AIM To investigate possible effects of IRF5 polymorphisms in the 3'UTR region of the IFR5 locus on susceptibilityto hepatitis B virus(HBV)infection and progression of liver diseases among clinically classified Vie...AIM To investigate possible effects of IRF5 polymorphisms in the 3'UTR region of the IFR5 locus on susceptibilityto hepatitis B virus(HBV)infection and progression of liver diseases among clinically classified Vietnamese patients.METHODS Four IFR5 SNPs(rs13242262 A/T,rs77416878 C/T,rs10488630 A/G,and rs2280714 T/C)were genotyped in clinically classified HBV patients[chronic hepatitis B(CHB).n=99;liver cirrhosis(LC),n=131;hepatocellular carcinoma(HCC),n=149]and in 242 healthy controls by direct sequencing and Taq Man realtime PCR assays.RESULTS Comparing patients and controls,no significant association was observed for the four IFR5 variants.However,the alleles rs13242262 T and rs10488630 G contributed to an increased risk of liver cirrhosis(LC vs CHB:OR=1.5,95%CI:1.1-2.3,adjusted P=0.04;LC vs CHB:OR=1.7,95%CI:1.1-2.6,adjusted P=0.019).Haplotype IRF5*TCGT constructed from 4 SNPs was observed frequently in LC compared to CHB patients(OR=2.1,95%CI:1.2-3.3,adjusted P=0.008).Haplotype IRF5*TCAT occurred rather among CHB patients than in the other HBV patient groups(LC vs CHB:OR=0.4,95%CI:0.2-0.8,adjusted P=0.03;HCC vs CHB:OR=0.3,95%CI:0.15-0.7,adjusted P=0.003).The IRF5*TCAT haplotype was also associated with increased levels of ALT,AST and bilirubin.CONCLUSION Our study shows that IFR5 variants may contribute as a host factor in determining the pathogenesis in chronic HBV infections.展开更多
基金Supported by NAFOSTED,No.108.02-2017.15BMBF,No.01DP17047
文摘AIM To investigate possible effects of IRF5 polymorphisms in the 3'UTR region of the IFR5 locus on susceptibilityto hepatitis B virus(HBV)infection and progression of liver diseases among clinically classified Vietnamese patients.METHODS Four IFR5 SNPs(rs13242262 A/T,rs77416878 C/T,rs10488630 A/G,and rs2280714 T/C)were genotyped in clinically classified HBV patients[chronic hepatitis B(CHB).n=99;liver cirrhosis(LC),n=131;hepatocellular carcinoma(HCC),n=149]and in 242 healthy controls by direct sequencing and Taq Man realtime PCR assays.RESULTS Comparing patients and controls,no significant association was observed for the four IFR5 variants.However,the alleles rs13242262 T and rs10488630 G contributed to an increased risk of liver cirrhosis(LC vs CHB:OR=1.5,95%CI:1.1-2.3,adjusted P=0.04;LC vs CHB:OR=1.7,95%CI:1.1-2.6,adjusted P=0.019).Haplotype IRF5*TCGT constructed from 4 SNPs was observed frequently in LC compared to CHB patients(OR=2.1,95%CI:1.2-3.3,adjusted P=0.008).Haplotype IRF5*TCAT occurred rather among CHB patients than in the other HBV patient groups(LC vs CHB:OR=0.4,95%CI:0.2-0.8,adjusted P=0.03;HCC vs CHB:OR=0.3,95%CI:0.15-0.7,adjusted P=0.003).The IRF5*TCAT haplotype was also associated with increased levels of ALT,AST and bilirubin.CONCLUSION Our study shows that IFR5 variants may contribute as a host factor in determining the pathogenesis in chronic HBV infections.
