BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA prog...BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA progression through the ERK1/2 signaling cascade.AIM To determine the mechanism of the IL-22/IL-22R1 pathway in CCA and provide new directions for its clinical treatment.METHODS IL-22R1 expression was assessed in human and rat CCA tissues utilizing immunohistochemical techniques,Western blot analysis,and quantitative reverse transcription PCR.The impact of IL-22 on CCA cells was assessed in vitro via tests for proliferation,migration,invasion,and apoptosis assays.The rat models of thioacetamide-induced CCA and subcutaneous xenografts in nude mice were used to assess the in vivo effects.ERK1/2 inhibitors were applied to elucidate the mechanistic role of the pathway.RESULTS IL-22R1 was overexpressed in CCA cell lines and tissues.IL-22 treatment increased the phosphorylation of ERK1/2,promoting tumor cell proliferation,migration,invasion,and resistance to apoptosis.ERK1/2 inhibition considerably reversed these effects both in vitro and in vivo.CONCLUSION The IL-22/IL-22R1 axis promotes CCA progression by activating ERK1/2 signaling.Targeting this pathway with ERK1/2 inhibitors offers potential therapeutic strategies for CCA.展开更多
结核病的防治是全世界关注的公共卫生问题,糖尿病会增加结核病的发病风险,故结核合并糖尿病是一个值得关注的重点问题,近年来,细胞因子与抗结核感染的免疫机制密切相关,其中尤以IL-22与结核病的免疫联系紧密,IL-22与结核合并糖尿病的免...结核病的防治是全世界关注的公共卫生问题,糖尿病会增加结核病的发病风险,故结核合并糖尿病是一个值得关注的重点问题,近年来,细胞因子与抗结核感染的免疫机制密切相关,其中尤以IL-22与结核病的免疫联系紧密,IL-22与结核合并糖尿病的免疫联系的研究也是值得重点关注的,本文拟从IL-22的来源、结构、信号通路及IL-22在结核中,在结核合并糖尿病中的相关机制及作用的研究进展做一综述,从几个方面一同来探讨IL-22对于结核合并糖尿病疾病中的研究意义和价值,讨论IL-22能否成为这一合并病的评估疾病病情程度及预后的观测指标之一。The prevention and control of tuberculosis is a public health issue of global concern. Diabetes increases the risk of tuberculosis, making the co-occurrence of tuberculosis and diabetes a significant issue worthy of attention. In recent years, cytokines have been closely related to the immune mechanisms against tuberculosis infection, particularly IL-22, which has a strong immunological connection with tuberculosis. The research on the immune relationship between IL-22 and tuberculosis complicated by diabetes is also deserving of special focus. This paper aims to provide a review of the research progress on IL-22, including its sources, structure, signaling pathways, and its related mechanisms and roles in tuberculosis and in tuberculosis complicated by diabetes. Several aspects will be explored to discuss the significance and value of IL-22 in the context of tuberculosis and diabetes co-occurrence, as well as to evaluate whether IL-22 could serve as a potential observational indicator for assessing disease severity and prognosis in this comorbidity.展开更多
基金National Natural Science Foundation of China,No.82372194.
文摘BACKGROUND IL-22 plays a pivotal role in the processes of inflammation and tissue healing.,but its role in cholangiocarcinoma(CCA)remains unclear.our study explored the IL-22/IL-22R1 pathway and its impact on CCA progression through the ERK1/2 signaling cascade.AIM To determine the mechanism of the IL-22/IL-22R1 pathway in CCA and provide new directions for its clinical treatment.METHODS IL-22R1 expression was assessed in human and rat CCA tissues utilizing immunohistochemical techniques,Western blot analysis,and quantitative reverse transcription PCR.The impact of IL-22 on CCA cells was assessed in vitro via tests for proliferation,migration,invasion,and apoptosis assays.The rat models of thioacetamide-induced CCA and subcutaneous xenografts in nude mice were used to assess the in vivo effects.ERK1/2 inhibitors were applied to elucidate the mechanistic role of the pathway.RESULTS IL-22R1 was overexpressed in CCA cell lines and tissues.IL-22 treatment increased the phosphorylation of ERK1/2,promoting tumor cell proliferation,migration,invasion,and resistance to apoptosis.ERK1/2 inhibition considerably reversed these effects both in vitro and in vivo.CONCLUSION The IL-22/IL-22R1 axis promotes CCA progression by activating ERK1/2 signaling.Targeting this pathway with ERK1/2 inhibitors offers potential therapeutic strategies for CCA.
文摘结核病的防治是全世界关注的公共卫生问题,糖尿病会增加结核病的发病风险,故结核合并糖尿病是一个值得关注的重点问题,近年来,细胞因子与抗结核感染的免疫机制密切相关,其中尤以IL-22与结核病的免疫联系紧密,IL-22与结核合并糖尿病的免疫联系的研究也是值得重点关注的,本文拟从IL-22的来源、结构、信号通路及IL-22在结核中,在结核合并糖尿病中的相关机制及作用的研究进展做一综述,从几个方面一同来探讨IL-22对于结核合并糖尿病疾病中的研究意义和价值,讨论IL-22能否成为这一合并病的评估疾病病情程度及预后的观测指标之一。The prevention and control of tuberculosis is a public health issue of global concern. Diabetes increases the risk of tuberculosis, making the co-occurrence of tuberculosis and diabetes a significant issue worthy of attention. In recent years, cytokines have been closely related to the immune mechanisms against tuberculosis infection, particularly IL-22, which has a strong immunological connection with tuberculosis. The research on the immune relationship between IL-22 and tuberculosis complicated by diabetes is also deserving of special focus. This paper aims to provide a review of the research progress on IL-22, including its sources, structure, signaling pathways, and its related mechanisms and roles in tuberculosis and in tuberculosis complicated by diabetes. Several aspects will be explored to discuss the significance and value of IL-22 in the context of tuberculosis and diabetes co-occurrence, as well as to evaluate whether IL-22 could serve as a potential observational indicator for assessing disease severity and prognosis in this comorbidity.
