实验采用末端快速扩增(RACE)技术从大菱鲆脾脏cDNA文库中筛选得到了免疫球蛋白轻链IgL的全长cD-NA片段。该序列包含47 bp的5′末端非编码区(5′UTR),738 bp的开放阅读框(ORF)和202 bp 3′UTR,整个开放阅读框编码246个氨基酸。系统发生...实验采用末端快速扩增(RACE)技术从大菱鲆脾脏cDNA文库中筛选得到了免疫球蛋白轻链IgL的全长cD-NA片段。该序列包含47 bp的5′末端非编码区(5′UTR),738 bp的开放阅读框(ORF)和202 bp 3′UTR,整个开放阅读框编码246个氨基酸。系统发生分析表明大菱鲆IgL基因与五条鰤的IgL基因起源关系最近。RT-PCR分析表明,大菱鲆IgL基因只在正常脾脏、肾脏和头肾组织中表达;IgL在大菱鲆胚胎发育细胞期就已开始表达,在大菱鲆胚胎尾芽期和体节期表达持续增强;在鳗弧菌感染大菱鲆脾脏和头肾早期均检测到IgL基因强烈表达,后期表达逐渐减弱;大菱鲆胚胎细胞(TEC)在用鳗弧菌感染48h后,IgL开始表达。这些结果均表明IgL基因在大菱鲆免疫应答中起着重要作用。展开更多
Logistics service providers significantly contribute to environmental degradation through improper waste disposal,hazardous packaging materials,excessive fuel consumption,and emissions.This study examines the impact o...Logistics service providers significantly contribute to environmental degradation through improper waste disposal,hazardous packaging materials,excessive fuel consumption,and emissions.This study examines the impact of green in-bound logistics and green outbound logistics on environmental,economic,and social performance of logistics companies using survey data from 221 Vietnamese logistics firms.Statistical analysis using Structural Equation Modeling revealed that green inbound logistics positively influences environmental and social performance while moderately affecting eco-nomic outcomes.In contrast,green outbound logistics demonstrates stronger effects on economic and environmental performance but exhibits limited impact on social dimensions.The measurement model showed strong reliability and validity(Cronbach's Alpha>0.70,robust Composite Reliability and Average Variance Extracted values),with excellent fit indices(Chi-Square/df=1.681,GFI=0.898,TLI=0.945,CFI=0.956,RMSEA=0.056).These findings highlight important distinctions between inbound and outbound green logistics impacts,offering valuable insights for an industry with currently low adoption rates of sustainable practices.The research demonstrates that implementing green logistics enhances both environmental preservation and business performance,providing compelling evidence for companies to accelerate their sustainability transition.By understanding these differential impacts,logistics firms can develop more tar-geted and effective sustainability strategies that optimize triple bottom line outcomes.展开更多
AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model...AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic reperfusion.After normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also measured.Other factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage.Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters.These benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 solution.In normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.展开更多
The intricately regulated differentiation of the somatic follicle cell lineages into distinct subpopulations with specific functions plays an essential role in Drosophila egg development. At early oogenesis, induction...The intricately regulated differentiation of the somatic follicle cell lineages into distinct subpopulations with specific functions plays an essential role in Drosophila egg development. At early oogenesis, induction of the stalk cells generates the first anteroposterior （AP） asymmetry in the egg chamber by inducing the posterior localization of the oocyte. Later, the properly specified posterior follicle cells signal to polarize the oocyte along the AP and dorsoventral （DV） axes at mid-oogenesis. Here, we show that lethal（2）giant larvae （lgl）, a Drosophila tumor suppressor gene, is required in the follicle cells for the differentiation of both stalk cells and posterior follicle cells. Loss-of-function mutations in lgl cause oocyte mispositioning in the younger one of the fused chambers, due to lack of the stalk. Removal oflgl function from the posterior follicle cells using the FLP/FRT system results in loss of the oocyte polarity that is elicited by the failure of those posterior cells to differentiate normally. Thus, we provide the first demonstration that lgl is implicated in the formation of the initial AP asymmetry and the patterning of the AP and DV axes in the oocyte by acting in the specification of a subset of somatic follicle cells.展开更多
文摘实验采用末端快速扩增(RACE)技术从大菱鲆脾脏cDNA文库中筛选得到了免疫球蛋白轻链IgL的全长cD-NA片段。该序列包含47 bp的5′末端非编码区(5′UTR),738 bp的开放阅读框(ORF)和202 bp 3′UTR,整个开放阅读框编码246个氨基酸。系统发生分析表明大菱鲆IgL基因与五条鰤的IgL基因起源关系最近。RT-PCR分析表明,大菱鲆IgL基因只在正常脾脏、肾脏和头肾组织中表达;IgL在大菱鲆胚胎发育细胞期就已开始表达,在大菱鲆胚胎尾芽期和体节期表达持续增强;在鳗弧菌感染大菱鲆脾脏和头肾早期均检测到IgL基因强烈表达,后期表达逐渐减弱;大菱鲆胚胎细胞(TEC)在用鳗弧菌感染48h后,IgL开始表达。这些结果均表明IgL基因在大菱鲆免疫应答中起着重要作用。
文摘Logistics service providers significantly contribute to environmental degradation through improper waste disposal,hazardous packaging materials,excessive fuel consumption,and emissions.This study examines the impact of green in-bound logistics and green outbound logistics on environmental,economic,and social performance of logistics companies using survey data from 221 Vietnamese logistics firms.Statistical analysis using Structural Equation Modeling revealed that green inbound logistics positively influences environmental and social performance while moderately affecting eco-nomic outcomes.In contrast,green outbound logistics demonstrates stronger effects on economic and environmental performance but exhibits limited impact on social dimensions.The measurement model showed strong reliability and validity(Cronbach's Alpha>0.70,robust Composite Reliability and Average Variance Extracted values),with excellent fit indices(Chi-Square/df=1.681,GFI=0.898,TLI=0.945,CFI=0.956,RMSEA=0.056).These findings highlight important distinctions between inbound and outbound green logistics impacts,offering valuable insights for an industry with currently low adoption rates of sustainable practices.The research demonstrates that implementing green logistics enhances both environmental preservation and business performance,providing compelling evidence for companies to accelerate their sustainability transition.By understanding these differential impacts,logistics firms can develop more tar-geted and effective sustainability strategies that optimize triple bottom line outcomes.
基金Supported by The Ministerio de de Sanidad y Consumo(PI081988)CIBER-EHD,Instituto Carlos Ⅲ,Madrid and Ministerio de Asuntos Exteriores y de Cooperación Internacionales(A/020255/08 and A/02987/09),Madrid
文摘AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic reperfusion.After normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also measured.Other factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage.Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters.These benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 solution.In normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.
基金We thank David Bilder （UC Berkeley, USA）, Michel Semeriva （IBDML, France）, Douglas A Harrison （University of Kentucky, USA）, Yuh Nung Jan （UCSF, USA）, Daniel St Johnston （University of Cambridge, UK）, Ruth Lehmann （NYU, USA）, Thomas S Hays （University of Minnesota, USA）, Anne Ephrussi （EMBL, Germany）, Zhaohui Wang （CAS, China）, the Bloomington Drosophila Stock Center and the Developmental Studies Hybridoma Bank for generously providing us with the fly strains and antibodies. We are also grateful to Qun Sun, Lingzhu Yu, Shunyan Weng, Ling Shen and other members of the Li Lab for technical assistance and discussions. This work was supported by National Basic Research Program of China （2007CB947300, 2007CB914504）, National Natural Science Foundation of China （30470890）, the Shanghai Pujiang Program （05PJ14075） and Shanghai Leading Academic Discipline Project （B205）.
文摘The intricately regulated differentiation of the somatic follicle cell lineages into distinct subpopulations with specific functions plays an essential role in Drosophila egg development. At early oogenesis, induction of the stalk cells generates the first anteroposterior （AP） asymmetry in the egg chamber by inducing the posterior localization of the oocyte. Later, the properly specified posterior follicle cells signal to polarize the oocyte along the AP and dorsoventral （DV） axes at mid-oogenesis. Here, we show that lethal（2）giant larvae （lgl）, a Drosophila tumor suppressor gene, is required in the follicle cells for the differentiation of both stalk cells and posterior follicle cells. Loss-of-function mutations in lgl cause oocyte mispositioning in the younger one of the fused chambers, due to lack of the stalk. Removal oflgl function from the posterior follicle cells using the FLP/FRT system results in loss of the oocyte polarity that is elicited by the failure of those posterior cells to differentiate normally. Thus, we provide the first demonstration that lgl is implicated in the formation of the initial AP asymmetry and the patterning of the AP and DV axes in the oocyte by acting in the specification of a subset of somatic follicle cells.
