiling,Actinomycin D assays,and RNA Immunoprecipitation(RIP)assays to study RP3-340N1.2 interactions with zinc finger CCCH-type containing 12A(ZC3H12A)and IL-6 mRNA.Results:RP3-340N1.2 was upregulated in NSCLC tissues and cells.Functional assays demonstrated that RP3-340N1.2 knockdown suppressed NSCLC cell proliferation/migration and reduced macrophage polarization toward tumor-associated phenotypes.Mechanistically,RP3-340N1.2 knockdown promoted IL-6 mRNA degradation,as supported by reduced IL-6 levels and accelerated mRNA decay.Further RIP assays revealed that RP3-340N1.2 interacts with ZC3H12A,an RNA-binding protein previously reported to degrade IL-6 mRNA,and that RP3-340N1.2 knockdown enhanced ZC3H12A binding to IL-6 mRNA.Consequently,RP3-340N1.2 knockdown in carcinoma cells attenuated IL-6-mediated tumor-promoting effects,including tumor cell proliferation and migration.Importantly,these effectswere observed not only in a direct carcinoma cell culturing system but also when carcinoma cells were exposed to conditioned medium from co-culturing RP3-340N1.2-knockdown tumor cells andmacrophages.Conclusion:RP3-340N1.2 drivesNSCLC malignancy by stabilizing IL-6 mRNA;its inhibition offers a potential therapeutic strategy to disrupt tumor-promoting interactions.展开更多
基金Supported by National and Autonomous Regional Undergraduate Innovation and Entrepreneurship Training Program(202310599008,S202310599099)Open Fund Project of Guangxi Universities for the Key Laboratory of Characteristic Ethnic Medicine in the Youjiang River Basin(yykf2024-06).
文摘[Objectives]To explore the therapeutic effects of doxofylline combined with Erchen decoction in chronic obstructive pulmonary disease(COPD)model rats and its impact on serum IL-6 levels.[Methods]Rats were divided into seven groups:blank,model,Erchen decoction,doxofylline,and combined medication groups at low,medium,and high doses.After successful modeling,they were administrated intragastrically at the respective doses for 4 weeks.[Results]Compared to the control group,serum IL-6 levels in the Erchen decoction group and the combined medication groups at low and medium doses were significantly decreased(P<0.05).Additionally,the body weight of rats after treatment was significantly increased compared to before treatment(P<0.05).[Conclusions]Doxofylline combined with Erchen decoction can reduce serum IL-6 levels in COPD rats,improve their body weight,and exert a therapeutic effect on COPD.
文摘Objective: To investigate the clinical significance of serum tumor markers CA153, CA125, CA72-4 and FIB, IL-6 levels in the detection of ovarian cancer. Methods: A total of 40 patients with ovarian carcinoma treated in our hospital from September 2015 to June 2017 were selected as the ovarian cancer group;40 patients with benign ovarian tumors in the same period were selected as the benign ovarian tumor group;40 healthy subjects were selected as the control group. The levels of tumor markers CA153, CA125, CA72-4 and FIB, IL-6 were compared between the three groups. Results: The CA153, CA125, CA72-4 and FIB, IL-6 levels of the ovarian cancer group were significantly higher than those in benign ovarian tumor group and the control group;these levels in the benign ovarian tumor group was not significantly different from those in the control group. With the increase of clinical phase, the levels of CA153, CA125 and CA72-4 in patients with ovarian cancer were gradually increased, and the levels in phase Ⅲ and Ⅳ were significantly higher than those in phase Ⅱ and in phase I;and the CA125 in phase Ⅱ was significantly higher than that in phase Ⅰ. The levels of FIB and IL-6 in phase Ⅱ and in phase Ⅲ, Ⅳ were significantly higher than those in phase I;the IL-6 level in phase Ⅲ, Ⅳ was significantly higher than that in phase Ⅱ. Conclusion: Serum tumor markers CA153, CA125, CA72-4 and FIB, IL-6 levels for ovarian cancer detection can be helpful for clinical diagnosis and worthy of promotion.
文摘Purpose: This study was conducted to detect IL-6 in synovial fluid in cases with TMJ internal derangement. Patients & Methods: This study was conducted on forty patients ASA class I with TMJ Internal derangement. All patients had been subjected to arthrocentesis. The synovial fluid was collected before wash and lavage was done for the affected joint. Then, the selected patients were divided randomly into two equal groups, group I: consisted of 20 patients where arthrocentesis was performed for the affected joint followed by intraarticular injection of one ml, tramadol hydrochloride. Group II: consisted of 20 patients where arthrocentesis was performed for the affected joint followed by intraarticular injection of one ml. sodium hyaluronate. Another synovial fluid sample was aspirated after one month. The interlieukin-6 receptors in the aspirated synovial fluid were measured using humans IL-6 enzyme-linked immunosorbent assay. Results: IL-6 was detected in the synovial fluid of joints with internal derangement. During follow up assessment of IL-6, the maximum decrease in IL-6 level was in the patients of group I who subjected to arthrocentesis with tramadol injection, as the mean IL-6 was (4.93 ± 1.36) followed by the patients of group II who subjected to arthrocentesis with sodium hyaluronate injection where the mean IL-6 level was (6.88 ± 1.76). There were significant p-value = (P = 0.000). Conclusions: It had been concluded that the detection of IL-6 in the synovial fluid of joints with internal derangement considered an indicator for inflammatory reaction in the joint and also the efficacy of arthrocentesis with tramadol suggested its anti-inflammatory effect.
文摘Objective To investigate the changes of the concentration of interkeukine β 1(IL-1β) and Interleukine 6(IL-6) in the serum of the people who live in the different altitude,and try to find the relation between the cytokines(CK) and the hypoxia adaptation and some diseases caused by hypoxia.Methods Using enzyme linked immunoabsorbent assay(ELISA) to detect the level of the concentration of IL-1β and IL-6 in the serum.Results The concentration of IL-1β showed significant increase in values(P<0.05) at 4050m.IL-6 in same altitude showed obvious changes as well(P<0.05).Conclusion The results showed that the concentration of IL-1β and IL-6 have significant changes in the serum.This means that they may be involved in the process of the adaptation and some sickness because of hypoxia.
基金supported by the National Natural Science Foundation of China(No.81702296).
文摘Objectives:Non-small cell lung cancer(NSCLC)remains a leading cause of cancer-related mortality,with limited understanding of lncRNA-driven mechanisms in tumor progression.This study aimed to identify differentially expressed lncRNAs in NSCLC tissues and elucidate the functional role of the significantly upregulated RP3-340N1.2 in promoting malignancy.Methods:RNA sequencing was used to screen dysregulated lncRNAs.RP3-340N1.2 was functionally characterized via gain/loss-of-function assays in NSCLC cells,assessing proliferation,migration,and macrophage polarization.Mechanisms of interleukin 6(IL-6)regulation were explored using cytokine profiling,Actinomycin D assays,and RNA Immunoprecipitation(RIP)assays to study RP3-340N1.2 interactions with zinc finger CCCH-type containing 12A(ZC3H12A)and IL-6 mRNA.Results:RP3-340N1.2 was upregulated in NSCLC tissues and cells.Functional assays demonstrated that RP3-340N1.2 knockdown suppressed NSCLC cell proliferation/migration and reduced macrophage polarization toward tumor-associated phenotypes.Mechanistically,RP3-340N1.2 knockdown promoted IL-6 mRNA degradation,as supported by reduced IL-6 levels and accelerated mRNA decay.Further RIP assays revealed that RP3-340N1.2 interacts with ZC3H12A,an RNA-binding protein previously reported to degrade IL-6 mRNA,and that RP3-340N1.2 knockdown enhanced ZC3H12A binding to IL-6 mRNA.Consequently,RP3-340N1.2 knockdown in carcinoma cells attenuated IL-6-mediated tumor-promoting effects,including tumor cell proliferation and migration.Importantly,these effectswere observed not only in a direct carcinoma cell culturing system but also when carcinoma cells were exposed to conditioned medium from co-culturing RP3-340N1.2-knockdown tumor cells andmacrophages.Conclusion:RP3-340N1.2 drivesNSCLC malignancy by stabilizing IL-6 mRNA;its inhibition offers a potential therapeutic strategy to disrupt tumor-promoting interactions.
