目的明确免疫球蛋白G1重链(immunoglobulin G1 heavy chain,IGHG1)在胃癌诊断与预后评估中的作用及临床价值。方法通过生物信息学方法,分析IGHG1在恶性肿瘤组织中的表达情况。收集72例胃癌手术切除标本(癌旁组织作为对照),运用免疫组化...目的明确免疫球蛋白G1重链(immunoglobulin G1 heavy chain,IGHG1)在胃癌诊断与预后评估中的作用及临床价值。方法通过生物信息学方法,分析IGHG1在恶性肿瘤组织中的表达情况。收集72例胃癌手术切除标本(癌旁组织作为对照),运用免疫组化技术检测IGHG1蛋白在胃癌组织中的表达,并分析其表达的临床意义,明确其检测诊断胃癌的效能。收集60例胃癌患者术前和术后血清及60例健康体检者血清样本,采用ELISA技术检测患者外周血中IGHG1蛋白的浓度,分析其表达与胃癌的关系,构建受试者操作特征(receiver operating characteristic,ROC)曲线评估其检测的诊断价值。结果生物信息学分析结果显示,IGHG1在多种恶性肿瘤组织与癌旁组织中的表达存在显著差异(P<0.05)。免疫组化结果显示,胃癌组织中IGHG1表达较癌旁对照组织均显著上调(P<0.05)。IGHG1在正常黏膜与高、中、低分化胃腺癌组织中的阳性率依次为19.44%、25.00%、71.43%和93.62%,其表达水平与分化程度呈显著负相关(P<0.05),与淋巴结转移呈正相关(P<0.05)。ROC曲线评估显示,IGHG1检测灵敏度为0.83,特异度为0.75,曲线下面积(area under the curve,AUC)值为0.84。酶联免疫吸附试验结果显示,IGHG1在患者血清中的含量较对照组明显升高(P<0.05),其表达与患者年龄、TNM分期和组织学分级呈显著相关(P<0.05)。术后组IGHG1浓度回归基线水平(P=0.8304)。IGHG1诊断的灵敏度为0.97,特异性为0.75,AUC为0.91,IGHG1对应的最佳截断值为1.50μg/mL。结论IGHG1在胃癌组织及外周血清中均高表达,其表达与肿瘤的分化程度呈显著负相关,与TNM分期呈显著正相关,其可能是胃癌患者预后的评估指标之一,可能是胃癌诊断及预后评估的潜在标志物。展开更多
Objective： Ovarian cancer（OC） is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin γ-1 heavy chain constant region...Objective： Ovarian cancer（OC） is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin γ-1 heavy chain constant region（IGHG1） as its antigen. We explore the function of IGHG1 in proliferation, apoptosis and motility of OC cells further in this research.Methods： IGHG1 expression in OC specimens was detected through immunohistochemistry. Real-time quantitative polymerase chain reaction（RT-q PCR） or western blotting assay was used to test IGHG1 expression in OC cells. Viability of OC cells was tested by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay. Flow cytometry or western blotting assay was used to detect cell cycle and apoptosis. Cellular motility was analyzed by using transwell assay and the markers of epithelial-mesenchymal transition（EMT） were tested through immunoblots.Results： Although it exerts negligible effect on the viability and apoptosis of OC cells, IGHG1 could promote migration and invasion of malignant cells in vitro. Mechanistically, IGHG1 increases the expression of N-cadherin and Vimentin while decreases E-cadherin expression. Additionally, IGHG1 expression in OC specimens is higher relative to the paired normal counterparts. Further analysis demonstrates that the increased IGHG1 expression correlates positively with the lymph node metastasis of OC.Conclusions： IGHG1 promotes the motility of OC cells likely through executing the EMT program. Increased IGHG1 expression in OC specimens is associated with the lymph node metastasis.展开更多
文摘目的明确免疫球蛋白G1重链(immunoglobulin G1 heavy chain,IGHG1)在胃癌诊断与预后评估中的作用及临床价值。方法通过生物信息学方法,分析IGHG1在恶性肿瘤组织中的表达情况。收集72例胃癌手术切除标本(癌旁组织作为对照),运用免疫组化技术检测IGHG1蛋白在胃癌组织中的表达,并分析其表达的临床意义,明确其检测诊断胃癌的效能。收集60例胃癌患者术前和术后血清及60例健康体检者血清样本,采用ELISA技术检测患者外周血中IGHG1蛋白的浓度,分析其表达与胃癌的关系,构建受试者操作特征(receiver operating characteristic,ROC)曲线评估其检测的诊断价值。结果生物信息学分析结果显示,IGHG1在多种恶性肿瘤组织与癌旁组织中的表达存在显著差异(P<0.05)。免疫组化结果显示,胃癌组织中IGHG1表达较癌旁对照组织均显著上调(P<0.05)。IGHG1在正常黏膜与高、中、低分化胃腺癌组织中的阳性率依次为19.44%、25.00%、71.43%和93.62%,其表达水平与分化程度呈显著负相关(P<0.05),与淋巴结转移呈正相关(P<0.05)。ROC曲线评估显示,IGHG1检测灵敏度为0.83,特异度为0.75,曲线下面积(area under the curve,AUC)值为0.84。酶联免疫吸附试验结果显示,IGHG1在患者血清中的含量较对照组明显升高(P<0.05),其表达与患者年龄、TNM分期和组织学分级呈显著相关(P<0.05)。术后组IGHG1浓度回归基线水平(P=0.8304)。IGHG1诊断的灵敏度为0.97,特异性为0.75,AUC为0.91,IGHG1对应的最佳截断值为1.50μg/mL。结论IGHG1在胃癌组织及外周血清中均高表达,其表达与肿瘤的分化程度呈显著负相关,与TNM分期呈显著正相关,其可能是胃癌患者预后的评估指标之一,可能是胃癌诊断及预后评估的潜在标志物。
基金supported by Special Funds of the National Natural Science Foundation of China (No. 81341077)
文摘Objective： Ovarian cancer（OC） is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin γ-1 heavy chain constant region（IGHG1） as its antigen. We explore the function of IGHG1 in proliferation, apoptosis and motility of OC cells further in this research.Methods： IGHG1 expression in OC specimens was detected through immunohistochemistry. Real-time quantitative polymerase chain reaction（RT-q PCR） or western blotting assay was used to test IGHG1 expression in OC cells. Viability of OC cells was tested by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay. Flow cytometry or western blotting assay was used to detect cell cycle and apoptosis. Cellular motility was analyzed by using transwell assay and the markers of epithelial-mesenchymal transition（EMT） were tested through immunoblots.Results： Although it exerts negligible effect on the viability and apoptosis of OC cells, IGHG1 could promote migration and invasion of malignant cells in vitro. Mechanistically, IGHG1 increases the expression of N-cadherin and Vimentin while decreases E-cadherin expression. Additionally, IGHG1 expression in OC specimens is higher relative to the paired normal counterparts. Further analysis demonstrates that the increased IGHG1 expression correlates positively with the lymph node metastasis of OC.Conclusions： IGHG1 promotes the motility of OC cells likely through executing the EMT program. Increased IGHG1 expression in OC specimens is associated with the lymph node metastasis.
