目的分析脑脊液白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)对原发性眼内淋巴瘤(primary intraocular lymphoma,PIOL)患者预后的预测价值。方法采用回顾性研究方法,选取2018年1月~2022年12月中国医学科学院...目的分析脑脊液白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)对原发性眼内淋巴瘤(primary intraocular lymphoma,PIOL)患者预后的预测价值。方法采用回顾性研究方法,选取2018年1月~2022年12月中国医学科学院/北京协和医学院北京协和医院收治的60例PIOL患者,出院后随访2年进行预后评估,统计不良预后发生率。将发生不良预后的患者纳入预后不良组,将未发生不良预后的患者纳入预后良好组,对比两组入院时的临床资料。采用多因素Logistic回归分析评价脑脊液IL-6、IL-10对预后的影响。绘制受试者工作特征(receiver operating characteristic,ROC)曲线,分析脑脊液IL-6、IL-10对预后的预测价值。结果60例患者随访2年后,22例出现不良预后,不良预后发生率为36.67%。预后不良组22例,预后良好组38例;2组的脑脊液IL-6、IL-10比较,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,脑脊液IL-6、IL-10是PIOL患者不良预后的危险因素。脑脊液IL-6、IL-10联合检测对预后预测的曲线下面积(area under the curve,AUC)、敏感度、特异性分别为0.879、85.53%、91.46%,高于脑脊液IL-6、IL-10单项检测。结论脑脊液IL-6、IL-10对PIOL患者预后具有一定的预测价值,高IL-6、IL-10水平提示预后不良。展开更多
Objective:To evaluate the expression of IL-10 in mice immunized with a recombinant BCG expressing the MSP1C antigen(BCGMSP1C),in the presence and absence of a TLR4 agonist CRX-527.Methods:The BCG-MSP1C vaccine candida...Objective:To evaluate the expression of IL-10 in mice immunized with a recombinant BCG expressing the MSP1C antigen(BCGMSP1C),in the presence and absence of a TLR4 agonist CRX-527.Methods:The BCG-MSP1C vaccine candidate was cultured and characterized using acid-fast staining and field-emission scanning electron microscopy.The mice were immunized with BCG-MSP1C in the presence or absence of CRX-527 intraperitoneally.The expression and production of IL-10 were measured via real-time PCR and ELISA in peritoneal macrophages,spleen,liver,and lymph nodes of immunized mice.Results:IL-10 expression and production were significantly increased in peritoneal macrophages and lymph nodes in the presence of CRX-527.In contrast,spleen and liver samples showed a significant decrease in IL-10 levels with CRX-527.Conclusions:BCG-MSP1C shows promise as a malaria vaccine candidate by inducing IL-10 expression in multiple immune compartments and displaying stable morphology.Tissue-specific modulation of IL-10 by CRX-527 results in increased expression in the lymph nodes and peritoneal macrophages,and decreased levels in the liver and spleen.展开更多
文摘目的分析脑脊液白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)对原发性眼内淋巴瘤(primary intraocular lymphoma,PIOL)患者预后的预测价值。方法采用回顾性研究方法,选取2018年1月~2022年12月中国医学科学院/北京协和医学院北京协和医院收治的60例PIOL患者,出院后随访2年进行预后评估,统计不良预后发生率。将发生不良预后的患者纳入预后不良组,将未发生不良预后的患者纳入预后良好组,对比两组入院时的临床资料。采用多因素Logistic回归分析评价脑脊液IL-6、IL-10对预后的影响。绘制受试者工作特征(receiver operating characteristic,ROC)曲线,分析脑脊液IL-6、IL-10对预后的预测价值。结果60例患者随访2年后,22例出现不良预后,不良预后发生率为36.67%。预后不良组22例,预后良好组38例;2组的脑脊液IL-6、IL-10比较,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,脑脊液IL-6、IL-10是PIOL患者不良预后的危险因素。脑脊液IL-6、IL-10联合检测对预后预测的曲线下面积(area under the curve,AUC)、敏感度、特异性分别为0.879、85.53%、91.46%,高于脑脊液IL-6、IL-10单项检测。结论脑脊液IL-6、IL-10对PIOL患者预后具有一定的预测价值,高IL-6、IL-10水平提示预后不良。
基金supported by the Ministry of Higher Education Malaysia for Fundamental Research Grant Scheme with Project Code:FRGS/1/2021/SKK06/USM/002/4.
文摘Objective:To evaluate the expression of IL-10 in mice immunized with a recombinant BCG expressing the MSP1C antigen(BCGMSP1C),in the presence and absence of a TLR4 agonist CRX-527.Methods:The BCG-MSP1C vaccine candidate was cultured and characterized using acid-fast staining and field-emission scanning electron microscopy.The mice were immunized with BCG-MSP1C in the presence or absence of CRX-527 intraperitoneally.The expression and production of IL-10 were measured via real-time PCR and ELISA in peritoneal macrophages,spleen,liver,and lymph nodes of immunized mice.Results:IL-10 expression and production were significantly increased in peritoneal macrophages and lymph nodes in the presence of CRX-527.In contrast,spleen and liver samples showed a significant decrease in IL-10 levels with CRX-527.Conclusions:BCG-MSP1C shows promise as a malaria vaccine candidate by inducing IL-10 expression in multiple immune compartments and displaying stable morphology.Tissue-specific modulation of IL-10 by CRX-527 results in increased expression in the lymph nodes and peritoneal macrophages,and decreased levels in the liver and spleen.
