In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussiv...In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori.The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury(ALI).A lipopolysaccharide(LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting,co-immunoprecipitation,and quantitative real time-PCR analysis.The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA(Pla2g2a)-epidermal growth factor receptor(EGFR).The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation.In addition,M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and m TOR via the inhibition of Pla2g2a-EGFR.Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury,which may represent a promising strategy to treat ALI.展开更多
AIM: To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.METHODS: Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients witho...AIM: To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.METHODS: Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients without fatty liver and 20 normal controls. Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst I and Rsa I.RESULTS: The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6 %), obese fatty liver (38.5 %), or diabetic fatty liver (33.3 %) was significantly lower than that of the corresponding patients without fatty liver (100 %, 100 % and 80 % respectively), while the frequency of C2 genes, including C1/C2 and C2/C2, was significantly higher (71.4 %/0 %, 61.5 %/0 %, and 66.7 %/20 %) (P<0.01). The frequency distribution of the above genes of non-fatty liver patients (100 %/0, 100 %/0, and 80 %/20 %) was not significantly different from that of the normal controls (85 %/15 %) (P>0.05).CONCLUSION: The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver. Besides, C2 gene may play a key role in the pathogenesis of fatty liver.展开更多
The Department of Industrial Engineering of Tsinghua University won the Innovations in Curriculum Award of Institute of Industrial Engineering （IIE） at the annual meeting of IIE held in Nashville, Tennessee on May 2...The Department of Industrial Engineering of Tsinghua University won the Innovations in Curriculum Award of Institute of Industrial Engineering （IIE） at the annual meeting of IIE held in Nashville, Tennessee on May 20, 2007. The subject of the award-winning project is Logistics Laboratory. Competing with American universities, the project won the first place and became the only winner of this award. It is the first time for IIE to set up such an award. The Department of Industrial Engineering was established in 2001 with Dr. Gavriel Salvendy as the founding chair professor and department head. The Logistics Laboratory was set up in 2004.展开更多
针对传统的时频域故障诊断方法无法对故障实现自适应识别和分类,且准确率较低的问题,提出一种基于改进信息熵(improved information entropy,IIE)的长短时记忆网络(long-short time memory network,LSTM)方法。首先对原始信号分别进行...针对传统的时频域故障诊断方法无法对故障实现自适应识别和分类,且准确率较低的问题,提出一种基于改进信息熵(improved information entropy,IIE)的长短时记忆网络(long-short time memory network,LSTM)方法。首先对原始信号分别进行集合经验模态分解(ensemble empirical mode decomposition,EEMD)和变分模态分解(variational mode decomposition,VMD);将包含故障信息的所有本征模式分量(intrinsic mode functions,IMF)进行信息熵的求取;通过信息熵反映IMF的信息量和峭度指标对描述冲击成分的优势改进信息熵,构成特征向量;最后结合LSTM处理非线性数据的优势,利用组合特征训练LSTM网络建立诊断模型。实验结果表明:该方法能准确、高效地识别多种故障,准确率要比单一的EEMD-LSTM、VMD-LSTM、人工神经网络等传统方法更高。展开更多
基金the National Natural Science Foundation(81773982,82003937)Youth Academic leaders of the Qinglan Project in Jiangsu province for financial support。
文摘In the face of increasingly serious environmental pollution,the health of human lung tissues is also facing serious threats.Mogroside IIE(M2E)is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori.The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury(ALI).A lipopolysaccharide(LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting,co-immunoprecipitation,and quantitative real time-PCR analysis.The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA(Pla2g2a)-epidermal growth factor receptor(EGFR).The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation.In addition,M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and m TOR via the inhibition of Pla2g2a-EGFR.Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury,which may represent a promising strategy to treat ALI.
文摘AIM: To study the correlation between genetic polymorphism of cytochrome P450IIE1 (CYPIIE1) and fatty liver.METHODS: Peripheral blood mononuclear cells were collected in 56 patients with fatty liver, 26 patients without fatty liver and 20 normal controls. Then PCR-RFLP was used to analyze genetic polymorphism of CYPIIE1 in monocytes on the region of Pst I and Rsa I.RESULTS: The frequency of homozygotic C1 gene in patients with alcoholic fatty liver (28.6 %), obese fatty liver (38.5 %), or diabetic fatty liver (33.3 %) was significantly lower than that of the corresponding patients without fatty liver (100 %, 100 % and 80 % respectively), while the frequency of C2 genes, including C1/C2 and C2/C2, was significantly higher (71.4 %/0 %, 61.5 %/0 %, and 66.7 %/20 %) (P<0.01). The frequency distribution of the above genes of non-fatty liver patients (100 %/0, 100 %/0, and 80 %/20 %) was not significantly different from that of the normal controls (85 %/15 %) (P>0.05).CONCLUSION: The genetic polymorphism of CYPIIE1 on the position of Pst I and Rsa I is related to the susceptibility of fatty liver. Besides, C2 gene may play a key role in the pathogenesis of fatty liver.
文摘The Department of Industrial Engineering of Tsinghua University won the Innovations in Curriculum Award of Institute of Industrial Engineering （IIE） at the annual meeting of IIE held in Nashville, Tennessee on May 20, 2007. The subject of the award-winning project is Logistics Laboratory. Competing with American universities, the project won the first place and became the only winner of this award. It is the first time for IIE to set up such an award. The Department of Industrial Engineering was established in 2001 with Dr. Gavriel Salvendy as the founding chair professor and department head. The Logistics Laboratory was set up in 2004.
